This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 174.45 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 83,373 (1.6%) Reads that were too short: 34,781 (0.7%) Reads written (passing filters): 48,592 (0.9%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 9,363,619 bp (0.6%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 83373 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 127 1302.2 0 127 7 277 1302.2 0 277 8 262 1302.2 0 262 9 10362 1302.2 0 10362 10 28 1302.2 0 28 11 16 1302.2 0 16 12 502 1302.2 0 502 13 69 1302.2 0 69 14 8 1302.2 0 8 15 11 1302.2 0 11 16 17 1302.2 0 17 17 12 1302.2 0 12 18 10 1302.2 0 10 19 8 1302.2 0 8 20 11 1302.2 0 11 21 12 1302.2 0 12 22 11 1302.2 0 11 23 5 1302.2 0 5 24 8 1302.2 0 8 25 7 1302.2 0 7 26 11 1302.2 0 11 27 8 1302.2 0 8 28 12 1302.2 0 12 29 12 1302.2 0 12 30 45 1302.2 0 45 31 10 1302.2 0 10 32 12 1302.2 0 12 33 8 1302.2 0 8 34 7 1302.2 0 7 35 8 1302.2 0 8 36 12 1302.2 0 12 37 27 1302.2 0 27 38 15 1302.2 0 15 39 29 1302.2 0 29 40 39 1302.2 0 39 41 13 1302.2 0 13 42 13 1302.2 0 13 43 11 1302.2 0 11 44 18 1302.2 0 18 45 9 1302.2 0 9 46 15 1302.2 0 15 47 11 1302.2 0 11 48 16 1302.2 0 16 49 14 1302.2 0 14 50 11 1302.2 0 11 51 9 1302.2 0 9 52 13 1302.2 0 13 53 9 1302.2 0 9 54 10 1302.2 0 10 55 18 1302.2 0 18 56 15 1302.2 0 15 57 15 1302.2 0 15 58 10 1302.2 0 10 59 19 1302.2 0 19 60 10 1302.2 0 10 61 8 1302.2 0 8 62 8 1302.2 0 8 63 7 1302.2 0 7 64 12 1302.2 0 12 65 15 1302.2 0 15 66 13 1302.2 0 13 67 15 1302.2 0 15 68 5 1302.2 0 5 69 48 1302.2 0 48 70 20 1302.2 0 20 71 198 1302.2 0 198 72 16 1302.2 0 16 73 37 1302.2 0 37 74 82 1302.2 0 82 75 198 1302.2 0 198 76 90 1302.2 0 90 77 182 1302.2 0 182 78 279 1302.2 0 279 79 823 1302.2 0 823 80 464 1302.2 0 464 81 390 1302.2 0 390 82 1749 1302.2 0 1749 83 802 1302.2 0 802 84 731 1302.2 0 731 85 163 1302.2 0 163 86 294 1302.2 0 294 87 348 1302.2 0 348 88 298 1302.2 0 298 89 358 1302.2 0 358 90 157 1302.2 0 157 91 236 1302.2 0 236 92 86 1302.2 0 86 93 328 1302.2 0 328 94 200 1302.2 0 200 95 99 1302.2 0 99 96 68 1302.2 0 68 97 99 1302.2 0 99 98 61 1302.2 0 61 99 79 1302.2 0 79 100 49 1302.2 0 49 101 87 1302.2 0 87 102 34 1302.2 0 34 103 44 1302.2 0 44 104 67 1302.2 0 67 105 27 1302.2 0 27 106 27 1302.2 0 27 107 41 1302.2 0 41 108 56 1302.2 0 56 109 32 1302.2 0 32 110 59 1302.2 0 59 111 95 1302.2 0 95 112 43 1302.2 0 43 113 90 1302.2 0 90 114 90 1302.2 0 90 115 68 1302.2 0 68 116 87 1302.2 0 87 117 321 1302.2 0 321 118 266 1302.2 0 266 119 465 1302.2 0 465 120 334 1302.2 0 334 121 499 1302.2 0 499 122 287 1302.2 0 287 123 254 1302.2 0 254 124 222 1302.2 0 222 125 340 1302.2 0 340 126 174 1302.2 0 174 127 149 1302.2 0 149 128 174 1302.2 0 174 129 120 1302.2 0 120 130 130 1302.2 0 130 131 201 1302.2 0 201 132 387 1302.2 0 387 133 263 1302.2 0 263 134 262 1302.2 0 262 135 247 1302.2 0 247 136 278 1302.2 0 278 137 260 1302.2 0 260 138 267 1302.2 0 267 139 237 1302.2 0 237 140 188 1302.2 0 188 141 98 1302.2 0 98 142 184 1302.2 0 184 143 314 1302.2 0 314 144 355 1302.2 0 355 145 300 1302.2 0 300 146 260 1302.2 0 260 147 148 1302.2 0 148 148 219 1302.2 0 219 149 102 1302.2 0 102 150 140 1302.2 0 140 151 144 1302.2 0 144 152 216 1302.2 0 216 153 363 1302.2 0 363 154 398 1302.2 0 398 155 294 1302.2 0 294 156 235 1302.2 0 235 157 236 1302.2 0 236 158 306 1302.2 0 306 159 365 1302.2 0 365 160 185 1302.2 0 185 161 213 1302.2 0 213 162 205 1302.2 0 205 163 350 1302.2 0 350 164 405 1302.2 0 405 165 326 1302.2 0 326 166 267 1302.2 0 267 167 746 1302.2 0 746 168 576 1302.2 0 576 169 1028 1302.2 0 1028 170 290 1302.2 0 290 171 611 1302.2 0 611 172 625 1302.2 0 625 173 338 1302.2 0 338 174 259 1302.2 0 259 175 82 1302.2 0 82 176 119 1302.2 0 119 177 154 1302.2 0 154 178 276 1302.2 0 276 179 427 1302.2 0 427 180 410 1302.2 0 410 181 322 1302.2 0 322 182 275 1302.2 0 275 183 289 1302.2 0 289 184 350 1302.2 0 350 185 229 1302.2 0 229 186 136 1302.2 0 136 187 95 1302.2 0 95 188 288 1302.2 0 288 189 698 1302.2 0 698 190 747 1302.2 0 747 191 306 1302.2 0 306 192 208 1302.2 0 208 193 264 1302.2 0 264 194 109 1302.2 0 109 195 186 1302.2 0 186 196 420 1302.2 0 420 197 209 1302.2 0 209 198 140 1302.2 0 140 199 128 1302.2 0 128 200 420 1302.2 0 420 201 1540 1302.2 0 1540 202 1129 1302.2 0 1129 203 379 1302.2 0 379 204 329 1302.2 0 329 205 187 1302.2 0 187 206 128 1302.2 0 128 207 137 1302.2 0 137 208 365 1302.2 0 365 209 245 1302.2 0 245 210 205 1302.2 0 205 211 157 1302.2 0 157 212 221 1302.2 0 221 213 233 1302.2 0 233 214 216 1302.2 0 216 215 433 1302.2 0 433 216 510 1302.2 0 510 217 488 1302.2 0 488 218 287 1302.2 0 287 219 139 1302.2 0 139 220 151 1302.2 0 151 221 218 1302.2 0 218 222 497 1302.2 0 497 223 489 1302.2 0 489 224 596 1302.2 0 596 225 384 1302.2 0 384 226 391 1302.2 0 391 227 679 1302.2 0 679 228 1449 1302.2 0 1449 229 775 1302.2 0 775 230 1250 1302.2 0 1250 231 102 1302.2 0 102 232 184 1302.2 0 184 233 192 1302.2 0 192 234 188 1302.2 0 188 235 147 1302.2 0 147 236 141 1302.2 0 141 237 108 1302.2 0 108 238 143 1302.2 0 143 239 135 1302.2 0 135 240 179 1302.2 0 179 241 191 1302.2 0 191 242 289 1302.2 0 289 243 618 1302.2 0 618 244 560 1302.2 0 560 245 351 1302.2 0 351 246 113 1302.2 0 113 247 126 1302.2 0 126 248 104 1302.2 0 104 249 164 1302.2 0 164 250 431 1302.2 0 431 251 578 1302.2 0 578 252 572 1302.2 0 572 253 940 1302.2 0 940 254 814 1302.2 0 814 255 306 1302.2 0 306 256 138 1302.2 0 138 257 169 1302.2 0 169 258 224 1302.2 0 224 259 158 1302.2 0 158 260 146 1302.2 0 146 261 536 1302.2 0 536 262 1127 1302.2 0 1127 263 2016 1302.2 0 2016 264 870 1302.2 0 870 265 467 1302.2 0 467 266 217 1302.2 0 217 267 98 1302.2 0 98 268 179 1302.2 0 179 269 743 1302.2 0 743 270 708 1302.2 0 708 271 449 1302.2 0 449 272 228 1302.2 0 228 273 137 1302.2 0 137 274 145 1302.2 0 145 275 165 1302.2 0 165 276 184 1302.2 0 184 277 285 1302.2 0 285 278 422 1302.2 0 422 279 210 1302.2 0 210 280 163 1302.2 0 163 281 156 1302.2 0 156 282 297 1302.2 0 297 283 386 1302.2 0 386 284 326 1302.2 0 326 285 160 1302.2 0 160 286 145 1302.2 0 145 287 169 1302.2 0 169 288 113 1302.2 0 113 289 127 1302.2 0 127 290 274 1302.2 0 274 291 335 1302.2 0 335 292 370 1302.2 0 370 293 197 1302.2 0 197 294 171 1302.2 0 171 295 83 1302.2 0 83 296 98 1302.2 0 98 297 146 1302.2 0 146 298 193 1302.2 0 193 299 149 1302.2 0 149 300 322 1302.2 0 322 301 37 1302.2 0 37 Finished in 2.31 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 48,592 Reads with adapters: 10,762 (22.1%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 10,748 (22.1%) Total basepairs processed: 9,363,619 bp Total written (filtered): 2,922,891 bp (31.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10762 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 103 0.0 3 0 95 6 2 20 10584 0.0 4 10293 244 36 5 6 21 29 0.0 4 3 24 1 1 22 11 0.0 4 3 0 5 2 1 23 9 0.0 4 0 0 0 9 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 1 75 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 138 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 1 203 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 10,748 Reads with adapters: 1,980 (18.4%) Reads written (passing filters): 10,748 (100.0%) Total basepairs processed: 2,922,891 bp Total written (filtered): 2,672,016 bp (91.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1980 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 48.8% C: 1.2% G: 49.0% T: 0.9% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 3 1 167.9 0 1 4 2 42.0 0 2 8 2 0.2 0 2 9 197 0.0 0 13 184 10 141 0.0 1 3 138 11 697 0.0 1 3 694 12 4 0.0 1 0 4 13 1 0.0 1 0 1 15 1 0.0 1 0 1 22 1 0.0 1 1 256 2 0.0 1 2 257 875 0.0 1 823 52 258 34 0.0 1 31 3 259 10 0.0 1 10 260 6 0.0 1 6 263 4 0.0 1 4 267 1 0.0 1 1 272 1 0.0 1 1