This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.36 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 305,217 (10.4%) Reads that were too short: 131,451 (4.5%) Reads written (passing filters): 173,766 (5.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 33,233,468 bp (3.8%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 305217 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 10943 714.6 0 10943 7 547 714.6 0 547 8 26131 714.6 0 26131 9 58 714.6 0 58 10 33 714.6 0 33 11 21 714.6 0 21 12 26 714.6 0 26 13 28 714.6 0 28 14 23 714.6 0 23 15 28 714.6 0 28 16 35 714.6 0 35 17 24 714.6 0 24 18 27 714.6 0 27 19 38 714.6 0 38 20 25 714.6 0 25 21 24 714.6 0 24 22 19 714.6 0 19 23 27 714.6 0 27 24 20 714.6 0 20 25 25 714.6 0 25 26 23 714.6 0 23 27 20 714.6 0 20 28 24 714.6 0 24 29 30 714.6 0 30 30 31 714.6 0 31 31 24 714.6 0 24 32 29 714.6 0 29 33 78 714.6 0 78 34 32 714.6 0 32 35 49 714.6 0 49 36 28 714.6 0 28 37 22 714.6 0 22 38 24 714.6 0 24 39 111 714.6 0 111 40 47 714.6 0 47 41 33 714.6 0 33 42 46 714.6 0 46 43 44 714.6 0 44 44 26 714.6 0 26 45 32 714.6 0 32 46 27 714.6 0 27 47 29 714.6 0 29 48 28 714.6 0 28 49 20 714.6 0 20 50 20 714.6 0 20 51 40 714.6 0 40 52 55 714.6 0 55 53 29 714.6 0 29 54 48 714.6 0 48 55 26 714.6 0 26 56 27 714.6 0 27 57 53 714.6 0 53 58 40 714.6 0 40 59 35 714.6 0 35 60 29 714.6 0 29 61 36 714.6 0 36 62 54 714.6 0 54 63 82 714.6 0 82 64 81 714.6 0 81 65 130 714.6 0 130 66 160 714.6 0 160 67 231 714.6 0 231 68 662 714.6 0 662 69 9788 714.6 0 9788 70 6964 714.6 0 6964 71 1184 714.6 0 1184 72 31 714.6 0 31 73 14 714.6 0 14 74 20 714.6 0 20 75 28 714.6 0 28 76 21 714.6 0 21 77 34 714.6 0 34 78 36 714.6 0 36 79 54 714.6 0 54 80 296 714.6 0 296 81 352 714.6 0 352 82 416 714.6 0 416 83 231 714.6 0 231 84 460 714.6 0 460 85 642 714.6 0 642 86 340 714.6 0 340 87 335 714.6 0 335 88 356 714.6 0 356 89 140 714.6 0 140 90 106 714.6 0 106 91 99 714.6 0 99 92 101 714.6 0 101 93 182 714.6 0 182 94 306 714.6 0 306 95 296 714.6 0 296 96 672 714.6 0 672 97 685 714.6 0 685 98 557 714.6 0 557 99 340 714.6 0 340 100 731 714.6 0 731 101 1147 714.6 0 1147 102 3370 714.6 0 3370 103 2002 714.6 0 2002 104 891 714.6 0 891 105 366 714.6 0 366 106 340 714.6 0 340 107 204 714.6 0 204 108 530 714.6 0 530 109 284 714.6 0 284 110 200 714.6 0 200 111 394 714.6 0 394 112 599 714.6 0 599 113 475 714.6 0 475 114 615 714.6 0 615 115 465 714.6 0 465 116 384 714.6 0 384 117 671 714.6 0 671 118 972 714.6 0 972 119 830 714.6 0 830 120 537 714.6 0 537 121 580 714.6 0 580 122 705 714.6 0 705 123 942 714.6 0 942 124 929 714.6 0 929 125 674 714.6 0 674 126 710 714.6 0 710 127 710 714.6 0 710 128 580 714.6 0 580 129 590 714.6 0 590 130 791 714.6 0 791 131 933 714.6 0 933 132 1789 714.6 0 1789 133 1717 714.6 0 1717 134 1306 714.6 0 1306 135 748 714.6 0 748 136 1113 714.6 0 1113 137 1490 714.6 0 1490 138 1813 714.6 0 1813 139 1553 714.6 0 1553 140 962 714.6 0 962 141 752 714.6 0 752 142 837 714.6 0 837 143 982 714.6 0 982 144 995 714.6 0 995 145 765 714.6 0 765 146 770 714.6 0 770 147 884 714.6 0 884 148 1399 714.6 0 1399 149 1002 714.6 0 1002 150 1040 714.6 0 1040 151 1089 714.6 0 1089 152 845 714.6 0 845 153 869 714.6 0 869 154 877 714.6 0 877 155 669 714.6 0 669 156 1186 714.6 0 1186 157 1508 714.6 0 1508 158 1710 714.6 0 1710 159 1377 714.6 0 1377 160 1183 714.6 0 1183 161 1087 714.6 0 1087 162 1195 714.6 0 1195 163 982 714.6 0 982 164 952 714.6 0 952 165 665 714.6 0 665 166 766 714.6 0 766 167 991 714.6 0 991 168 1392 714.6 0 1392 169 1480 714.6 0 1480 170 1201 714.6 0 1201 171 1168 714.6 0 1168 172 1023 714.6 0 1023 173 900 714.6 0 900 174 792 714.6 0 792 175 946 714.6 0 946 176 1944 714.6 0 1944 177 2688 714.6 0 2688 178 2329 714.6 0 2329 179 1487 714.6 0 1487 180 1099 714.6 0 1099 181 907 714.6 0 907 182 1204 714.6 0 1204 183 1183 714.6 0 1183 184 1232 714.6 0 1232 185 1179 714.6 0 1179 186 1223 714.6 0 1223 187 1526 714.6 0 1526 188 1819 714.6 0 1819 189 2099 714.6 0 2099 190 1052 714.6 0 1052 191 757 714.6 0 757 192 759 714.6 0 759 193 904 714.6 0 904 194 937 714.6 0 937 195 1296 714.6 0 1296 196 1724 714.6 0 1724 197 1066 714.6 0 1066 198 913 714.6 0 913 199 979 714.6 0 979 200 1340 714.6 0 1340 201 1316 714.6 0 1316 202 947 714.6 0 947 203 701 714.6 0 701 204 945 714.6 0 945 205 1201 714.6 0 1201 206 1046 714.6 0 1046 207 1004 714.6 0 1004 208 1051 714.6 0 1051 209 1208 714.6 0 1208 210 1264 714.6 0 1264 211 1138 714.6 0 1138 212 1253 714.6 0 1253 213 1442 714.6 0 1442 214 1443 714.6 0 1443 215 1359 714.6 0 1359 216 1184 714.6 0 1184 217 1348 714.6 0 1348 218 1277 714.6 0 1277 219 1297 714.6 0 1297 220 1176 714.6 0 1176 221 1322 714.6 0 1322 222 1629 714.6 0 1629 223 1447 714.6 0 1447 224 1568 714.6 0 1568 225 1421 714.6 0 1421 226 1331 714.6 0 1331 227 1097 714.6 0 1097 228 1078 714.6 0 1078 229 1224 714.6 0 1224 230 1217 714.6 0 1217 231 1007 714.6 0 1007 232 979 714.6 0 979 233 1147 714.6 0 1147 234 1077 714.6 0 1077 235 915 714.6 0 915 236 921 714.6 0 921 237 1387 714.6 0 1387 238 1441 714.6 0 1441 239 1318 714.6 0 1318 240 1443 714.6 0 1443 241 1564 714.6 0 1564 242 1580 714.6 0 1580 243 1246 714.6 0 1246 244 1754 714.6 0 1754 245 1520 714.6 0 1520 246 1496 714.6 0 1496 247 1110 714.6 0 1110 248 1409 714.6 0 1409 249 1636 714.6 0 1636 250 1516 714.6 0 1516 251 916 714.6 0 916 252 456 714.6 0 456 253 680 714.6 0 680 254 1259 714.6 0 1259 255 1357 714.6 0 1357 256 1597 714.6 0 1597 257 1565 714.6 0 1565 258 1467 714.6 0 1467 259 1289 714.6 0 1289 260 1288 714.6 0 1288 261 1128 714.6 0 1128 262 921 714.6 0 921 263 1165 714.6 0 1165 264 1439 714.6 0 1439 265 1621 714.6 0 1621 266 1934 714.6 0 1934 267 1317 714.6 0 1317 268 1234 714.6 0 1234 269 2255 714.6 0 2255 270 1976 714.6 0 1976 271 1257 714.6 0 1257 272 771 714.6 0 771 273 1133 714.6 0 1133 274 1566 714.6 0 1566 275 1418 714.6 0 1418 276 1849 714.6 0 1849 277 1833 714.6 0 1833 278 1351 714.6 0 1351 279 1092 714.6 0 1092 280 889 714.6 0 889 281 1689 714.6 0 1689 282 1885 714.6 0 1885 283 1079 714.6 0 1079 284 1098 714.6 0 1098 285 1444 714.6 0 1444 286 1414 714.6 0 1414 287 1396 714.6 0 1396 288 1841 714.6 0 1841 289 1806 714.6 0 1806 290 1076 714.6 0 1076 291 762 714.6 0 762 292 1064 714.6 0 1064 293 1269 714.6 0 1269 294 1847 714.6 0 1847 295 2053 714.6 0 2053 296 1335 714.6 0 1335 297 980 714.6 0 980 298 989 714.6 0 989 299 1071 714.6 0 1071 300 1088 714.6 0 1088 301 2158 714.6 0 2158 Finished in 6.07 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 173,766 Reads with adapters: 26,739 (15.4%) Reads that were too short: 35 (0.0%) Reads written (passing filters): 26,704 (15.4%) Total basepairs processed: 33,233,468 bp Total written (filtered): 7,358,470 bp (22.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26739 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 10 0.0 3 0 0 8 2 16 157 0.0 3 0 149 6 2 17 18235 0.0 3 17928 301 5 1 18 8215 0.0 3 8003 207 4 1 19 29 0.0 3 3 15 10 1 20 17 0.0 3 0 0 1 16 21 7 0.0 3 0 0 0 7 22 1 0.0 3 0 0 0 1 25 1 0.0 3 1 26 1 0.0 3 1 34 2 0.0 3 0 0 0 2 35 1 0.0 3 1 37 3 0.0 3 0 0 0 3 41 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 282 3 0.0 3 0 0 0 3 283 2 0.0 3 0 0 0 2 287 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.93 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 26,704 Reads with adapters: 1,004 (3.8%) Reads written (passing filters): 26,704 (100.0%) Total basepairs processed: 7,358,470 bp Total written (filtered): 7,355,445 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1004 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.6% C: 12.9% G: 51.6% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 992 417.2 0 992 4 11 104.3 0 11 5 1 26.1 0 1