This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.12 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 121,792 (4.2%) Reads that were too short: 56,325 (1.9%) Reads written (passing filters): 65,467 (2.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 16,593,598 bp (1.9%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 121792 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 812 714.6 0 812 7 888 714.6 0 888 8 30684 714.6 0 30684 9 11632 714.6 0 11632 10 65 714.6 0 65 11 34 714.6 0 34 12 57 714.6 0 57 13 73 714.6 0 73 14 37 714.6 0 37 15 35 714.6 0 35 16 33 714.6 0 33 17 48 714.6 0 48 18 36 714.6 0 36 19 55 714.6 0 55 20 52 714.6 0 52 21 26 714.6 0 26 22 43 714.6 0 43 23 54 714.6 0 54 24 50 714.6 0 50 25 62 714.6 0 62 26 49 714.6 0 49 27 37 714.6 0 37 28 40 714.6 0 40 29 32 714.6 0 32 30 34 714.6 0 34 31 45 714.6 0 45 32 39 714.6 0 39 33 38 714.6 0 38 34 45 714.6 0 45 35 48 714.6 0 48 36 76 714.6 0 76 37 47 714.6 0 47 38 45 714.6 0 45 39 43 714.6 0 43 40 43 714.6 0 43 41 32 714.6 0 32 42 35 714.6 0 35 43 27 714.6 0 27 44 42 714.6 0 42 45 45 714.6 0 45 46 45 714.6 0 45 47 39 714.6 0 39 48 32 714.6 0 32 49 34 714.6 0 34 50 36 714.6 0 36 51 43 714.6 0 43 52 44 714.6 0 44 53 139 714.6 0 139 54 37 714.6 0 37 55 42 714.6 0 42 56 32 714.6 0 32 57 37 714.6 0 37 58 45 714.6 0 45 59 41 714.6 0 41 60 39 714.6 0 39 61 39 714.6 0 39 62 40 714.6 0 40 63 46 714.6 0 46 64 35 714.6 0 35 65 39 714.6 0 39 66 49 714.6 0 49 67 47 714.6 0 47 68 51 714.6 0 51 69 37 714.6 0 37 70 41 714.6 0 41 71 48 714.6 0 48 72 34 714.6 0 34 73 47 714.6 0 47 74 74 714.6 0 74 75 52 714.6 0 52 76 37 714.6 0 37 77 53 714.6 0 53 78 78 714.6 0 78 79 77 714.6 0 77 80 116 714.6 0 116 81 121 714.6 0 121 82 126 714.6 0 126 83 118 714.6 0 118 84 111 714.6 0 111 85 156 714.6 0 156 86 217 714.6 0 217 87 273 714.6 0 273 88 101 714.6 0 101 89 92 714.6 0 92 90 62 714.6 0 62 91 94 714.6 0 94 92 123 714.6 0 123 93 154 714.6 0 154 94 160 714.6 0 160 95 214 714.6 0 214 96 146 714.6 0 146 97 126 714.6 0 126 98 142 714.6 0 142 99 133 714.6 0 133 100 188 714.6 0 188 101 206 714.6 0 206 102 184 714.6 0 184 103 81 714.6 0 81 104 73 714.6 0 73 105 54 714.6 0 54 106 45 714.6 0 45 107 38 714.6 0 38 108 28 714.6 0 28 109 57 714.6 0 57 110 86 714.6 0 86 111 69 714.6 0 69 112 86 714.6 0 86 113 104 714.6 0 104 114 83 714.6 0 83 115 102 714.6 0 102 116 210 714.6 0 210 117 338 714.6 0 338 118 363 714.6 0 363 119 180 714.6 0 180 120 100 714.6 0 100 121 91 714.6 0 91 122 128 714.6 0 128 123 125 714.6 0 125 124 120 714.6 0 120 125 141 714.6 0 141 126 137 714.6 0 137 127 144 714.6 0 144 128 109 714.6 0 109 129 107 714.6 0 107 130 113 714.6 0 113 131 146 714.6 0 146 132 165 714.6 0 165 133 199 714.6 0 199 134 136 714.6 0 136 135 128 714.6 0 128 136 154 714.6 0 154 137 213 714.6 0 213 138 530 714.6 0 530 139 447 714.6 0 447 140 202 714.6 0 202 141 121 714.6 0 121 142 148 714.6 0 148 143 172 714.6 0 172 144 171 714.6 0 171 145 154 714.6 0 154 146 171 714.6 0 171 147 137 714.6 0 137 148 109 714.6 0 109 149 123 714.6 0 123 150 118 714.6 0 118 151 149 714.6 0 149 152 137 714.6 0 137 153 119 714.6 0 119 154 113 714.6 0 113 155 122 714.6 0 122 156 133 714.6 0 133 157 188 714.6 0 188 158 165 714.6 0 165 159 207 714.6 0 207 160 155 714.6 0 155 161 135 714.6 0 135 162 144 714.6 0 144 163 134 714.6 0 134 164 98 714.6 0 98 165 121 714.6 0 121 166 140 714.6 0 140 167 163 714.6 0 163 168 190 714.6 0 190 169 154 714.6 0 154 170 169 714.6 0 169 171 206 714.6 0 206 172 140 714.6 0 140 173 116 714.6 0 116 174 126 714.6 0 126 175 114 714.6 0 114 176 118 714.6 0 118 177 147 714.6 0 147 178 119 714.6 0 119 179 193 714.6 0 193 180 204 714.6 0 204 181 172 714.6 0 172 182 143 714.6 0 143 183 137 714.6 0 137 184 201 714.6 0 201 185 174 714.6 0 174 186 170 714.6 0 170 187 244 714.6 0 244 188 229 714.6 0 229 189 117 714.6 0 117 190 69 714.6 0 69 191 96 714.6 0 96 192 132 714.6 0 132 193 129 714.6 0 129 194 200 714.6 0 200 195 238 714.6 0 238 196 133 714.6 0 133 197 100 714.6 0 100 198 114 714.6 0 114 199 248 714.6 0 248 200 257 714.6 0 257 201 99 714.6 0 99 202 85 714.6 0 85 203 81 714.6 0 81 204 132 714.6 0 132 205 193 714.6 0 193 206 204 714.6 0 204 207 172 714.6 0 172 208 162 714.6 0 162 209 102 714.6 0 102 210 149 714.6 0 149 211 144 714.6 0 144 212 168 714.6 0 168 213 174 714.6 0 174 214 252 714.6 0 252 215 208 714.6 0 208 216 242 714.6 0 242 217 168 714.6 0 168 218 155 714.6 0 155 219 153 714.6 0 153 220 183 714.6 0 183 221 134 714.6 0 134 222 95 714.6 0 95 223 102 714.6 0 102 224 147 714.6 0 147 225 165 714.6 0 165 226 142 714.6 0 142 227 154 714.6 0 154 228 228 714.6 0 228 229 168 714.6 0 168 230 130 714.6 0 130 231 147 714.6 0 147 232 115 714.6 0 115 233 131 714.6 0 131 234 92 714.6 0 92 235 70 714.6 0 70 236 175 714.6 0 175 237 195 714.6 0 195 238 174 714.6 0 174 239 203 714.6 0 203 240 213 714.6 0 213 241 96 714.6 0 96 242 60 714.6 0 60 243 91 714.6 0 91 244 102 714.6 0 102 245 121 714.6 0 121 246 134 714.6 0 134 247 149 714.6 0 149 248 178 714.6 0 178 249 185 714.6 0 185 250 77 714.6 0 77 251 59 714.6 0 59 252 70 714.6 0 70 253 65 714.6 0 65 254 104 714.6 0 104 255 126 714.6 0 126 256 135 714.6 0 135 257 133 714.6 0 133 258 233 714.6 0 233 259 210 714.6 0 210 260 245 714.6 0 245 261 89 714.6 0 89 262 128 714.6 0 128 263 141 714.6 0 141 264 117 714.6 0 117 265 94 714.6 0 94 266 157 714.6 0 157 267 206 714.6 0 206 268 253 714.6 0 253 269 233 714.6 0 233 270 135 714.6 0 135 271 125 714.6 0 125 272 212 714.6 0 212 273 1403 714.6 0 1403 274 3845 714.6 0 3845 275 3528 714.6 0 3528 276 558 714.6 0 558 277 161 714.6 0 161 278 115 714.6 0 115 279 199 714.6 0 199 280 436 714.6 0 436 281 332 714.6 0 332 282 193 714.6 0 193 283 247 714.6 0 247 284 209 714.6 0 209 285 257 714.6 0 257 286 282 714.6 0 282 287 867 714.6 0 867 288 363 714.6 0 363 289 306 714.6 0 306 290 196 714.6 0 196 291 163 714.6 0 163 292 251 714.6 0 251 293 3086 714.6 0 3086 294 1752 714.6 0 1752 295 5886 714.6 0 5886 296 540 714.6 0 540 297 857 714.6 0 857 298 2753 714.6 0 2753 299 5526 714.6 0 5526 300 8274 714.6 0 8274 301 3170 714.6 0 3170 Finished in 3.21 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 65,467 Reads with adapters: 12,640 (19.3%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 12,619 (19.3%) Total basepairs processed: 16,593,598 bp Total written (filtered): 3,416,562 bp (20.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12640 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 8 0.0 3 0 0 8 19 121 0.0 3 0 112 7 2 20 11951 0.0 4 11205 653 67 18 8 21 35 0.0 4 1 28 2 3 1 22 331 0.0 4 317 10 2 2 23 12 0.0 4 0 2 0 7 3 25 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 1 105 2 0.0 4 0 0 0 1 1 108 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 154 0.0 4 0 0 0 0 154 136 2 0.0 4 0 0 0 0 2 138 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.44 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 12,619 Reads with adapters: 644 (5.1%) Reads written (passing filters): 12,619 (100.0%) Total basepairs processed: 3,416,562 bp Total written (filtered): 3,399,748 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 644 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.9% C: 8.9% G: 81.7% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 49 0.0 0 4 45 10 377 0.0 1 17 360 11 163 0.0 1 1 162 13 2 0.0 1 0 2 150 11 0.0 1 9 2 151 2 0.0 1 2 208 12 0.0 1 11 1 210 2 0.0 1 2 223 21 0.0 1 20 1 224 2 0.0 1 2 256 1 0.0 1 1 259 1 0.0 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.