This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.82 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 18,565 (0.6%) Reads that were too short: 4,450 (0.2%) Reads written (passing filters): 14,115 (0.5%) Total basepairs processed: 881,011,047 bp Total written (filtered): 3,061,881 bp (0.3%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 18565 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 76 714.6 0 76 7 72 714.6 0 72 8 117 714.6 0 117 9 1835 714.6 0 1835 10 76 714.6 0 76 11 60 714.6 0 60 12 62 714.6 0 62 13 78 714.6 0 78 14 66 714.6 0 66 15 53 714.6 0 53 16 42 714.6 0 42 17 54 714.6 0 54 18 82 714.6 0 82 19 58 714.6 0 58 20 56 714.6 0 56 21 60 714.6 0 60 22 47 714.6 0 47 23 45 714.6 0 45 24 49 714.6 0 49 25 67 714.6 0 67 26 58 714.6 0 58 27 55 714.6 0 55 28 64 714.6 0 64 29 48 714.6 0 48 30 46 714.6 0 46 31 52 714.6 0 52 32 44 714.6 0 44 33 66 714.6 0 66 34 101 714.6 0 101 35 61 714.6 0 61 36 51 714.6 0 51 37 62 714.6 0 62 38 65 714.6 0 65 39 64 714.6 0 64 40 58 714.6 0 58 41 72 714.6 0 72 42 59 714.6 0 59 43 56 714.6 0 56 44 45 714.6 0 45 45 51 714.6 0 51 46 68 714.6 0 68 47 141 714.6 0 141 48 92 714.6 0 92 49 111 714.6 0 111 50 61 714.6 0 61 51 301 714.6 0 301 52 728 714.6 0 728 53 63 714.6 0 63 54 66 714.6 0 66 55 67 714.6 0 67 56 45 714.6 0 45 57 40 714.6 0 40 58 57 714.6 0 57 59 66 714.6 0 66 60 108 714.6 0 108 61 119 714.6 0 119 62 81 714.6 0 81 63 56 714.6 0 56 64 57 714.6 0 57 65 59 714.6 0 59 66 53 714.6 0 53 67 54 714.6 0 54 68 45 714.6 0 45 69 52 714.6 0 52 70 55 714.6 0 55 71 60 714.6 0 60 72 55 714.6 0 55 73 47 714.6 0 47 74 34 714.6 0 34 75 38 714.6 0 38 76 68 714.6 0 68 77 50 714.6 0 50 78 44 714.6 0 44 79 53 714.6 0 53 80 42 714.6 0 42 81 33 714.6 0 33 82 48 714.6 0 48 83 61 714.6 0 61 84 74 714.6 0 74 85 159 714.6 0 159 86 115 714.6 0 115 87 32 714.6 0 32 88 25 714.6 0 25 89 31 714.6 0 31 90 43 714.6 0 43 91 41 714.6 0 41 92 39 714.6 0 39 93 24 714.6 0 24 94 44 714.6 0 44 95 35 714.6 0 35 96 34 714.6 0 34 97 39 714.6 0 39 98 28 714.6 0 28 99 32 714.6 0 32 100 32 714.6 0 32 101 31 714.6 0 31 102 54 714.6 0 54 103 30 714.6 0 30 104 24 714.6 0 24 105 34 714.6 0 34 106 44 714.6 0 44 107 36 714.6 0 36 108 28 714.6 0 28 109 39 714.6 0 39 110 35 714.6 0 35 111 34 714.6 0 34 112 138 714.6 0 138 113 149 714.6 0 149 114 195 714.6 0 195 115 45 714.6 0 45 116 35 714.6 0 35 117 34 714.6 0 34 118 57 714.6 0 57 119 56 714.6 0 56 120 26 714.6 0 26 121 46 714.6 0 46 122 25 714.6 0 25 123 39 714.6 0 39 124 45 714.6 0 45 125 50 714.6 0 50 126 52 714.6 0 52 127 53 714.6 0 53 128 71 714.6 0 71 129 53 714.6 0 53 130 63 714.6 0 63 131 38 714.6 0 38 132 59 714.6 0 59 133 105 714.6 0 105 134 112 714.6 0 112 135 57 714.6 0 57 136 91 714.6 0 91 137 45 714.6 0 45 138 41 714.6 0 41 139 49 714.6 0 49 140 63 714.6 0 63 141 104 714.6 0 104 142 939 714.6 0 939 143 107 714.6 0 107 144 37 714.6 0 37 145 52 714.6 0 52 146 37 714.6 0 37 147 75 714.6 0 75 148 52 714.6 0 52 149 56 714.6 0 56 150 41 714.6 0 41 151 39 714.6 0 39 152 45 714.6 0 45 153 69 714.6 0 69 154 85 714.6 0 85 155 43 714.6 0 43 156 30 714.6 0 30 157 30 714.6 0 30 158 40 714.6 0 40 159 36 714.6 0 36 160 42 714.6 0 42 161 38 714.6 0 38 162 38 714.6 0 38 163 54 714.6 0 54 164 38 714.6 0 38 165 26 714.6 0 26 166 37 714.6 0 37 167 40 714.6 0 40 168 31 714.6 0 31 169 48 714.6 0 48 170 38 714.6 0 38 171 32 714.6 0 32 172 43 714.6 0 43 173 40 714.6 0 40 174 40 714.6 0 40 175 37 714.6 0 37 176 35 714.6 0 35 177 33 714.6 0 33 178 34 714.6 0 34 179 38 714.6 0 38 180 28 714.6 0 28 181 38 714.6 0 38 182 42 714.6 0 42 183 45 714.6 0 45 184 54 714.6 0 54 185 28 714.6 0 28 186 25 714.6 0 25 187 38 714.6 0 38 188 35 714.6 0 35 189 36 714.6 0 36 190 38 714.6 0 38 191 42 714.6 0 42 192 41 714.6 0 41 193 43 714.6 0 43 194 28 714.6 0 28 195 33 714.6 0 33 196 55 714.6 0 55 197 34 714.6 0 34 198 36 714.6 0 36 199 30 714.6 0 30 200 26 714.6 0 26 201 42 714.6 0 42 202 44 714.6 0 44 203 23 714.6 0 23 204 34 714.6 0 34 205 42 714.6 0 42 206 39 714.6 0 39 207 34 714.6 0 34 208 54 714.6 0 54 209 29 714.6 0 29 210 32 714.6 0 32 211 29 714.6 0 29 212 48 714.6 0 48 213 34 714.6 0 34 214 57 714.6 0 57 215 48 714.6 0 48 216 53 714.6 0 53 217 35 714.6 0 35 218 30 714.6 0 30 219 37 714.6 0 37 220 47 714.6 0 47 221 34 714.6 0 34 222 30 714.6 0 30 223 31 714.6 0 31 224 210 714.6 0 210 225 32 714.6 0 32 226 34 714.6 0 34 227 35 714.6 0 35 228 33 714.6 0 33 229 25 714.6 0 25 230 37 714.6 0 37 231 30 714.6 0 30 232 43 714.6 0 43 233 33 714.6 0 33 234 33 714.6 0 33 235 57 714.6 0 57 236 45 714.6 0 45 237 47 714.6 0 47 238 40 714.6 0 40 239 44 714.6 0 44 240 33 714.6 0 33 241 28 714.6 0 28 242 31 714.6 0 31 243 28 714.6 0 28 244 32 714.6 0 32 245 46 714.6 0 46 246 37 714.6 0 37 247 34 714.6 0 34 248 25 714.6 0 25 249 28 714.6 0 28 250 29 714.6 0 29 251 27 714.6 0 27 252 47 714.6 0 47 253 40 714.6 0 40 254 30 714.6 0 30 255 49 714.6 0 49 256 79 714.6 0 79 257 107 714.6 0 107 258 44 714.6 0 44 259 24 714.6 0 24 260 32 714.6 0 32 261 74 714.6 0 74 262 22 714.6 0 22 263 27 714.6 0 27 264 34 714.6 0 34 265 18 714.6 0 18 266 35 714.6 0 35 267 25 714.6 0 25 268 23 714.6 0 23 269 27 714.6 0 27 270 24 714.6 0 24 271 27 714.6 0 27 272 19 714.6 0 19 273 32 714.6 0 32 274 86 714.6 0 86 275 38 714.6 0 38 276 33 714.6 0 33 277 29 714.6 0 29 278 42 714.6 0 42 279 34 714.6 0 34 280 19 714.6 0 19 281 30 714.6 0 30 282 33 714.6 0 33 283 19 714.6 0 19 284 34 714.6 0 34 285 64 714.6 0 64 286 44 714.6 0 44 287 55 714.6 0 55 288 34 714.6 0 34 289 27 714.6 0 27 290 27 714.6 0 27 291 109 714.6 0 109 292 50 714.6 0 50 293 41 714.6 0 41 294 29 714.6 0 29 295 28 714.6 0 28 296 26 714.6 0 26 297 26 714.6 0 26 298 83 714.6 0 83 299 194 714.6 0 194 300 346 714.6 0 346 301 31 714.6 0 31 Finished in 0.57 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 14,115 Reads with adapters: 1,866 (13.2%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 1,860 (13.2%) Total basepairs processed: 3,061,881 bp Total written (filtered): 508,923 bp (16.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1866 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 5 0.0 3 0 0 5 16 16 0.0 3 0 16 17 1746 0.0 3 1709 33 4 18 43 0.0 3 0 11 26 6 19 26 0.0 3 1 0 5 20 20 1 0.0 3 0 0 0 1 26 2 0.0 3 0 0 1 1 39 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 1 53 2 0.0 3 0 0 0 2 87 3 0.0 3 0 0 1 2 89 2 0.0 3 0 0 0 2 101 4 0.0 3 0 0 0 4 102 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 1,860 Reads with adapters: 139 (7.5%) Reads written (passing filters): 1,860 (100.0%) Total basepairs processed: 508,923 bp Total written (filtered): 508,505 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 139 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 49.6% C: 15.1% G: 33.1% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 138 29.1 0 138 4 1 7.3 0 1