This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 79.05 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 246,650 (8.4%) Reads that were too short: 107,569 (3.7%) Reads written (passing filters): 139,081 (4.8%) Total basepairs processed: 881,011,047 bp Total written (filtered): 28,219,291 bp (3.2%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 246650 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1233 714.6 0 1233 7 32205 714.6 0 32205 8 533 714.6 0 533 9 16188 714.6 0 16188 10 29 714.6 0 29 11 20 714.6 0 20 12 14 714.6 0 14 13 9 714.6 0 9 14 7 714.6 0 7 15 14 714.6 0 14 16 12 714.6 0 12 17 12 714.6 0 12 18 16 714.6 0 16 19 5 714.6 0 5 20 9 714.6 0 9 21 19 714.6 0 19 22 23 714.6 0 23 23 20 714.6 0 20 24 13 714.6 0 13 25 10 714.6 0 10 26 15 714.6 0 15 27 10 714.6 0 10 28 13 714.6 0 13 29 17 714.6 0 17 30 13 714.6 0 13 31 17 714.6 0 17 32 9 714.6 0 9 33 14 714.6 0 14 34 14 714.6 0 14 35 27 714.6 0 27 36 24 714.6 0 24 37 14 714.6 0 14 38 23 714.6 0 23 39 26 714.6 0 26 40 19 714.6 0 19 41 44 714.6 0 44 42 35 714.6 0 35 43 16 714.6 0 16 44 10 714.6 0 10 45 18 714.6 0 18 46 12 714.6 0 12 47 18 714.6 0 18 48 19 714.6 0 19 49 12 714.6 0 12 50 12 714.6 0 12 51 12 714.6 0 12 52 12 714.6 0 12 53 12 714.6 0 12 54 80 714.6 0 80 55 30 714.6 0 30 56 9 714.6 0 9 57 14 714.6 0 14 58 9 714.6 0 9 59 14 714.6 0 14 60 16 714.6 0 16 61 22 714.6 0 22 62 12 714.6 0 12 63 14 714.6 0 14 64 15 714.6 0 15 65 18 714.6 0 18 66 26 714.6 0 26 67 30 714.6 0 30 68 35 714.6 0 35 69 176 714.6 0 176 70 1156 714.6 0 1156 71 258 714.6 0 258 72 17 714.6 0 17 73 27 714.6 0 27 74 19 714.6 0 19 75 86 714.6 0 86 76 85 714.6 0 85 77 76 714.6 0 76 78 91 714.6 0 91 79 62 714.6 0 62 80 235 714.6 0 235 81 386 714.6 0 386 82 190 714.6 0 190 83 159 714.6 0 159 84 253 714.6 0 253 85 307 714.6 0 307 86 263 714.6 0 263 87 271 714.6 0 271 88 552 714.6 0 552 89 613 714.6 0 613 90 424 714.6 0 424 91 392 714.6 0 392 92 152 714.6 0 152 93 312 714.6 0 312 94 266 714.6 0 266 95 179 714.6 0 179 96 175 714.6 0 175 97 206 714.6 0 206 98 157 714.6 0 157 99 200 714.6 0 200 100 852 714.6 0 852 101 1237 714.6 0 1237 102 1860 714.6 0 1860 103 907 714.6 0 907 104 469 714.6 0 469 105 238 714.6 0 238 106 156 714.6 0 156 107 99 714.6 0 99 108 37 714.6 0 37 109 60 714.6 0 60 110 154 714.6 0 154 111 285 714.6 0 285 112 359 714.6 0 359 113 273 714.6 0 273 114 352 714.6 0 352 115 340 714.6 0 340 116 433 714.6 0 433 117 605 714.6 0 605 118 768 714.6 0 768 119 515 714.6 0 515 120 343 714.6 0 343 121 361 714.6 0 361 122 493 714.6 0 493 123 926 714.6 0 926 124 1033 714.6 0 1033 125 542 714.6 0 542 126 352 714.6 0 352 127 326 714.6 0 326 128 306 714.6 0 306 129 349 714.6 0 349 130 499 714.6 0 499 131 920 714.6 0 920 132 1518 714.6 0 1518 133 933 714.6 0 933 134 583 714.6 0 583 135 410 714.6 0 410 136 468 714.6 0 468 137 636 714.6 0 636 138 660 714.6 0 660 139 595 714.6 0 595 140 585 714.6 0 585 141 489 714.6 0 489 142 880 714.6 0 880 143 1342 714.6 0 1342 144 1391 714.6 0 1391 145 804 714.6 0 804 146 537 714.6 0 537 147 647 714.6 0 647 148 844 714.6 0 844 149 871 714.6 0 871 150 795 714.6 0 795 151 889 714.6 0 889 152 692 714.6 0 692 153 648 714.6 0 648 154 740 714.6 0 740 155 602 714.6 0 602 156 864 714.6 0 864 157 1085 714.6 0 1085 158 1297 714.6 0 1297 159 1297 714.6 0 1297 160 1662 714.6 0 1662 161 1318 714.6 0 1318 162 1220 714.6 0 1220 163 917 714.6 0 917 164 782 714.6 0 782 165 641 714.6 0 641 166 507 714.6 0 507 167 721 714.6 0 721 168 1145 714.6 0 1145 169 1237 714.6 0 1237 170 1016 714.6 0 1016 171 979 714.6 0 979 172 776 714.6 0 776 173 552 714.6 0 552 174 530 714.6 0 530 175 462 714.6 0 462 176 1655 714.6 0 1655 177 1726 714.6 0 1726 178 1244 714.6 0 1244 179 1036 714.6 0 1036 180 838 714.6 0 838 181 643 714.6 0 643 182 900 714.6 0 900 183 906 714.6 0 906 184 1068 714.6 0 1068 185 941 714.6 0 941 186 918 714.6 0 918 187 1265 714.6 0 1265 188 1361 714.6 0 1361 189 1771 714.6 0 1771 190 978 714.6 0 978 191 563 714.6 0 563 192 462 714.6 0 462 193 475 714.6 0 475 194 849 714.6 0 849 195 800 714.6 0 800 196 1320 714.6 0 1320 197 899 714.6 0 899 198 730 714.6 0 730 199 1021 714.6 0 1021 200 842 714.6 0 842 201 887 714.6 0 887 202 661 714.6 0 661 203 505 714.6 0 505 204 780 714.6 0 780 205 828 714.6 0 828 206 842 714.6 0 842 207 821 714.6 0 821 208 655 714.6 0 655 209 828 714.6 0 828 210 1105 714.6 0 1105 211 1176 714.6 0 1176 212 1090 714.6 0 1090 213 1206 714.6 0 1206 214 1347 714.6 0 1347 215 1271 714.6 0 1271 216 1267 714.6 0 1267 217 1180 714.6 0 1180 218 1096 714.6 0 1096 219 1004 714.6 0 1004 220 1121 714.6 0 1121 221 998 714.6 0 998 222 1463 714.6 0 1463 223 1377 714.6 0 1377 224 1499 714.6 0 1499 225 1326 714.6 0 1326 226 1108 714.6 0 1108 227 1081 714.6 0 1081 228 1133 714.6 0 1133 229 1012 714.6 0 1012 230 1210 714.6 0 1210 231 817 714.6 0 817 232 795 714.6 0 795 233 876 714.6 0 876 234 1000 714.6 0 1000 235 930 714.6 0 930 236 1078 714.6 0 1078 237 1021 714.6 0 1021 238 1292 714.6 0 1292 239 1159 714.6 0 1159 240 1333 714.6 0 1333 241 1188 714.6 0 1188 242 987 714.6 0 987 243 1031 714.6 0 1031 244 1339 714.6 0 1339 245 1526 714.6 0 1526 246 1326 714.6 0 1326 247 1587 714.6 0 1587 248 1213 714.6 0 1213 249 1676 714.6 0 1676 250 917 714.6 0 917 251 937 714.6 0 937 252 530 714.6 0 530 253 889 714.6 0 889 254 1407 714.6 0 1407 255 1500 714.6 0 1500 256 1307 714.6 0 1307 257 1108 714.6 0 1108 258 1012 714.6 0 1012 259 987 714.6 0 987 260 950 714.6 0 950 261 649 714.6 0 649 262 721 714.6 0 721 263 804 714.6 0 804 264 1317 714.6 0 1317 265 1288 714.6 0 1288 266 1140 714.6 0 1140 267 1054 714.6 0 1054 268 912 714.6 0 912 269 1340 714.6 0 1340 270 1078 714.6 0 1078 271 1139 714.6 0 1139 272 912 714.6 0 912 273 971 714.6 0 971 274 1257 714.6 0 1257 275 1326 714.6 0 1326 276 1446 714.6 0 1446 277 1152 714.6 0 1152 278 796 714.6 0 796 279 746 714.6 0 746 280 1231 714.6 0 1231 281 1355 714.6 0 1355 282 1453 714.6 0 1453 283 1311 714.6 0 1311 284 971 714.6 0 971 285 1023 714.6 0 1023 286 991 714.6 0 991 287 1182 714.6 0 1182 288 1295 714.6 0 1295 289 998 714.6 0 998 290 747 714.6 0 747 291 772 714.6 0 772 292 810 714.6 0 810 293 1037 714.6 0 1037 294 1297 714.6 0 1297 295 1074 714.6 0 1074 296 886 714.6 0 886 297 723 714.6 0 723 298 1045 714.6 0 1045 299 904 714.6 0 904 300 1025 714.6 0 1025 301 683 714.6 0 683 Finished in 5.73 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 139,081 Reads with adapters: 33,301 (23.9%) Reads that were too short: 73 (0.1%) Reads written (passing filters): 33,228 (23.9%) Total basepairs processed: 28,219,291 bp Total written (filtered): 9,101,570 bp (32.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 33301 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 13 0.0 3 0 0 11 1 1 19 283 0.0 3 0 268 7 7 1 20 32759 0.0 4 32011 643 68 29 8 21 77 0.0 4 11 62 4 22 13 0.0 4 2 2 8 0 1 23 58 0.0 4 38 2 0 12 6 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 0 1 26 2 0.0 4 0 0 0 0 2 30 2 0.0 4 0 0 0 0 2 31 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 34 3 0.0 4 0 0 0 0 3 37 2 0.0 4 0 0 0 1 1 38 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 44 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 1 56 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 63 2 0.0 4 0 0 0 0 2 64 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 88 2 0.0 4 0 0 0 0 2 90 2 0.0 4 0 0 0 0 2 91 2 0.0 4 0 0 0 0 2 97 2 0.0 4 0 0 0 0 2 100 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 0 1 111 3 0.0 4 0 0 0 2 1 114 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 117 2 0.0 4 0 0 0 0 2 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 1 126 2 0.0 4 0 0 0 0 2 134 2 0.0 4 0 0 0 0 2 135 2 0.0 4 0 0 0 0 2 145 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 179 2 0.0 4 0 0 0 0 2 180 1 0.0 4 0 0 0 0 1 181 2 0.0 4 0 0 0 0 2 182 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 6 0.0 4 0 0 0 0 6 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.13 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 33,228 Reads with adapters: 969 (2.9%) Reads written (passing filters): 33,228 (100.0%) Total basepairs processed: 9,101,570 bp Total written (filtered): 9,084,994 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 969 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.7% C: 7.0% G: 86.7% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 19 519.2 0 19 4 2 129.8 0 2 10 5 0.0 1 1 4 11 824 0.0 1 3 821 12 77 0.0 1 2 75 13 2 0.0 1 0 2 15 1 0.0 1 0 1 137 1 0.0 1 1 152 32 0.0 1 30 2 153 1 0.0 1 1 211 1 0.0 1 1 261 1 0.0 1 1 262 1 0.0 1 1 272 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.