This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.18 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 95,096 (3.2%) Reads that were too short: 25,102 (0.9%) Reads written (passing filters): 69,994 (2.4%) Total basepairs processed: 881,011,047 bp Total written (filtered): 15,826,387 bp (1.8%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 95096 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 142 714.6 0 142 7 694 714.6 0 694 8 32383 714.6 0 32383 9 84 714.6 0 84 10 23 714.6 0 23 11 9 714.6 0 9 12 4 714.6 0 4 13 13 714.6 0 13 14 6 714.6 0 6 15 9 714.6 0 9 16 10 714.6 0 10 17 12 714.6 0 12 18 23 714.6 0 23 19 12 714.6 0 12 20 8 714.6 0 8 21 5 714.6 0 5 22 12 714.6 0 12 23 3 714.6 0 3 24 16 714.6 0 16 25 13 714.6 0 13 26 7 714.6 0 7 27 14 714.6 0 14 28 10 714.6 0 10 29 17 714.6 0 17 30 44 714.6 0 44 31 19 714.6 0 19 32 13 714.6 0 13 33 17 714.6 0 17 34 17 714.6 0 17 35 16 714.6 0 16 36 19 714.6 0 19 37 17 714.6 0 17 38 12 714.6 0 12 39 17 714.6 0 17 40 9 714.6 0 9 41 11 714.6 0 11 42 9 714.6 0 9 43 19 714.6 0 19 44 15 714.6 0 15 45 86 714.6 0 86 46 49 714.6 0 49 47 50 714.6 0 50 48 9 714.6 0 9 49 8 714.6 0 8 50 11 714.6 0 11 51 11 714.6 0 11 52 6 714.6 0 6 53 8 714.6 0 8 54 14 714.6 0 14 55 11 714.6 0 11 56 9 714.6 0 9 57 11 714.6 0 11 58 10 714.6 0 10 59 6 714.6 0 6 60 6 714.6 0 6 61 6 714.6 0 6 62 8 714.6 0 8 63 10 714.6 0 10 64 9 714.6 0 9 65 9 714.6 0 9 66 10 714.6 0 10 67 9 714.6 0 9 68 5 714.6 0 5 69 10 714.6 0 10 70 12 714.6 0 12 71 11 714.6 0 11 72 13 714.6 0 13 73 26 714.6 0 26 74 8 714.6 0 8 75 11 714.6 0 11 76 36 714.6 0 36 77 272 714.6 0 272 78 70 714.6 0 70 79 130 714.6 0 130 80 102 714.6 0 102 81 114 714.6 0 114 82 99 714.6 0 99 83 90 714.6 0 90 84 132 714.6 0 132 85 41 714.6 0 41 86 25 714.6 0 25 87 24 714.6 0 24 88 23 714.6 0 23 89 70 714.6 0 70 90 161 714.6 0 161 91 685 714.6 0 685 92 1868 714.6 0 1868 93 702 714.6 0 702 94 377 714.6 0 377 95 735 714.6 0 735 96 678 714.6 0 678 97 530 714.6 0 530 98 398 714.6 0 398 99 252 714.6 0 252 100 357 714.6 0 357 101 152 714.6 0 152 102 38 714.6 0 38 103 15 714.6 0 15 104 11 714.6 0 11 105 23 714.6 0 23 106 63 714.6 0 63 107 103 714.6 0 103 108 148 714.6 0 148 109 152 714.6 0 152 110 164 714.6 0 164 111 111 714.6 0 111 112 131 714.6 0 131 113 678 714.6 0 678 114 1072 714.6 0 1072 115 731 714.6 0 731 116 531 714.6 0 531 117 272 714.6 0 272 118 196 714.6 0 196 119 223 714.6 0 223 120 223 714.6 0 223 121 194 714.6 0 194 122 242 714.6 0 242 123 252 714.6 0 252 124 197 714.6 0 197 125 230 714.6 0 230 126 312 714.6 0 312 127 172 714.6 0 172 128 320 714.6 0 320 129 502 714.6 0 502 130 408 714.6 0 408 131 428 714.6 0 428 132 291 714.6 0 291 133 245 714.6 0 245 134 327 714.6 0 327 135 517 714.6 0 517 136 793 714.6 0 793 137 453 714.6 0 453 138 346 714.6 0 346 139 466 714.6 0 466 140 296 714.6 0 296 141 227 714.6 0 227 142 261 714.6 0 261 143 332 714.6 0 332 144 246 714.6 0 246 145 232 714.6 0 232 146 140 714.6 0 140 147 168 714.6 0 168 148 183 714.6 0 183 149 218 714.6 0 218 150 215 714.6 0 215 151 135 714.6 0 135 152 245 714.6 0 245 153 296 714.6 0 296 154 276 714.6 0 276 155 345 714.6 0 345 156 255 714.6 0 255 157 272 714.6 0 272 158 221 714.6 0 221 159 171 714.6 0 171 160 136 714.6 0 136 161 139 714.6 0 139 162 222 714.6 0 222 163 309 714.6 0 309 164 412 714.6 0 412 165 281 714.6 0 281 166 233 714.6 0 233 167 296 714.6 0 296 168 351 714.6 0 351 169 328 714.6 0 328 170 254 714.6 0 254 171 197 714.6 0 197 172 235 714.6 0 235 173 273 714.6 0 273 174 227 714.6 0 227 175 221 714.6 0 221 176 178 714.6 0 178 177 318 714.6 0 318 178 380 714.6 0 380 179 295 714.6 0 295 180 283 714.6 0 283 181 270 714.6 0 270 182 309 714.6 0 309 183 283 714.6 0 283 184 327 714.6 0 327 185 319 714.6 0 319 186 200 714.6 0 200 187 186 714.6 0 186 188 280 714.6 0 280 189 362 714.6 0 362 190 451 714.6 0 451 191 384 714.6 0 384 192 333 714.6 0 333 193 218 714.6 0 218 194 202 714.6 0 202 195 272 714.6 0 272 196 265 714.6 0 265 197 368 714.6 0 368 198 184 714.6 0 184 199 124 714.6 0 124 200 213 714.6 0 213 201 194 714.6 0 194 202 333 714.6 0 333 203 727 714.6 0 727 204 468 714.6 0 468 205 311 714.6 0 311 206 184 714.6 0 184 207 178 714.6 0 178 208 290 714.6 0 290 209 358 714.6 0 358 210 534 714.6 0 534 211 454 714.6 0 454 212 326 714.6 0 326 213 256 714.6 0 256 214 249 714.6 0 249 215 361 714.6 0 361 216 435 714.6 0 435 217 345 714.6 0 345 218 211 714.6 0 211 219 173 714.6 0 173 220 200 714.6 0 200 221 256 714.6 0 256 222 219 714.6 0 219 223 266 714.6 0 266 224 334 714.6 0 334 225 834 714.6 0 834 226 636 714.6 0 636 227 860 714.6 0 860 228 238 714.6 0 238 229 169 714.6 0 169 230 201 714.6 0 201 231 217 714.6 0 217 232 279 714.6 0 279 233 297 714.6 0 297 234 385 714.6 0 385 235 496 714.6 0 496 236 476 714.6 0 476 237 406 714.6 0 406 238 170 714.6 0 170 239 145 714.6 0 145 240 172 714.6 0 172 241 147 714.6 0 147 242 208 714.6 0 208 243 318 714.6 0 318 244 199 714.6 0 199 245 213 714.6 0 213 246 152 714.6 0 152 247 95 714.6 0 95 248 76 714.6 0 76 249 107 714.6 0 107 250 190 714.6 0 190 251 203 714.6 0 203 252 630 714.6 0 630 253 339 714.6 0 339 254 176 714.6 0 176 255 150 714.6 0 150 256 119 714.6 0 119 257 112 714.6 0 112 258 105 714.6 0 105 259 187 714.6 0 187 260 249 714.6 0 249 261 236 714.6 0 236 262 215 714.6 0 215 263 252 714.6 0 252 264 224 714.6 0 224 265 175 714.6 0 175 266 95 714.6 0 95 267 67 714.6 0 67 268 95 714.6 0 95 269 145 714.6 0 145 270 271 714.6 0 271 271 325 714.6 0 325 272 218 714.6 0 218 273 195 714.6 0 195 274 156 714.6 0 156 275 160 714.6 0 160 276 130 714.6 0 130 277 164 714.6 0 164 278 154 714.6 0 154 279 91 714.6 0 91 280 106 714.6 0 106 281 133 714.6 0 133 282 133 714.6 0 133 283 199 714.6 0 199 284 196 714.6 0 196 285 129 714.6 0 129 286 113 714.6 0 113 287 99 714.6 0 99 288 122 714.6 0 122 289 220 714.6 0 220 290 332 714.6 0 332 291 325 714.6 0 325 292 160 714.6 0 160 293 147 714.6 0 147 294 107 714.6 0 107 295 133 714.6 0 133 296 238 714.6 0 238 297 430 714.6 0 430 298 360 714.6 0 360 299 298 714.6 0 298 300 241 714.6 0 241 301 89 714.6 0 89 Finished in 2.81 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 69,994 Reads with adapters: 33,166 (47.4%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 33,153 (47.4%) Total basepairs processed: 15,826,387 bp Total written (filtered): 9,146,058 bp (57.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 33166 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 7 0.0 3 0 0 5 2 16 217 0.0 3 0 208 8 1 17 32747 0.0 3 32312 427 7 1 18 98 0.0 3 19 65 13 1 19 23 0.0 3 0 0 15 8 20 20 0.0 3 1 1 0 18 24 1 0.0 3 0 0 0 1 36 2 0.0 3 1 0 0 1 38 5 0.0 3 0 0 3 2 49 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 6 0.0 3 0 0 0 6 81 6 0.0 3 0 0 0 6 83 2 0.0 3 0 0 0 2 84 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 103 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 1 117 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.18 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 33,153 Reads with adapters: 722 (2.2%) Reads written (passing filters): 33,153 (100.0%) Total basepairs processed: 9,146,058 bp Total written (filtered): 9,143,876 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 722 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.6% C: 11.8% G: 61.2% T: 1.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 706 518.0 0 706 4 16 129.5 0 16