This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 179.76 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 962,551 (18.0%) Reads that were too short: 459,291 (8.6%) Reads written (passing filters): 503,260 (9.4%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 81,625,157 bp (5.1%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 962551 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 118 1302.2 0 118 7 1808 1302.2 0 1808 8 2956 1302.2 0 2956 9 125 1302.2 0 125 10 359 1302.2 0 359 11 14067 1302.2 0 14067 12 35 1302.2 0 35 13 18 1302.2 0 18 14 17 1302.2 0 17 15 17 1302.2 0 17 16 26 1302.2 0 26 17 19 1302.2 0 19 18 18 1302.2 0 18 19 16 1302.2 0 16 20 25 1302.2 0 25 21 19 1302.2 0 19 22 26 1302.2 0 26 23 17 1302.2 0 17 24 23 1302.2 0 23 25 27 1302.2 0 27 26 22 1302.2 0 22 27 14 1302.2 0 14 28 11 1302.2 0 11 29 20 1302.2 0 20 30 26 1302.2 0 26 31 22 1302.2 0 22 32 20 1302.2 0 20 33 15 1302.2 0 15 34 25 1302.2 0 25 35 24 1302.2 0 24 36 28 1302.2 0 28 37 15 1302.2 0 15 38 30 1302.2 0 30 39 25 1302.2 0 25 40 17 1302.2 0 17 41 21 1302.2 0 21 42 20 1302.2 0 20 43 23 1302.2 0 23 44 16 1302.2 0 16 45 21 1302.2 0 21 46 15 1302.2 0 15 47 16 1302.2 0 16 48 18 1302.2 0 18 49 18 1302.2 0 18 50 23 1302.2 0 23 51 18 1302.2 0 18 52 30 1302.2 0 30 53 29 1302.2 0 29 54 36 1302.2 0 36 55 21 1302.2 0 21 56 18 1302.2 0 18 57 16 1302.2 0 16 58 20 1302.2 0 20 59 18 1302.2 0 18 60 22 1302.2 0 22 61 18 1302.2 0 18 62 14 1302.2 0 14 63 28 1302.2 0 28 64 21 1302.2 0 21 65 16 1302.2 0 16 66 19 1302.2 0 19 67 15 1302.2 0 15 68 30 1302.2 0 30 69 27 1302.2 0 27 70 30 1302.2 0 30 71 41 1302.2 0 41 72 74 1302.2 0 74 73 45 1302.2 0 45 74 268 1302.2 0 268 75 153 1302.2 0 153 76 119 1302.2 0 119 77 548 1302.2 0 548 78 1990 1302.2 0 1990 79 2253 1302.2 0 2253 80 4609 1302.2 0 4609 81 2944 1302.2 0 2944 82 2134 1302.2 0 2134 83 3114 1302.2 0 3114 84 7554 1302.2 0 7554 85 1618 1302.2 0 1618 86 2232 1302.2 0 2232 87 5878 1302.2 0 5878 88 9990 1302.2 0 9990 89 497 1302.2 0 497 90 951 1302.2 0 951 91 10081 1302.2 0 10081 92 3204 1302.2 0 3204 93 7061 1302.2 0 7061 94 1713 1302.2 0 1713 95 2252 1302.2 0 2252 96 744 1302.2 0 744 97 1244 1302.2 0 1244 98 1867 1302.2 0 1867 99 1411 1302.2 0 1411 100 985 1302.2 0 985 101 3105 1302.2 0 3105 102 1193 1302.2 0 1193 103 2491 1302.2 0 2491 104 8356 1302.2 0 8356 105 3100 1302.2 0 3100 106 1203 1302.2 0 1203 107 2584 1302.2 0 2584 108 2276 1302.2 0 2276 109 1666 1302.2 0 1666 110 1480 1302.2 0 1480 111 2001 1302.2 0 2001 112 1292 1302.2 0 1292 113 2296 1302.2 0 2296 114 1540 1302.2 0 1540 115 1704 1302.2 0 1704 116 2529 1302.2 0 2529 117 6553 1302.2 0 6553 118 1814 1302.2 0 1814 119 3478 1302.2 0 3478 120 3358 1302.2 0 3358 121 3305 1302.2 0 3305 122 3795 1302.2 0 3795 123 5347 1302.2 0 5347 124 5680 1302.2 0 5680 125 4521 1302.2 0 4521 126 3982 1302.2 0 3982 127 1660 1302.2 0 1660 128 2136 1302.2 0 2136 129 3645 1302.2 0 3645 130 6671 1302.2 0 6671 131 3900 1302.2 0 3900 132 7936 1302.2 0 7936 133 3951 1302.2 0 3951 134 4236 1302.2 0 4236 135 3360 1302.2 0 3360 136 3313 1302.2 0 3313 137 3822 1302.2 0 3822 138 3005 1302.2 0 3005 139 3247 1302.2 0 3247 140 1429 1302.2 0 1429 141 2724 1302.2 0 2724 142 4376 1302.2 0 4376 143 7634 1302.2 0 7634 144 2701 1302.2 0 2701 145 3476 1302.2 0 3476 146 3318 1302.2 0 3318 147 3111 1302.2 0 3111 148 3512 1302.2 0 3512 149 3435 1302.2 0 3435 150 4733 1302.2 0 4733 151 4240 1302.2 0 4240 152 5215 1302.2 0 5215 153 3484 1302.2 0 3484 154 3900 1302.2 0 3900 155 6109 1302.2 0 6109 156 4970 1302.2 0 4970 157 5212 1302.2 0 5212 158 6623 1302.2 0 6623 159 3527 1302.2 0 3527 160 3995 1302.2 0 3995 161 3680 1302.2 0 3680 162 4857 1302.2 0 4857 163 5316 1302.2 0 5316 164 3935 1302.2 0 3935 165 3279 1302.2 0 3279 166 3261 1302.2 0 3261 167 4346 1302.2 0 4346 168 3833 1302.2 0 3833 169 3703 1302.2 0 3703 170 7333 1302.2 0 7333 171 10668 1302.2 0 10668 172 6421 1302.2 0 6421 173 2507 1302.2 0 2507 174 3432 1302.2 0 3432 175 2718 1302.2 0 2718 176 3737 1302.2 0 3737 177 3993 1302.2 0 3993 178 7176 1302.2 0 7176 179 6832 1302.2 0 6832 180 5093 1302.2 0 5093 181 3491 1302.2 0 3491 182 4113 1302.2 0 4113 183 4984 1302.2 0 4984 184 3453 1302.2 0 3453 185 2402 1302.2 0 2402 186 1814 1302.2 0 1814 187 3892 1302.2 0 3892 188 7917 1302.2 0 7917 189 8244 1302.2 0 8244 190 5414 1302.2 0 5414 191 3826 1302.2 0 3826 192 3806 1302.2 0 3806 193 2613 1302.2 0 2613 194 3278 1302.2 0 3278 195 10314 1302.2 0 10314 196 4785 1302.2 0 4785 197 1617 1302.2 0 1617 198 2751 1302.2 0 2751 199 2391 1302.2 0 2391 200 3559 1302.2 0 3559 201 4960 1302.2 0 4960 202 3098 1302.2 0 3098 203 2848 1302.2 0 2848 204 3187 1302.2 0 3187 205 2755 1302.2 0 2755 206 1603 1302.2 0 1603 207 2109 1302.2 0 2109 208 2747 1302.2 0 2747 209 2609 1302.2 0 2609 210 2536 1302.2 0 2536 211 2783 1302.2 0 2783 212 2651 1302.2 0 2651 213 3177 1302.2 0 3177 214 5094 1302.2 0 5094 215 5807 1302.2 0 5807 216 5816 1302.2 0 5816 217 5385 1302.2 0 5385 218 3788 1302.2 0 3788 219 2121 1302.2 0 2121 220 1972 1302.2 0 1972 221 5707 1302.2 0 5707 222 5360 1302.2 0 5360 223 4043 1302.2 0 4043 224 5255 1302.2 0 5255 225 5869 1302.2 0 5869 226 8938 1302.2 0 8938 227 13354 1302.2 0 13354 228 8310 1302.2 0 8310 229 8344 1302.2 0 8344 230 5511 1302.2 0 5511 231 6136 1302.2 0 6136 232 9601 1302.2 0 9601 233 8802 1302.2 0 8802 234 2738 1302.2 0 2738 235 2249 1302.2 0 2249 236 2842 1302.2 0 2842 237 2776 1302.2 0 2776 238 2514 1302.2 0 2514 239 3104 1302.2 0 3104 240 2856 1302.2 0 2856 241 3522 1302.2 0 3522 242 4301 1302.2 0 4301 243 3469 1302.2 0 3469 244 3383 1302.2 0 3383 245 2652 1302.2 0 2652 246 1826 1302.2 0 1826 247 2219 1302.2 0 2219 248 5707 1302.2 0 5707 249 12494 1302.2 0 12494 250 11928 1302.2 0 11928 251 6001 1302.2 0 6001 252 5504 1302.2 0 5504 253 7362 1302.2 0 7362 254 4737 1302.2 0 4737 255 3800 1302.2 0 3800 256 2373 1302.2 0 2373 257 3074 1302.2 0 3074 258 2913 1302.2 0 2913 259 4148 1302.2 0 4148 260 3922 1302.2 0 3922 261 5333 1302.2 0 5333 262 4359 1302.2 0 4359 263 5229 1302.2 0 5229 264 6902 1302.2 0 6902 265 5113 1302.2 0 5113 266 3349 1302.2 0 3349 267 2036 1302.2 0 2036 268 2601 1302.2 0 2601 269 3828 1302.2 0 3828 270 3776 1302.2 0 3776 271 5886 1302.2 0 5886 272 5981 1302.2 0 5981 273 6038 1302.2 0 6038 274 7354 1302.2 0 7354 275 6714 1302.2 0 6714 276 8005 1302.2 0 8005 277 6396 1302.2 0 6396 278 4437 1302.2 0 4437 279 3348 1302.2 0 3348 280 4055 1302.2 0 4055 281 7918 1302.2 0 7918 282 6590 1302.2 0 6590 283 3712 1302.2 0 3712 284 5745 1302.2 0 5745 285 3760 1302.2 0 3760 286 2362 1302.2 0 2362 287 1829 1302.2 0 1829 288 1939 1302.2 0 1939 289 3729 1302.2 0 3729 290 4564 1302.2 0 4564 291 7426 1302.2 0 7426 292 8721 1302.2 0 8721 293 7458 1302.2 0 7458 294 1406 1302.2 0 1406 295 1459 1302.2 0 1459 296 1647 1302.2 0 1647 297 3325 1302.2 0 3325 298 4367 1302.2 0 4367 299 4061 1302.2 0 4061 300 2967 1302.2 0 2967 301 1836 1302.2 0 1836 Finished in 18.33 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 503,260 Reads with adapters: 1,683 (0.3%) Reads that were too short: 80 (0.0%) Reads written (passing filters): 1,603 (0.3%) Total basepairs processed: 81,625,157 bp Total written (filtered): 432,961 bp (0.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1683 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 11 0.0 3 0 10 1 17 1509 0.0 3 1478 30 1 18 3 0.0 3 0 2 1 19 6 0.0 3 0 0 2 4 20 4 0.0 3 0 0 0 4 22 3 0.0 3 0 0 0 3 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 1 1 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 5 0.0 3 0 0 0 5 69 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 4 0.0 3 0 0 0 4 89 6 0.0 3 0 0 0 6 90 2 0.0 3 0 0 0 2 91 4 0.0 3 0 0 0 4 92 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 3 0.0 3 0 0 0 3 108 3 0.0 3 0 0 0 3 109 1 0.0 3 0 0 0 1 110 3 0.0 3 0 0 0 3 114 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 1 134 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 165 3 0.0 3 0 0 0 3 167 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 182 3 0.0 3 0 0 0 3 183 5 0.0 3 0 0 0 5 184 1 0.0 3 0 0 0 1 192 5 0.0 3 0 0 0 5 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 217 6 0.0 3 0 0 0 6 220 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 277 2 0.0 3 0 0 0 2 278 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 284 4 0.0 3 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 1,603 Reads with adapters: 128 (8.0%) Reads written (passing filters): 1,603 (100.0%) Total basepairs processed: 432,961 bp Total written (filtered): 432,577 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 128 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 13.3% C: 75.0% G: 10.2% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 128 25.0 0 128