This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 170.72 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 130,176 (2.4%) Reads that were too short: 36,089 (0.7%) Reads written (passing filters): 94,087 (1.8%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 21,248,069 bp (1.3%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 130176 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 401 1302.2 0 401 7 6973 1302.2 0 6973 8 389 1302.2 0 389 9 19197 1302.2 0 19197 10 41 1302.2 0 41 11 11 1302.2 0 11 12 13 1302.2 0 13 13 7 1302.2 0 7 14 11 1302.2 0 11 15 11 1302.2 0 11 16 10 1302.2 0 10 17 12 1302.2 0 12 18 16 1302.2 0 16 19 8 1302.2 0 8 20 12 1302.2 0 12 21 10 1302.2 0 10 22 15 1302.2 0 15 23 16 1302.2 0 16 24 28 1302.2 0 28 25 9 1302.2 0 9 26 12 1302.2 0 12 27 48 1302.2 0 48 28 12 1302.2 0 12 29 10 1302.2 0 10 30 22 1302.2 0 22 31 7 1302.2 0 7 32 14 1302.2 0 14 33 22 1302.2 0 22 34 77 1302.2 0 77 35 55 1302.2 0 55 36 39 1302.2 0 39 37 115 1302.2 0 115 38 31 1302.2 0 31 39 54 1302.2 0 54 40 20 1302.2 0 20 41 69 1302.2 0 69 42 36 1302.2 0 36 43 34 1302.2 0 34 44 29 1302.2 0 29 45 22 1302.2 0 22 46 24 1302.2 0 24 47 47 1302.2 0 47 48 110 1302.2 0 110 49 163 1302.2 0 163 50 232 1302.2 0 232 51 230 1302.2 0 230 52 429 1302.2 0 429 53 1143 1302.2 0 1143 54 16375 1302.2 0 16375 55 5019 1302.2 0 5019 56 8037 1302.2 0 8037 57 51 1302.2 0 51 58 14 1302.2 0 14 59 8 1302.2 0 8 60 9 1302.2 0 9 61 15 1302.2 0 15 62 9 1302.2 0 9 63 6 1302.2 0 6 64 10 1302.2 0 10 65 10 1302.2 0 10 66 8 1302.2 0 8 67 2 1302.2 0 2 68 7 1302.2 0 7 69 8 1302.2 0 8 70 7 1302.2 0 7 71 4 1302.2 0 4 72 8 1302.2 0 8 73 3 1302.2 0 3 74 6 1302.2 0 6 75 9 1302.2 0 9 76 7 1302.2 0 7 77 5 1302.2 0 5 78 13 1302.2 0 13 79 29 1302.2 0 29 80 46 1302.2 0 46 81 141 1302.2 0 141 82 79 1302.2 0 79 83 114 1302.2 0 114 84 177 1302.2 0 177 85 56 1302.2 0 56 86 24 1302.2 0 24 87 61 1302.2 0 61 88 244 1302.2 0 244 89 29 1302.2 0 29 90 10 1302.2 0 10 91 184 1302.2 0 184 92 264 1302.2 0 264 93 52 1302.2 0 52 94 319 1302.2 0 319 95 801 1302.2 0 801 96 890 1302.2 0 890 97 118 1302.2 0 118 98 410 1302.2 0 410 99 92 1302.2 0 92 100 36 1302.2 0 36 101 92 1302.2 0 92 102 124 1302.2 0 124 103 88 1302.2 0 88 104 168 1302.2 0 168 105 86 1302.2 0 86 106 10 1302.2 0 10 107 64 1302.2 0 64 108 206 1302.2 0 206 109 170 1302.2 0 170 110 87 1302.2 0 87 111 184 1302.2 0 184 112 44 1302.2 0 44 113 94 1302.2 0 94 114 89 1302.2 0 89 115 49 1302.2 0 49 116 176 1302.2 0 176 117 281 1302.2 0 281 118 145 1302.2 0 145 119 440 1302.2 0 440 120 402 1302.2 0 402 121 1029 1302.2 0 1029 122 694 1302.2 0 694 123 1111 1302.2 0 1111 124 174 1302.2 0 174 125 119 1302.2 0 119 126 121 1302.2 0 121 127 94 1302.2 0 94 128 180 1302.2 0 180 129 466 1302.2 0 466 130 195 1302.2 0 195 131 140 1302.2 0 140 132 147 1302.2 0 147 133 210 1302.2 0 210 134 199 1302.2 0 199 135 226 1302.2 0 226 136 222 1302.2 0 222 137 268 1302.2 0 268 138 214 1302.2 0 214 139 488 1302.2 0 488 140 251 1302.2 0 251 141 328 1302.2 0 328 142 276 1302.2 0 276 143 358 1302.2 0 358 144 102 1302.2 0 102 145 216 1302.2 0 216 146 186 1302.2 0 186 147 539 1302.2 0 539 148 293 1302.2 0 293 149 170 1302.2 0 170 150 227 1302.2 0 227 151 182 1302.2 0 182 152 257 1302.2 0 257 153 142 1302.2 0 142 154 112 1302.2 0 112 155 235 1302.2 0 235 156 254 1302.2 0 254 157 264 1302.2 0 264 158 285 1302.2 0 285 159 304 1302.2 0 304 160 382 1302.2 0 382 161 350 1302.2 0 350 162 503 1302.2 0 503 163 459 1302.2 0 459 164 215 1302.2 0 215 165 321 1302.2 0 321 166 405 1302.2 0 405 167 785 1302.2 0 785 168 452 1302.2 0 452 169 212 1302.2 0 212 170 145 1302.2 0 145 171 310 1302.2 0 310 172 431 1302.2 0 431 173 169 1302.2 0 169 174 181 1302.2 0 181 175 442 1302.2 0 442 176 1422 1302.2 0 1422 177 1208 1302.2 0 1208 178 1448 1302.2 0 1448 179 128 1302.2 0 128 180 175 1302.2 0 175 181 417 1302.2 0 417 182 351 1302.2 0 351 183 359 1302.2 0 359 184 302 1302.2 0 302 185 195 1302.2 0 195 186 204 1302.2 0 204 187 232 1302.2 0 232 188 421 1302.2 0 421 189 311 1302.2 0 311 190 141 1302.2 0 141 191 103 1302.2 0 103 192 150 1302.2 0 150 193 253 1302.2 0 253 194 287 1302.2 0 287 195 455 1302.2 0 455 196 498 1302.2 0 498 197 125 1302.2 0 125 198 81 1302.2 0 81 199 87 1302.2 0 87 200 241 1302.2 0 241 201 332 1302.2 0 332 202 207 1302.2 0 207 203 127 1302.2 0 127 204 176 1302.2 0 176 205 275 1302.2 0 275 206 197 1302.2 0 197 207 291 1302.2 0 291 208 350 1302.2 0 350 209 282 1302.2 0 282 210 213 1302.2 0 213 211 163 1302.2 0 163 212 242 1302.2 0 242 213 365 1302.2 0 365 214 329 1302.2 0 329 215 373 1302.2 0 373 216 302 1302.2 0 302 217 216 1302.2 0 216 218 202 1302.2 0 202 219 224 1302.2 0 224 220 397 1302.2 0 397 221 1160 1302.2 0 1160 222 514 1302.2 0 514 223 194 1302.2 0 194 224 221 1302.2 0 221 225 294 1302.2 0 294 226 315 1302.2 0 315 227 300 1302.2 0 300 228 316 1302.2 0 316 229 344 1302.2 0 344 230 258 1302.2 0 258 231 225 1302.2 0 225 232 748 1302.2 0 748 233 579 1302.2 0 579 234 192 1302.2 0 192 235 130 1302.2 0 130 236 257 1302.2 0 257 237 284 1302.2 0 284 238 226 1302.2 0 226 239 201 1302.2 0 201 240 224 1302.2 0 224 241 250 1302.2 0 250 242 329 1302.2 0 329 243 216 1302.2 0 216 244 201 1302.2 0 201 245 175 1302.2 0 175 246 175 1302.2 0 175 247 169 1302.2 0 169 248 482 1302.2 0 482 249 558 1302.2 0 558 250 627 1302.2 0 627 251 307 1302.2 0 307 252 199 1302.2 0 199 253 290 1302.2 0 290 254 406 1302.2 0 406 255 353 1302.2 0 353 256 285 1302.2 0 285 257 283 1302.2 0 283 258 407 1302.2 0 407 259 378 1302.2 0 378 260 624 1302.2 0 624 261 464 1302.2 0 464 262 961 1302.2 0 961 263 654 1302.2 0 654 264 656 1302.2 0 656 265 310 1302.2 0 310 266 260 1302.2 0 260 267 227 1302.2 0 227 268 266 1302.2 0 266 269 583 1302.2 0 583 270 458 1302.2 0 458 271 304 1302.2 0 304 272 236 1302.2 0 236 273 241 1302.2 0 241 274 233 1302.2 0 233 275 257 1302.2 0 257 276 410 1302.2 0 410 277 355 1302.2 0 355 278 323 1302.2 0 323 279 253 1302.2 0 253 280 481 1302.2 0 481 281 599 1302.2 0 599 282 1125 1302.2 0 1125 283 446 1302.2 0 446 284 324 1302.2 0 324 285 192 1302.2 0 192 286 201 1302.2 0 201 287 287 1302.2 0 287 288 532 1302.2 0 532 289 1477 1302.2 0 1477 290 986 1302.2 0 986 291 583 1302.2 0 583 292 362 1302.2 0 362 293 169 1302.2 0 169 294 419 1302.2 0 419 295 456 1302.2 0 456 296 248 1302.2 0 248 297 206 1302.2 0 206 298 385 1302.2 0 385 299 227 1302.2 0 227 300 172 1302.2 0 172 301 134 1302.2 0 134 Finished in 4.45 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 94,087 Reads with adapters: 19,957 (21.2%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 19,927 (21.2%) Total basepairs processed: 21,248,069 bp Total written (filtered): 5,425,708 bp (25.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19957 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 8 0.0 3 0 0 7 1 16 169 0.0 3 0 165 3 1 17 19291 0.0 3 18848 427 14 2 18 79 0.0 3 2 44 32 1 19 37 0.0 3 0 0 19 18 20 7 0.0 3 0 0 0 7 24 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 36 1 0.0 3 1 42 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 1 66 1 0.0 3 0 0 0 1 72 3 0.0 3 0 0 0 3 75 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 98 311 0.0 3 0 0 0 311 100 5 0.0 3 0 0 1 4 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 217 17 0.0 3 0 0 0 17 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.90 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 19,927 Reads with adapters: 1,158 (5.8%) Reads written (passing filters): 19,927 (100.0%) Total basepairs processed: 5,425,708 bp Total written (filtered): 5,422,227 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1158 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 69.9% C: 5.5% G: 23.3% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1151 311.4 0 1151 4 7 77.8 0 7