This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 169.19 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 90,128 (1.7%) Reads that were too short: 35,402 (0.7%) Reads written (passing filters): 54,726 (1.0%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 12,996,997 bp (0.8%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 90128 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 619 1302.2 0 619 7 14592 1302.2 0 14592 8 306 1302.2 0 306 9 9535 1302.2 0 9535 10 21 1302.2 0 21 11 10 1302.2 0 10 12 21 1302.2 0 21 13 17 1302.2 0 17 14 26 1302.2 0 26 15 22 1302.2 0 22 16 5066 1302.2 0 5066 17 191 1302.2 0 191 18 29 1302.2 0 29 19 19 1302.2 0 19 20 15 1302.2 0 15 21 15 1302.2 0 15 22 26 1302.2 0 26 23 14 1302.2 0 14 24 17 1302.2 0 17 25 31 1302.2 0 31 26 19 1302.2 0 19 27 18 1302.2 0 18 28 15 1302.2 0 15 29 22 1302.2 0 22 30 29 1302.2 0 29 31 23 1302.2 0 23 32 19 1302.2 0 19 33 22 1302.2 0 22 34 8 1302.2 0 8 35 17 1302.2 0 17 36 20 1302.2 0 20 37 13 1302.2 0 13 38 21 1302.2 0 21 39 16 1302.2 0 16 40 13 1302.2 0 13 41 23 1302.2 0 23 42 14 1302.2 0 14 43 24 1302.2 0 24 44 33 1302.2 0 33 45 13 1302.2 0 13 46 27 1302.2 0 27 47 15 1302.2 0 15 48 19 1302.2 0 19 49 34 1302.2 0 34 50 2637 1302.2 0 2637 51 141 1302.2 0 141 52 42 1302.2 0 42 53 26 1302.2 0 26 54 13 1302.2 0 13 55 17 1302.2 0 17 56 40 1302.2 0 40 57 10 1302.2 0 10 58 20 1302.2 0 20 59 20 1302.2 0 20 60 14 1302.2 0 14 61 21 1302.2 0 21 62 13 1302.2 0 13 63 12 1302.2 0 12 64 15 1302.2 0 15 65 15 1302.2 0 15 66 12 1302.2 0 12 67 31 1302.2 0 31 68 221 1302.2 0 221 69 95 1302.2 0 95 70 11 1302.2 0 11 71 42 1302.2 0 42 72 21 1302.2 0 21 73 16 1302.2 0 16 74 63 1302.2 0 63 75 37 1302.2 0 37 76 43 1302.2 0 43 77 19 1302.2 0 19 78 22 1302.2 0 22 79 17 1302.2 0 17 80 64 1302.2 0 64 81 137 1302.2 0 137 82 70 1302.2 0 70 83 275 1302.2 0 275 84 526 1302.2 0 526 85 151 1302.2 0 151 86 268 1302.2 0 268 87 435 1302.2 0 435 88 273 1302.2 0 273 89 97 1302.2 0 97 90 137 1302.2 0 137 91 144 1302.2 0 144 92 359 1302.2 0 359 93 269 1302.2 0 269 94 151 1302.2 0 151 95 70 1302.2 0 70 96 83 1302.2 0 83 97 49 1302.2 0 49 98 217 1302.2 0 217 99 44 1302.2 0 44 100 25 1302.2 0 25 101 11 1302.2 0 11 102 21 1302.2 0 21 103 20 1302.2 0 20 104 34 1302.2 0 34 105 61 1302.2 0 61 106 15 1302.2 0 15 107 13 1302.2 0 13 108 43 1302.2 0 43 109 29 1302.2 0 29 110 24 1302.2 0 24 111 37 1302.2 0 37 112 29 1302.2 0 29 113 40 1302.2 0 40 114 62 1302.2 0 62 115 46 1302.2 0 46 116 58 1302.2 0 58 117 107 1302.2 0 107 118 104 1302.2 0 104 119 162 1302.2 0 162 120 125 1302.2 0 125 121 283 1302.2 0 283 122 105 1302.2 0 105 123 81 1302.2 0 81 124 129 1302.2 0 129 125 91 1302.2 0 91 126 86 1302.2 0 86 127 69 1302.2 0 69 128 74 1302.2 0 74 129 97 1302.2 0 97 130 289 1302.2 0 289 131 62 1302.2 0 62 132 159 1302.2 0 159 133 125 1302.2 0 125 134 305 1302.2 0 305 135 129 1302.2 0 129 136 84 1302.2 0 84 137 86 1302.2 0 86 138 137 1302.2 0 137 139 132 1302.2 0 132 140 107 1302.2 0 107 141 85 1302.2 0 85 142 77 1302.2 0 77 143 142 1302.2 0 142 144 102 1302.2 0 102 145 126 1302.2 0 126 146 105 1302.2 0 105 147 125 1302.2 0 125 148 123 1302.2 0 123 149 104 1302.2 0 104 150 74 1302.2 0 74 151 89 1302.2 0 89 152 144 1302.2 0 144 153 233 1302.2 0 233 154 291 1302.2 0 291 155 220 1302.2 0 220 156 203 1302.2 0 203 157 119 1302.2 0 119 158 192 1302.2 0 192 159 147 1302.2 0 147 160 286 1302.2 0 286 161 182 1302.2 0 182 162 175 1302.2 0 175 163 277 1302.2 0 277 164 318 1302.2 0 318 165 337 1302.2 0 337 166 214 1302.2 0 214 167 208 1302.2 0 208 168 188 1302.2 0 188 169 120 1302.2 0 120 170 75 1302.2 0 75 171 124 1302.2 0 124 172 348 1302.2 0 348 173 138 1302.2 0 138 174 135 1302.2 0 135 175 139 1302.2 0 139 176 113 1302.2 0 113 177 114 1302.2 0 114 178 207 1302.2 0 207 179 257 1302.2 0 257 180 347 1302.2 0 347 181 336 1302.2 0 336 182 279 1302.2 0 279 183 413 1302.2 0 413 184 535 1302.2 0 535 185 463 1302.2 0 463 186 280 1302.2 0 280 187 66 1302.2 0 66 188 140 1302.2 0 140 189 240 1302.2 0 240 190 250 1302.2 0 250 191 155 1302.2 0 155 192 296 1302.2 0 296 193 206 1302.2 0 206 194 110 1302.2 0 110 195 134 1302.2 0 134 196 321 1302.2 0 321 197 204 1302.2 0 204 198 102 1302.2 0 102 199 121 1302.2 0 121 200 446 1302.2 0 446 201 336 1302.2 0 336 202 261 1302.2 0 261 203 147 1302.2 0 147 204 142 1302.2 0 142 205 225 1302.2 0 225 206 173 1302.2 0 173 207 183 1302.2 0 183 208 282 1302.2 0 282 209 403 1302.2 0 403 210 264 1302.2 0 264 211 150 1302.2 0 150 212 248 1302.2 0 248 213 238 1302.2 0 238 214 205 1302.2 0 205 215 355 1302.2 0 355 216 370 1302.2 0 370 217 460 1302.2 0 460 218 622 1302.2 0 622 219 9428 1302.2 0 9428 220 1191 1302.2 0 1191 221 3270 1302.2 0 3270 222 417 1302.2 0 417 223 214 1302.2 0 214 224 192 1302.2 0 192 225 261 1302.2 0 261 226 378 1302.2 0 378 227 270 1302.2 0 270 228 189 1302.2 0 189 229 169 1302.2 0 169 230 144 1302.2 0 144 231 90 1302.2 0 90 232 223 1302.2 0 223 233 301 1302.2 0 301 234 165 1302.2 0 165 235 94 1302.2 0 94 236 154 1302.2 0 154 237 163 1302.2 0 163 238 131 1302.2 0 131 239 128 1302.2 0 128 240 218 1302.2 0 218 241 151 1302.2 0 151 242 173 1302.2 0 173 243 136 1302.2 0 136 244 163 1302.2 0 163 245 229 1302.2 0 229 246 114 1302.2 0 114 247 118 1302.2 0 118 248 162 1302.2 0 162 249 515 1302.2 0 515 250 1476 1302.2 0 1476 251 737 1302.2 0 737 252 203 1302.2 0 203 253 237 1302.2 0 237 254 532 1302.2 0 532 255 357 1302.2 0 357 256 316 1302.2 0 316 257 259 1302.2 0 259 258 187 1302.2 0 187 259 129 1302.2 0 129 260 111 1302.2 0 111 261 85 1302.2 0 85 262 132 1302.2 0 132 263 195 1302.2 0 195 264 284 1302.2 0 284 265 253 1302.2 0 253 266 161 1302.2 0 161 267 90 1302.2 0 90 268 88 1302.2 0 88 269 198 1302.2 0 198 270 172 1302.2 0 172 271 151 1302.2 0 151 272 136 1302.2 0 136 273 95 1302.2 0 95 274 79 1302.2 0 79 275 91 1302.2 0 91 276 116 1302.2 0 116 277 197 1302.2 0 197 278 200 1302.2 0 200 279 313 1302.2 0 313 280 270 1302.2 0 270 281 298 1302.2 0 298 282 214 1302.2 0 214 283 156 1302.2 0 156 284 203 1302.2 0 203 285 256 1302.2 0 256 286 134 1302.2 0 134 287 103 1302.2 0 103 288 118 1302.2 0 118 289 119 1302.2 0 119 290 193 1302.2 0 193 291 235 1302.2 0 235 292 216 1302.2 0 216 293 124 1302.2 0 124 294 121 1302.2 0 121 295 125 1302.2 0 125 296 96 1302.2 0 96 297 220 1302.2 0 220 298 170 1302.2 0 170 299 61 1302.2 0 61 300 40 1302.2 0 40 301 71 1302.2 0 71 Finished in 2.94 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 54,726 Reads with adapters: 14,958 (27.3%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 14,938 (27.3%) Total basepairs processed: 12,996,997 bp Total written (filtered): 4,092,369 bp (31.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14958 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 17 0.0 3 0 0 14 3 19 229 0.0 3 0 203 10 4 12 20 14633 0.0 4 14223 338 43 20 9 21 34 0.0 4 3 22 0 7 2 22 7 0.0 4 0 0 0 1 6 23 7 0.0 4 0 0 0 7 34 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 53 2 0.0 4 0 0 0 0 2 58 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 146 3 0.0 4 0 0 0 0 3 183 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.64 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 14,938 Reads with adapters: 483 (3.2%) Reads written (passing filters): 14,938 (100.0%) Total basepairs processed: 4,092,369 bp Total written (filtered): 4,084,642 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 483 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.2% C: 4.1% G: 87.8% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 13 233.4 0 13 4 4 58.4 0 4 10 3 0.0 1 0 3 11 424 0.0 1 0 424 12 24 0.0 1 0 24 13 2 0.0 1 0 2 15 2 0.0 1 0 2 225 7 0.0 1 6 1 259 2 0.0 1 2 267 1 0.0 1 1 274 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.