This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.24 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 261,846 (8.9%) Reads that were too short: 118,855 (4.1%) Reads written (passing filters): 142,991 (4.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 24,319,984 bp (2.8%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 261846 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 583 714.6 0 583 7 12721 714.6 0 12721 8 22 714.6 0 22 9 15 714.6 0 15 10 12 714.6 0 12 11 11 714.6 0 11 12 15 714.6 0 15 13 14 714.6 0 14 14 14 714.6 0 14 15 14 714.6 0 14 16 21 714.6 0 21 17 16 714.6 0 16 18 19 714.6 0 19 19 9 714.6 0 9 20 19 714.6 0 19 21 7 714.6 0 7 22 12 714.6 0 12 23 13 714.6 0 13 24 15 714.6 0 15 25 11 714.6 0 11 26 11 714.6 0 11 27 5 714.6 0 5 28 15 714.6 0 15 29 10 714.6 0 10 30 11 714.6 0 11 31 14 714.6 0 14 32 12 714.6 0 12 33 8 714.6 0 8 34 12 714.6 0 12 35 13 714.6 0 13 36 10 714.6 0 10 37 15 714.6 0 15 38 13 714.6 0 13 39 14 714.6 0 14 40 232 714.6 0 232 41 30 714.6 0 30 42 16 714.6 0 16 43 9 714.6 0 9 44 19 714.6 0 19 45 3 714.6 0 3 46 11 714.6 0 11 47 13 714.6 0 13 48 15 714.6 0 15 49 14 714.6 0 14 50 8 714.6 0 8 51 9 714.6 0 9 52 11 714.6 0 11 53 9 714.6 0 9 54 11 714.6 0 11 55 11 714.6 0 11 56 9 714.6 0 9 57 5 714.6 0 5 58 21 714.6 0 21 59 14 714.6 0 14 60 7 714.6 0 7 61 12 714.6 0 12 62 11 714.6 0 11 63 21 714.6 0 21 64 23 714.6 0 23 65 19 714.6 0 19 66 18 714.6 0 18 67 14 714.6 0 14 68 13 714.6 0 13 69 14 714.6 0 14 70 9 714.6 0 9 71 19 714.6 0 19 72 13 714.6 0 13 73 10 714.6 0 10 74 7 714.6 0 7 75 21 714.6 0 21 76 14 714.6 0 14 77 10 714.6 0 10 78 37 714.6 0 37 79 80 714.6 0 80 80 136 714.6 0 136 81 175 714.6 0 175 82 234 714.6 0 234 83 337 714.6 0 337 84 389 714.6 0 389 85 628 714.6 0 628 86 459 714.6 0 459 87 320 714.6 0 320 88 627 714.6 0 627 89 217 714.6 0 217 90 208 714.6 0 208 91 329 714.6 0 329 92 259 714.6 0 259 93 414 714.6 0 414 94 597 714.6 0 597 95 1051 714.6 0 1051 96 2001 714.6 0 2001 97 1173 714.6 0 1173 98 1111 714.6 0 1111 99 1176 714.6 0 1176 100 677 714.6 0 677 101 816 714.6 0 816 102 828 714.6 0 828 103 786 714.6 0 786 104 931 714.6 0 931 105 978 714.6 0 978 106 1373 714.6 0 1373 107 949 714.6 0 949 108 1363 714.6 0 1363 109 1407 714.6 0 1407 110 1844 714.6 0 1844 111 789 714.6 0 789 112 1468 714.6 0 1468 113 1470 714.6 0 1470 114 2590 714.6 0 2590 115 3098 714.6 0 3098 116 1573 714.6 0 1573 117 837 714.6 0 837 118 504 714.6 0 504 119 339 714.6 0 339 120 369 714.6 0 369 121 702 714.6 0 702 122 1353 714.6 0 1353 123 1187 714.6 0 1187 124 872 714.6 0 872 125 556 714.6 0 556 126 862 714.6 0 862 127 997 714.6 0 997 128 886 714.6 0 886 129 1481 714.6 0 1481 130 2426 714.6 0 2426 131 1285 714.6 0 1285 132 1157 714.6 0 1157 133 857 714.6 0 857 134 696 714.6 0 696 135 1029 714.6 0 1029 136 1211 714.6 0 1211 137 2174 714.6 0 2174 138 1330 714.6 0 1330 139 912 714.6 0 912 140 818 714.6 0 818 141 1052 714.6 0 1052 142 1387 714.6 0 1387 143 999 714.6 0 999 144 897 714.6 0 897 145 730 714.6 0 730 146 941 714.6 0 941 147 1005 714.6 0 1005 148 1789 714.6 0 1789 149 1394 714.6 0 1394 150 1180 714.6 0 1180 151 1060 714.6 0 1060 152 892 714.6 0 892 153 1043 714.6 0 1043 154 1423 714.6 0 1423 155 1550 714.6 0 1550 156 1884 714.6 0 1884 157 1484 714.6 0 1484 158 1894 714.6 0 1894 159 1040 714.6 0 1040 160 799 714.6 0 799 161 1030 714.6 0 1030 162 980 714.6 0 980 163 1826 714.6 0 1826 164 1510 714.6 0 1510 165 1252 714.6 0 1252 166 1338 714.6 0 1338 167 1152 714.6 0 1152 168 1129 714.6 0 1129 169 909 714.6 0 909 170 787 714.6 0 787 171 762 714.6 0 762 172 639 714.6 0 639 173 590 714.6 0 590 174 817 714.6 0 817 175 1110 714.6 0 1110 176 1424 714.6 0 1424 177 1024 714.6 0 1024 178 929 714.6 0 929 179 873 714.6 0 873 180 908 714.6 0 908 181 1147 714.6 0 1147 182 1134 714.6 0 1134 183 1113 714.6 0 1113 184 1058 714.6 0 1058 185 1070 714.6 0 1070 186 1066 714.6 0 1066 187 1112 714.6 0 1112 188 927 714.6 0 927 189 752 714.6 0 752 190 724 714.6 0 724 191 777 714.6 0 777 192 1198 714.6 0 1198 193 1534 714.6 0 1534 194 1470 714.6 0 1470 195 950 714.6 0 950 196 1387 714.6 0 1387 197 708 714.6 0 708 198 900 714.6 0 900 199 1197 714.6 0 1197 200 1376 714.6 0 1376 201 1770 714.6 0 1770 202 1332 714.6 0 1332 203 932 714.6 0 932 204 1087 714.6 0 1087 205 1051 714.6 0 1051 206 1055 714.6 0 1055 207 974 714.6 0 974 208 1212 714.6 0 1212 209 2074 714.6 0 2074 210 1811 714.6 0 1811 211 1273 714.6 0 1273 212 704 714.6 0 704 213 773 714.6 0 773 214 846 714.6 0 846 215 899 714.6 0 899 216 877 714.6 0 877 217 994 714.6 0 994 218 1103 714.6 0 1103 219 1059 714.6 0 1059 220 905 714.6 0 905 221 1259 714.6 0 1259 222 1551 714.6 0 1551 223 1214 714.6 0 1214 224 1021 714.6 0 1021 225 846 714.6 0 846 226 958 714.6 0 958 227 941 714.6 0 941 228 1060 714.6 0 1060 229 1018 714.6 0 1018 230 1227 714.6 0 1227 231 962 714.6 0 962 232 973 714.6 0 973 233 842 714.6 0 842 234 868 714.6 0 868 235 1029 714.6 0 1029 236 1333 714.6 0 1333 237 1357 714.6 0 1357 238 1492 714.6 0 1492 239 1111 714.6 0 1111 240 1110 714.6 0 1110 241 1172 714.6 0 1172 242 1297 714.6 0 1297 243 1248 714.6 0 1248 244 1799 714.6 0 1799 245 2133 714.6 0 2133 246 2223 714.6 0 2223 247 1858 714.6 0 1858 248 2018 714.6 0 2018 249 1400 714.6 0 1400 250 577 714.6 0 577 251 519 714.6 0 519 252 282 714.6 0 282 253 880 714.6 0 880 254 1460 714.6 0 1460 255 1448 714.6 0 1448 256 1243 714.6 0 1243 257 1364 714.6 0 1364 258 1705 714.6 0 1705 259 1451 714.6 0 1451 260 1088 714.6 0 1088 261 814 714.6 0 814 262 713 714.6 0 713 263 1233 714.6 0 1233 264 1073 714.6 0 1073 265 1242 714.6 0 1242 266 1038 714.6 0 1038 267 1508 714.6 0 1508 268 1312 714.6 0 1312 269 1854 714.6 0 1854 270 1204 714.6 0 1204 271 1061 714.6 0 1061 272 900 714.6 0 900 273 1029 714.6 0 1029 274 1374 714.6 0 1374 275 1011 714.6 0 1011 276 1034 714.6 0 1034 277 887 714.6 0 887 278 775 714.6 0 775 279 1167 714.6 0 1167 280 1375 714.6 0 1375 281 1145 714.6 0 1145 282 1567 714.6 0 1567 283 924 714.6 0 924 284 1001 714.6 0 1001 285 1180 714.6 0 1180 286 1483 714.6 0 1483 287 1519 714.6 0 1519 288 1300 714.6 0 1300 289 963 714.6 0 963 290 932 714.6 0 932 291 732 714.6 0 732 292 1068 714.6 0 1068 293 1509 714.6 0 1509 294 1694 714.6 0 1694 295 2028 714.6 0 2028 296 1764 714.6 0 1764 297 1054 714.6 0 1054 298 958 714.6 0 958 299 819 714.6 0 819 300 679 714.6 0 679 301 1634 714.6 0 1634 Finished in 4.63 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 142,991 Reads with adapters: 13,296 (9.3%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 13,273 (9.3%) Total basepairs processed: 24,319,984 bp Total written (filtered): 3,669,249 bp (15.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13296 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 76 0.0 3 0 75 1 17 10535 0.0 3 10409 121 2 3 18 49 0.0 3 1 45 3 19 2301 0.0 3 2242 45 12 2 20 294 0.0 3 274 8 2 10 21 2 0.0 3 0 0 2 22 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 1 1 188 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 13,273 Reads with adapters: 857 (6.5%) Reads written (passing filters): 13,273 (100.0%) Total basepairs processed: 3,669,249 bp Total written (filtered): 3,666,677 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 857 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.7% C: 67.8% G: 16.5% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 856 207.4 0 856 4 1 51.8 0 1