This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 170.72 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 183,721 (3.4%) Reads that were too short: 56,310 (1.1%) Reads written (passing filters): 127,411 (2.4%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 27,302,141 bp (1.7%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 183721 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 431 1302.2 0 431 7 6690 1302.2 0 6690 8 368 1302.2 0 368 9 18286 1302.2 0 18286 10 44 1302.2 0 44 11 17 1302.2 0 17 12 8 1302.2 0 8 13 7 1302.2 0 7 14 9 1302.2 0 9 15 7 1302.2 0 7 16 8 1302.2 0 8 17 10 1302.2 0 10 18 11 1302.2 0 11 19 12 1302.2 0 12 20 10 1302.2 0 10 21 9 1302.2 0 9 22 7 1302.2 0 7 23 16 1302.2 0 16 24 21 1302.2 0 21 25 15 1302.2 0 15 26 10 1302.2 0 10 27 47 1302.2 0 47 28 33 1302.2 0 33 29 23 1302.2 0 23 30 142 1302.2 0 142 31 5 1302.2 0 5 32 8 1302.2 0 8 33 11 1302.2 0 11 34 177 1302.2 0 177 35 76 1302.2 0 76 36 31 1302.2 0 31 37 112 1302.2 0 112 38 42 1302.2 0 42 39 47 1302.2 0 47 40 22 1302.2 0 22 41 12 1302.2 0 12 42 28 1302.2 0 28 43 20 1302.2 0 20 44 32 1302.2 0 32 45 16 1302.2 0 16 46 34 1302.2 0 34 47 50 1302.2 0 50 48 91 1302.2 0 91 49 121 1302.2 0 121 50 199 1302.2 0 199 51 237 1302.2 0 237 52 455 1302.2 0 455 53 10139 1302.2 0 10139 54 16918 1302.2 0 16918 55 4820 1302.2 0 4820 56 8063 1302.2 0 8063 57 49 1302.2 0 49 58 11 1302.2 0 11 59 9 1302.2 0 9 60 14 1302.2 0 14 61 4 1302.2 0 4 62 7 1302.2 0 7 63 4 1302.2 0 4 64 9 1302.2 0 9 65 6 1302.2 0 6 66 5 1302.2 0 5 67 5 1302.2 0 5 68 7 1302.2 0 7 69 10 1302.2 0 10 70 4 1302.2 0 4 71 3 1302.2 0 3 72 25 1302.2 0 25 73 19 1302.2 0 19 74 107 1302.2 0 107 75 378 1302.2 0 378 76 45 1302.2 0 45 77 78 1302.2 0 78 78 127 1302.2 0 127 79 96 1302.2 0 96 80 91 1302.2 0 91 81 268 1302.2 0 268 82 213 1302.2 0 213 83 423 1302.2 0 423 84 250 1302.2 0 250 85 211 1302.2 0 211 86 159 1302.2 0 159 87 623 1302.2 0 623 88 1080 1302.2 0 1080 89 83 1302.2 0 83 90 126 1302.2 0 126 91 522 1302.2 0 522 92 319 1302.2 0 319 93 564 1302.2 0 564 94 937 1302.2 0 937 95 1058 1302.2 0 1058 96 1791 1302.2 0 1791 97 362 1302.2 0 362 98 509 1302.2 0 509 99 156 1302.2 0 156 100 73 1302.2 0 73 101 112 1302.2 0 112 102 118 1302.2 0 118 103 112 1302.2 0 112 104 179 1302.2 0 179 105 95 1302.2 0 95 106 16 1302.2 0 16 107 69 1302.2 0 69 108 411 1302.2 0 411 109 308 1302.2 0 308 110 112 1302.2 0 112 111 276 1302.2 0 276 112 119 1302.2 0 119 113 222 1302.2 0 222 114 179 1302.2 0 179 115 136 1302.2 0 136 116 376 1302.2 0 376 117 654 1302.2 0 654 118 295 1302.2 0 295 119 987 1302.2 0 987 120 459 1302.2 0 459 121 1208 1302.2 0 1208 122 912 1302.2 0 912 123 979 1302.2 0 979 124 521 1302.2 0 521 125 259 1302.2 0 259 126 329 1302.2 0 329 127 243 1302.2 0 243 128 371 1302.2 0 371 129 706 1302.2 0 706 130 370 1302.2 0 370 131 275 1302.2 0 275 132 266 1302.2 0 266 133 285 1302.2 0 285 134 325 1302.2 0 325 135 404 1302.2 0 404 136 386 1302.2 0 386 137 414 1302.2 0 414 138 327 1302.2 0 327 139 789 1302.2 0 789 140 480 1302.2 0 480 141 614 1302.2 0 614 142 450 1302.2 0 450 143 558 1302.2 0 558 144 256 1302.2 0 256 145 406 1302.2 0 406 146 446 1302.2 0 446 147 901 1302.2 0 901 148 478 1302.2 0 478 149 346 1302.2 0 346 150 524 1302.2 0 524 151 415 1302.2 0 415 152 456 1302.2 0 456 153 282 1302.2 0 282 154 271 1302.2 0 271 155 570 1302.2 0 570 156 588 1302.2 0 588 157 480 1302.2 0 480 158 479 1302.2 0 479 159 468 1302.2 0 468 160 618 1302.2 0 618 161 656 1302.2 0 656 162 813 1302.2 0 813 163 783 1302.2 0 783 164 412 1302.2 0 412 165 522 1302.2 0 522 166 554 1302.2 0 554 167 995 1302.2 0 995 168 666 1302.2 0 666 169 414 1302.2 0 414 170 294 1302.2 0 294 171 523 1302.2 0 523 172 745 1302.2 0 745 173 262 1302.2 0 262 174 262 1302.2 0 262 175 589 1302.2 0 589 176 1870 1302.2 0 1870 177 1558 1302.2 0 1558 178 1414 1302.2 0 1414 179 229 1302.2 0 229 180 317 1302.2 0 317 181 593 1302.2 0 593 182 626 1302.2 0 626 183 599 1302.2 0 599 184 440 1302.2 0 440 185 342 1302.2 0 342 186 334 1302.2 0 334 187 450 1302.2 0 450 188 831 1302.2 0 831 189 557 1302.2 0 557 190 356 1302.2 0 356 191 232 1302.2 0 232 192 338 1302.2 0 338 193 465 1302.2 0 465 194 468 1302.2 0 468 195 575 1302.2 0 575 196 881 1302.2 0 881 197 337 1302.2 0 337 198 107 1302.2 0 107 199 149 1302.2 0 149 200 459 1302.2 0 459 201 857 1302.2 0 857 202 478 1302.2 0 478 203 229 1302.2 0 229 204 314 1302.2 0 314 205 450 1302.2 0 450 206 298 1302.2 0 298 207 759 1302.2 0 759 208 587 1302.2 0 587 209 413 1302.2 0 413 210 246 1302.2 0 246 211 275 1302.2 0 275 212 655 1302.2 0 655 213 635 1302.2 0 635 214 433 1302.2 0 433 215 562 1302.2 0 562 216 531 1302.2 0 531 217 386 1302.2 0 386 218 325 1302.2 0 325 219 339 1302.2 0 339 220 743 1302.2 0 743 221 1782 1302.2 0 1782 222 760 1302.2 0 760 223 347 1302.2 0 347 224 295 1302.2 0 295 225 496 1302.2 0 496 226 588 1302.2 0 588 227 513 1302.2 0 513 228 465 1302.2 0 465 229 614 1302.2 0 614 230 452 1302.2 0 452 231 361 1302.2 0 361 232 904 1302.2 0 904 233 799 1302.2 0 799 234 314 1302.2 0 314 235 203 1302.2 0 203 236 366 1302.2 0 366 237 539 1302.2 0 539 238 454 1302.2 0 454 239 437 1302.2 0 437 240 466 1302.2 0 466 241 531 1302.2 0 531 242 628 1302.2 0 628 243 394 1302.2 0 394 244 366 1302.2 0 366 245 347 1302.2 0 347 246 319 1302.2 0 319 247 477 1302.2 0 477 248 653 1302.2 0 653 249 605 1302.2 0 605 250 893 1302.2 0 893 251 832 1302.2 0 832 252 566 1302.2 0 566 253 381 1302.2 0 381 254 363 1302.2 0 363 255 441 1302.2 0 441 256 502 1302.2 0 502 257 588 1302.2 0 588 258 726 1302.2 0 726 259 647 1302.2 0 647 260 619 1302.2 0 619 261 444 1302.2 0 444 262 882 1302.2 0 882 263 992 1302.2 0 992 264 727 1302.2 0 727 265 476 1302.2 0 476 266 414 1302.2 0 414 267 363 1302.2 0 363 268 522 1302.2 0 522 269 936 1302.2 0 936 270 706 1302.2 0 706 271 593 1302.2 0 593 272 453 1302.2 0 453 273 395 1302.2 0 395 274 354 1302.2 0 354 275 502 1302.2 0 502 276 707 1302.2 0 707 277 580 1302.2 0 580 278 379 1302.2 0 379 279 335 1302.2 0 335 280 819 1302.2 0 819 281 840 1302.2 0 840 282 1213 1302.2 0 1213 283 807 1302.2 0 807 284 696 1302.2 0 696 285 295 1302.2 0 295 286 440 1302.2 0 440 287 744 1302.2 0 744 288 941 1302.2 0 941 289 1046 1302.2 0 1046 290 676 1302.2 0 676 291 724 1302.2 0 724 292 622 1302.2 0 622 293 470 1302.2 0 470 294 735 1302.2 0 735 295 816 1302.2 0 816 296 637 1302.2 0 637 297 344 1302.2 0 344 298 875 1302.2 0 875 299 658 1302.2 0 658 300 293 1302.2 0 293 301 168 1302.2 0 168 Finished in 6.64 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 127,411 Reads with adapters: 7,018 (5.5%) Reads that were too short: 40 (0.0%) Reads written (passing filters): 6,978 (5.5%) Total basepairs processed: 27,302,141 bp Total written (filtered): 1,890,981 bp (6.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7018 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 60 0.0 3 0 54 1 1 4 20 6708 0.0 4 6547 135 13 5 8 21 13 0.0 4 1 7 0 2 3 22 5 0.0 4 0 0 2 0 3 23 7 0.0 4 0 0 0 7 31 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 47 2 0.0 4 0 0 0 0 2 48 2 0.0 4 0 0 0 1 1 53 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 76 2 0.0 4 0 0 0 0 2 77 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 1 1 109 5 0.0 4 0 0 0 0 5 119 2 0.0 4 0 0 0 0 2 121 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 127 80 0.0 4 0 0 0 0 80 129 1 0.0 4 0 0 0 0 1 135 85 0.0 4 0 0 0 0 85 136 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 163 2 0.0 4 0 0 0 0 2 167 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.29 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 6,978 Reads with adapters: 329 (4.7%) Reads written (passing filters): 6,978 (100.0%) Total basepairs processed: 1,890,981 bp Total written (filtered): 1,863,500 bp (98.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 329 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 31.6% C: 2.4% G: 62.9% T: 2.4% none/other: 0.6% Overview of removed sequences length count expect max.err error counts 3 3 109.0 0 3 4 1 27.3 0 1 10 2 0.0 1 0 2 11 214 0.0 1 0 214 12 12 0.0 1 1 11 16 1 0.0 1 0 1 124 1 0.0 1 1 210 1 0.0 1 1 261 86 0.0 1 80 6 262 2 0.0 1 2 267 1 0.0 1 1 269 1 0.0 1 1 273 2 0.0 1 2 274 2 0.0 1 2