This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 175.90 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 145,512 (2.7%) Reads that were too short: 47,622 (0.9%) Reads written (passing filters): 97,890 (1.8%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 17,822,727 bp (1.1%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 145512 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 180 1302.2 0 180 7 119 1302.2 0 119 8 130 1302.2 0 130 9 3835 1302.2 0 3835 10 24 1302.2 0 24 11 20 1302.2 0 20 12 6 1302.2 0 6 13 11 1302.2 0 11 14 10 1302.2 0 10 15 32 1302.2 0 32 16 8 1302.2 0 8 17 27 1302.2 0 27 18 6 1302.2 0 6 19 12 1302.2 0 12 20 14 1302.2 0 14 21 17 1302.2 0 17 22 7 1302.2 0 7 23 7 1302.2 0 7 24 14 1302.2 0 14 25 14 1302.2 0 14 26 7 1302.2 0 7 27 15 1302.2 0 15 28 26 1302.2 0 26 29 166 1302.2 0 166 30 39 1302.2 0 39 31 10 1302.2 0 10 32 18 1302.2 0 18 33 5 1302.2 0 5 34 5 1302.2 0 5 35 12 1302.2 0 12 36 15 1302.2 0 15 37 8 1302.2 0 8 38 6 1302.2 0 6 39 12 1302.2 0 12 40 72 1302.2 0 72 41 88 1302.2 0 88 42 7 1302.2 0 7 43 15 1302.2 0 15 44 8 1302.2 0 8 45 50 1302.2 0 50 46 100 1302.2 0 100 47 90 1302.2 0 90 48 14 1302.2 0 14 49 8 1302.2 0 8 50 9 1302.2 0 9 51 77 1302.2 0 77 52 93 1302.2 0 93 53 18 1302.2 0 18 54 6 1302.2 0 6 55 8 1302.2 0 8 56 6 1302.2 0 6 57 9 1302.2 0 9 58 4 1302.2 0 4 59 8 1302.2 0 8 60 31 1302.2 0 31 61 28 1302.2 0 28 62 11 1302.2 0 11 63 8 1302.2 0 8 64 4 1302.2 0 4 65 10 1302.2 0 10 66 8 1302.2 0 8 67 10 1302.2 0 10 68 7 1302.2 0 7 69 10 1302.2 0 10 70 34 1302.2 0 34 71 249 1302.2 0 249 72 329 1302.2 0 329 73 46 1302.2 0 46 74 324 1302.2 0 324 75 180 1302.2 0 180 76 427 1302.2 0 427 77 1878 1302.2 0 1878 78 967 1302.2 0 967 79 885 1302.2 0 885 80 4374 1302.2 0 4374 81 1494 1302.2 0 1494 82 1525 1302.2 0 1525 83 4135 1302.2 0 4135 84 3892 1302.2 0 3892 85 411 1302.2 0 411 86 235 1302.2 0 235 87 674 1302.2 0 674 88 150 1302.2 0 150 89 150 1302.2 0 150 90 2103 1302.2 0 2103 91 728 1302.2 0 728 92 1391 1302.2 0 1391 93 4227 1302.2 0 4227 94 719 1302.2 0 719 95 1043 1302.2 0 1043 96 347 1302.2 0 347 97 842 1302.2 0 842 98 564 1302.2 0 564 99 373 1302.2 0 373 100 453 1302.2 0 453 101 224 1302.2 0 224 102 52 1302.2 0 52 103 76 1302.2 0 76 104 150 1302.2 0 150 105 57 1302.2 0 57 106 68 1302.2 0 68 107 240 1302.2 0 240 108 98 1302.2 0 98 109 144 1302.2 0 144 110 474 1302.2 0 474 111 342 1302.2 0 342 112 263 1302.2 0 263 113 1324 1302.2 0 1324 114 1551 1302.2 0 1551 115 2422 1302.2 0 2422 116 1373 1302.2 0 1373 117 535 1302.2 0 535 118 954 1302.2 0 954 119 677 1302.2 0 677 120 457 1302.2 0 457 121 455 1302.2 0 455 122 408 1302.2 0 408 123 550 1302.2 0 550 124 536 1302.2 0 536 125 519 1302.2 0 519 126 316 1302.2 0 316 127 261 1302.2 0 261 128 647 1302.2 0 647 129 853 1302.2 0 853 130 936 1302.2 0 936 131 1371 1302.2 0 1371 132 712 1302.2 0 712 133 607 1302.2 0 607 134 590 1302.2 0 590 135 1564 1302.2 0 1564 136 1159 1302.2 0 1159 137 1252 1302.2 0 1252 138 719 1302.2 0 719 139 603 1302.2 0 603 140 355 1302.2 0 355 141 383 1302.2 0 383 142 487 1302.2 0 487 143 744 1302.2 0 744 144 678 1302.2 0 678 145 651 1302.2 0 651 146 370 1302.2 0 370 147 303 1302.2 0 303 148 264 1302.2 0 264 149 291 1302.2 0 291 150 373 1302.2 0 373 151 347 1302.2 0 347 152 509 1302.2 0 509 153 426 1302.2 0 426 154 650 1302.2 0 650 155 794 1302.2 0 794 156 501 1302.2 0 501 157 683 1302.2 0 683 158 552 1302.2 0 552 159 444 1302.2 0 444 160 354 1302.2 0 354 161 397 1302.2 0 397 162 549 1302.2 0 549 163 682 1302.2 0 682 164 738 1302.2 0 738 165 662 1302.2 0 662 166 541 1302.2 0 541 167 660 1302.2 0 660 168 620 1302.2 0 620 169 416 1302.2 0 416 170 381 1302.2 0 381 171 632 1302.2 0 632 172 303 1302.2 0 303 173 424 1302.2 0 424 174 401 1302.2 0 401 175 279 1302.2 0 279 176 236 1302.2 0 236 177 539 1302.2 0 539 178 944 1302.2 0 944 179 1009 1302.2 0 1009 180 736 1302.2 0 736 181 453 1302.2 0 453 182 479 1302.2 0 479 183 512 1302.2 0 512 184 628 1302.2 0 628 185 479 1302.2 0 479 186 220 1302.2 0 220 187 225 1302.2 0 225 188 370 1302.2 0 370 189 740 1302.2 0 740 190 1056 1302.2 0 1056 191 899 1302.2 0 899 192 703 1302.2 0 703 193 301 1302.2 0 301 194 197 1302.2 0 197 195 474 1302.2 0 474 196 500 1302.2 0 500 197 519 1302.2 0 519 198 485 1302.2 0 485 199 285 1302.2 0 285 200 263 1302.2 0 263 201 452 1302.2 0 452 202 898 1302.2 0 898 203 1598 1302.2 0 1598 204 1132 1302.2 0 1132 205 481 1302.2 0 481 206 331 1302.2 0 331 207 409 1302.2 0 409 208 410 1302.2 0 410 209 902 1302.2 0 902 210 1258 1302.2 0 1258 211 977 1302.2 0 977 212 764 1302.2 0 764 213 469 1302.2 0 469 214 470 1302.2 0 470 215 879 1302.2 0 879 216 798 1302.2 0 798 217 766 1302.2 0 766 218 352 1302.2 0 352 219 203 1302.2 0 203 220 337 1302.2 0 337 221 448 1302.2 0 448 222 464 1302.2 0 464 223 655 1302.2 0 655 224 633 1302.2 0 633 225 729 1302.2 0 729 226 769 1302.2 0 769 227 949 1302.2 0 949 228 325 1302.2 0 325 229 332 1302.2 0 332 230 231 1302.2 0 231 231 387 1302.2 0 387 232 578 1302.2 0 578 233 562 1302.2 0 562 234 699 1302.2 0 699 235 998 1302.2 0 998 236 1001 1302.2 0 1001 237 980 1302.2 0 980 238 378 1302.2 0 378 239 298 1302.2 0 298 240 314 1302.2 0 314 241 571 1302.2 0 571 242 621 1302.2 0 621 243 476 1302.2 0 476 244 414 1302.2 0 414 245 224 1302.2 0 224 246 175 1302.2 0 175 247 161 1302.2 0 161 248 219 1302.2 0 219 249 308 1302.2 0 308 250 554 1302.2 0 554 251 474 1302.2 0 474 252 596 1302.2 0 596 253 525 1302.2 0 525 254 310 1302.2 0 310 255 158 1302.2 0 158 256 80 1302.2 0 80 257 145 1302.2 0 145 258 120 1302.2 0 120 259 226 1302.2 0 226 260 336 1302.2 0 336 261 431 1302.2 0 431 262 439 1302.2 0 439 263 706 1302.2 0 706 264 592 1302.2 0 592 265 676 1302.2 0 676 266 300 1302.2 0 300 267 220 1302.2 0 220 268 182 1302.2 0 182 269 191 1302.2 0 191 270 492 1302.2 0 492 271 549 1302.2 0 549 272 352 1302.2 0 352 273 306 1302.2 0 306 274 362 1302.2 0 362 275 298 1302.2 0 298 276 346 1302.2 0 346 277 454 1302.2 0 454 278 437 1302.2 0 437 279 268 1302.2 0 268 280 177 1302.2 0 177 281 151 1302.2 0 151 282 232 1302.2 0 232 283 284 1302.2 0 284 284 308 1302.2 0 308 285 238 1302.2 0 238 286 280 1302.2 0 280 287 176 1302.2 0 176 288 306 1302.2 0 306 289 655 1302.2 0 655 290 844 1302.2 0 844 291 652 1302.2 0 652 292 326 1302.2 0 326 293 258 1302.2 0 258 294 266 1302.2 0 266 295 354 1302.2 0 354 296 416 1302.2 0 416 297 516 1302.2 0 516 298 366 1302.2 0 366 299 352 1302.2 0 352 300 600 1302.2 0 600 301 407 1302.2 0 407 Finished in 4.51 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 97,890 Reads with adapters: 3,786 (3.9%) Reads that were too short: 53 (0.1%) Reads written (passing filters): 3,733 (3.8%) Total basepairs processed: 17,822,727 bp Total written (filtered): 1,011,144 bp (5.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3786 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 1 0.0 3 0 0 0 0 1 19 30 0.0 3 0 24 2 1 3 20 3649 0.0 4 3548 83 12 5 1 21 14 0.0 4 3 6 1 4 22 3 0.0 4 1 0 2 23 7 0.0 4 0 0 0 2 5 24 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 47 7 0.0 4 0 0 0 0 7 49 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 66 2 0.0 4 0 0 0 0 2 67 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 78 3 0.0 4 0 0 0 0 3 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 85 2 0.0 4 0 0 0 0 2 89 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 108 4 0.0 4 0 0 0 0 4 110 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 121 2 0.0 4 0 0 0 0 2 122 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 132 2 0.0 4 0 0 0 0 2 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 139 3 0.0 4 0 0 0 0 3 140 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 1 144 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 155 2 0.0 4 0 0 0 0 2 157 1 0.0 4 0 0 0 0 1 159 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 212 2 0.0 4 0 0 0 0 2 221 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 3,733 Reads with adapters: 243 (6.5%) Reads written (passing filters): 3,733 (100.0%) Total basepairs processed: 1,011,144 bp Total written (filtered): 1,008,640 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 243 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.2% C: 0.8% G: 92.2% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 2 14.6 0 2 8 1 0.1 0 1 9 47 0.0 0 1 46 10 62 0.0 1 2 60 11 128 0.0 1 1 127 12 2 0.0 1 0 2 13 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.