This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 170.78 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 175,004 (3.3%) Reads that were too short: 70,367 (1.3%) Reads written (passing filters): 104,637 (2.0%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 23,991,490 bp (1.5%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 175004 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2496 1302.2 0 2496 7 37248 1302.2 0 37248 8 310 1302.2 0 310 9 16489 1302.2 0 16489 10 15 1302.2 0 15 17 2 1302.2 0 2 18 3 1302.2 0 3 19 1 1302.2 0 1 20 1 1302.2 0 1 21 1 1302.2 0 1 22 1 1302.2 0 1 24 3 1302.2 0 3 26 3 1302.2 0 3 27 3 1302.2 0 3 28 1 1302.2 0 1 29 1 1302.2 0 1 30 4 1302.2 0 4 31 1 1302.2 0 1 33 1 1302.2 0 1 34 1 1302.2 0 1 35 6 1302.2 0 6 36 6 1302.2 0 6 37 1 1302.2 0 1 38 1 1302.2 0 1 39 4 1302.2 0 4 40 5 1302.2 0 5 41 7 1302.2 0 7 42 3 1302.2 0 3 43 8 1302.2 0 8 44 9 1302.2 0 9 45 2 1302.2 0 2 46 11 1302.2 0 11 47 2 1302.2 0 2 48 3 1302.2 0 3 49 1 1302.2 0 1 50 13 1302.2 0 13 51 1 1302.2 0 1 52 2 1302.2 0 2 53 1 1302.2 0 1 54 4 1302.2 0 4 55 2 1302.2 0 2 57 2 1302.2 0 2 58 3 1302.2 0 3 59 2 1302.2 0 2 60 2 1302.2 0 2 61 1 1302.2 0 1 62 1 1302.2 0 1 63 4 1302.2 0 4 64 1 1302.2 0 1 66 34 1302.2 0 34 67 31 1302.2 0 31 68 54 1302.2 0 54 69 87 1302.2 0 87 70 21 1302.2 0 21 71 1 1302.2 0 1 72 3 1302.2 0 3 73 4 1302.2 0 4 74 6 1302.2 0 6 75 6 1302.2 0 6 78 12 1302.2 0 12 79 93 1302.2 0 93 80 41 1302.2 0 41 81 124 1302.2 0 124 82 217 1302.2 0 217 83 97 1302.2 0 97 84 73 1302.2 0 73 85 56 1302.2 0 56 86 60 1302.2 0 60 87 34 1302.2 0 34 88 136 1302.2 0 136 89 16 1302.2 0 16 90 69 1302.2 0 69 91 19 1302.2 0 19 92 35 1302.2 0 35 93 8 1302.2 0 8 94 51 1302.2 0 51 95 317 1302.2 0 317 96 730 1302.2 0 730 97 447 1302.2 0 447 98 535 1302.2 0 535 99 393 1302.2 0 393 100 299 1302.2 0 299 101 1248 1302.2 0 1248 102 939 1302.2 0 939 103 252 1302.2 0 252 104 199 1302.2 0 199 105 93 1302.2 0 93 106 45 1302.2 0 45 107 146 1302.2 0 146 108 261 1302.2 0 261 109 124 1302.2 0 124 110 113 1302.2 0 113 111 586 1302.2 0 586 112 1001 1302.2 0 1001 113 1433 1302.2 0 1433 114 609 1302.2 0 609 115 234 1302.2 0 234 116 126 1302.2 0 126 117 108 1302.2 0 108 118 125 1302.2 0 125 119 170 1302.2 0 170 120 332 1302.2 0 332 121 270 1302.2 0 270 122 198 1302.2 0 198 123 238 1302.2 0 238 124 169 1302.2 0 169 125 283 1302.2 0 283 126 214 1302.2 0 214 127 274 1302.2 0 274 128 208 1302.2 0 208 129 613 1302.2 0 613 130 444 1302.2 0 444 131 1052 1302.2 0 1052 132 450 1302.2 0 450 133 738 1302.2 0 738 134 567 1302.2 0 567 135 396 1302.2 0 396 136 353 1302.2 0 353 137 349 1302.2 0 349 138 478 1302.2 0 478 139 503 1302.2 0 503 140 621 1302.2 0 621 141 379 1302.2 0 379 142 568 1302.2 0 568 143 732 1302.2 0 732 144 1262 1302.2 0 1262 145 636 1302.2 0 636 146 418 1302.2 0 418 147 856 1302.2 0 856 148 484 1302.2 0 484 149 434 1302.2 0 434 150 292 1302.2 0 292 151 142 1302.2 0 142 152 106 1302.2 0 106 153 96 1302.2 0 96 154 107 1302.2 0 107 155 193 1302.2 0 193 156 242 1302.2 0 242 157 274 1302.2 0 274 158 407 1302.2 0 407 159 449 1302.2 0 449 160 464 1302.2 0 464 161 344 1302.2 0 344 162 360 1302.2 0 360 163 299 1302.2 0 299 164 346 1302.2 0 346 165 399 1302.2 0 399 166 378 1302.2 0 378 167 617 1302.2 0 617 168 563 1302.2 0 563 169 647 1302.2 0 647 170 315 1302.2 0 315 171 571 1302.2 0 571 172 238 1302.2 0 238 173 246 1302.2 0 246 174 215 1302.2 0 215 175 558 1302.2 0 558 176 365 1302.2 0 365 177 341 1302.2 0 341 178 214 1302.2 0 214 179 159 1302.2 0 159 180 190 1302.2 0 190 181 367 1302.2 0 367 182 532 1302.2 0 532 183 489 1302.2 0 489 184 524 1302.2 0 524 185 677 1302.2 0 677 186 611 1302.2 0 611 187 624 1302.2 0 624 188 459 1302.2 0 459 189 276 1302.2 0 276 190 335 1302.2 0 335 191 173 1302.2 0 173 192 338 1302.2 0 338 193 1222 1302.2 0 1222 194 800 1302.2 0 800 195 1491 1302.2 0 1491 196 410 1302.2 0 410 197 270 1302.2 0 270 198 257 1302.2 0 257 199 487 1302.2 0 487 200 610 1302.2 0 610 201 415 1302.2 0 415 202 127 1302.2 0 127 203 103 1302.2 0 103 204 300 1302.2 0 300 205 713 1302.2 0 713 206 1010 1302.2 0 1010 207 1307 1302.2 0 1307 208 716 1302.2 0 716 209 385 1302.2 0 385 210 292 1302.2 0 292 211 340 1302.2 0 340 212 598 1302.2 0 598 213 756 1302.2 0 756 214 736 1302.2 0 736 215 712 1302.2 0 712 216 607 1302.2 0 607 217 536 1302.2 0 536 218 764 1302.2 0 764 219 1041 1302.2 0 1041 220 788 1302.2 0 788 221 792 1302.2 0 792 222 309 1302.2 0 309 223 241 1302.2 0 241 224 578 1302.2 0 578 225 681 1302.2 0 681 226 807 1302.2 0 807 227 801 1302.2 0 801 228 928 1302.2 0 928 229 1239 1302.2 0 1239 230 1229 1302.2 0 1229 231 786 1302.2 0 786 232 687 1302.2 0 687 233 347 1302.2 0 347 234 342 1302.2 0 342 235 421 1302.2 0 421 236 1013 1302.2 0 1013 237 1066 1302.2 0 1066 238 934 1302.2 0 934 239 1236 1302.2 0 1236 240 1014 1302.2 0 1014 241 636 1302.2 0 636 242 255 1302.2 0 255 243 314 1302.2 0 314 244 404 1302.2 0 404 245 728 1302.2 0 728 246 1103 1302.2 0 1103 247 855 1302.2 0 855 248 998 1302.2 0 998 249 379 1302.2 0 379 250 273 1302.2 0 273 251 97 1302.2 0 97 252 177 1302.2 0 177 253 293 1302.2 0 293 254 793 1302.2 0 793 255 746 1302.2 0 746 256 859 1302.2 0 859 257 885 1302.2 0 885 258 1349 1302.2 0 1349 259 851 1302.2 0 851 260 612 1302.2 0 612 261 345 1302.2 0 345 262 371 1302.2 0 371 263 555 1302.2 0 555 264 858 1302.2 0 858 265 754 1302.2 0 754 266 1090 1302.2 0 1090 267 1285 1302.2 0 1285 268 1545 1302.2 0 1545 269 1190 1302.2 0 1190 270 519 1302.2 0 519 271 156 1302.2 0 156 272 183 1302.2 0 183 273 375 1302.2 0 375 274 760 1302.2 0 760 275 1102 1302.2 0 1102 276 916 1302.2 0 916 277 706 1302.2 0 706 278 367 1302.2 0 367 279 649 1302.2 0 649 280 860 1302.2 0 860 281 1668 1302.2 0 1668 282 1106 1302.2 0 1106 283 873 1302.2 0 873 284 537 1302.2 0 537 285 624 1302.2 0 624 286 799 1302.2 0 799 287 1043 1302.2 0 1043 288 1366 1302.2 0 1366 289 773 1302.2 0 773 290 450 1302.2 0 450 291 272 1302.2 0 272 292 418 1302.2 0 418 293 690 1302.2 0 690 294 1332 1302.2 0 1332 295 1170 1302.2 0 1170 296 608 1302.2 0 608 297 198 1302.2 0 198 298 248 1302.2 0 248 299 264 1302.2 0 264 300 500 1302.2 0 500 301 953 1302.2 0 953 Finished in 4.91 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 104,637 Reads with adapters: 17,000 (16.2%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 16,982 (16.2%) Total basepairs processed: 23,991,490 bp Total written (filtered): 4,665,370 bp (19.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17000 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 5 0.0 3 0 0 5 16 145 0.0 3 0 140 4 1 17 16721 0.0 3 16324 394 3 18 47 0.0 3 9 33 4 1 19 11 0.0 3 2 0 6 3 20 14 0.0 3 1 0 0 13 25 2 0.0 3 1 1 27 1 0.0 3 1 29 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 65 2 0.0 3 0 0 0 2 66 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 8 0.0 3 0 0 0 8 159 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 192 11 0.0 3 0 0 0 11 281 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.76 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 16,982 Reads with adapters: 538 (3.2%) Reads written (passing filters): 16,982 (100.0%) Total basepairs processed: 4,665,370 bp Total written (filtered): 4,663,753 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 538 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.1% C: 8.9% G: 44.4% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 535 265.3 0 535 4 3 66.3 0 3