This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 175.52 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 631,114 (11.8%) Reads that were too short: 240,448 (4.5%) Reads written (passing filters): 390,666 (7.3%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 69,361,403 bp (4.3%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 631114 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 115 1302.2 0 115 7 2963 1302.2 0 2963 8 3111 1302.2 0 3111 9 19 1302.2 0 19 10 79 1302.2 0 79 11 91 1302.2 0 91 12 91 1302.2 0 91 13 49 1302.2 0 49 14 140 1302.2 0 140 15 8 1302.2 0 8 16 5 1302.2 0 5 17 1 1302.2 0 1 18 19 1302.2 0 19 19 2 1302.2 0 2 20 12 1302.2 0 12 21 3 1302.2 0 3 22 7 1302.2 0 7 23 21 1302.2 0 21 24 19 1302.2 0 19 25 12 1302.2 0 12 26 11 1302.2 0 11 27 9 1302.2 0 9 28 17 1302.2 0 17 29 15 1302.2 0 15 30 15 1302.2 0 15 31 8 1302.2 0 8 32 24 1302.2 0 24 33 8 1302.2 0 8 34 3 1302.2 0 3 35 17 1302.2 0 17 36 3 1302.2 0 3 37 44 1302.2 0 44 38 19 1302.2 0 19 39 31 1302.2 0 31 40 16 1302.2 0 16 41 9 1302.2 0 9 42 23 1302.2 0 23 43 17 1302.2 0 17 44 15 1302.2 0 15 45 14 1302.2 0 14 46 15 1302.2 0 15 47 18 1302.2 0 18 48 12 1302.2 0 12 49 24 1302.2 0 24 50 12 1302.2 0 12 51 20 1302.2 0 20 52 110 1302.2 0 110 53 21 1302.2 0 21 54 52 1302.2 0 52 55 46 1302.2 0 46 56 15 1302.2 0 15 57 119 1302.2 0 119 58 5 1302.2 0 5 59 31 1302.2 0 31 60 23 1302.2 0 23 61 52 1302.2 0 52 62 212 1302.2 0 212 63 39 1302.2 0 39 64 123 1302.2 0 123 65 67 1302.2 0 67 66 139 1302.2 0 139 67 124 1302.2 0 124 68 45 1302.2 0 45 69 56 1302.2 0 56 70 44 1302.2 0 44 71 88 1302.2 0 88 72 336 1302.2 0 336 73 1236 1302.2 0 1236 74 549 1302.2 0 549 75 1675 1302.2 0 1675 76 1115 1302.2 0 1115 77 3149 1302.2 0 3149 78 4608 1302.2 0 4608 79 55723 1302.2 0 55723 80 6960 1302.2 0 6960 81 40076 1302.2 0 40076 82 2093 1302.2 0 2093 83 2718 1302.2 0 2718 84 4550 1302.2 0 4550 85 3330 1302.2 0 3330 86 4020 1302.2 0 4020 87 4230 1302.2 0 4230 88 2964 1302.2 0 2964 89 1082 1302.2 0 1082 90 873 1302.2 0 873 91 4402 1302.2 0 4402 92 779 1302.2 0 779 93 3293 1302.2 0 3293 94 1844 1302.2 0 1844 95 1240 1302.2 0 1240 96 815 1302.2 0 815 97 933 1302.2 0 933 98 470 1302.2 0 470 99 773 1302.2 0 773 100 520 1302.2 0 520 101 1500 1302.2 0 1500 102 638 1302.2 0 638 103 894 1302.2 0 894 104 401 1302.2 0 401 105 492 1302.2 0 492 106 309 1302.2 0 309 107 350 1302.2 0 350 108 411 1302.2 0 411 109 404 1302.2 0 404 110 506 1302.2 0 506 111 1179 1302.2 0 1179 112 955 1302.2 0 955 113 1069 1302.2 0 1069 114 873 1302.2 0 873 115 1596 1302.2 0 1596 116 1819 1302.2 0 1819 117 6356 1302.2 0 6356 118 2913 1302.2 0 2913 119 3879 1302.2 0 3879 120 2098 1302.2 0 2098 121 1641 1302.2 0 1641 122 2093 1302.2 0 2093 123 1655 1302.2 0 1655 124 1976 1302.2 0 1976 125 2060 1302.2 0 2060 126 1818 1302.2 0 1818 127 1627 1302.2 0 1627 128 1653 1302.2 0 1653 129 1840 1302.2 0 1840 130 3560 1302.2 0 3560 131 2242 1302.2 0 2242 132 4572 1302.2 0 4572 133 1882 1302.2 0 1882 134 2370 1302.2 0 2370 135 1927 1302.2 0 1927 136 1991 1302.2 0 1991 137 2242 1302.2 0 2242 138 2255 1302.2 0 2255 139 2220 1302.2 0 2220 140 1650 1302.2 0 1650 141 2083 1302.2 0 2083 142 2113 1302.2 0 2113 143 1915 1302.2 0 1915 144 1879 1302.2 0 1879 145 2169 1302.2 0 2169 146 3282 1302.2 0 3282 147 2171 1302.2 0 2171 148 2504 1302.2 0 2504 149 1673 1302.2 0 1673 150 2455 1302.2 0 2455 151 2896 1302.2 0 2896 152 3294 1302.2 0 3294 153 2627 1302.2 0 2627 154 2389 1302.2 0 2389 155 2567 1302.2 0 2567 156 3108 1302.2 0 3108 157 2983 1302.2 0 2983 158 2945 1302.2 0 2945 159 2997 1302.2 0 2997 160 2398 1302.2 0 2398 161 2680 1302.2 0 2680 162 2507 1302.2 0 2507 163 2495 1302.2 0 2495 164 2092 1302.2 0 2092 165 2064 1302.2 0 2064 166 2061 1302.2 0 2061 167 2503 1302.2 0 2503 168 2978 1302.2 0 2978 169 2057 1302.2 0 2057 170 3411 1302.2 0 3411 171 2076 1302.2 0 2076 172 3602 1302.2 0 3602 173 1539 1302.2 0 1539 174 2934 1302.2 0 2934 175 1696 1302.2 0 1696 176 2545 1302.2 0 2545 177 2069 1302.2 0 2069 178 2875 1302.2 0 2875 179 3015 1302.2 0 3015 180 2658 1302.2 0 2658 181 2955 1302.2 0 2955 182 2465 1302.2 0 2465 183 2460 1302.2 0 2460 184 2548 1302.2 0 2548 185 2379 1302.2 0 2379 186 2793 1302.2 0 2793 187 2547 1302.2 0 2547 188 3973 1302.2 0 3973 189 2209 1302.2 0 2209 190 2424 1302.2 0 2424 191 2060 1302.2 0 2060 192 2594 1302.2 0 2594 193 1770 1302.2 0 1770 194 2517 1302.2 0 2517 195 2143 1302.2 0 2143 196 2474 1302.2 0 2474 197 2075 1302.2 0 2075 198 3050 1302.2 0 3050 199 3080 1302.2 0 3080 200 2885 1302.2 0 2885 201 2091 1302.2 0 2091 202 2206 1302.2 0 2206 203 1980 1302.2 0 1980 204 2554 1302.2 0 2554 205 2108 1302.2 0 2108 206 2406 1302.2 0 2406 207 2644 1302.2 0 2644 208 2139 1302.2 0 2139 209 1562 1302.2 0 1562 210 2060 1302.2 0 2060 211 2194 1302.2 0 2194 212 2569 1302.2 0 2569 213 3368 1302.2 0 3368 214 3243 1302.2 0 3243 215 3060 1302.2 0 3060 216 3140 1302.2 0 3140 217 2739 1302.2 0 2739 218 2604 1302.2 0 2604 219 2453 1302.2 0 2453 220 2763 1302.2 0 2763 221 3536 1302.2 0 3536 222 3426 1302.2 0 3426 223 2521 1302.2 0 2521 224 2685 1302.2 0 2685 225 2136 1302.2 0 2136 226 1867 1302.2 0 1867 227 1843 1302.2 0 1843 228 2315 1302.2 0 2315 229 1672 1302.2 0 1672 230 1740 1302.2 0 1740 231 1929 1302.2 0 1929 232 2971 1302.2 0 2971 233 2227 1302.2 0 2227 234 2621 1302.2 0 2621 235 2291 1302.2 0 2291 236 2559 1302.2 0 2559 237 1823 1302.2 0 1823 238 2943 1302.2 0 2943 239 2742 1302.2 0 2742 240 3467 1302.2 0 3467 241 3698 1302.2 0 3698 242 3915 1302.2 0 3915 243 3412 1302.2 0 3412 244 2417 1302.2 0 2417 245 2075 1302.2 0 2075 246 1323 1302.2 0 1323 247 1864 1302.2 0 1864 248 2223 1302.2 0 2223 249 3117 1302.2 0 3117 250 3090 1302.2 0 3090 251 4251 1302.2 0 4251 252 3833 1302.2 0 3833 253 2073 1302.2 0 2073 254 1542 1302.2 0 1542 255 1022 1302.2 0 1022 256 929 1302.2 0 929 257 1072 1302.2 0 1072 258 1357 1302.2 0 1357 259 1555 1302.2 0 1555 260 1879 1302.2 0 1879 261 2027 1302.2 0 2027 262 2499 1302.2 0 2499 263 2558 1302.2 0 2558 264 2484 1302.2 0 2484 265 1691 1302.2 0 1691 266 1674 1302.2 0 1674 267 1663 1302.2 0 1663 268 1758 1302.2 0 1758 269 2486 1302.2 0 2486 270 2442 1302.2 0 2442 271 3366 1302.2 0 3366 272 2740 1302.2 0 2740 273 2408 1302.2 0 2408 274 1894 1302.2 0 1894 275 2592 1302.2 0 2592 276 3202 1302.2 0 3202 277 3161 1302.2 0 3161 278 2977 1302.2 0 2977 279 2289 1302.2 0 2289 280 2488 1302.2 0 2488 281 2193 1302.2 0 2193 282 2271 1302.2 0 2271 283 2289 1302.2 0 2289 284 1984 1302.2 0 1984 285 1542 1302.2 0 1542 286 1551 1302.2 0 1551 287 1846 1302.2 0 1846 288 2389 1302.2 0 2389 289 2651 1302.2 0 2651 290 4175 1302.2 0 4175 291 3623 1302.2 0 3623 292 3197 1302.2 0 3197 293 2230 1302.2 0 2230 294 2411 1302.2 0 2411 295 2390 1302.2 0 2390 296 3030 1302.2 0 3030 297 2371 1302.2 0 2371 298 2331 1302.2 0 2331 299 2011 1302.2 0 2011 300 1414 1302.2 0 1414 301 397 1302.2 0 397 Finished in 17.71 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 390,666 Reads with adapters: 4,110 (1.1%) Reads that were too short: 120 (0.0%) Reads written (passing filters): 3,990 (1.0%) Total basepairs processed: 69,361,403 bp Total written (filtered): 1,082,879 bp (1.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4110 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 4 0.0 3 0 0 4 19 39 0.0 3 0 31 4 2 2 20 3055 0.0 4 2949 93 9 3 1 21 20 0.0 4 1 14 1 0 4 22 824 0.0 4 797 18 6 2 1 23 8 0.0 4 0 5 0 2 1 25 3 0.0 4 0 0 0 2 1 28 1 0.0 4 0 0 0 0 1 29 3 0.0 4 0 0 0 0 3 31 2 0.0 4 0 0 0 0 2 33 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 38 2 0.0 4 0 0 0 0 2 40 3 0.0 4 0 0 0 0 3 41 1 0.0 4 0 0 0 0 1 42 2 0.0 4 0 0 0 1 1 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 55 2 0.0 4 0 0 0 0 2 57 3 0.0 4 0 0 0 0 3 58 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 2 0.0 4 0 0 0 0 2 62 4 0.0 4 0 0 0 1 3 63 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 66 2 0.0 4 0 0 0 0 2 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 3 0.0 4 0 0 0 0 3 74 2 0.0 4 0 0 0 0 2 76 3 0.0 4 0 0 0 0 3 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 84 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 86 2 0.0 4 0 0 0 1 1 87 2 0.0 4 0 0 0 0 2 88 4 0.0 4 0 0 0 0 4 89 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 94 3 0.0 4 0 0 0 0 3 95 1 0.0 4 0 0 0 0 1 96 3 0.0 4 0 0 0 0 3 97 3 0.0 4 0 0 0 0 3 99 2 0.0 4 0 0 0 0 2 101 3 0.0 4 0 0 0 0 3 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 110 2 0.0 4 0 0 0 0 2 111 4 0.0 4 0 0 0 0 4 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 115 2 0.0 4 0 0 0 0 2 116 2 0.0 4 0 0 0 0 2 118 3 0.0 4 0 0 0 0 3 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 2 0.0 4 0 0 0 0 2 123 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 136 2 0.0 4 0 0 0 0 2 137 1 0.0 4 0 0 0 0 1 138 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 147 1 0.0 4 0 0 0 0 1 151 2 0.0 4 0 0 0 0 2 152 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 163 3 0.0 4 0 0 0 0 3 164 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 170 2 0.0 4 0 0 0 0 2 172 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.18 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 3,990 Reads with adapters: 709 (17.8%) Reads written (passing filters): 3,990 (100.0%) Total basepairs processed: 1,082,879 bp Total written (filtered): 1,052,948 bp (97.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 709 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 14.1% C: 1.1% G: 83.1% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 6 1 1.0 0 1 9 18 0.0 0 0 18 10 20 0.0 1 0 20 11 580 0.0 1 0 580 257 76 0.0 1 70 6 258 2 0.0 1 2 259 6 0.0 1 6 260 1 0.0 1 1 261 1 0.0 1 1 263 2 0.0 1 1 1 266 1 0.0 1 1 268 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.