This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 175.89 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 662,041 (12.4%) Reads that were too short: 325,601 (6.1%) Reads written (passing filters): 336,440 (6.3%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 54,138,051 bp (3.4%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 662041 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 40 1302.2 0 40 7 98 1302.2 0 98 8 1431 1302.2 0 1431 9 15 1302.2 0 15 10 20 1302.2 0 20 11 22 1302.2 0 22 12 14 1302.2 0 14 13 10 1302.2 0 10 14 6 1302.2 0 6 15 17 1302.2 0 17 16 28 1302.2 0 28 17 16 1302.2 0 16 18 17 1302.2 0 17 19 17 1302.2 0 17 20 14 1302.2 0 14 21 13 1302.2 0 13 22 15 1302.2 0 15 23 16 1302.2 0 16 24 31 1302.2 0 31 25 47 1302.2 0 47 26 786 1302.2 0 786 27 540 1302.2 0 540 28 581 1302.2 0 581 29 15 1302.2 0 15 30 5 1302.2 0 5 31 14 1302.2 0 14 32 7 1302.2 0 7 33 5 1302.2 0 5 34 16 1302.2 0 16 35 15 1302.2 0 15 36 28 1302.2 0 28 37 28 1302.2 0 28 38 30 1302.2 0 30 39 18 1302.2 0 18 40 63 1302.2 0 63 41 41 1302.2 0 41 42 54 1302.2 0 54 43 30 1302.2 0 30 44 15 1302.2 0 15 45 12 1302.2 0 12 46 13 1302.2 0 13 47 12 1302.2 0 12 48 15 1302.2 0 15 49 19 1302.2 0 19 50 30 1302.2 0 30 51 11 1302.2 0 11 52 32 1302.2 0 32 53 9 1302.2 0 9 54 20 1302.2 0 20 55 8 1302.2 0 8 56 7 1302.2 0 7 57 12 1302.2 0 12 58 141 1302.2 0 141 59 148 1302.2 0 148 60 169 1302.2 0 169 61 19 1302.2 0 19 62 12 1302.2 0 12 63 41 1302.2 0 41 64 19 1302.2 0 19 65 17 1302.2 0 17 66 26 1302.2 0 26 67 17 1302.2 0 17 68 21 1302.2 0 21 69 31 1302.2 0 31 70 27 1302.2 0 27 71 217 1302.2 0 217 72 11 1302.2 0 11 73 392 1302.2 0 392 74 124 1302.2 0 124 75 925 1302.2 0 925 76 355 1302.2 0 355 77 475 1302.2 0 475 78 462 1302.2 0 462 79 1415 1302.2 0 1415 80 620 1302.2 0 620 81 1850 1302.2 0 1850 82 1103 1302.2 0 1103 83 1391 1302.2 0 1391 84 831 1302.2 0 831 85 1034 1302.2 0 1034 86 2334 1302.2 0 2334 87 255 1302.2 0 255 88 1504 1302.2 0 1504 89 1335 1302.2 0 1335 90 426 1302.2 0 426 91 1142 1302.2 0 1142 92 1476 1302.2 0 1476 93 769 1302.2 0 769 94 2092 1302.2 0 2092 95 3181 1302.2 0 3181 96 5258 1302.2 0 5258 97 3989 1302.2 0 3989 98 3612 1302.2 0 3612 99 3317 1302.2 0 3317 100 1711 1302.2 0 1711 101 1551 1302.2 0 1551 102 2830 1302.2 0 2830 103 1658 1302.2 0 1658 104 656 1302.2 0 656 105 2801 1302.2 0 2801 106 3825 1302.2 0 3825 107 2067 1302.2 0 2067 108 9016 1302.2 0 9016 109 5672 1302.2 0 5672 110 3021 1302.2 0 3021 111 1506 1302.2 0 1506 112 4236 1302.2 0 4236 113 3141 1302.2 0 3141 114 10313 1302.2 0 10313 115 6303 1302.2 0 6303 116 2592 1302.2 0 2592 117 2243 1302.2 0 2243 118 1190 1302.2 0 1190 119 806 1302.2 0 806 120 899 1302.2 0 899 121 1399 1302.2 0 1399 122 3480 1302.2 0 3480 123 2191 1302.2 0 2191 124 3565 1302.2 0 3565 125 1300 1302.2 0 1300 126 2097 1302.2 0 2097 127 2619 1302.2 0 2619 128 2207 1302.2 0 2207 129 4446 1302.2 0 4446 130 3704 1302.2 0 3704 131 3678 1302.2 0 3678 132 3100 1302.2 0 3100 133 1949 1302.2 0 1949 134 1935 1302.2 0 1935 135 2343 1302.2 0 2343 136 3546 1302.2 0 3546 137 4810 1302.2 0 4810 138 2873 1302.2 0 2873 139 2421 1302.2 0 2421 140 2283 1302.2 0 2283 141 4511 1302.2 0 4511 142 3493 1302.2 0 3493 143 2344 1302.2 0 2344 144 1442 1302.2 0 1442 145 1456 1302.2 0 1456 146 2135 1302.2 0 2135 147 2825 1302.2 0 2825 148 6309 1302.2 0 6309 149 3205 1302.2 0 3205 150 3898 1302.2 0 3898 151 1764 1302.2 0 1764 152 2036 1302.2 0 2036 153 2636 1302.2 0 2636 154 1902 1302.2 0 1902 155 3187 1302.2 0 3187 156 3144 1302.2 0 3144 157 3338 1302.2 0 3338 158 2132 1302.2 0 2132 159 1975 1302.2 0 1975 160 1959 1302.2 0 1959 161 2130 1302.2 0 2130 162 1932 1302.2 0 1932 163 3426 1302.2 0 3426 164 2616 1302.2 0 2616 165 3202 1302.2 0 3202 166 2346 1302.2 0 2346 167 2620 1302.2 0 2620 168 2391 1302.2 0 2391 169 3133 1302.2 0 3133 170 2299 1302.2 0 2299 171 1142 1302.2 0 1142 172 2053 1302.2 0 2053 173 1247 1302.2 0 1247 174 2612 1302.2 0 2612 175 3384 1302.2 0 3384 176 4685 1302.2 0 4685 177 1959 1302.2 0 1959 178 1492 1302.2 0 1492 179 1318 1302.2 0 1318 180 1387 1302.2 0 1387 181 2049 1302.2 0 2049 182 2387 1302.2 0 2387 183 2374 1302.2 0 2374 184 2076 1302.2 0 2076 185 2977 1302.2 0 2977 186 4418 1302.2 0 4418 187 4427 1302.2 0 4427 188 2833 1302.2 0 2833 189 1481 1302.2 0 1481 190 1374 1302.2 0 1374 191 1609 1302.2 0 1609 192 2771 1302.2 0 2771 193 4418 1302.2 0 4418 194 6897 1302.2 0 6897 195 2350 1302.2 0 2350 196 2432 1302.2 0 2432 197 1534 1302.2 0 1534 198 1566 1302.2 0 1566 199 3559 1302.2 0 3559 200 4816 1302.2 0 4816 201 4083 1302.2 0 4083 202 4197 1302.2 0 4197 203 1402 1302.2 0 1402 204 1660 1302.2 0 1660 205 3060 1302.2 0 3060 206 3838 1302.2 0 3838 207 2449 1302.2 0 2449 208 3679 1302.2 0 3679 209 3088 1302.2 0 3088 210 2796 1302.2 0 2796 211 2513 1302.2 0 2513 212 2694 1302.2 0 2694 213 2405 1302.2 0 2405 214 1965 1302.2 0 1965 215 1655 1302.2 0 1655 216 1988 1302.2 0 1988 217 1757 1302.2 0 1757 218 2338 1302.2 0 2338 219 3657 1302.2 0 3657 220 3096 1302.2 0 3096 221 2581 1302.2 0 2581 222 2583 1302.2 0 2583 223 1743 1302.2 0 1743 224 1321 1302.2 0 1321 225 1728 1302.2 0 1728 226 2319 1302.2 0 2319 227 2978 1302.2 0 2978 228 3634 1302.2 0 3634 229 3784 1302.2 0 3784 230 4960 1302.2 0 4960 231 3179 1302.2 0 3179 232 2047 1302.2 0 2047 233 1913 1302.2 0 1913 234 1919 1302.2 0 1919 235 2453 1302.2 0 2453 236 4124 1302.2 0 4124 237 4884 1302.2 0 4884 238 4529 1302.2 0 4529 239 2975 1302.2 0 2975 240 2753 1302.2 0 2753 241 1862 1302.2 0 1862 242 1801 1302.2 0 1801 243 1718 1302.2 0 1718 244 2185 1302.2 0 2185 245 3176 1302.2 0 3176 246 4464 1302.2 0 4464 247 6348 1302.2 0 6348 248 6575 1302.2 0 6575 249 4447 1302.2 0 4447 250 1977 1302.2 0 1977 251 1048 1302.2 0 1048 252 670 1302.2 0 670 253 851 1302.2 0 851 254 1347 1302.2 0 1347 255 1664 1302.2 0 1664 256 3415 1302.2 0 3415 257 5734 1302.2 0 5734 258 7188 1302.2 0 7188 259 6637 1302.2 0 6637 260 4669 1302.2 0 4669 261 2731 1302.2 0 2731 262 1609 1302.2 0 1609 263 1313 1302.2 0 1313 264 1184 1302.2 0 1184 265 1270 1302.2 0 1270 266 2311 1302.2 0 2311 267 3626 1302.2 0 3626 268 5689 1302.2 0 5689 269 7362 1302.2 0 7362 270 4014 1302.2 0 4014 271 3283 1302.2 0 3283 272 2819 1302.2 0 2819 273 3837 1302.2 0 3837 274 3789 1302.2 0 3789 275 3718 1302.2 0 3718 276 2942 1302.2 0 2942 277 1596 1302.2 0 1596 278 1193 1302.2 0 1193 279 1900 1302.2 0 1900 280 3418 1302.2 0 3418 281 5401 1302.2 0 5401 282 5181 1302.2 0 5181 283 2952 1302.2 0 2952 284 2224 1302.2 0 2224 285 2561 1302.2 0 2561 286 2375 1302.2 0 2375 287 4741 1302.2 0 4741 288 4196 1302.2 0 4196 289 5271 1302.2 0 5271 290 2749 1302.2 0 2749 291 2215 1302.2 0 2215 292 2397 1302.2 0 2397 293 3328 1302.2 0 3328 294 4309 1302.2 0 4309 295 5881 1302.2 0 5881 296 5255 1302.2 0 5255 297 3540 1302.2 0 3540 298 2814 1302.2 0 2814 299 5163 1302.2 0 5163 300 5238 1302.2 0 5238 301 11786 1302.2 0 11786 Finished in 14.26 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 336,440 Reads with adapters: 1,608 (0.5%) Reads that were too short: 139 (0.0%) Reads written (passing filters): 1,469 (0.4%) Total basepairs processed: 54,138,051 bp Total written (filtered): 390,494 bp (0.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1608 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 18 0.0 3 0 13 0 1 4 20 1364 0.0 4 1317 37 4 1 5 21 5 0.0 4 0 4 0 1 22 2 0.0 4 0 0 0 1 1 23 6 0.0 4 0 0 0 2 4 24 4 0.0 4 0 0 0 0 4 25 3 0.0 4 1 0 0 0 2 26 17 0.0 4 0 0 0 0 17 27 2 0.0 4 0 0 0 0 2 29 2 0.0 4 0 0 0 0 2 30 1 0.0 4 0 0 0 0 1 32 3 0.0 4 0 0 0 0 3 33 1 0.0 4 0 0 0 0 1 34 3 0.0 4 0 0 0 0 3 35 4 0.0 4 0 0 0 0 4 36 2 0.0 4 0 0 0 0 2 37 2 0.0 4 0 0 0 0 2 38 2 0.0 4 0 0 0 0 2 41 2 0.0 4 0 0 0 0 2 42 3 0.0 4 0 0 0 0 3 43 2 0.0 4 0 0 0 0 2 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 46 3 0.0 4 0 0 0 0 3 49 3 0.0 4 0 0 0 0 3 51 2 0.0 4 0 0 0 0 2 52 3 0.0 4 0 0 0 0 3 53 3 0.0 4 0 0 0 0 3 54 3 0.0 4 0 0 0 0 3 55 1 0.0 4 0 0 0 1 56 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 59 2 0.0 4 0 0 0 0 2 60 3 0.0 4 0 0 0 0 3 61 4 0.0 4 0 0 0 0 4 62 1 0.0 4 0 0 0 0 1 64 3 0.0 4 0 0 0 0 3 65 3 0.0 4 0 0 0 0 3 67 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 2 0.0 4 0 0 0 0 2 76 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 0 2 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 1 82 2 0.0 4 0 0 0 0 2 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 85 2 0.0 4 0 0 0 0 2 86 4 0.0 4 0 0 0 0 4 87 2 0.0 4 0 0 0 0 2 88 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 3 0.0 4 0 0 0 0 3 93 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 3 0.0 4 0 0 0 0 3 99 2 0.0 4 0 0 0 0 2 101 2 0.0 4 0 0 0 1 1 105 2 0.0 4 0 0 0 0 2 106 1 0.0 4 0 0 0 0 1 107 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 114 4 0.0 4 0 0 0 0 4 116 3 0.0 4 0 0 0 0 3 118 3 0.0 4 0 0 0 0 3 119 2 0.0 4 0 0 0 0 2 120 1 0.0 4 0 0 0 0 1 121 2 0.0 4 0 0 0 0 2 122 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 131 2 0.0 4 0 0 0 0 2 132 2 0.0 4 0 0 0 0 2 133 2 0.0 4 0 0 0 0 2 134 3 0.0 4 0 0 0 1 2 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 142 3 0.0 4 0 0 0 1 2 147 3 0.0 4 0 0 0 0 3 152 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 157 3 0.0 4 0 0 0 1 2 158 1 0.0 4 0 0 0 1 159 2 0.0 4 0 0 0 0 2 161 1 0.0 4 0 0 0 0 1 165 2 0.0 4 0 0 0 0 2 166 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 186 2 0.0 4 0 0 0 0 2 188 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 1,469 Reads with adapters: 283 (19.3%) Reads written (passing filters): 1,469 (100.0%) Total basepairs processed: 390,494 bp Total written (filtered): 387,176 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 283 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 3.2% G: 93.3% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 6 1 0.4 0 1 10 4 0.0 1 0 4 11 276 0.0 1 1 275 12 1 0.0 1 0 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.