This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.60 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 125,363 (4.3%) Reads that were too short: 27,168 (0.9%) Reads written (passing filters): 98,195 (3.4%) Total basepairs processed: 881,011,047 bp Total written (filtered): 24,299,044 bp (2.8%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 125363 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 471 714.6 0 471 7 11921 714.6 0 11921 8 41744 714.6 0 41744 9 85 714.6 0 85 10 32 714.6 0 32 11 31 714.6 0 31 12 34 714.6 0 34 13 31 714.6 0 31 14 26 714.6 0 26 15 26 714.6 0 26 16 31 714.6 0 31 17 33 714.6 0 33 18 96 714.6 0 96 19 30 714.6 0 30 20 70 714.6 0 70 21 38 714.6 0 38 22 56 714.6 0 56 23 26 714.6 0 26 24 22 714.6 0 22 25 22 714.6 0 22 26 30 714.6 0 30 27 41 714.6 0 41 28 27 714.6 0 27 29 6815 714.6 0 6815 30 358 714.6 0 358 31 2474 714.6 0 2474 32 245 714.6 0 245 33 25 714.6 0 25 34 19 714.6 0 19 35 24 714.6 0 24 36 30 714.6 0 30 37 31 714.6 0 31 38 29 714.6 0 29 39 28 714.6 0 28 40 30 714.6 0 30 41 30 714.6 0 30 42 21 714.6 0 21 43 38 714.6 0 38 44 35 714.6 0 35 45 36 714.6 0 36 46 41 714.6 0 41 47 40 714.6 0 40 48 31 714.6 0 31 49 32 714.6 0 32 50 35 714.6 0 35 51 48 714.6 0 48 52 69 714.6 0 69 53 20 714.6 0 20 54 54 714.6 0 54 55 40 714.6 0 40 56 85 714.6 0 85 57 14 714.6 0 14 58 29 714.6 0 29 59 29 714.6 0 29 60 31 714.6 0 31 61 88 714.6 0 88 62 128 714.6 0 128 63 62 714.6 0 62 64 32 714.6 0 32 65 26 714.6 0 26 66 17 714.6 0 17 67 63 714.6 0 63 68 62 714.6 0 62 69 32 714.6 0 32 70 25 714.6 0 25 71 53 714.6 0 53 72 90 714.6 0 90 73 82 714.6 0 82 74 192 714.6 0 192 75 88 714.6 0 88 76 303 714.6 0 303 77 299 714.6 0 299 78 291 714.6 0 291 79 213 714.6 0 213 80 221 714.6 0 221 81 205 714.6 0 205 82 212 714.6 0 212 83 313 714.6 0 313 84 260 714.6 0 260 85 175 714.6 0 175 86 213 714.6 0 213 87 480 714.6 0 480 88 92 714.6 0 92 89 186 714.6 0 186 90 156 714.6 0 156 91 163 714.6 0 163 92 351 714.6 0 351 93 342 714.6 0 342 94 335 714.6 0 335 95 339 714.6 0 339 96 300 714.6 0 300 97 177 714.6 0 177 98 405 714.6 0 405 99 408 714.6 0 408 100 402 714.6 0 402 101 140 714.6 0 140 102 75 714.6 0 75 103 48 714.6 0 48 104 25 714.6 0 25 105 21 714.6 0 21 106 29 714.6 0 29 107 34 714.6 0 34 108 124 714.6 0 124 109 154 714.6 0 154 110 201 714.6 0 201 111 231 714.6 0 231 112 233 714.6 0 233 113 254 714.6 0 254 114 178 714.6 0 178 115 151 714.6 0 151 116 149 714.6 0 149 117 120 714.6 0 120 118 127 714.6 0 127 119 174 714.6 0 174 120 204 714.6 0 204 121 188 714.6 0 188 122 174 714.6 0 174 123 195 714.6 0 195 124 272 714.6 0 272 125 240 714.6 0 240 126 274 714.6 0 274 127 736 714.6 0 736 128 862 714.6 0 862 129 633 714.6 0 633 130 631 714.6 0 631 131 346 714.6 0 346 132 212 714.6 0 212 133 210 714.6 0 210 134 215 714.6 0 215 135 294 714.6 0 294 136 287 714.6 0 287 137 194 714.6 0 194 138 187 714.6 0 187 139 184 714.6 0 184 140 169 714.6 0 169 141 163 714.6 0 163 142 202 714.6 0 202 143 184 714.6 0 184 144 252 714.6 0 252 145 210 714.6 0 210 146 238 714.6 0 238 147 236 714.6 0 236 148 371 714.6 0 371 149 171 714.6 0 171 150 143 714.6 0 143 151 117 714.6 0 117 152 225 714.6 0 225 153 239 714.6 0 239 154 264 714.6 0 264 155 287 714.6 0 287 156 228 714.6 0 228 157 215 714.6 0 215 158 222 714.6 0 222 159 206 714.6 0 206 160 208 714.6 0 208 161 218 714.6 0 218 162 198 714.6 0 198 163 182 714.6 0 182 164 185 714.6 0 185 165 270 714.6 0 270 166 377 714.6 0 377 167 369 714.6 0 369 168 284 714.6 0 284 169 198 714.6 0 198 170 167 714.6 0 167 171 148 714.6 0 148 172 208 714.6 0 208 173 182 714.6 0 182 174 309 714.6 0 309 175 305 714.6 0 305 176 252 714.6 0 252 177 241 714.6 0 241 178 256 714.6 0 256 179 220 714.6 0 220 180 250 714.6 0 250 181 223 714.6 0 223 182 254 714.6 0 254 183 313 714.6 0 313 184 284 714.6 0 284 185 276 714.6 0 276 186 222 714.6 0 222 187 191 714.6 0 191 188 164 714.6 0 164 189 184 714.6 0 184 190 301 714.6 0 301 191 375 714.6 0 375 192 409 714.6 0 409 193 269 714.6 0 269 194 246 714.6 0 246 195 279 714.6 0 279 196 424 714.6 0 424 197 568 714.6 0 568 198 623 714.6 0 623 199 186 714.6 0 186 200 216 714.6 0 216 201 143 714.6 0 143 202 336 714.6 0 336 203 410 714.6 0 410 204 321 714.6 0 321 205 292 714.6 0 292 206 247 714.6 0 247 207 247 714.6 0 247 208 220 714.6 0 220 209 215 714.6 0 215 210 384 714.6 0 384 211 593 714.6 0 593 212 566 714.6 0 566 213 416 714.6 0 416 214 297 714.6 0 297 215 268 714.6 0 268 216 279 714.6 0 279 217 274 714.6 0 274 218 356 714.6 0 356 219 340 714.6 0 340 220 321 714.6 0 321 221 292 714.6 0 292 222 344 714.6 0 344 223 413 714.6 0 413 224 319 714.6 0 319 225 248 714.6 0 248 226 287 714.6 0 287 227 193 714.6 0 193 228 177 714.6 0 177 229 199 714.6 0 199 230 165 714.6 0 165 231 194 714.6 0 194 232 271 714.6 0 271 233 316 714.6 0 316 234 383 714.6 0 383 235 315 714.6 0 315 236 223 714.6 0 223 237 185 714.6 0 185 238 261 714.6 0 261 239 310 714.6 0 310 240 422 714.6 0 422 241 328 714.6 0 328 242 676 714.6 0 676 243 461 714.6 0 461 244 371 714.6 0 371 245 467 714.6 0 467 246 520 714.6 0 520 247 294 714.6 0 294 248 41 714.6 0 41 249 113 714.6 0 113 250 204 714.6 0 204 251 853 714.6 0 853 252 761 714.6 0 761 253 536 714.6 0 536 254 303 714.6 0 303 255 347 714.6 0 347 256 232 714.6 0 232 257 117 714.6 0 117 258 77 714.6 0 77 259 70 714.6 0 70 260 85 714.6 0 85 261 147 714.6 0 147 262 309 714.6 0 309 263 576 714.6 0 576 264 498 714.6 0 498 265 366 714.6 0 366 266 205 714.6 0 205 267 150 714.6 0 150 268 109 714.6 0 109 269 119 714.6 0 119 270 138 714.6 0 138 271 279 714.6 0 279 272 388 714.6 0 388 273 355 714.6 0 355 274 206 714.6 0 206 275 83 714.6 0 83 276 119 714.6 0 119 277 224 714.6 0 224 278 415 714.6 0 415 279 270 714.6 0 270 280 95 714.6 0 95 281 89 714.6 0 89 282 142 714.6 0 142 283 278 714.6 0 278 284 348 714.6 0 348 285 157 714.6 0 157 286 124 714.6 0 124 287 67 714.6 0 67 288 66 714.6 0 66 289 123 714.6 0 123 290 278 714.6 0 278 291 353 714.6 0 353 292 261 714.6 0 261 293 140 714.6 0 140 294 106 714.6 0 106 295 90 714.6 0 90 296 101 714.6 0 101 297 134 714.6 0 134 298 129 714.6 0 129 299 137 714.6 0 137 300 158 714.6 0 158 301 81 714.6 0 81 Finished in 4.49 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 98,195 Reads with adapters: 43,087 (43.9%) Reads that were too short: 39 (0.0%) Reads written (passing filters): 43,048 (43.8%) Total basepairs processed: 24,299,044 bp Total written (filtered): 11,752,105 bp (48.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 43087 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 19 0.0 3 0 0 16 2 1 19 469 0.0 3 0 431 21 12 5 20 42380 0.0 4 41233 967 137 33 10 21 113 0.0 4 13 93 6 1 22 16 0.0 4 5 0 10 1 23 35 0.0 4 0 1 0 24 10 28 1 0.0 4 1 32 3 0.0 4 0 0 0 0 3 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 51 2 0.0 4 0 0 0 0 2 55 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 89 3 0.0 4 0 0 0 1 2 95 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 2 0.0 4 0 0 0 0 2 111 1 0.0 4 0 0 0 1 112 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.48 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 43,048 Reads with adapters: 3,501 (8.1%) Reads written (passing filters): 43,048 (100.0%) Total basepairs processed: 11,752,105 bp Total written (filtered): 11,709,476 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3501 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.5% C: 3.9% G: 91.1% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 672.6 0 2 4 4 168.2 0 4 9 2 0.2 0 0 2 10 253 0.0 1 14 239 11 3198 0.0 1 20 3178 12 16 0.0 1 0 16 13 1 0.0 1 0 1 151 15 0.0 1 11 4 192 1 0.0 1 0 1 209 1 0.0 1 0 1 224 2 0.0 1 2 259 3 0.0 1 3 260 1 0.0 1 1 262 1 0.0 1 1 263 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.