This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 175.67 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 715,009 (13.4%) Reads that were too short: 305,890 (5.7%) Reads written (passing filters): 409,119 (7.7%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 70,521,706 bp (4.4%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 715009 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 361 1302.2 0 361 7 17 1302.2 0 17 8 197 1302.2 0 197 9 1127 1302.2 0 1127 10 13 1302.2 0 13 11 11 1302.2 0 11 12 31 1302.2 0 31 13 6 1302.2 0 6 14 10 1302.2 0 10 15 14 1302.2 0 14 16 11 1302.2 0 11 17 14 1302.2 0 14 18 12 1302.2 0 12 19 13 1302.2 0 13 20 11 1302.2 0 11 21 9 1302.2 0 9 22 17 1302.2 0 17 23 11 1302.2 0 11 24 28 1302.2 0 28 25 36 1302.2 0 36 26 21 1302.2 0 21 27 16 1302.2 0 16 28 9 1302.2 0 9 29 11 1302.2 0 11 30 24 1302.2 0 24 31 17 1302.2 0 17 32 18 1302.2 0 18 33 9 1302.2 0 9 34 13 1302.2 0 13 35 28 1302.2 0 28 36 12 1302.2 0 12 37 25 1302.2 0 25 38 14 1302.2 0 14 39 37 1302.2 0 37 40 16 1302.2 0 16 41 22 1302.2 0 22 42 70 1302.2 0 70 43 16 1302.2 0 16 44 13 1302.2 0 13 45 6 1302.2 0 6 46 7 1302.2 0 7 47 15 1302.2 0 15 48 16 1302.2 0 16 49 25 1302.2 0 25 50 16 1302.2 0 16 51 35 1302.2 0 35 52 126 1302.2 0 126 53 448 1302.2 0 448 54 18431 1302.2 0 18431 55 302 1302.2 0 302 56 28 1302.2 0 28 57 34 1302.2 0 34 58 23 1302.2 0 23 59 26 1302.2 0 26 60 85 1302.2 0 85 61 67 1302.2 0 67 62 78 1302.2 0 78 63 169 1302.2 0 169 64 268 1302.2 0 268 65 531 1302.2 0 531 66 530 1302.2 0 530 67 900 1302.2 0 900 68 2315 1302.2 0 2315 69 48677 1302.2 0 48677 70 1157 1302.2 0 1157 71 6851 1302.2 0 6851 72 49 1302.2 0 49 73 68 1302.2 0 68 74 139 1302.2 0 139 75 550 1302.2 0 550 76 278 1302.2 0 278 77 112 1302.2 0 112 78 551 1302.2 0 551 79 673 1302.2 0 673 80 1281 1302.2 0 1281 81 1655 1302.2 0 1655 82 1477 1302.2 0 1477 83 480 1302.2 0 480 84 1556 1302.2 0 1556 85 3076 1302.2 0 3076 86 1381 1302.2 0 1381 87 1590 1302.2 0 1590 88 18729 1302.2 0 18729 89 909 1302.2 0 909 90 487 1302.2 0 487 91 2273 1302.2 0 2273 92 1326 1302.2 0 1326 93 373 1302.2 0 373 94 2507 1302.2 0 2507 95 1425 1302.2 0 1425 96 2318 1302.2 0 2318 97 3128 1302.2 0 3128 98 2407 1302.2 0 2407 99 1048 1302.2 0 1048 100 1380 1302.2 0 1380 101 2952 1302.2 0 2952 102 10557 1302.2 0 10557 103 1577 1302.2 0 1577 104 1761 1302.2 0 1761 105 971 1302.2 0 971 106 353 1302.2 0 353 107 558 1302.2 0 558 108 1773 1302.2 0 1773 109 607 1302.2 0 607 110 228 1302.2 0 228 111 561 1302.2 0 561 112 1326 1302.2 0 1326 113 1036 1302.2 0 1036 114 2076 1302.2 0 2076 115 852 1302.2 0 852 116 1053 1302.2 0 1053 117 1986 1302.2 0 1986 118 1873 1302.2 0 1873 119 1609 1302.2 0 1609 120 3497 1302.2 0 3497 121 2008 1302.2 0 2008 122 2024 1302.2 0 2024 123 2092 1302.2 0 2092 124 1952 1302.2 0 1952 125 1869 1302.2 0 1869 126 2162 1302.2 0 2162 127 1675 1302.2 0 1675 128 1667 1302.2 0 1667 129 2517 1302.2 0 2517 130 1760 1302.2 0 1760 131 1937 1302.2 0 1937 132 3004 1302.2 0 3004 133 4889 1302.2 0 4889 134 3675 1302.2 0 3675 135 2036 1302.2 0 2036 136 2902 1302.2 0 2902 137 3856 1302.2 0 3856 138 4923 1302.2 0 4923 139 3241 1302.2 0 3241 140 3139 1302.2 0 3139 141 1716 1302.2 0 1716 142 2113 1302.2 0 2113 143 3074 1302.2 0 3074 144 1729 1302.2 0 1729 145 1371 1302.2 0 1371 146 1650 1302.2 0 1650 147 3209 1302.2 0 3209 148 4165 1302.2 0 4165 149 2949 1302.2 0 2949 150 3099 1302.2 0 3099 151 2326 1302.2 0 2326 152 2277 1302.2 0 2277 153 1606 1302.2 0 1606 154 1434 1302.2 0 1434 155 2077 1302.2 0 2077 156 2579 1302.2 0 2579 157 2437 1302.2 0 2437 158 3503 1302.2 0 3503 159 3783 1302.2 0 3783 160 3676 1302.2 0 3676 161 2827 1302.2 0 2827 162 2497 1302.2 0 2497 163 2521 1302.2 0 2521 164 2445 1302.2 0 2445 165 2245 1302.2 0 2245 166 2011 1302.2 0 2011 167 2338 1302.2 0 2338 168 3325 1302.2 0 3325 169 4178 1302.2 0 4178 170 2496 1302.2 0 2496 171 3725 1302.2 0 3725 172 3488 1302.2 0 3488 173 1812 1302.2 0 1812 174 1474 1302.2 0 1474 175 3412 1302.2 0 3412 176 8292 1302.2 0 8292 177 6889 1302.2 0 6889 178 3525 1302.2 0 3525 179 1939 1302.2 0 1939 180 1726 1302.2 0 1726 181 2419 1302.2 0 2419 182 2898 1302.2 0 2898 183 3360 1302.2 0 3360 184 3014 1302.2 0 3014 185 3033 1302.2 0 3033 186 3689 1302.2 0 3689 187 4508 1302.2 0 4508 188 5844 1302.2 0 5844 189 6933 1302.2 0 6933 190 1875 1302.2 0 1875 191 1363 1302.2 0 1363 192 1348 1302.2 0 1348 193 3139 1302.2 0 3139 194 3038 1302.2 0 3038 195 3371 1302.2 0 3371 196 4967 1302.2 0 4967 197 2434 1302.2 0 2434 198 1256 1302.2 0 1256 199 1873 1302.2 0 1873 200 3595 1302.2 0 3595 201 3958 1302.2 0 3958 202 2465 1302.2 0 2465 203 1491 1302.2 0 1491 204 2154 1302.2 0 2154 205 3105 1302.2 0 3105 206 2596 1302.2 0 2596 207 2655 1302.2 0 2655 208 4889 1302.2 0 4889 209 2478 1302.2 0 2478 210 1715 1302.2 0 1715 211 1872 1302.2 0 1872 212 2499 1302.2 0 2499 213 4092 1302.2 0 4092 214 3297 1302.2 0 3297 215 3629 1302.2 0 3629 216 3316 1302.2 0 3316 217 2947 1302.2 0 2947 218 3309 1302.2 0 3309 219 3912 1302.2 0 3912 220 3083 1302.2 0 3083 221 3765 1302.2 0 3765 222 4184 1302.2 0 4184 223 2546 1302.2 0 2546 224 2437 1302.2 0 2437 225 3059 1302.2 0 3059 226 2739 1302.2 0 2739 227 2490 1302.2 0 2490 228 2418 1302.2 0 2418 229 3943 1302.2 0 3943 230 3914 1302.2 0 3914 231 2650 1302.2 0 2650 232 2575 1302.2 0 2575 233 3513 1302.2 0 3513 234 1616 1302.2 0 1616 235 1718 1302.2 0 1718 236 3087 1302.2 0 3087 237 4136 1302.2 0 4136 238 3506 1302.2 0 3506 239 3248 1302.2 0 3248 240 2824 1302.2 0 2824 241 2720 1302.2 0 2720 242 2737 1302.2 0 2737 243 2846 1302.2 0 2846 244 4405 1302.2 0 4405 245 3551 1302.2 0 3551 246 3176 1302.2 0 3176 247 2526 1302.2 0 2526 248 3334 1302.2 0 3334 249 3799 1302.2 0 3799 250 3483 1302.2 0 3483 251 2014 1302.2 0 2014 252 1958 1302.2 0 1958 253 2194 1302.2 0 2194 254 2669 1302.2 0 2669 255 2828 1302.2 0 2828 256 2490 1302.2 0 2490 257 2187 1302.2 0 2187 258 2875 1302.2 0 2875 259 3921 1302.2 0 3921 260 2809 1302.2 0 2809 261 2915 1302.2 0 2915 262 2053 1302.2 0 2053 263 2401 1302.2 0 2401 264 1756 1302.2 0 1756 265 1528 1302.2 0 1528 266 2021 1302.2 0 2021 267 2759 1302.2 0 2759 268 4699 1302.2 0 4699 269 5713 1302.2 0 5713 270 5217 1302.2 0 5217 271 4782 1302.2 0 4782 272 3178 1302.2 0 3178 273 2787 1302.2 0 2787 274 2662 1302.2 0 2662 275 3776 1302.2 0 3776 276 4651 1302.2 0 4651 277 3942 1302.2 0 3942 278 2774 1302.2 0 2774 279 2415 1302.2 0 2415 280 2489 1302.2 0 2489 281 4668 1302.2 0 4668 282 4321 1302.2 0 4321 283 3823 1302.2 0 3823 284 3610 1302.2 0 3610 285 2349 1302.2 0 2349 286 1615 1302.2 0 1615 287 2260 1302.2 0 2260 288 3051 1302.2 0 3051 289 4015 1302.2 0 4015 290 2863 1302.2 0 2863 291 3331 1302.2 0 3331 292 2714 1302.2 0 2714 293 2751 1302.2 0 2751 294 3569 1302.2 0 3569 295 4677 1302.2 0 4677 296 3374 1302.2 0 3374 297 2476 1302.2 0 2476 298 1778 1302.2 0 1778 299 2270 1302.2 0 2270 300 2164 1302.2 0 2164 301 5299 1302.2 0 5299 Finished in 18.18 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 409,119 Reads with adapters: 1,336 (0.3%) Reads that were too short: 172 (0.0%) Reads written (passing filters): 1,164 (0.3%) Total basepairs processed: 70,521,706 bp Total written (filtered): 306,684 bp (0.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1336 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 1 0.0 3 0 0 0 0 1 19 14 0.0 3 0 7 1 0 6 20 1063 0.0 4 1022 31 4 3 3 21 13 0.0 4 5 2 0 2 4 22 6 0.0 4 2 0 1 0 3 23 2 0.0 4 0 0 0 1 1 24 9 0.0 4 0 0 0 0 9 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 1 28 1 0.0 4 0 0 0 0 1 30 2 0.0 4 0 0 0 0 2 31 1 0.0 4 0 0 0 0 1 33 3 0.0 4 0 0 0 0 3 37 2 0.0 4 0 0 0 0 2 41 2 0.0 4 0 0 0 0 2 42 3 0.0 4 0 0 0 0 3 43 2 0.0 4 0 0 0 0 2 46 2 0.0 4 0 0 0 0 2 48 3 0.0 4 0 0 0 0 3 49 3 0.0 4 0 0 0 0 3 51 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 53 2 0.0 4 0 0 0 0 2 54 6 0.0 4 0 0 0 1 5 55 2 0.0 4 0 0 0 0 2 56 3 0.0 4 0 0 0 0 3 59 1 0.0 4 0 0 0 0 1 60 4 0.0 4 0 0 0 0 4 61 1 0.0 4 0 0 0 0 1 62 2 0.0 4 0 0 0 0 2 63 1 0.0 4 0 0 0 0 1 64 2 0.0 4 0 0 0 0 2 65 4 0.0 4 0 0 0 0 4 66 3 0.0 4 0 0 0 0 3 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 4 0.0 4 0 0 0 0 4 72 3 0.0 4 0 0 0 0 3 73 2 0.0 4 0 0 0 0 2 74 1 0.0 4 0 0 0 0 1 75 3 0.0 4 0 0 0 0 3 78 6 0.0 4 0 0 0 0 6 79 1 0.0 4 0 0 0 0 1 80 4 0.0 4 0 0 0 0 4 81 1 0.0 4 0 0 0 0 1 83 4 0.0 4 0 0 0 0 4 84 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 87 4 0.0 4 0 0 0 0 4 88 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 3 0.0 4 0 0 0 2 1 93 4 0.0 4 0 0 0 0 4 94 1 0.0 4 0 0 0 0 1 95 3 0.0 4 0 0 0 0 3 96 3 0.0 4 0 0 0 0 3 97 1 0.0 4 0 0 0 0 1 98 3 0.0 4 0 0 0 0 3 99 4 0.0 4 0 0 0 0 4 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 3 0.0 4 0 0 0 0 3 103 1 0.0 4 0 0 0 0 1 104 5 0.0 4 0 0 0 0 5 105 1 0.0 4 0 0 0 0 1 106 4 0.0 4 0 0 0 1 3 107 1 0.0 4 0 0 0 0 1 108 3 0.0 4 0 0 0 0 3 109 4 0.0 4 0 0 0 0 4 112 2 0.0 4 0 0 0 1 1 113 1 0.0 4 0 0 0 0 1 115 2 0.0 4 0 0 0 0 2 116 1 0.0 4 0 0 0 0 1 117 2 0.0 4 0 0 0 0 2 118 2 0.0 4 0 0 0 0 2 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 123 3 0.0 4 0 0 0 0 3 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 128 3 0.0 4 0 0 0 0 3 129 2 0.0 4 0 0 0 1 1 132 2 0.0 4 0 0 0 0 2 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 3 0.0 4 0 0 0 0 3 140 2 0.0 4 0 0 0 0 2 142 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 144 2 0.0 4 0 0 0 0 2 148 1 0.0 4 0 0 0 0 1 150 2 0.0 4 0 0 0 0 2 151 3 0.0 4 0 0 0 0 3 152 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 161 3 0.0 4 0 0 0 0 3 162 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 1 170 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 173 2 0.0 4 0 0 0 0 2 174 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 176 2 0.0 4 0 0 0 0 2 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 1,164 Reads with adapters: 119 (10.2%) Reads written (passing filters): 1,164 (100.0%) Total basepairs processed: 306,684 bp Total written (filtered): 304,268 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 119 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.0% C: 3.4% G: 86.6% T: 4.2% none/other: 0.8% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 10 0.0 0 0 10 10 25 0.0 1 1 24 11 79 0.0 1 0 79 150 1 0.0 1 1 257 2 0.0 1 2 271 1 0.0 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.