This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 169.28 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 100,063 (1.9%) Reads that were too short: 40,622 (0.8%) Reads written (passing filters): 59,441 (1.1%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 11,379,334 bp (0.7%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 100063 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 347 1302.2 0 347 7 11097 1302.2 0 11097 8 64 1302.2 0 64 9 2734 1302.2 0 2734 10 19 1302.2 0 19 11 7 1302.2 0 7 12 11 1302.2 0 11 13 17 1302.2 0 17 14 18 1302.2 0 18 15 7 1302.2 0 7 16 24 1302.2 0 24 17 35 1302.2 0 35 18 17 1302.2 0 17 19 6 1302.2 0 6 20 9 1302.2 0 9 21 7 1302.2 0 7 22 12 1302.2 0 12 23 9 1302.2 0 9 24 9 1302.2 0 9 25 14 1302.2 0 14 26 17 1302.2 0 17 27 18 1302.2 0 18 28 15 1302.2 0 15 29 16 1302.2 0 16 30 23 1302.2 0 23 31 19 1302.2 0 19 32 7 1302.2 0 7 33 17 1302.2 0 17 34 12 1302.2 0 12 35 11 1302.2 0 11 36 16 1302.2 0 16 37 15 1302.2 0 15 38 7 1302.2 0 7 39 30 1302.2 0 30 40 23 1302.2 0 23 41 31 1302.2 0 31 42 23 1302.2 0 23 43 18 1302.2 0 18 44 136 1302.2 0 136 45 48 1302.2 0 48 46 24 1302.2 0 24 47 9 1302.2 0 9 48 8 1302.2 0 8 49 13 1302.2 0 13 50 18 1302.2 0 18 51 21 1302.2 0 21 52 15 1302.2 0 15 53 107 1302.2 0 107 54 10 1302.2 0 10 55 8 1302.2 0 8 56 104 1302.2 0 104 57 23 1302.2 0 23 58 51 1302.2 0 51 59 52 1302.2 0 52 60 23 1302.2 0 23 61 14 1302.2 0 14 62 8 1302.2 0 8 63 12 1302.2 0 12 64 5 1302.2 0 5 65 5 1302.2 0 5 66 16 1302.2 0 16 67 16 1302.2 0 16 68 20 1302.2 0 20 69 11 1302.2 0 11 70 5 1302.2 0 5 71 15 1302.2 0 15 72 9 1302.2 0 9 73 43 1302.2 0 43 74 181 1302.2 0 181 75 440 1302.2 0 440 76 33 1302.2 0 33 77 23 1302.2 0 23 78 48 1302.2 0 48 79 85 1302.2 0 85 80 86 1302.2 0 86 81 278 1302.2 0 278 82 310 1302.2 0 310 83 279 1302.2 0 279 84 160 1302.2 0 160 85 119 1302.2 0 119 86 107 1302.2 0 107 87 91 1302.2 0 91 88 98 1302.2 0 98 89 30 1302.2 0 30 90 128 1302.2 0 128 91 340 1302.2 0 340 92 253 1302.2 0 253 93 405 1302.2 0 405 94 277 1302.2 0 277 95 98 1302.2 0 98 96 202 1302.2 0 202 97 536 1302.2 0 536 98 207 1302.2 0 207 99 87 1302.2 0 87 100 29 1302.2 0 29 101 48 1302.2 0 48 102 51 1302.2 0 51 103 84 1302.2 0 84 104 209 1302.2 0 209 105 57 1302.2 0 57 106 519 1302.2 0 519 107 899 1302.2 0 899 108 1447 1302.2 0 1447 109 65 1302.2 0 65 110 74 1302.2 0 74 111 63 1302.2 0 63 112 93 1302.2 0 93 113 175 1302.2 0 175 114 289 1302.2 0 289 115 393 1302.2 0 393 116 559 1302.2 0 559 117 87 1302.2 0 87 118 99 1302.2 0 99 119 377 1302.2 0 377 120 254 1302.2 0 254 121 307 1302.2 0 307 122 223 1302.2 0 223 123 196 1302.2 0 196 124 243 1302.2 0 243 125 358 1302.2 0 358 126 183 1302.2 0 183 127 223 1302.2 0 223 128 493 1302.2 0 493 129 388 1302.2 0 388 130 425 1302.2 0 425 131 130 1302.2 0 130 132 114 1302.2 0 114 133 243 1302.2 0 243 134 511 1302.2 0 511 135 444 1302.2 0 444 136 362 1302.2 0 362 137 272 1302.2 0 272 138 462 1302.2 0 462 139 379 1302.2 0 379 140 386 1302.2 0 386 141 591 1302.2 0 591 142 1055 1302.2 0 1055 143 380 1302.2 0 380 144 207 1302.2 0 207 145 186 1302.2 0 186 146 242 1302.2 0 242 147 293 1302.2 0 293 148 1746 1302.2 0 1746 149 1001 1302.2 0 1001 150 1622 1302.2 0 1622 151 151 1302.2 0 151 152 153 1302.2 0 153 153 209 1302.2 0 209 154 289 1302.2 0 289 155 1450 1302.2 0 1450 156 422 1302.2 0 422 157 505 1302.2 0 505 158 178 1302.2 0 178 159 235 1302.2 0 235 160 333 1302.2 0 333 161 416 1302.2 0 416 162 425 1302.2 0 425 163 1401 1302.2 0 1401 164 689 1302.2 0 689 165 944 1302.2 0 944 166 515 1302.2 0 515 167 456 1302.2 0 456 168 360 1302.2 0 360 169 221 1302.2 0 221 170 192 1302.2 0 192 171 310 1302.2 0 310 172 236 1302.2 0 236 173 131 1302.2 0 131 174 152 1302.2 0 152 175 305 1302.2 0 305 176 179 1302.2 0 179 177 200 1302.2 0 200 178 148 1302.2 0 148 179 133 1302.2 0 133 180 575 1302.2 0 575 181 985 1302.2 0 985 182 929 1302.2 0 929 183 567 1302.2 0 567 184 546 1302.2 0 546 185 471 1302.2 0 471 186 238 1302.2 0 238 187 199 1302.2 0 199 188 202 1302.2 0 202 189 244 1302.2 0 244 190 163 1302.2 0 163 191 307 1302.2 0 307 192 333 1302.2 0 333 193 300 1302.2 0 300 194 222 1302.2 0 222 195 305 1302.2 0 305 196 255 1302.2 0 255 197 97 1302.2 0 97 198 94 1302.2 0 94 199 166 1302.2 0 166 200 486 1302.2 0 486 201 352 1302.2 0 352 202 239 1302.2 0 239 203 231 1302.2 0 231 204 485 1302.2 0 485 205 392 1302.2 0 392 206 274 1302.2 0 274 207 499 1302.2 0 499 208 350 1302.2 0 350 209 251 1302.2 0 251 210 398 1302.2 0 398 211 283 1302.2 0 283 212 478 1302.2 0 478 213 313 1302.2 0 313 214 363 1302.2 0 363 215 519 1302.2 0 519 216 528 1302.2 0 528 217 422 1302.2 0 422 218 324 1302.2 0 324 219 253 1302.2 0 253 220 430 1302.2 0 430 221 498 1302.2 0 498 222 144 1302.2 0 144 223 112 1302.2 0 112 224 376 1302.2 0 376 225 488 1302.2 0 488 226 758 1302.2 0 758 227 720 1302.2 0 720 228 529 1302.2 0 529 229 391 1302.2 0 391 230 262 1302.2 0 262 231 350 1302.2 0 350 232 785 1302.2 0 785 233 449 1302.2 0 449 234 242 1302.2 0 242 235 341 1302.2 0 341 236 430 1302.2 0 430 237 334 1302.2 0 334 238 172 1302.2 0 172 239 131 1302.2 0 131 240 191 1302.2 0 191 241 180 1302.2 0 180 242 206 1302.2 0 206 243 171 1302.2 0 171 244 336 1302.2 0 336 245 402 1302.2 0 402 246 499 1302.2 0 499 247 378 1302.2 0 378 248 336 1302.2 0 336 249 285 1302.2 0 285 250 312 1302.2 0 312 251 154 1302.2 0 154 252 129 1302.2 0 129 253 310 1302.2 0 310 254 814 1302.2 0 814 255 1048 1302.2 0 1048 256 821 1302.2 0 821 257 592 1302.2 0 592 258 595 1302.2 0 595 259 310 1302.2 0 310 260 188 1302.2 0 188 261 108 1302.2 0 108 262 144 1302.2 0 144 263 204 1302.2 0 204 264 168 1302.2 0 168 265 326 1302.2 0 326 266 577 1302.2 0 577 267 498 1302.2 0 498 268 862 1302.2 0 862 269 1395 1302.2 0 1395 270 525 1302.2 0 525 271 442 1302.2 0 442 272 366 1302.2 0 366 273 337 1302.2 0 337 274 203 1302.2 0 203 275 334 1302.2 0 334 276 358 1302.2 0 358 277 261 1302.2 0 261 278 256 1302.2 0 256 279 332 1302.2 0 332 280 540 1302.2 0 540 281 503 1302.2 0 503 282 325 1302.2 0 325 283 292 1302.2 0 292 284 317 1302.2 0 317 285 249 1302.2 0 249 286 490 1302.2 0 490 287 386 1302.2 0 386 288 347 1302.2 0 347 289 522 1302.2 0 522 290 377 1302.2 0 377 291 289 1302.2 0 289 292 350 1302.2 0 350 293 430 1302.2 0 430 294 793 1302.2 0 793 295 1610 1302.2 0 1610 296 765 1302.2 0 765 297 633 1302.2 0 633 298 515 1302.2 0 515 299 237 1302.2 0 237 300 115 1302.2 0 115 301 40 1302.2 0 40 Finished in 2.92 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 59,441 Reads with adapters: 2,780 (4.7%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 2,752 (4.6%) Total basepairs processed: 11,379,334 bp Total written (filtered): 745,948 bp (6.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2780 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 1 19 34 0.0 3 0 25 1 1 7 20 2670 0.0 4 2570 77 9 8 6 21 12 0.0 4 4 6 0 1 1 22 8 0.0 4 3 0 0 0 5 23 3 0.0 4 0 0 0 3 27 1 0.0 4 0 0 0 0 1 35 2 0.0 4 0 0 0 0 2 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 1 73 1 0.0 4 0 0 0 0 1 76 2 0.0 4 0 0 0 0 2 80 2 0.0 4 0 0 0 0 2 86 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 107 2 0.0 4 0 0 0 0 2 118 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 127 16 0.0 4 0 0 0 0 16 132 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 2,752 Reads with adapters: 231 (8.4%) Reads written (passing filters): 2,752 (100.0%) Total basepairs processed: 745,948 bp Total written (filtered): 742,266 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 231 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.8% C: 3.9% G: 89.2% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 76 0.0 0 5 71 10 45 0.0 1 4 41 11 104 0.0 1 0 104 115 1 0.0 1 0 1 257 4 0.0 1 4 261 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.