This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.05 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 785,124 (26.8%) Reads that were too short: 145,865 (5.0%) Reads written (passing filters): 639,259 (21.8%) Total basepairs processed: 881,011,047 bp Total written (filtered): 112,712,525 bp (12.8%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 785124 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 368 714.6 0 368 7 13509 714.6 0 13509 8 11115 714.6 0 11115 9 380 714.6 0 380 10 211 714.6 0 211 11 67 714.6 0 67 12 63 714.6 0 63 13 64 714.6 0 64 14 57 714.6 0 57 15 63 714.6 0 63 16 55 714.6 0 55 17 54 714.6 0 54 18 51 714.6 0 51 19 57 714.6 0 57 20 65 714.6 0 65 21 38 714.6 0 38 22 52 714.6 0 52 23 51 714.6 0 51 24 61 714.6 0 61 25 83 714.6 0 83 26 88 714.6 0 88 27 134 714.6 0 134 28 74 714.6 0 74 29 70 714.6 0 70 30 71 714.6 0 71 31 48 714.6 0 48 32 52 714.6 0 52 33 44 714.6 0 44 34 107 714.6 0 107 35 73 714.6 0 73 36 55 714.6 0 55 37 95 714.6 0 95 38 51 714.6 0 51 39 41 714.6 0 41 40 82 714.6 0 82 41 61 714.6 0 61 42 55 714.6 0 55 43 82 714.6 0 82 44 64 714.6 0 64 45 54 714.6 0 54 46 56 714.6 0 56 47 60 714.6 0 60 48 51 714.6 0 51 49 42 714.6 0 42 50 68 714.6 0 68 51 50 714.6 0 50 52 59 714.6 0 59 53 69 714.6 0 69 54 81 714.6 0 81 55 50 714.6 0 50 56 73 714.6 0 73 57 64 714.6 0 64 58 45 714.6 0 45 59 106 714.6 0 106 60 68 714.6 0 68 61 80 714.6 0 80 62 75 714.6 0 75 63 83 714.6 0 83 64 82 714.6 0 82 65 82 714.6 0 82 66 93 714.6 0 93 67 117 714.6 0 117 68 145 714.6 0 145 69 158 714.6 0 158 70 193 714.6 0 193 71 306 714.6 0 306 72 2021 714.6 0 2021 73 1368 714.6 0 1368 74 2884 714.6 0 2884 75 5421 714.6 0 5421 76 2267 714.6 0 2267 77 3491 714.6 0 3491 78 4470 714.6 0 4470 79 7050 714.6 0 7050 80 5889 714.6 0 5889 81 8997 714.6 0 8997 82 6343 714.6 0 6343 83 8876 714.6 0 8876 84 11735 714.6 0 11735 85 9329 714.6 0 9329 86 5974 714.6 0 5974 87 7289 714.6 0 7289 88 3398 714.6 0 3398 89 2536 714.6 0 2536 90 6139 714.6 0 6139 91 2242 714.6 0 2242 92 3043 714.6 0 3043 93 5490 714.6 0 5490 94 3695 714.6 0 3695 95 3291 714.6 0 3291 96 3791 714.6 0 3791 97 2699 714.6 0 2699 98 3915 714.6 0 3915 99 3067 714.6 0 3067 100 2010 714.6 0 2010 101 3350 714.6 0 3350 102 2561 714.6 0 2561 103 1830 714.6 0 1830 104 1467 714.6 0 1467 105 1866 714.6 0 1866 106 1654 714.6 0 1654 107 1392 714.6 0 1392 108 1998 714.6 0 1998 109 2122 714.6 0 2122 110 2643 714.6 0 2643 111 3060 714.6 0 3060 112 2718 714.6 0 2718 113 3384 714.6 0 3384 114 3752 714.6 0 3752 115 3825 714.6 0 3825 116 4010 714.6 0 4010 117 5125 714.6 0 5125 118 12553 714.6 0 12553 119 40185 714.6 0 40185 120 17584 714.6 0 17584 121 4606 714.6 0 4606 122 5888 714.6 0 5888 123 4930 714.6 0 4930 124 2781 714.6 0 2781 125 2434 714.6 0 2434 126 3082 714.6 0 3082 127 3094 714.6 0 3094 128 2407 714.6 0 2407 129 3232 714.6 0 3232 130 3046 714.6 0 3046 131 2793 714.6 0 2793 132 3689 714.6 0 3689 133 3038 714.6 0 3038 134 3214 714.6 0 3214 135 3285 714.6 0 3285 136 3109 714.6 0 3109 137 3438 714.6 0 3438 138 3466 714.6 0 3466 139 3919 714.6 0 3919 140 13836 714.6 0 13836 141 10573 714.6 0 10573 142 10214 714.6 0 10214 143 4693 714.6 0 4693 144 4507 714.6 0 4507 145 10323 714.6 0 10323 146 38284 714.6 0 38284 147 23748 714.6 0 23748 148 7456 714.6 0 7456 149 34460 714.6 0 34460 150 5512 714.6 0 5512 151 3144 714.6 0 3144 152 4680 714.6 0 4680 153 4200 714.6 0 4200 154 2351 714.6 0 2351 155 2781 714.6 0 2781 156 2404 714.6 0 2404 157 2048 714.6 0 2048 158 2401 714.6 0 2401 159 1928 714.6 0 1928 160 1999 714.6 0 1999 161 1741 714.6 0 1741 162 2093 714.6 0 2093 163 1860 714.6 0 1860 164 2120 714.6 0 2120 165 3524 714.6 0 3524 166 6990 714.6 0 6990 167 6692 714.6 0 6692 168 2317 714.6 0 2317 169 2238 714.6 0 2238 170 2025 714.6 0 2025 171 1914 714.6 0 1914 172 2294 714.6 0 2294 173 2333 714.6 0 2333 174 1951 714.6 0 1951 175 2308 714.6 0 2308 176 2168 714.6 0 2168 177 1737 714.6 0 1737 178 2078 714.6 0 2078 179 1661 714.6 0 1661 180 1694 714.6 0 1694 181 1962 714.6 0 1962 182 1754 714.6 0 1754 183 1782 714.6 0 1782 184 2002 714.6 0 2002 185 2211 714.6 0 2211 186 2094 714.6 0 2094 187 1873 714.6 0 1873 188 2249 714.6 0 2249 189 2027 714.6 0 2027 190 1832 714.6 0 1832 191 1995 714.6 0 1995 192 2365 714.6 0 2365 193 2882 714.6 0 2882 194 1969 714.6 0 1969 195 2617 714.6 0 2617 196 2801 714.6 0 2801 197 1673 714.6 0 1673 198 2394 714.6 0 2394 199 1683 714.6 0 1683 200 2041 714.6 0 2041 201 2302 714.6 0 2302 202 1649 714.6 0 1649 203 1753 714.6 0 1753 204 1689 714.6 0 1689 205 1735 714.6 0 1735 206 1667 714.6 0 1667 207 1559 714.6 0 1559 208 1708 714.6 0 1708 209 1907 714.6 0 1907 210 1673 714.6 0 1673 211 1554 714.6 0 1554 212 1486 714.6 0 1486 213 1440 714.6 0 1440 214 1418 714.6 0 1418 215 1361 714.6 0 1361 216 1759 714.6 0 1759 217 2147 714.6 0 2147 218 1731 714.6 0 1731 219 1580 714.6 0 1580 220 1465 714.6 0 1465 221 1595 714.6 0 1595 222 1212 714.6 0 1212 223 1168 714.6 0 1168 224 1188 714.6 0 1188 225 1081 714.6 0 1081 226 1159 714.6 0 1159 227 1263 714.6 0 1263 228 1130 714.6 0 1130 229 1387 714.6 0 1387 230 1486 714.6 0 1486 231 1422 714.6 0 1422 232 1367 714.6 0 1367 233 1406 714.6 0 1406 234 1311 714.6 0 1311 235 1256 714.6 0 1256 236 1331 714.6 0 1331 237 1947 714.6 0 1947 238 1767 714.6 0 1767 239 1736 714.6 0 1736 240 2084 714.6 0 2084 241 1470 714.6 0 1470 242 1263 714.6 0 1263 243 1034 714.6 0 1034 244 1223 714.6 0 1223 245 1135 714.6 0 1135 246 1170 714.6 0 1170 247 1477 714.6 0 1477 248 2139 714.6 0 2139 249 1604 714.6 0 1604 250 1820 714.6 0 1820 251 1329 714.6 0 1329 252 984 714.6 0 984 253 898 714.6 0 898 254 729 714.6 0 729 255 786 714.6 0 786 256 863 714.6 0 863 257 1026 714.6 0 1026 258 1051 714.6 0 1051 259 1325 714.6 0 1325 260 1593 714.6 0 1593 261 1629 714.6 0 1629 262 1400 714.6 0 1400 263 1362 714.6 0 1362 264 1028 714.6 0 1028 265 892 714.6 0 892 266 1055 714.6 0 1055 267 1000 714.6 0 1000 268 1170 714.6 0 1170 269 1241 714.6 0 1241 270 1560 714.6 0 1560 271 1475 714.6 0 1475 272 1184 714.6 0 1184 273 1647 714.6 0 1647 274 1296 714.6 0 1296 275 1398 714.6 0 1398 276 1461 714.6 0 1461 277 1166 714.6 0 1166 278 1117 714.6 0 1117 279 1210 714.6 0 1210 280 1083 714.6 0 1083 281 1066 714.6 0 1066 282 1276 714.6 0 1276 283 964 714.6 0 964 284 1062 714.6 0 1062 285 1208 714.6 0 1208 286 964 714.6 0 964 287 1443 714.6 0 1443 288 1845 714.6 0 1845 289 1559 714.6 0 1559 290 1826 714.6 0 1826 291 1876 714.6 0 1876 292 1535 714.6 0 1535 293 1714 714.6 0 1714 294 1916 714.6 0 1916 295 1918 714.6 0 1918 296 2077 714.6 0 2077 297 2475 714.6 0 2475 298 2624 714.6 0 2624 299 2280 714.6 0 2280 300 1612 714.6 0 1612 301 2755 714.6 0 2755 Finished in 22.80 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 639,259 Reads with adapters: 11,652 (1.8%) Reads that were too short: 279 (0.0%) Reads written (passing filters): 11,373 (1.8%) Total basepairs processed: 112,712,525 bp Total written (filtered): 3,088,209 bp (2.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11652 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 2 1 19 107 0.0 3 0 97 4 3 3 20 11082 0.0 4 10756 278 28 14 6 21 36 0.0 4 3 32 0 1 22 9 0.0 4 4 0 2 1 2 23 10 0.0 4 0 0 0 6 4 24 6 0.0 4 0 0 0 0 6 25 2 0.0 4 0 0 0 0 2 26 2 0.0 4 0 0 0 0 2 27 4 0.0 4 0 0 0 2 2 29 2 0.0 4 0 0 0 0 2 30 1 0.0 4 0 0 0 0 1 31 3 0.0 4 0 0 0 0 3 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 5 0.0 4 0 0 0 0 5 39 4 0.0 4 0 0 0 0 4 40 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 42 5 0.0 4 0 0 0 0 5 43 2 0.0 4 0 0 0 0 2 45 1 0.0 4 0 0 0 0 1 46 4 0.0 4 0 0 0 0 4 47 2 0.0 4 0 0 0 0 2 48 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 50 19 0.0 4 0 0 0 0 19 52 3 0.0 4 0 0 0 0 3 53 4 0.0 4 0 0 0 1 3 54 2 0.0 4 0 0 0 0 2 57 2 0.0 4 0 0 0 1 1 58 1 0.0 4 0 0 0 0 1 59 2 0.0 4 0 0 0 0 2 60 3 0.0 4 0 0 0 0 3 61 3 0.0 4 0 0 0 0 3 62 2 0.0 4 0 0 0 0 2 63 3 0.0 4 0 0 0 0 3 64 2 0.0 4 0 0 0 0 2 65 1 0.0 4 0 0 0 0 1 66 4 0.0 4 0 1 0 0 3 67 4 0.0 4 0 0 0 0 4 68 1 0.0 4 0 0 0 0 1 70 2 0.0 4 0 0 0 0 2 72 2 0.0 4 0 0 0 0 2 73 1 0.0 4 0 0 0 0 1 74 2 0.0 4 0 0 0 0 2 75 5 0.0 4 0 0 0 0 5 76 4 0.0 4 0 0 0 1 3 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 5 0.0 4 0 0 0 0 5 80 2 0.0 4 0 0 0 0 2 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 3 0.0 4 0 0 0 0 3 85 8 0.0 4 0 0 0 0 8 86 6 0.0 4 0 0 0 0 6 87 5 0.0 4 0 0 0 0 5 88 4 0.0 4 0 0 0 0 4 89 6 0.0 4 0 0 0 0 6 90 1 0.0 4 0 0 0 0 1 91 6 0.0 4 0 0 0 0 6 92 1 0.0 4 0 0 0 0 1 93 3 0.0 4 0 0 0 0 3 94 3 0.0 4 0 0 0 0 3 95 2 0.0 4 0 0 0 0 2 96 5 0.0 4 0 0 0 0 5 97 1 0.0 4 0 0 0 0 1 98 2 0.0 4 0 0 0 0 2 99 4 0.0 4 0 0 0 0 4 100 5 0.0 4 0 0 0 0 5 101 2 0.0 4 0 0 0 0 2 102 3 0.0 4 0 0 0 0 3 103 1 0.0 4 0 0 0 0 1 104 3 0.0 4 0 0 0 0 3 106 3 0.0 4 0 0 0 0 3 107 3 0.0 4 0 0 0 0 3 108 2 0.0 4 0 0 0 0 2 109 2 0.0 4 0 0 0 0 2 110 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 1 1 112 4 0.0 4 0 0 0 0 4 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 5 0.0 4 0 0 0 0 5 117 3 0.0 4 0 0 0 0 3 118 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 0 2 120 2 0.0 4 0 0 0 0 2 121 2 0.0 4 0 0 0 0 2 122 2 0.0 4 0 0 0 0 2 123 5 0.0 4 0 0 0 0 5 124 13 0.0 4 0 0 0 0 13 125 5 0.0 4 0 0 0 0 5 126 2 0.0 4 0 0 0 0 2 127 8 0.0 4 0 0 0 0 8 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 131 2 0.0 4 0 0 0 0 2 132 3 0.0 4 0 0 0 0 3 133 3 0.0 4 0 0 0 0 3 134 4 0.0 4 0 0 0 1 3 135 1 0.0 4 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 4 0.0 4 0 0 0 0 4 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 2 0.0 4 0 0 0 0 2 142 4 0.0 4 0 0 0 0 4 143 3 0.0 4 0 0 0 0 3 144 3 0.0 4 0 0 0 0 3 145 1 0.0 4 0 0 0 0 1 146 6 0.0 4 0 0 0 0 6 147 1 0.0 4 0 0 0 0 1 148 3 0.0 4 0 0 0 0 3 149 2 0.0 4 0 0 0 0 2 151 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 155 3 0.0 4 0 0 0 0 3 156 8 0.0 4 0 0 0 0 8 157 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 161 2 0.0 4 0 0 0 0 2 162 3 0.0 4 0 0 0 0 3 163 1 0.0 4 0 0 0 0 1 164 3 0.0 4 0 0 0 0 3 165 1 0.0 4 0 0 0 1 166 3 0.0 4 0 0 0 0 3 168 2 0.0 4 0 0 0 0 2 169 1 0.0 4 0 0 0 0 1 170 2 0.0 4 0 0 0 1 1 171 3 0.0 4 0 0 0 0 3 173 2 0.0 4 0 0 0 0 2 175 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 180 4 0.0 4 0 0 0 0 4 181 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 185 2 0.0 4 0 0 0 1 1 186 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 189 3 0.0 4 0 0 0 0 3 191 2 0.0 4 0 1 0 0 1 198 2 0.0 4 0 0 0 0 2 201 1 0.0 4 0 0 0 0 1 205 3 0.0 4 0 0 0 0 3 209 1 0.0 4 0 0 0 0 1 210 2 0.0 4 0 0 0 1 1 213 2 0.0 4 0 0 0 0 2 218 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 11,373 Reads with adapters: 1,117 (9.8%) Reads written (passing filters): 11,373 (100.0%) Total basepairs processed: 3,088,209 bp Total written (filtered): 3,075,665 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1117 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 2.9% G: 92.7% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 177.7 0 1 9 6 0.0 0 0 6 10 38 0.0 1 3 35 11 1062 0.0 1 6 1056 12 8 0.0 1 1 7 151 1 0.0 1 1 178 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.