This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.89 s (25 us/read; 2.41 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 69,442 (2.4%) Reads that were too short: 8,258 (0.3%) Reads written (passing filters): 61,184 (2.1%) Total basepairs processed: 881,011,047 bp Total written (filtered): 16,420,187 bp (1.9%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 69442 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 518 714.6 0 518 7 2909 714.6 0 2909 8 686 714.6 0 686 9 42888 714.6 0 42888 10 111 714.6 0 111 11 25 714.6 0 25 12 26 714.6 0 26 13 31 714.6 0 31 14 20 714.6 0 20 15 31 714.6 0 31 16 24 714.6 0 24 17 31 714.6 0 31 18 22 714.6 0 22 19 21 714.6 0 21 20 18 714.6 0 18 21 28 714.6 0 28 22 35 714.6 0 35 23 263 714.6 0 263 24 227 714.6 0 227 25 107 714.6 0 107 26 21 714.6 0 21 27 25 714.6 0 25 28 12 714.6 0 12 29 46 714.6 0 46 30 32 714.6 0 32 31 36 714.6 0 36 32 19 714.6 0 19 33 16 714.6 0 16 34 22 714.6 0 22 35 25 714.6 0 25 36 20 714.6 0 20 37 24 714.6 0 24 38 104 714.6 0 104 39 18 714.6 0 18 40 24 714.6 0 24 41 14 714.6 0 14 42 24 714.6 0 24 43 28 714.6 0 28 44 18 714.6 0 18 45 22 714.6 0 22 46 23 714.6 0 23 47 21 714.6 0 21 48 14 714.6 0 14 49 13 714.6 0 13 50 21 714.6 0 21 51 16 714.6 0 16 52 14 714.6 0 14 53 13 714.6 0 13 54 21 714.6 0 21 55 27 714.6 0 27 56 29 714.6 0 29 57 201 714.6 0 201 58 18 714.6 0 18 59 19 714.6 0 19 60 24 714.6 0 24 61 21 714.6 0 21 62 9 714.6 0 9 63 13 714.6 0 13 64 26 714.6 0 26 65 16 714.6 0 16 66 16 714.6 0 16 67 12 714.6 0 12 68 27 714.6 0 27 69 11 714.6 0 11 70 14 714.6 0 14 71 18 714.6 0 18 72 9 714.6 0 9 73 22 714.6 0 22 74 19 714.6 0 19 75 17 714.6 0 17 76 12 714.6 0 12 77 16 714.6 0 16 78 23 714.6 0 23 79 15 714.6 0 15 80 20 714.6 0 20 81 17 714.6 0 17 82 14 714.6 0 14 83 14 714.6 0 14 84 20 714.6 0 20 85 18 714.6 0 18 86 26 714.6 0 26 87 24 714.6 0 24 88 93 714.6 0 93 89 334 714.6 0 334 90 155 714.6 0 155 91 6 714.6 0 6 92 16 714.6 0 16 93 12 714.6 0 12 94 21 714.6 0 21 95 22 714.6 0 22 96 103 714.6 0 103 97 78 714.6 0 78 98 31 714.6 0 31 99 36 714.6 0 36 100 53 714.6 0 53 101 334 714.6 0 334 102 242 714.6 0 242 103 60 714.6 0 60 104 18 714.6 0 18 105 20 714.6 0 20 106 11 714.6 0 11 107 13 714.6 0 13 108 19 714.6 0 19 109 16 714.6 0 16 110 22 714.6 0 22 111 26 714.6 0 26 112 33 714.6 0 33 113 52 714.6 0 52 114 61 714.6 0 61 115 40 714.6 0 40 116 17 714.6 0 17 117 70 714.6 0 70 118 32 714.6 0 32 119 32 714.6 0 32 120 40 714.6 0 40 121 43 714.6 0 43 122 38 714.6 0 38 123 33 714.6 0 33 124 66 714.6 0 66 125 70 714.6 0 70 126 268 714.6 0 268 127 5144 714.6 0 5144 128 1450 714.6 0 1450 129 1158 714.6 0 1158 130 18 714.6 0 18 131 25 714.6 0 25 132 12 714.6 0 12 133 31 714.6 0 31 134 30 714.6 0 30 135 21 714.6 0 21 136 21 714.6 0 21 137 18 714.6 0 18 138 12 714.6 0 12 139 16 714.6 0 16 140 14 714.6 0 14 141 24 714.6 0 24 142 12 714.6 0 12 143 19 714.6 0 19 144 15 714.6 0 15 145 19 714.6 0 19 146 19 714.6 0 19 147 13 714.6 0 13 148 20 714.6 0 20 149 7 714.6 0 7 150 10 714.6 0 10 151 16 714.6 0 16 152 10 714.6 0 10 153 16 714.6 0 16 154 16 714.6 0 16 155 3 714.6 0 3 156 13 714.6 0 13 157 12 714.6 0 12 158 13 714.6 0 13 159 15 714.6 0 15 160 15 714.6 0 15 161 19 714.6 0 19 162 11 714.6 0 11 163 11 714.6 0 11 164 10 714.6 0 10 165 11 714.6 0 11 166 12 714.6 0 12 167 17 714.6 0 17 168 15 714.6 0 15 169 17 714.6 0 17 170 12 714.6 0 12 171 10 714.6 0 10 172 21 714.6 0 21 173 7 714.6 0 7 174 15 714.6 0 15 175 11 714.6 0 11 176 30 714.6 0 30 177 28 714.6 0 28 178 15 714.6 0 15 179 18 714.6 0 18 180 17 714.6 0 17 181 19 714.6 0 19 182 12 714.6 0 12 183 9 714.6 0 9 184 13 714.6 0 13 185 12 714.6 0 12 186 23 714.6 0 23 187 17 714.6 0 17 188 35 714.6 0 35 189 25 714.6 0 25 190 17 714.6 0 17 191 12 714.6 0 12 192 9 714.6 0 9 193 25 714.6 0 25 194 45 714.6 0 45 195 67 714.6 0 67 196 34 714.6 0 34 197 22 714.6 0 22 198 19 714.6 0 19 199 11 714.6 0 11 200 19 714.6 0 19 201 25 714.6 0 25 202 22 714.6 0 22 203 9 714.6 0 9 204 16 714.6 0 16 205 34 714.6 0 34 206 33 714.6 0 33 207 31 714.6 0 31 208 24 714.6 0 24 209 18 714.6 0 18 210 18 714.6 0 18 211 19 714.6 0 19 212 12 714.6 0 12 213 38 714.6 0 38 214 25 714.6 0 25 215 37 714.6 0 37 216 24 714.6 0 24 217 19 714.6 0 19 218 17 714.6 0 17 219 15 714.6 0 15 220 12 714.6 0 12 221 16 714.6 0 16 222 18 714.6 0 18 223 11 714.6 0 11 224 11 714.6 0 11 225 30 714.6 0 30 226 15 714.6 0 15 227 15 714.6 0 15 228 27 714.6 0 27 229 30 714.6 0 30 230 18 714.6 0 18 231 11 714.6 0 11 232 16 714.6 0 16 233 19 714.6 0 19 234 81 714.6 0 81 235 78 714.6 0 78 236 97 714.6 0 97 237 40 714.6 0 40 238 24 714.6 0 24 239 34 714.6 0 34 240 31 714.6 0 31 241 25 714.6 0 25 242 25 714.6 0 25 243 30 714.6 0 30 244 22 714.6 0 22 245 24 714.6 0 24 246 18 714.6 0 18 247 22 714.6 0 22 248 10 714.6 0 10 249 26 714.6 0 26 250 11 714.6 0 11 251 15 714.6 0 15 252 13 714.6 0 13 253 19 714.6 0 19 254 54 714.6 0 54 255 37 714.6 0 37 256 24 714.6 0 24 257 18 714.6 0 18 258 29 714.6 0 29 259 142 714.6 0 142 260 142 714.6 0 142 261 213 714.6 0 213 262 34 714.6 0 34 263 22 714.6 0 22 264 27 714.6 0 27 265 36 714.6 0 36 266 54 714.6 0 54 267 17 714.6 0 17 268 32 714.6 0 32 269 30 714.6 0 30 270 31 714.6 0 31 271 20 714.6 0 20 272 33 714.6 0 33 273 33 714.6 0 33 274 53 714.6 0 53 275 56 714.6 0 56 276 53 714.6 0 53 277 50 714.6 0 50 278 50 714.6 0 50 279 401 714.6 0 401 280 4427 714.6 0 4427 281 514 714.6 0 514 282 25 714.6 0 25 283 18 714.6 0 18 284 12 714.6 0 12 285 10 714.6 0 10 286 9 714.6 0 9 287 12 714.6 0 12 288 12 714.6 0 12 289 6 714.6 0 6 290 9 714.6 0 9 291 13 714.6 0 13 292 26 714.6 0 26 293 19 714.6 0 19 294 31 714.6 0 31 295 14 714.6 0 14 296 10 714.6 0 10 297 21 714.6 0 21 298 18 714.6 0 18 299 18 714.6 0 18 300 21 714.6 0 21 301 17 714.6 0 17 Finished in 2.71 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 61,184 Reads with adapters: 44,036 (72.0%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 44,025 (72.0%) Total basepairs processed: 16,420,187 bp Total written (filtered): 12,104,491 bp (73.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 44036 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 22 0.0 3 0 0 22 16 383 0.0 3 0 373 6 4 17 43418 0.0 3 42576 824 18 18 113 0.0 3 5 96 11 1 19 30 0.0 3 0 0 24 6 20 30 0.0 3 0 0 0 30 30 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 64 12 0.0 3 0 0 0 12 66 1 0.0 3 0 0 0 1 67 8 0.0 3 0 0 0 8 69 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 166 8 0.0 3 0 0 0 8 170 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.54 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 44,025 Reads with adapters: 1,859 (4.2%) Reads written (passing filters): 44,025 (100.0%) Total basepairs processed: 12,104,491 bp Total written (filtered): 12,098,906 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1859 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.0% C: 7.0% G: 49.4% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1851 687.9 0 1851 4 8 172.0 0 8