This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 167.60 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 162,095 (3.0%) Reads that were too short: 22,600 (0.4%) Reads written (passing filters): 139,495 (2.6%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 29,351,989 bp (1.8%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 162095 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1253 1302.2 0 1253 7 444 1302.2 0 444 8 1422 1302.2 0 1422 9 3536 1302.2 0 3536 10 3717 1302.2 0 3717 11 19 1302.2 0 19 12 88 1302.2 0 88 13 11 1302.2 0 11 14 5 1302.2 0 5 15 8 1302.2 0 8 16 13 1302.2 0 13 17 4 1302.2 0 4 18 5 1302.2 0 5 19 13 1302.2 0 13 20 3 1302.2 0 3 21 10 1302.2 0 10 22 12 1302.2 0 12 23 10 1302.2 0 10 24 7 1302.2 0 7 25 6 1302.2 0 6 26 9 1302.2 0 9 27 9 1302.2 0 9 28 6 1302.2 0 6 29 6 1302.2 0 6 30 10 1302.2 0 10 31 9 1302.2 0 9 32 16 1302.2 0 16 33 8 1302.2 0 8 34 7 1302.2 0 7 35 11 1302.2 0 11 36 8 1302.2 0 8 37 20 1302.2 0 20 38 7 1302.2 0 7 39 29 1302.2 0 29 40 111 1302.2 0 111 41 8 1302.2 0 8 42 9 1302.2 0 9 43 4 1302.2 0 4 44 10 1302.2 0 10 45 236 1302.2 0 236 46 87 1302.2 0 87 47 22 1302.2 0 22 48 15 1302.2 0 15 49 10 1302.2 0 10 50 16 1302.2 0 16 51 13 1302.2 0 13 52 29 1302.2 0 29 53 16 1302.2 0 16 54 31 1302.2 0 31 55 10 1302.2 0 10 56 6 1302.2 0 6 57 19 1302.2 0 19 58 17 1302.2 0 17 59 78 1302.2 0 78 60 40 1302.2 0 40 61 3 1302.2 0 3 62 25 1302.2 0 25 63 35 1302.2 0 35 64 38 1302.2 0 38 65 43 1302.2 0 43 66 42 1302.2 0 42 67 14 1302.2 0 14 68 67 1302.2 0 67 69 41 1302.2 0 41 70 31 1302.2 0 31 71 23 1302.2 0 23 72 69 1302.2 0 69 73 54 1302.2 0 54 74 70 1302.2 0 70 75 127 1302.2 0 127 76 142 1302.2 0 142 77 123 1302.2 0 123 78 304 1302.2 0 304 79 769 1302.2 0 769 80 179 1302.2 0 179 81 292 1302.2 0 292 82 812 1302.2 0 812 83 405 1302.2 0 405 84 341 1302.2 0 341 85 681 1302.2 0 681 86 716 1302.2 0 716 87 1219 1302.2 0 1219 88 493 1302.2 0 493 89 525 1302.2 0 525 90 740 1302.2 0 740 91 970 1302.2 0 970 92 9457 1302.2 0 9457 93 55062 1302.2 0 55062 94 45587 1302.2 0 45587 95 605 1302.2 0 605 96 75 1302.2 0 75 97 12 1302.2 0 12 98 11 1302.2 0 11 99 10 1302.2 0 10 100 10 1302.2 0 10 101 11 1302.2 0 11 102 30 1302.2 0 30 103 7 1302.2 0 7 104 7 1302.2 0 7 105 16 1302.2 0 16 106 24 1302.2 0 24 107 156 1302.2 0 156 108 86 1302.2 0 86 109 18 1302.2 0 18 110 13 1302.2 0 13 111 97 1302.2 0 97 112 71 1302.2 0 71 113 9 1302.2 0 9 114 32 1302.2 0 32 115 53 1302.2 0 53 116 45 1302.2 0 45 117 33 1302.2 0 33 118 19 1302.2 0 19 119 16 1302.2 0 16 120 26 1302.2 0 26 121 16 1302.2 0 16 122 10 1302.2 0 10 123 14 1302.2 0 14 124 8 1302.2 0 8 125 18 1302.2 0 18 126 18 1302.2 0 18 127 23 1302.2 0 23 128 18 1302.2 0 18 129 14 1302.2 0 14 130 48 1302.2 0 48 131 76 1302.2 0 76 132 25 1302.2 0 25 133 18 1302.2 0 18 134 23 1302.2 0 23 135 20 1302.2 0 20 136 36 1302.2 0 36 137 50 1302.2 0 50 138 58 1302.2 0 58 139 54 1302.2 0 54 140 62 1302.2 0 62 141 128 1302.2 0 128 142 10 1302.2 0 10 143 14 1302.2 0 14 144 46 1302.2 0 46 145 13 1302.2 0 13 146 7 1302.2 0 7 147 14 1302.2 0 14 148 22 1302.2 0 22 149 17 1302.2 0 17 150 15 1302.2 0 15 151 13 1302.2 0 13 152 6 1302.2 0 6 153 5 1302.2 0 5 154 6 1302.2 0 6 155 8 1302.2 0 8 156 28 1302.2 0 28 157 11 1302.2 0 11 158 24 1302.2 0 24 159 15 1302.2 0 15 160 10 1302.2 0 10 161 13 1302.2 0 13 162 7 1302.2 0 7 163 9 1302.2 0 9 164 14 1302.2 0 14 165 15 1302.2 0 15 166 22 1302.2 0 22 167 15 1302.2 0 15 168 20 1302.2 0 20 169 37 1302.2 0 37 170 9 1302.2 0 9 171 12 1302.2 0 12 172 19 1302.2 0 19 173 35 1302.2 0 35 174 14 1302.2 0 14 175 37 1302.2 0 37 176 31 1302.2 0 31 177 312 1302.2 0 312 178 383 1302.2 0 383 179 849 1302.2 0 849 180 164 1302.2 0 164 181 17 1302.2 0 17 182 15 1302.2 0 15 183 26 1302.2 0 26 184 29 1302.2 0 29 185 53 1302.2 0 53 186 41 1302.2 0 41 187 43 1302.2 0 43 188 107 1302.2 0 107 189 68 1302.2 0 68 190 152 1302.2 0 152 191 136 1302.2 0 136 192 62 1302.2 0 62 193 58 1302.2 0 58 194 62 1302.2 0 62 195 70 1302.2 0 70 196 52 1302.2 0 52 197 54 1302.2 0 54 198 83 1302.2 0 83 199 130 1302.2 0 130 200 212 1302.2 0 212 201 2468 1302.2 0 2468 202 5316 1302.2 0 5316 203 6422 1302.2 0 6422 204 467 1302.2 0 467 205 294 1302.2 0 294 206 671 1302.2 0 671 207 135 1302.2 0 135 208 123 1302.2 0 123 209 63 1302.2 0 63 210 31 1302.2 0 31 211 38 1302.2 0 38 212 45 1302.2 0 45 213 34 1302.2 0 34 214 45 1302.2 0 45 215 40 1302.2 0 40 216 67 1302.2 0 67 217 63 1302.2 0 63 218 97 1302.2 0 97 219 34 1302.2 0 34 220 27 1302.2 0 27 221 44 1302.2 0 44 222 65 1302.2 0 65 223 155 1302.2 0 155 224 36 1302.2 0 36 225 201 1302.2 0 201 226 363 1302.2 0 363 227 544 1302.2 0 544 228 574 1302.2 0 574 229 272 1302.2 0 272 230 26 1302.2 0 26 231 18 1302.2 0 18 232 12 1302.2 0 12 233 13 1302.2 0 13 234 5 1302.2 0 5 235 15 1302.2 0 15 236 41 1302.2 0 41 237 42 1302.2 0 42 238 82 1302.2 0 82 239 7 1302.2 0 7 240 6 1302.2 0 6 241 2 1302.2 0 2 242 5 1302.2 0 5 243 8 1302.2 0 8 244 7 1302.2 0 7 245 12 1302.2 0 12 246 46 1302.2 0 46 247 17 1302.2 0 17 248 7 1302.2 0 7 249 8 1302.2 0 8 250 6 1302.2 0 6 251 5 1302.2 0 5 252 5 1302.2 0 5 253 14 1302.2 0 14 254 52 1302.2 0 52 255 87 1302.2 0 87 256 56 1302.2 0 56 257 27 1302.2 0 27 258 59 1302.2 0 59 259 119 1302.2 0 119 260 108 1302.2 0 108 261 84 1302.2 0 84 262 80 1302.2 0 80 263 33 1302.2 0 33 264 30 1302.2 0 30 265 44 1302.2 0 44 266 51 1302.2 0 51 267 21 1302.2 0 21 268 9 1302.2 0 9 269 12 1302.2 0 12 270 11 1302.2 0 11 271 22 1302.2 0 22 272 24 1302.2 0 24 273 36 1302.2 0 36 274 10 1302.2 0 10 275 13 1302.2 0 13 276 13 1302.2 0 13 277 24 1302.2 0 24 278 33 1302.2 0 33 279 52 1302.2 0 52 280 22 1302.2 0 22 281 11 1302.2 0 11 282 29 1302.2 0 29 283 13 1302.2 0 13 284 36 1302.2 0 36 285 162 1302.2 0 162 286 451 1302.2 0 451 287 3635 1302.2 0 3635 288 16 1302.2 0 16 289 4 1302.2 0 4 290 4 1302.2 0 4 291 2 1302.2 0 2 292 73 1302.2 0 73 293 276 1302.2 0 276 294 17 1302.2 0 17 295 13 1302.2 0 13 296 13 1302.2 0 13 297 4 1302.2 0 4 298 3 1302.2 0 3 299 11 1302.2 0 11 300 7 1302.2 0 7 301 13 1302.2 0 13 Finished in 6.11 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 139,495 Reads with adapters: 1,433 (1.0%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 1,408 (1.0%) Total basepairs processed: 29,351,989 bp Total written (filtered): 344,178 bp (1.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1433 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 7 0.0 3 0 6 0 1 17 1069 0.0 3 1053 15 1 18 24 0.0 3 4 2 17 1 19 33 0.0 3 0 0 7 26 20 5 0.0 3 0 0 0 5 39 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 74 3 0.0 3 0 0 0 3 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 1 1 90 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 134 0.0 3 0 0 0 134 100 30 0.0 3 0 0 0 30 101 2 0.0 3 0 0 0 2 102 77 0.0 3 0 0 0 77 103 8 0.0 3 0 0 0 8 112 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 144 1 0.0 3 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 200 12 0.0 3 0 0 0 12 201 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 1,408 Reads with adapters: 54 (3.8%) Reads written (passing filters): 1,408 (100.0%) Total basepairs processed: 344,178 bp Total written (filtered): 344,015 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 54 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.1% C: 3.7% G: 77.8% T: 7.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 53 22.0 0 53 4 1 5.5 0 1