This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.74 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 68,305 (2.3%) Reads that were too short: 9,245 (0.3%) Reads written (passing filters): 59,060 (2.0%) Total basepairs processed: 881,011,047 bp Total written (filtered): 14,843,527 bp (1.7%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 68305 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 377 714.6 0 377 7 738 714.6 0 738 8 23014 714.6 0 23014 9 8653 714.6 0 8653 10 131 714.6 0 131 11 53 714.6 0 53 12 57 714.6 0 57 13 58 714.6 0 58 14 62 714.6 0 62 15 73 714.6 0 73 16 85 714.6 0 85 17 54 714.6 0 54 18 77 714.6 0 77 19 58 714.6 0 58 20 56 714.6 0 56 21 54 714.6 0 54 22 67 714.6 0 67 23 58 714.6 0 58 24 50 714.6 0 50 25 54 714.6 0 54 26 53 714.6 0 53 27 47 714.6 0 47 28 47 714.6 0 47 29 56 714.6 0 56 30 53 714.6 0 53 31 62 714.6 0 62 32 48 714.6 0 48 33 55 714.6 0 55 34 45 714.6 0 45 35 53 714.6 0 53 36 53 714.6 0 53 37 56 714.6 0 56 38 51 714.6 0 51 39 63 714.6 0 63 40 91 714.6 0 91 41 45 714.6 0 45 42 90 714.6 0 90 43 49 714.6 0 49 44 45 714.6 0 45 45 55 714.6 0 55 46 70 714.6 0 70 47 48 714.6 0 48 48 84 714.6 0 84 49 4986 714.6 0 4986 50 727 714.6 0 727 51 42 714.6 0 42 52 54 714.6 0 54 53 49 714.6 0 49 54 49 714.6 0 49 55 38 714.6 0 38 56 22 714.6 0 22 57 35 714.6 0 35 58 27 714.6 0 27 59 30 714.6 0 30 60 38 714.6 0 38 61 55 714.6 0 55 62 30 714.6 0 30 63 32 714.6 0 32 64 36 714.6 0 36 65 36 714.6 0 36 66 37 714.6 0 37 67 43 714.6 0 43 68 44 714.6 0 44 69 14 714.6 0 14 70 44 714.6 0 44 71 32 714.6 0 32 72 24 714.6 0 24 73 33 714.6 0 33 74 28 714.6 0 28 75 28 714.6 0 28 76 23 714.6 0 23 77 26 714.6 0 26 78 33 714.6 0 33 79 33 714.6 0 33 80 44 714.6 0 44 81 34 714.6 0 34 82 91 714.6 0 91 83 95 714.6 0 95 84 80 714.6 0 80 85 94 714.6 0 94 86 103 714.6 0 103 87 60 714.6 0 60 88 43 714.6 0 43 89 89 714.6 0 89 90 70 714.6 0 70 91 187 714.6 0 187 92 175 714.6 0 175 93 100 714.6 0 100 94 78 714.6 0 78 95 91 714.6 0 91 96 55 714.6 0 55 97 101 714.6 0 101 98 293 714.6 0 293 99 86 714.6 0 86 100 44 714.6 0 44 101 34 714.6 0 34 102 52 714.6 0 52 103 45 714.6 0 45 104 72 714.6 0 72 105 85 714.6 0 85 106 88 714.6 0 88 107 97 714.6 0 97 108 46 714.6 0 46 109 75 714.6 0 75 110 180 714.6 0 180 111 3509 714.6 0 3509 112 211 714.6 0 211 113 238 714.6 0 238 114 88 714.6 0 88 115 270 714.6 0 270 116 372 714.6 0 372 117 302 714.6 0 302 118 1323 714.6 0 1323 119 751 714.6 0 751 120 58 714.6 0 58 121 48 714.6 0 48 122 58 714.6 0 58 123 38 714.6 0 38 124 46 714.6 0 46 125 107 714.6 0 107 126 98 714.6 0 98 127 108 714.6 0 108 128 57 714.6 0 57 129 51 714.6 0 51 130 57 714.6 0 57 131 84 714.6 0 84 132 74 714.6 0 74 133 81 714.6 0 81 134 54 714.6 0 54 135 61 714.6 0 61 136 49 714.6 0 49 137 46 714.6 0 46 138 72 714.6 0 72 139 74 714.6 0 74 140 50 714.6 0 50 141 54 714.6 0 54 142 72 714.6 0 72 143 64 714.6 0 64 144 78 714.6 0 78 145 82 714.6 0 82 146 95 714.6 0 95 147 76 714.6 0 76 148 118 714.6 0 118 149 92 714.6 0 92 150 82 714.6 0 82 151 229 714.6 0 229 152 595 714.6 0 595 153 282 714.6 0 282 154 107 714.6 0 107 155 101 714.6 0 101 156 67 714.6 0 67 157 97 714.6 0 97 158 51 714.6 0 51 159 84 714.6 0 84 160 45 714.6 0 45 161 73 714.6 0 73 162 114 714.6 0 114 163 98 714.6 0 98 164 93 714.6 0 93 165 62 714.6 0 62 166 55 714.6 0 55 167 73 714.6 0 73 168 77 714.6 0 77 169 85 714.6 0 85 170 79 714.6 0 79 171 102 714.6 0 102 172 117 714.6 0 117 173 82 714.6 0 82 174 59 714.6 0 59 175 55 714.6 0 55 176 63 714.6 0 63 177 83 714.6 0 83 178 96 714.6 0 96 179 133 714.6 0 133 180 211 714.6 0 211 181 189 714.6 0 189 182 153 714.6 0 153 183 91 714.6 0 91 184 90 714.6 0 90 185 67 714.6 0 67 186 43 714.6 0 43 187 55 714.6 0 55 188 73 714.6 0 73 189 66 714.6 0 66 190 83 714.6 0 83 191 86 714.6 0 86 192 90 714.6 0 90 193 53 714.6 0 53 194 44 714.6 0 44 195 89 714.6 0 89 196 67 714.6 0 67 197 65 714.6 0 65 198 46 714.6 0 46 199 84 714.6 0 84 200 107 714.6 0 107 201 40 714.6 0 40 202 52 714.6 0 52 203 40 714.6 0 40 204 59 714.6 0 59 205 69 714.6 0 69 206 71 714.6 0 71 207 59 714.6 0 59 208 62 714.6 0 62 209 58 714.6 0 58 210 73 714.6 0 73 211 91 714.6 0 91 212 105 714.6 0 105 213 118 714.6 0 118 214 116 714.6 0 116 215 115 714.6 0 115 216 108 714.6 0 108 217 140 714.6 0 140 218 294 714.6 0 294 219 374 714.6 0 374 220 127 714.6 0 127 221 110 714.6 0 110 222 68 714.6 0 68 223 75 714.6 0 75 224 105 714.6 0 105 225 115 714.6 0 115 226 112 714.6 0 112 227 86 714.6 0 86 228 100 714.6 0 100 229 152 714.6 0 152 230 97 714.6 0 97 231 119 714.6 0 119 232 155 714.6 0 155 233 236 714.6 0 236 234 125 714.6 0 125 235 204 714.6 0 204 236 162 714.6 0 162 237 178 714.6 0 178 238 103 714.6 0 103 239 92 714.6 0 92 240 63 714.6 0 63 241 70 714.6 0 70 242 54 714.6 0 54 243 61 714.6 0 61 244 84 714.6 0 84 245 151 714.6 0 151 246 145 714.6 0 145 247 106 714.6 0 106 248 111 714.6 0 111 249 129 714.6 0 129 250 104 714.6 0 104 251 53 714.6 0 53 252 74 714.6 0 74 253 75 714.6 0 75 254 172 714.6 0 172 255 169 714.6 0 169 256 145 714.6 0 145 257 75 714.6 0 75 258 64 714.6 0 64 259 67 714.6 0 67 260 44 714.6 0 44 261 39 714.6 0 39 262 37 714.6 0 37 263 42 714.6 0 42 264 63 714.6 0 63 265 95 714.6 0 95 266 82 714.6 0 82 267 79 714.6 0 79 268 75 714.6 0 75 269 75 714.6 0 75 270 73 714.6 0 73 271 47 714.6 0 47 272 57 714.6 0 57 273 39 714.6 0 39 274 43 714.6 0 43 275 63 714.6 0 63 276 74 714.6 0 74 277 62 714.6 0 62 278 51 714.6 0 51 279 72 714.6 0 72 280 90 714.6 0 90 281 108 714.6 0 108 282 88 714.6 0 88 283 99 714.6 0 99 284 114 714.6 0 114 285 61 714.6 0 61 286 67 714.6 0 67 287 100 714.6 0 100 288 85 714.6 0 85 289 79 714.6 0 79 290 52 714.6 0 52 291 52 714.6 0 52 292 64 714.6 0 64 293 95 714.6 0 95 294 75 714.6 0 75 295 66 714.6 0 66 296 60 714.6 0 60 297 42 714.6 0 42 298 39 714.6 0 39 299 29 714.6 0 29 300 52 714.6 0 52 301 19 714.6 0 19 Finished in 2.87 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 59,060 Reads with adapters: 9,050 (15.3%) Reads that were too short: 34 (0.1%) Reads written (passing filters): 9,016 (15.3%) Total basepairs processed: 14,843,527 bp Total written (filtered): 2,452,332 bp (16.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9050 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 107 0.0 3 0 90 11 2 4 20 8868 0.0 4 8312 474 57 13 12 21 21 0.0 4 4 12 1 1 3 22 1 0.0 4 0 0 1 23 7 0.0 4 0 0 0 5 2 24 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 1 70 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 2 127 1 0.0 4 0 0 0 0 1 132 2 0.0 4 0 0 0 0 2 133 2 0.0 4 0 0 0 0 2 134 2 0.0 4 0 0 0 0 2 137 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 185 5 0.0 4 0 0 0 0 5 186 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 2 0.0 4 0 0 0 0 2 241 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.30 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 9,016 Reads with adapters: 487 (5.4%) Reads written (passing filters): 9,016 (100.0%) Total basepairs processed: 2,452,332 bp Total written (filtered): 2,446,376 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 487 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 4.3% G: 89.5% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 96 0.0 0 9 87 10 245 0.0 1 13 232 11 141 0.0 1 1 140 150 1 0.0 1 0 1 223 2 0.0 1 2 225 1 0.0 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.