This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.45 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 262,702 (9.0%) Reads that were too short: 8,358 (0.3%) Reads written (passing filters): 254,344 (8.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 62,194,673 bp (7.1%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 262702 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 380 714.6 0 380 7 5679 714.6 0 5679 8 18808 714.6 0 18808 9 231 714.6 0 231 10 233 714.6 0 233 11 142 714.6 0 142 12 141 714.6 0 141 13 215 714.6 0 215 14 211 714.6 0 211 15 217 714.6 0 217 16 236 714.6 0 236 17 296 714.6 0 296 18 317 714.6 0 317 19 417 714.6 0 417 20 5859 714.6 0 5859 21 15149 714.6 0 15149 22 11100 714.6 0 11100 23 155 714.6 0 155 24 132 714.6 0 132 25 160 714.6 0 160 26 88 714.6 0 88 27 81 714.6 0 81 28 69 714.6 0 69 29 136 714.6 0 136 30 117 714.6 0 117 31 177 714.6 0 177 32 196 714.6 0 196 33 292 714.6 0 292 34 331 714.6 0 331 35 653 714.6 0 653 36 1669 714.6 0 1669 37 345 714.6 0 345 38 547 714.6 0 547 39 1350 714.6 0 1350 40 586 714.6 0 586 41 564 714.6 0 564 42 885 714.6 0 885 43 842 714.6 0 842 44 1033 714.6 0 1033 45 915 714.6 0 915 46 1144 714.6 0 1144 47 1406 714.6 0 1406 48 2551 714.6 0 2551 49 27123 714.6 0 27123 50 76167 714.6 0 76167 51 43964 714.6 0 43964 52 286 714.6 0 286 53 31 714.6 0 31 54 56 714.6 0 56 55 52 714.6 0 52 56 57 714.6 0 57 57 41 714.6 0 41 58 50 714.6 0 50 59 49 714.6 0 49 60 45 714.6 0 45 61 23 714.6 0 23 62 58 714.6 0 58 63 97 714.6 0 97 64 134 714.6 0 134 65 126 714.6 0 126 66 32 714.6 0 32 67 23 714.6 0 23 68 56 714.6 0 56 69 26 714.6 0 26 70 33 714.6 0 33 71 44 714.6 0 44 72 35 714.6 0 35 73 35 714.6 0 35 74 35 714.6 0 35 75 32 714.6 0 32 76 31 714.6 0 31 77 45 714.6 0 45 78 33 714.6 0 33 79 37 714.6 0 37 80 48 714.6 0 48 81 45 714.6 0 45 82 56 714.6 0 56 83 43 714.6 0 43 84 102 714.6 0 102 85 104 714.6 0 104 86 62 714.6 0 62 87 60 714.6 0 60 88 75 714.6 0 75 89 40 714.6 0 40 90 64 714.6 0 64 91 35 714.6 0 35 92 37 714.6 0 37 93 48 714.6 0 48 94 66 714.6 0 66 95 37 714.6 0 37 96 43 714.6 0 43 97 32 714.6 0 32 98 34 714.6 0 34 99 25 714.6 0 25 100 32 714.6 0 32 101 28 714.6 0 28 102 46 714.6 0 46 103 37 714.6 0 37 104 21 714.6 0 21 105 19 714.6 0 19 106 30 714.6 0 30 107 30 714.6 0 30 108 12 714.6 0 12 109 21 714.6 0 21 110 30 714.6 0 30 111 36 714.6 0 36 112 45 714.6 0 45 113 37 714.6 0 37 114 22 714.6 0 22 115 28 714.6 0 28 116 32 714.6 0 32 117 161 714.6 0 161 118 128 714.6 0 128 119 118 714.6 0 118 120 97 714.6 0 97 121 33 714.6 0 33 122 40 714.6 0 40 123 47 714.6 0 47 124 64 714.6 0 64 125 36 714.6 0 36 126 24 714.6 0 24 127 45 714.6 0 45 128 168 714.6 0 168 129 36 714.6 0 36 130 53 714.6 0 53 131 52 714.6 0 52 132 42 714.6 0 42 133 42 714.6 0 42 134 49 714.6 0 49 135 50 714.6 0 50 136 41 714.6 0 41 137 46 714.6 0 46 138 50 714.6 0 50 139 42 714.6 0 42 140 39 714.6 0 39 141 31 714.6 0 31 142 33 714.6 0 33 143 48 714.6 0 48 144 62 714.6 0 62 145 47 714.6 0 47 146 54 714.6 0 54 147 94 714.6 0 94 148 24 714.6 0 24 149 33 714.6 0 33 150 30 714.6 0 30 151 28 714.6 0 28 152 30 714.6 0 30 153 47 714.6 0 47 154 27 714.6 0 27 155 39 714.6 0 39 156 39 714.6 0 39 157 56 714.6 0 56 158 71 714.6 0 71 159 50 714.6 0 50 160 40 714.6 0 40 161 50 714.6 0 50 162 40 714.6 0 40 163 37 714.6 0 37 164 31 714.6 0 31 165 53 714.6 0 53 166 40 714.6 0 40 167 52 714.6 0 52 168 54 714.6 0 54 169 35 714.6 0 35 170 41 714.6 0 41 171 69 714.6 0 69 172 56 714.6 0 56 173 66 714.6 0 66 174 40 714.6 0 40 175 59 714.6 0 59 176 93 714.6 0 93 177 98 714.6 0 98 178 115 714.6 0 115 179 167 714.6 0 167 180 222 714.6 0 222 181 318 714.6 0 318 182 4650 714.6 0 4650 183 3736 714.6 0 3736 184 635 714.6 0 635 185 104 714.6 0 104 186 98 714.6 0 98 187 85 714.6 0 85 188 104 714.6 0 104 189 94 714.6 0 94 190 141 714.6 0 141 191 146 714.6 0 146 192 174 714.6 0 174 193 481 714.6 0 481 194 3190 714.6 0 3190 195 1495 714.6 0 1495 196 3665 714.6 0 3665 197 4081 714.6 0 4081 198 755 714.6 0 755 199 42 714.6 0 42 200 44 714.6 0 44 201 29 714.6 0 29 202 29 714.6 0 29 203 20 714.6 0 20 204 35 714.6 0 35 205 73 714.6 0 73 206 54 714.6 0 54 207 50 714.6 0 50 208 47 714.6 0 47 209 31 714.6 0 31 210 23 714.6 0 23 211 35 714.6 0 35 212 49 714.6 0 49 213 78 714.6 0 78 214 145 714.6 0 145 215 220 714.6 0 220 216 145 714.6 0 145 217 115 714.6 0 115 218 58 714.6 0 58 219 116 714.6 0 116 220 134 714.6 0 134 221 123 714.6 0 123 222 88 714.6 0 88 223 66 714.6 0 66 224 35 714.6 0 35 225 45 714.6 0 45 226 51 714.6 0 51 227 63 714.6 0 63 228 66 714.6 0 66 229 46 714.6 0 46 230 46 714.6 0 46 231 52 714.6 0 52 232 36 714.6 0 36 233 44 714.6 0 44 234 61 714.6 0 61 235 55 714.6 0 55 236 80 714.6 0 80 237 76 714.6 0 76 238 76 714.6 0 76 239 88 714.6 0 88 240 125 714.6 0 125 241 147 714.6 0 147 242 48 714.6 0 48 243 25 714.6 0 25 244 34 714.6 0 34 245 39 714.6 0 39 246 99 714.6 0 99 247 69 714.6 0 69 248 55 714.6 0 55 249 31 714.6 0 31 250 53 714.6 0 53 251 162 714.6 0 162 252 119 714.6 0 119 253 64 714.6 0 64 254 45 714.6 0 45 255 51 714.6 0 51 256 81 714.6 0 81 257 88 714.6 0 88 258 72 714.6 0 72 259 53 714.6 0 53 260 44 714.6 0 44 261 119 714.6 0 119 262 23 714.6 0 23 263 161 714.6 0 161 264 278 714.6 0 278 265 441 714.6 0 441 266 486 714.6 0 486 267 131 714.6 0 131 268 91 714.6 0 91 269 53 714.6 0 53 270 61 714.6 0 61 271 92 714.6 0 92 272 22 714.6 0 22 273 42 714.6 0 42 274 42 714.6 0 42 275 48 714.6 0 48 276 33 714.6 0 33 277 27 714.6 0 27 278 109 714.6 0 109 279 56 714.6 0 56 280 46 714.6 0 46 281 42 714.6 0 42 282 56 714.6 0 56 283 49 714.6 0 49 284 75 714.6 0 75 285 75 714.6 0 75 286 62 714.6 0 62 287 42 714.6 0 42 288 160 714.6 0 160 289 308 714.6 0 308 290 397 714.6 0 397 291 55 714.6 0 55 292 47 714.6 0 47 293 122 714.6 0 122 294 111 714.6 0 111 295 57 714.6 0 57 296 21 714.6 0 21 297 18 714.6 0 18 298 20 714.6 0 20 299 38 714.6 0 38 300 56 714.6 0 56 301 28 714.6 0 28 Finished in 11.88 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 254,344 Reads with adapters: 20,281 (8.0%) Reads that were too short: 169 (0.1%) Reads written (passing filters): 20,112 (7.9%) Total basepairs processed: 62,194,673 bp Total written (filtered): 5,433,399 bp (8.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 20281 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 7 0.0 3 0 0 7 19 249 0.0 3 0 230 10 5 4 20 19238 0.0 4 18646 492 64 23 13 21 46 0.0 4 8 33 2 0 3 22 7 0.0 4 0 1 3 3 23 19 0.0 4 0 0 0 15 4 38 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 55 2 0.0 4 0 0 0 1 1 57 1 0.0 4 1 65 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 449 0.0 4 0 0 0 0 449 98 4 0.0 4 0 0 0 0 4 101 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 1 114 75 0.0 4 0 0 0 0 75 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 140 3 0.0 4 0 0 0 0 3 141 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 160 2 0.0 4 0 0 0 0 2 161 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 175 2 0.0 4 0 0 0 0 2 177 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 185 4 0.0 4 0 0 0 0 4 186 63 0.0 4 0 0 0 3 60 187 6 0.0 4 0 0 0 0 6 188 4 0.0 4 0 0 0 0 4 189 2 0.0 4 0 0 0 0 2 190 2 0.0 4 0 0 0 0 2 191 2 0.0 4 0 0 0 0 2 192 2 0.0 4 0 0 0 1 1 195 1 0.0 4 0 0 0 0 1 196 3 0.0 4 0 0 0 0 3 197 2 0.0 4 0 0 0 1 1 206 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 209 1 0.0 4 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 5 0.0 4 0 0 0 0 5 216 2 0.0 4 0 0 0 0 2 220 3 0.0 4 0 0 0 0 3 221 2 0.0 4 0 0 0 0 2 222 3 0.0 4 0 0 0 0 3 223 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 1 8 229 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 240 5 0.0 4 0 0 0 0 5 242 2 0.0 4 0 0 0 0 2 244 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 247 4 0.0 4 0 0 0 0 4 250 2 0.0 4 0 0 0 0 2 251 3 0.0 4 0 0 0 0 3 252 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 269 2 0.0 4 0 0 0 0 2 273 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.69 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 20,112 Reads with adapters: 1,872 (9.3%) Reads written (passing filters): 20,112 (100.0%) Total basepairs processed: 5,433,399 bp Total written (filtered): 5,413,240 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1872 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 3.3% G: 91.2% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 22 314.2 0 22 4 5 78.6 0 5 6 77 4.9 0 77 9 4 0.1 0 1 3 10 83 0.0 1 2 81 11 1677 0.0 1 7 1670 12 2 0.0 1 0 2 14 1 0.0 1 0 1 260 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.