This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.92 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 253,686 (8.7%) Reads that were too short: 110,869 (3.8%) Reads written (passing filters): 142,817 (4.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 24,201,065 bp (2.7%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 253686 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 96 714.6 0 96 7 5373 714.6 0 5373 8 41 714.6 0 41 9 15 714.6 0 15 10 19 714.6 0 19 11 19 714.6 0 19 12 17 714.6 0 17 13 19 714.6 0 19 14 17 714.6 0 17 15 10 714.6 0 10 16 22 714.6 0 22 17 13 714.6 0 13 18 9 714.6 0 9 19 13 714.6 0 13 20 24 714.6 0 24 21 20 714.6 0 20 22 18 714.6 0 18 23 13 714.6 0 13 24 19 714.6 0 19 25 23 714.6 0 23 26 21 714.6 0 21 27 35 714.6 0 35 28 32 714.6 0 32 29 220 714.6 0 220 30 214 714.6 0 214 31 50 714.6 0 50 32 9 714.6 0 9 33 18 714.6 0 18 34 18 714.6 0 18 35 17 714.6 0 17 36 28 714.6 0 28 37 25 714.6 0 25 38 19 714.6 0 19 39 27 714.6 0 27 40 31 714.6 0 31 41 16 714.6 0 16 42 22 714.6 0 22 43 20 714.6 0 20 44 25 714.6 0 25 45 16 714.6 0 16 46 25 714.6 0 25 47 24 714.6 0 24 48 20 714.6 0 20 49 30 714.6 0 30 50 24 714.6 0 24 51 26 714.6 0 26 52 25 714.6 0 25 53 31 714.6 0 31 54 21 714.6 0 21 55 17 714.6 0 17 56 22 714.6 0 22 57 13 714.6 0 13 58 18 714.6 0 18 59 15 714.6 0 15 60 21 714.6 0 21 61 27 714.6 0 27 62 18 714.6 0 18 63 23 714.6 0 23 64 18 714.6 0 18 65 28 714.6 0 28 66 27 714.6 0 27 67 43 714.6 0 43 68 38 714.6 0 38 69 59 714.6 0 59 70 557 714.6 0 557 71 1207 714.6 0 1207 72 79 714.6 0 79 73 19 714.6 0 19 74 23 714.6 0 23 75 12 714.6 0 12 76 16 714.6 0 16 77 15 714.6 0 15 78 62 714.6 0 62 79 306 714.6 0 306 80 222 714.6 0 222 81 1434 714.6 0 1434 82 866 714.6 0 866 83 960 714.6 0 960 84 209 714.6 0 209 85 398 714.6 0 398 86 112 714.6 0 112 87 98 714.6 0 98 88 66 714.6 0 66 89 96 714.6 0 96 90 112 714.6 0 112 91 153 714.6 0 153 92 480 714.6 0 480 93 642 714.6 0 642 94 737 714.6 0 737 95 622 714.6 0 622 96 524 714.6 0 524 97 259 714.6 0 259 98 862 714.6 0 862 99 3016 714.6 0 3016 100 669 714.6 0 669 101 317 714.6 0 317 102 485 714.6 0 485 103 268 714.6 0 268 104 161 714.6 0 161 105 88 714.6 0 88 106 182 714.6 0 182 107 1556 714.6 0 1556 108 834 714.6 0 834 109 426 714.6 0 426 110 558 714.6 0 558 111 860 714.6 0 860 112 480 714.6 0 480 113 654 714.6 0 654 114 1103 714.6 0 1103 115 1386 714.6 0 1386 116 1231 714.6 0 1231 117 999 714.6 0 999 118 715 714.6 0 715 119 792 714.6 0 792 120 718 714.6 0 718 121 623 714.6 0 623 122 611 714.6 0 611 123 953 714.6 0 953 124 9359 714.6 0 9359 125 13653 714.6 0 13653 126 16983 714.6 0 16983 127 794 714.6 0 794 128 567 714.6 0 567 129 1385 714.6 0 1385 130 1177 714.6 0 1177 131 851 714.6 0 851 132 515 714.6 0 515 133 640 714.6 0 640 134 649 714.6 0 649 135 811 714.6 0 811 136 943 714.6 0 943 137 670 714.6 0 670 138 642 714.6 0 642 139 853 714.6 0 853 140 965 714.6 0 965 141 662 714.6 0 662 142 594 714.6 0 594 143 623 714.6 0 623 144 745 714.6 0 745 145 598 714.6 0 598 146 641 714.6 0 641 147 547 714.6 0 547 148 764 714.6 0 764 149 2070 714.6 0 2070 150 3452 714.6 0 3452 151 3651 714.6 0 3651 152 628 714.6 0 628 153 674 714.6 0 674 154 704 714.6 0 704 155 654 714.6 0 654 156 1123 714.6 0 1123 157 979 714.6 0 979 158 729 714.6 0 729 159 599 714.6 0 599 160 616 714.6 0 616 161 612 714.6 0 612 162 779 714.6 0 779 163 711 714.6 0 711 164 920 714.6 0 920 165 831 714.6 0 831 166 838 714.6 0 838 167 676 714.6 0 676 168 698 714.6 0 698 169 1055 714.6 0 1055 170 764 714.6 0 764 171 622 714.6 0 622 172 523 714.6 0 523 173 557 714.6 0 557 174 493 714.6 0 493 175 544 714.6 0 544 176 656 714.6 0 656 177 695 714.6 0 695 178 812 714.6 0 812 179 745 714.6 0 745 180 682 714.6 0 682 181 1595 714.6 0 1595 182 1268 714.6 0 1268 183 990 714.6 0 990 184 732 714.6 0 732 185 934 714.6 0 934 186 893 714.6 0 893 187 545 714.6 0 545 188 684 714.6 0 684 189 821 714.6 0 821 190 918 714.6 0 918 191 770 714.6 0 770 192 897 714.6 0 897 193 841 714.6 0 841 194 521 714.6 0 521 195 618 714.6 0 618 196 1264 714.6 0 1264 197 1213 714.6 0 1213 198 836 714.6 0 836 199 466 714.6 0 466 200 375 714.6 0 375 201 804 714.6 0 804 202 1037 714.6 0 1037 203 808 714.6 0 808 204 933 714.6 0 933 205 1029 714.6 0 1029 206 1078 714.6 0 1078 207 901 714.6 0 901 208 983 714.6 0 983 209 1063 714.6 0 1063 210 1078 714.6 0 1078 211 923 714.6 0 923 212 883 714.6 0 883 213 826 714.6 0 826 214 967 714.6 0 967 215 953 714.6 0 953 216 1033 714.6 0 1033 217 993 714.6 0 993 218 942 714.6 0 942 219 799 714.6 0 799 220 733 714.6 0 733 221 799 714.6 0 799 222 1187 714.6 0 1187 223 1499 714.6 0 1499 224 1895 714.6 0 1895 225 6403 714.6 0 6403 226 11189 714.6 0 11189 227 2780 714.6 0 2780 228 884 714.6 0 884 229 639 714.6 0 639 230 798 714.6 0 798 231 853 714.6 0 853 232 827 714.6 0 827 233 1000 714.6 0 1000 234 1480 714.6 0 1480 235 1405 714.6 0 1405 236 1169 714.6 0 1169 237 1126 714.6 0 1126 238 1170 714.6 0 1170 239 889 714.6 0 889 240 701 714.6 0 701 241 868 714.6 0 868 242 977 714.6 0 977 243 798 714.6 0 798 244 905 714.6 0 905 245 1159 714.6 0 1159 246 1034 714.6 0 1034 247 807 714.6 0 807 248 420 714.6 0 420 249 370 714.6 0 370 250 668 714.6 0 668 251 1058 714.6 0 1058 252 802 714.6 0 802 253 531 714.6 0 531 254 916 714.6 0 916 255 841 714.6 0 841 256 896 714.6 0 896 257 962 714.6 0 962 258 844 714.6 0 844 259 707 714.6 0 707 260 1054 714.6 0 1054 261 1242 714.6 0 1242 262 1005 714.6 0 1005 263 802 714.6 0 802 264 738 714.6 0 738 265 665 714.6 0 665 266 1027 714.6 0 1027 267 972 714.6 0 972 268 527 714.6 0 527 269 513 714.6 0 513 270 1235 714.6 0 1235 271 1263 714.6 0 1263 272 859 714.6 0 859 273 755 714.6 0 755 274 940 714.6 0 940 275 667 714.6 0 667 276 650 714.6 0 650 277 825 714.6 0 825 278 965 714.6 0 965 279 952 714.6 0 952 280 559 714.6 0 559 281 647 714.6 0 647 282 1140 714.6 0 1140 283 947 714.6 0 947 284 768 714.6 0 768 285 1113 714.6 0 1113 286 949 714.6 0 949 287 608 714.6 0 608 288 586 714.6 0 586 289 766 714.6 0 766 290 1105 714.6 0 1105 291 1080 714.6 0 1080 292 804 714.6 0 804 293 831 714.6 0 831 294 848 714.6 0 848 295 929 714.6 0 929 296 1320 714.6 0 1320 297 1479 714.6 0 1479 298 1475 714.6 0 1475 299 1190 714.6 0 1190 300 811 714.6 0 811 301 1040 714.6 0 1040 Finished in 4.62 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 142,817 Reads with adapters: 5,915 (4.1%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 5,883 (4.1%) Total basepairs processed: 24,201,065 bp Total written (filtered): 1,555,348 bp (6.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5915 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 42 0.0 3 0 41 1 17 5367 0.0 3 5310 54 3 18 17 0.0 3 3 12 2 19 5 0.0 3 0 0 3 2 20 2 0.0 3 0 0 0 2 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 32 0.0 3 0 0 1 31 44 1 0.0 3 0 0 0 1 45 9 0.0 3 0 0 0 9 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 370 0.0 3 0 0 0 370 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 102 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 1 110 6 0.0 3 0 0 0 6 111 3 0.0 3 0 0 0 3 112 1 0.0 3 0 0 0 1 113 7 0.0 3 0 0 0 7 116 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 144 6 0.0 3 0 0 0 6 154 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 5,883 Reads with adapters: 395 (6.7%) Reads written (passing filters): 5,883 (100.0%) Total basepairs processed: 1,555,348 bp Total written (filtered): 1,554,159 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 395 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.6% C: 62.3% G: 30.9% T: 0.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 393 91.9 0 393 4 1 23.0 0 1 6 1 1.4 0 1