This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 178.96 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 2,932,302 (55.0%) Reads that were too short: 994,616 (18.6%) Reads written (passing filters): 1,937,686 (36.3%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 322,970,836 bp (20.1%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 2932302 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 205 1302.2 0 205 7 661 1302.2 0 661 8 302 1302.2 0 302 9 14150 1302.2 0 14150 10 25 1302.2 0 25 11 3 1302.2 0 3 12 2 1302.2 0 2 13 8 1302.2 0 8 14 7 1302.2 0 7 15 9 1302.2 0 9 16 7 1302.2 0 7 17 13 1302.2 0 13 18 11 1302.2 0 11 19 4 1302.2 0 4 20 4 1302.2 0 4 21 6 1302.2 0 6 22 5 1302.2 0 5 23 7 1302.2 0 7 24 6 1302.2 0 6 25 8 1302.2 0 8 26 4 1302.2 0 4 27 36 1302.2 0 36 28 13 1302.2 0 13 29 6 1302.2 0 6 30 6 1302.2 0 6 31 17 1302.2 0 17 32 14 1302.2 0 14 33 22 1302.2 0 22 34 43 1302.2 0 43 35 97 1302.2 0 97 36 4608 1302.2 0 4608 37 81 1302.2 0 81 38 22 1302.2 0 22 39 4 1302.2 0 4 40 4 1302.2 0 4 41 33 1302.2 0 33 42 59 1302.2 0 59 43 79 1302.2 0 79 44 3 1302.2 0 3 45 7 1302.2 0 7 46 10 1302.2 0 10 47 2 1302.2 0 2 48 7 1302.2 0 7 49 4 1302.2 0 4 50 14 1302.2 0 14 51 16 1302.2 0 16 52 51 1302.2 0 51 53 6 1302.2 0 6 54 2 1302.2 0 2 55 8 1302.2 0 8 56 8 1302.2 0 8 57 5 1302.2 0 5 58 13 1302.2 0 13 59 4 1302.2 0 4 60 7 1302.2 0 7 61 7 1302.2 0 7 62 8 1302.2 0 8 63 10 1302.2 0 10 64 4 1302.2 0 4 65 6 1302.2 0 6 66 4 1302.2 0 4 67 19 1302.2 0 19 68 89 1302.2 0 89 69 93 1302.2 0 93 70 123 1302.2 0 123 71 174 1302.2 0 174 72 224 1302.2 0 224 73 277 1302.2 0 277 74 214 1302.2 0 214 75 474 1302.2 0 474 76 371 1302.2 0 371 77 2278 1302.2 0 2278 78 7363 1302.2 0 7363 79 10558 1302.2 0 10558 80 13833 1302.2 0 13833 81 18929 1302.2 0 18929 82 19427 1302.2 0 19427 83 18441 1302.2 0 18441 84 19268 1302.2 0 19268 85 25885 1302.2 0 25885 86 20131 1302.2 0 20131 87 9198 1302.2 0 9198 88 9017 1302.2 0 9017 89 19486 1302.2 0 19486 90 8446 1302.2 0 8446 91 40674 1302.2 0 40674 92 5116 1302.2 0 5116 93 19487 1302.2 0 19487 94 12435 1302.2 0 12435 95 38231 1302.2 0 38231 96 17663 1302.2 0 17663 97 8352 1302.2 0 8352 98 3219 1302.2 0 3219 99 6289 1302.2 0 6289 100 5290 1302.2 0 5290 101 13343 1302.2 0 13343 102 12453 1302.2 0 12453 103 16627 1302.2 0 16627 104 10667 1302.2 0 10667 105 37489 1302.2 0 37489 106 26711 1302.2 0 26711 107 24024 1302.2 0 24024 108 11956 1302.2 0 11956 109 11730 1302.2 0 11730 110 10933 1302.2 0 10933 111 25477 1302.2 0 25477 112 18789 1302.2 0 18789 113 27788 1302.2 0 27788 114 18554 1302.2 0 18554 115 24014 1302.2 0 24014 116 7990 1302.2 0 7990 117 12631 1302.2 0 12631 118 13152 1302.2 0 13152 119 16846 1302.2 0 16846 120 18677 1302.2 0 18677 121 20084 1302.2 0 20084 122 14778 1302.2 0 14778 123 18359 1302.2 0 18359 124 18953 1302.2 0 18953 125 22493 1302.2 0 22493 126 28599 1302.2 0 28599 127 21722 1302.2 0 21722 128 17128 1302.2 0 17128 129 9604 1302.2 0 9604 130 16487 1302.2 0 16487 131 9225 1302.2 0 9225 132 19289 1302.2 0 19289 133 23213 1302.2 0 23213 134 22110 1302.2 0 22110 135 18735 1302.2 0 18735 136 13270 1302.2 0 13270 137 11674 1302.2 0 11674 138 10844 1302.2 0 10844 139 12325 1302.2 0 12325 140 14893 1302.2 0 14893 141 18582 1302.2 0 18582 142 11086 1302.2 0 11086 143 12379 1302.2 0 12379 144 11780 1302.2 0 11780 145 18258 1302.2 0 18258 146 21881 1302.2 0 21881 147 14584 1302.2 0 14584 148 21288 1302.2 0 21288 149 17545 1302.2 0 17545 150 20937 1302.2 0 20937 151 12578 1302.2 0 12578 152 17575 1302.2 0 17575 153 20880 1302.2 0 20880 154 20228 1302.2 0 20228 155 14785 1302.2 0 14785 156 13894 1302.2 0 13894 157 10725 1302.2 0 10725 158 13701 1302.2 0 13701 159 12391 1302.2 0 12391 160 14923 1302.2 0 14923 161 17219 1302.2 0 17219 162 20544 1302.2 0 20544 163 21931 1302.2 0 21931 164 19186 1302.2 0 19186 165 15845 1302.2 0 15845 166 11638 1302.2 0 11638 167 9737 1302.2 0 9737 168 9463 1302.2 0 9463 169 12507 1302.2 0 12507 170 12666 1302.2 0 12666 171 9830 1302.2 0 9830 172 16570 1302.2 0 16570 173 12272 1302.2 0 12272 174 24916 1302.2 0 24916 175 11553 1302.2 0 11553 176 9643 1302.2 0 9643 177 6646 1302.2 0 6646 178 10828 1302.2 0 10828 179 11967 1302.2 0 11967 180 15099 1302.2 0 15099 181 13775 1302.2 0 13775 182 9618 1302.2 0 9618 183 11127 1302.2 0 11127 184 10174 1302.2 0 10174 185 12869 1302.2 0 12869 186 15316 1302.2 0 15316 187 13285 1302.2 0 13285 188 12153 1302.2 0 12153 189 10189 1302.2 0 10189 190 13818 1302.2 0 13818 191 16283 1302.2 0 16283 192 17204 1302.2 0 17204 193 14890 1302.2 0 14890 194 10336 1302.2 0 10336 195 5119 1302.2 0 5119 196 6226 1302.2 0 6226 197 7180 1302.2 0 7180 198 13056 1302.2 0 13056 199 21270 1302.2 0 21270 200 10441 1302.2 0 10441 201 7790 1302.2 0 7790 202 7457 1302.2 0 7457 203 8478 1302.2 0 8478 204 8698 1302.2 0 8698 205 10638 1302.2 0 10638 206 10706 1302.2 0 10706 207 9119 1302.2 0 9119 208 8392 1302.2 0 8392 209 8049 1302.2 0 8049 210 7913 1302.2 0 7913 211 9632 1302.2 0 9632 212 9646 1302.2 0 9646 213 13656 1302.2 0 13656 214 10200 1302.2 0 10200 215 11793 1302.2 0 11793 216 9881 1302.2 0 9881 217 9533 1302.2 0 9533 218 12616 1302.2 0 12616 219 14039 1302.2 0 14039 220 10723 1302.2 0 10723 221 7211 1302.2 0 7211 222 8537 1302.2 0 8537 223 7819 1302.2 0 7819 224 11722 1302.2 0 11722 225 12701 1302.2 0 12701 226 11553 1302.2 0 11553 227 13173 1302.2 0 13173 228 10574 1302.2 0 10574 229 12368 1302.2 0 12368 230 13267 1302.2 0 13267 231 11682 1302.2 0 11682 232 6868 1302.2 0 6868 233 7072 1302.2 0 7072 234 9850 1302.2 0 9850 235 10383 1302.2 0 10383 236 12255 1302.2 0 12255 237 12410 1302.2 0 12410 238 12182 1302.2 0 12182 239 13627 1302.2 0 13627 240 10282 1302.2 0 10282 241 7942 1302.2 0 7942 242 7589 1302.2 0 7589 243 7320 1302.2 0 7320 244 7411 1302.2 0 7411 245 10195 1302.2 0 10195 246 12205 1302.2 0 12205 247 19036 1302.2 0 19036 248 15071 1302.2 0 15071 249 12073 1302.2 0 12073 250 9719 1302.2 0 9719 251 10819 1302.2 0 10819 252 8710 1302.2 0 8710 253 10369 1302.2 0 10369 254 8915 1302.2 0 8915 255 11533 1302.2 0 11533 256 12747 1302.2 0 12747 257 15731 1302.2 0 15731 258 11074 1302.2 0 11074 259 10862 1302.2 0 10862 260 6522 1302.2 0 6522 261 6229 1302.2 0 6229 262 6096 1302.2 0 6096 263 5840 1302.2 0 5840 264 7664 1302.2 0 7664 265 8247 1302.2 0 8247 266 10516 1302.2 0 10516 267 14604 1302.2 0 14604 268 11514 1302.2 0 11514 269 11732 1302.2 0 11732 270 8324 1302.2 0 8324 271 7814 1302.2 0 7814 272 7610 1302.2 0 7610 273 10142 1302.2 0 10142 274 10750 1302.2 0 10750 275 9669 1302.2 0 9669 276 7859 1302.2 0 7859 277 6989 1302.2 0 6989 278 8067 1302.2 0 8067 279 11981 1302.2 0 11981 280 12354 1302.2 0 12354 281 6872 1302.2 0 6872 282 9407 1302.2 0 9407 283 6091 1302.2 0 6091 284 10655 1302.2 0 10655 285 9852 1302.2 0 9852 286 11628 1302.2 0 11628 287 12337 1302.2 0 12337 288 9175 1302.2 0 9175 289 10081 1302.2 0 10081 290 6857 1302.2 0 6857 291 9887 1302.2 0 9887 292 8466 1302.2 0 8466 293 12127 1302.2 0 12127 294 10536 1302.2 0 10536 295 10740 1302.2 0 10740 296 7055 1302.2 0 7055 297 5834 1302.2 0 5834 298 3599 1302.2 0 3599 299 3870 1302.2 0 3870 300 5820 1302.2 0 5820 301 17178 1302.2 0 17178 Finished in 69.07 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 1,937,686 Reads with adapters: 14,803 (0.8%) Reads that were too short: 154 (0.0%) Reads written (passing filters): 14,649 (0.8%) Total basepairs processed: 322,970,836 bp Total written (filtered): 4,008,892 bp (1.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14803 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 2 0.0 3 0 0 2 16 83 0.0 3 0 74 5 4 17 14221 0.0 3 13851 359 8 3 18 82 0.0 3 5 39 31 7 19 120 0.0 3 8 1 47 64 20 19 0.0 3 0 0 0 19 21 2 0.0 3 0 0 0 2 22 2 0.0 3 0 0 0 2 23 2 0.0 3 0 0 0 2 24 3 0.0 3 0 0 0 3 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 4 0.0 3 0 0 0 4 30 4 0.0 3 0 0 0 4 31 1 0.0 3 0 0 0 1 32 3 0.0 3 0 0 0 3 33 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 37 9 0.0 3 0 0 0 9 38 3 0.0 3 0 0 0 3 39 3 0.0 3 0 0 0 3 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 2 0.0 3 0 0 0 2 50 6 0.0 3 0 0 0 6 52 2 0.0 3 0 0 0 2 53 4 0.0 3 0 0 0 4 55 3 0.0 3 0 0 0 3 57 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 0 1 59 4 0.0 3 0 0 0 4 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 1 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 1 1 66 5 0.0 3 0 0 0 5 67 5 0.0 3 0 0 0 5 68 1 0.0 3 0 0 0 1 69 4 0.0 3 0 0 0 4 70 5 0.0 3 0 0 1 4 71 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 0 2 74 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 82 3 0.0 3 0 0 0 3 83 1 0.0 3 0 0 0 1 84 4 0.0 3 0 0 1 3 86 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 89 7 0.0 3 0 0 0 7 90 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 92 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 0 2 94 4 0.0 3 0 0 1 3 95 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 105 8 0.0 3 0 0 0 8 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 108 4 0.0 3 0 0 0 4 109 2 0.0 3 0 0 0 2 110 3 0.0 3 0 0 0 3 111 4 0.0 3 0 0 0 4 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 1 2 116 2 0.0 3 0 0 0 2 117 3 0.0 3 0 0 0 3 118 1 0.0 3 0 0 0 1 119 3 0.0 3 0 0 0 3 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 3 0.0 3 0 0 0 3 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 3 0.0 3 0 0 0 3 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 3 0.0 3 0 0 0 3 155 3 0.0 3 0 0 0 3 156 5 0.0 3 0 0 0 5 157 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 0 2 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 3 0.0 3 0 0 0 3 171 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 198 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.68 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 14,649 Reads with adapters: 1,723 (11.8%) Reads written (passing filters): 14,649 (100.0%) Total basepairs processed: 4,008,892 bp Total written (filtered): 4,003,721 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1723 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.4% C: 15.2% G: 60.5% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1721 228.9 0 1721 4 2 57.2 0 2