This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.32 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 62,115 (2.1%) Reads that were too short: 10,420 (0.4%) Reads written (passing filters): 51,695 (1.8%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,365,085 bp (0.9%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 62115 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 98 714.6 0 98 7 1695 714.6 0 1695 8 143 714.6 0 143 9 4449 714.6 0 4449 10 37 714.6 0 37 11 30 714.6 0 30 12 44 714.6 0 44 13 25 714.6 0 25 14 41 714.6 0 41 15 29 714.6 0 29 16 33 714.6 0 33 17 34 714.6 0 34 18 28 714.6 0 28 19 86 714.6 0 86 20 23 714.6 0 23 21 30 714.6 0 30 22 42 714.6 0 42 23 34 714.6 0 34 24 25 714.6 0 25 25 17 714.6 0 17 26 25 714.6 0 25 27 21 714.6 0 21 28 39 714.6 0 39 29 39 714.6 0 39 30 22 714.6 0 22 31 28 714.6 0 28 32 49 714.6 0 49 33 138 714.6 0 138 34 148 714.6 0 148 35 105 714.6 0 105 36 19 714.6 0 19 37 23 714.6 0 23 38 23 714.6 0 23 39 18 714.6 0 18 40 23 714.6 0 23 41 22 714.6 0 22 42 16 714.6 0 16 43 34 714.6 0 34 44 31 714.6 0 31 45 70 714.6 0 70 46 35 714.6 0 35 47 33 714.6 0 33 48 14 714.6 0 14 49 36 714.6 0 36 50 31 714.6 0 31 51 34 714.6 0 34 52 22 714.6 0 22 53 33 714.6 0 33 54 26 714.6 0 26 55 23 714.6 0 23 56 33 714.6 0 33 57 87 714.6 0 87 58 35 714.6 0 35 59 39 714.6 0 39 60 49 714.6 0 49 61 21 714.6 0 21 62 19 714.6 0 19 63 32 714.6 0 32 64 20 714.6 0 20 65 28 714.6 0 28 66 20 714.6 0 20 67 31 714.6 0 31 68 31 714.6 0 31 69 18 714.6 0 18 70 24 714.6 0 24 71 22 714.6 0 22 72 125 714.6 0 125 73 37 714.6 0 37 74 38 714.6 0 38 75 32 714.6 0 32 76 22 714.6 0 22 77 33 714.6 0 33 78 35 714.6 0 35 79 32 714.6 0 32 80 23 714.6 0 23 81 26 714.6 0 26 82 32 714.6 0 32 83 28 714.6 0 28 84 146 714.6 0 146 85 100 714.6 0 100 86 68 714.6 0 68 87 68 714.6 0 68 88 113 714.6 0 113 89 46 714.6 0 46 90 54 714.6 0 54 91 65 714.6 0 65 92 59 714.6 0 59 93 81 714.6 0 81 94 101 714.6 0 101 95 62 714.6 0 62 96 73 714.6 0 73 97 119 714.6 0 119 98 89 714.6 0 89 99 67 714.6 0 67 100 62 714.6 0 62 101 31 714.6 0 31 102 50 714.6 0 50 103 63 714.6 0 63 104 43 714.6 0 43 105 75 714.6 0 75 106 458 714.6 0 458 107 1502 714.6 0 1502 108 1701 714.6 0 1701 109 1200 714.6 0 1200 110 41 714.6 0 41 111 55 714.6 0 55 112 37 714.6 0 37 113 22 714.6 0 22 114 46 714.6 0 46 115 63 714.6 0 63 116 56 714.6 0 56 117 52 714.6 0 52 118 35 714.6 0 35 119 43 714.6 0 43 120 45 714.6 0 45 121 149 714.6 0 149 122 96 714.6 0 96 123 59 714.6 0 59 124 46 714.6 0 46 125 44 714.6 0 44 126 55 714.6 0 55 127 149 714.6 0 149 128 455 714.6 0 455 129 500 714.6 0 500 130 121 714.6 0 121 131 103 714.6 0 103 132 51 714.6 0 51 133 49 714.6 0 49 134 89 714.6 0 89 135 115 714.6 0 115 136 312 714.6 0 312 137 276 714.6 0 276 138 137 714.6 0 137 139 74 714.6 0 74 140 98 714.6 0 98 141 177 714.6 0 177 142 161 714.6 0 161 143 302 714.6 0 302 144 726 714.6 0 726 145 393 714.6 0 393 146 104 714.6 0 104 147 157 714.6 0 157 148 62 714.6 0 62 149 80 714.6 0 80 150 75 714.6 0 75 151 61 714.6 0 61 152 103 714.6 0 103 153 375 714.6 0 375 154 351 714.6 0 351 155 290 714.6 0 290 156 201 714.6 0 201 157 112 714.6 0 112 158 85 714.6 0 85 159 97 714.6 0 97 160 83 714.6 0 83 161 102 714.6 0 102 162 102 714.6 0 102 163 65 714.6 0 65 164 50 714.6 0 50 165 90 714.6 0 90 166 116 714.6 0 116 167 116 714.6 0 116 168 101 714.6 0 101 169 188 714.6 0 188 170 186 714.6 0 186 171 87 714.6 0 87 172 57 714.6 0 57 173 73 714.6 0 73 174 151 714.6 0 151 175 190 714.6 0 190 176 147 714.6 0 147 177 86 714.6 0 86 178 78 714.6 0 78 179 145 714.6 0 145 180 112 714.6 0 112 181 212 714.6 0 212 182 238 714.6 0 238 183 348 714.6 0 348 184 381 714.6 0 381 185 843 714.6 0 843 186 19635 714.6 0 19635 187 2520 714.6 0 2520 188 1460 714.6 0 1460 189 64 714.6 0 64 190 44 714.6 0 44 191 87 714.6 0 87 192 71 714.6 0 71 193 62 714.6 0 62 194 67 714.6 0 67 195 54 714.6 0 54 196 38 714.6 0 38 197 46 714.6 0 46 198 42 714.6 0 42 199 65 714.6 0 65 200 66 714.6 0 66 201 51 714.6 0 51 202 67 714.6 0 67 203 100 714.6 0 100 204 256 714.6 0 256 205 251 714.6 0 251 206 105 714.6 0 105 207 96 714.6 0 96 208 130 714.6 0 130 209 106 714.6 0 106 210 85 714.6 0 85 211 104 714.6 0 104 212 72 714.6 0 72 213 87 714.6 0 87 214 92 714.6 0 92 215 97 714.6 0 97 216 117 714.6 0 117 217 642 714.6 0 642 218 1311 714.6 0 1311 219 406 714.6 0 406 220 58 714.6 0 58 221 84 714.6 0 84 222 85 714.6 0 85 223 69 714.6 0 69 224 59 714.6 0 59 225 63 714.6 0 63 226 87 714.6 0 87 227 173 714.6 0 173 228 89 714.6 0 89 229 68 714.6 0 68 230 49 714.6 0 49 231 60 714.6 0 60 232 84 714.6 0 84 233 62 714.6 0 62 234 108 714.6 0 108 235 74 714.6 0 74 236 68 714.6 0 68 237 70 714.6 0 70 238 61 714.6 0 61 239 46 714.6 0 46 240 31 714.6 0 31 241 35 714.6 0 35 242 63 714.6 0 63 243 89 714.6 0 89 244 134 714.6 0 134 245 284 714.6 0 284 246 89 714.6 0 89 247 47 714.6 0 47 248 44 714.6 0 44 249 21 714.6 0 21 250 42 714.6 0 42 251 94 714.6 0 94 252 137 714.6 0 137 253 216 714.6 0 216 254 170 714.6 0 170 255 124 714.6 0 124 256 84 714.6 0 84 257 82 714.6 0 82 258 62 714.6 0 62 259 36 714.6 0 36 260 38 714.6 0 38 261 36 714.6 0 36 262 95 714.6 0 95 263 100 714.6 0 100 264 83 714.6 0 83 265 72 714.6 0 72 266 87 714.6 0 87 267 81 714.6 0 81 268 65 714.6 0 65 269 39 714.6 0 39 270 47 714.6 0 47 271 53 714.6 0 53 272 100 714.6 0 100 273 59 714.6 0 59 274 32 714.6 0 32 275 40 714.6 0 40 276 33 714.6 0 33 277 82 714.6 0 82 278 141 714.6 0 141 279 115 714.6 0 115 280 212 714.6 0 212 281 111 714.6 0 111 282 98 714.6 0 98 283 36 714.6 0 36 284 63 714.6 0 63 285 138 714.6 0 138 286 64 714.6 0 64 287 39 714.6 0 39 288 29 714.6 0 29 289 32 714.6 0 32 290 30 714.6 0 30 291 57 714.6 0 57 292 49 714.6 0 49 293 34 714.6 0 34 294 37 714.6 0 37 295 42 714.6 0 42 296 57 714.6 0 57 297 109 714.6 0 109 298 149 714.6 0 149 299 73 714.6 0 73 300 23 714.6 0 23 301 15 714.6 0 15 Finished in 1.85 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 51,695 Reads with adapters: 1,746 (3.4%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 1,722 (3.3%) Total basepairs processed: 8,365,085 bp Total written (filtered): 464,914 bp (5.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1746 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 19 17 0.0 3 0 16 0 1 20 1631 0.0 4 1584 43 3 1 21 5 0.0 4 2 3 22 1 0.0 4 0 1 23 3 0.0 4 0 0 0 3 51 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 106 2 0.0 4 0 0 0 0 2 111 2 0.0 4 0 0 0 0 2 127 61 0.0 4 0 0 0 0 61 134 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 238 11 0.0 4 0 0 0 0 11 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (29 us/read; 2.07 M reads/minute). === Summary === Total reads processed: 1,722 Reads with adapters: 52 (3.0%) Reads written (passing filters): 1,722 (100.0%) Total basepairs processed: 464,914 bp Total written (filtered): 464,022 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 52 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.8% C: 5.8% G: 88.5% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 26.9 0 3 11 43 0.0 1 1 42 12 4 0.0 1 1 3 152 1 0.0 1 1 210 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.