This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.58 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 74,814 (2.6%) Reads that were too short: 27,478 (0.9%) Reads written (passing filters): 47,336 (1.6%) Total basepairs processed: 881,011,047 bp Total written (filtered): 9,170,625 bp (1.0%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 74814 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 124 714.6 0 124 7 4909 714.6 0 4909 8 4372 714.6 0 4372 9 52 714.6 0 52 10 44 714.6 0 44 11 107 714.6 0 107 12 38 714.6 0 38 13 39 714.6 0 39 14 33 714.6 0 33 15 37 714.6 0 37 16 35 714.6 0 35 17 35 714.6 0 35 18 42 714.6 0 42 19 28 714.6 0 28 20 46 714.6 0 46 21 36 714.6 0 36 22 32 714.6 0 32 23 34 714.6 0 34 24 40 714.6 0 40 25 57 714.6 0 57 26 42 714.6 0 42 27 38 714.6 0 38 28 40 714.6 0 40 29 41 714.6 0 41 30 63 714.6 0 63 31 103 714.6 0 103 32 211 714.6 0 211 33 43 714.6 0 43 34 55 714.6 0 55 35 35 714.6 0 35 36 43 714.6 0 43 37 56 714.6 0 56 38 49 714.6 0 49 39 55 714.6 0 55 40 79 714.6 0 79 41 55 714.6 0 55 42 77 714.6 0 77 43 60 714.6 0 60 44 64 714.6 0 64 45 65 714.6 0 65 46 62 714.6 0 62 47 65 714.6 0 65 48 80 714.6 0 80 49 75 714.6 0 75 50 346 714.6 0 346 51 668 714.6 0 668 52 604 714.6 0 604 53 67 714.6 0 67 54 77 714.6 0 77 55 63 714.6 0 63 56 61 714.6 0 61 57 66 714.6 0 66 58 68 714.6 0 68 59 299 714.6 0 299 60 71 714.6 0 71 61 97 714.6 0 97 62 57 714.6 0 57 63 52 714.6 0 52 64 67 714.6 0 67 65 74 714.6 0 74 66 50 714.6 0 50 67 59 714.6 0 59 68 74 714.6 0 74 69 83 714.6 0 83 70 93 714.6 0 93 71 57 714.6 0 57 72 89 714.6 0 89 73 90 714.6 0 90 74 915 714.6 0 915 75 1122 714.6 0 1122 76 459 714.6 0 459 77 82 714.6 0 82 78 188 714.6 0 188 79 163 714.6 0 163 80 242 714.6 0 242 81 144 714.6 0 144 82 190 714.6 0 190 83 202 714.6 0 202 84 218 714.6 0 218 85 385 714.6 0 385 86 188 714.6 0 188 87 274 714.6 0 274 88 106 714.6 0 106 89 169 714.6 0 169 90 82 714.6 0 82 91 176 714.6 0 176 92 127 714.6 0 127 93 177 714.6 0 177 94 245 714.6 0 245 95 208 714.6 0 208 96 157 714.6 0 157 97 160 714.6 0 160 98 142 714.6 0 142 99 88 714.6 0 88 100 84 714.6 0 84 101 61 714.6 0 61 102 99 714.6 0 99 103 81 714.6 0 81 104 63 714.6 0 63 105 50 714.6 0 50 106 38 714.6 0 38 107 26 714.6 0 26 108 29 714.6 0 29 109 61 714.6 0 61 110 40 714.6 0 40 111 84 714.6 0 84 112 124 714.6 0 124 113 102 714.6 0 102 114 302 714.6 0 302 115 215 714.6 0 215 116 297 714.6 0 297 117 169 714.6 0 169 118 213 714.6 0 213 119 189 714.6 0 189 120 159 714.6 0 159 121 142 714.6 0 142 122 182 714.6 0 182 123 187 714.6 0 187 124 165 714.6 0 165 125 182 714.6 0 182 126 426 714.6 0 426 127 282 714.6 0 282 128 166 714.6 0 166 129 187 714.6 0 187 130 244 714.6 0 244 131 214 714.6 0 214 132 292 714.6 0 292 133 211 714.6 0 211 134 239 714.6 0 239 135 629 714.6 0 629 136 451 714.6 0 451 137 653 714.6 0 653 138 178 714.6 0 178 139 163 714.6 0 163 140 167 714.6 0 167 141 173 714.6 0 173 142 179 714.6 0 179 143 144 714.6 0 144 144 269 714.6 0 269 145 186 714.6 0 186 146 231 714.6 0 231 147 174 714.6 0 174 148 179 714.6 0 179 149 136 714.6 0 136 150 139 714.6 0 139 151 111 714.6 0 111 152 112 714.6 0 112 153 150 714.6 0 150 154 166 714.6 0 166 155 165 714.6 0 165 156 138 714.6 0 138 157 189 714.6 0 189 158 170 714.6 0 170 159 179 714.6 0 179 160 186 714.6 0 186 161 166 714.6 0 166 162 175 714.6 0 175 163 157 714.6 0 157 164 216 714.6 0 216 165 323 714.6 0 323 166 283 714.6 0 283 167 213 714.6 0 213 168 306 714.6 0 306 169 350 714.6 0 350 170 199 714.6 0 199 171 234 714.6 0 234 172 217 714.6 0 217 173 224 714.6 0 224 174 256 714.6 0 256 175 213 714.6 0 213 176 167 714.6 0 167 177 183 714.6 0 183 178 182 714.6 0 182 179 191 714.6 0 191 180 220 714.6 0 220 181 265 714.6 0 265 182 233 714.6 0 233 183 248 714.6 0 248 184 213 714.6 0 213 185 293 714.6 0 293 186 281 714.6 0 281 187 235 714.6 0 235 188 164 714.6 0 164 189 175 714.6 0 175 190 184 714.6 0 184 191 270 714.6 0 270 192 343 714.6 0 343 193 563 714.6 0 563 194 212 714.6 0 212 195 469 714.6 0 469 196 329 714.6 0 329 197 881 714.6 0 881 198 2621 714.6 0 2621 199 650 714.6 0 650 200 1068 714.6 0 1068 201 305 714.6 0 305 202 166 714.6 0 166 203 152 714.6 0 152 204 401 714.6 0 401 205 264 714.6 0 264 206 207 714.6 0 207 207 197 714.6 0 197 208 155 714.6 0 155 209 163 714.6 0 163 210 155 714.6 0 155 211 190 714.6 0 190 212 214 714.6 0 214 213 232 714.6 0 232 214 209 714.6 0 209 215 246 714.6 0 246 216 444 714.6 0 444 217 586 714.6 0 586 218 978 714.6 0 978 219 404 714.6 0 404 220 321 714.6 0 321 221 296 714.6 0 296 222 211 714.6 0 211 223 130 714.6 0 130 224 150 714.6 0 150 225 212 714.6 0 212 226 189 714.6 0 189 227 222 714.6 0 222 228 236 714.6 0 236 229 288 714.6 0 288 230 193 714.6 0 193 231 166 714.6 0 166 232 143 714.6 0 143 233 124 714.6 0 124 234 138 714.6 0 138 235 168 714.6 0 168 236 191 714.6 0 191 237 366 714.6 0 366 238 227 714.6 0 227 239 325 714.6 0 325 240 251 714.6 0 251 241 232 714.6 0 232 242 129 714.6 0 129 243 116 714.6 0 116 244 127 714.6 0 127 245 208 714.6 0 208 246 342 714.6 0 342 247 1369 714.6 0 1369 248 3436 714.6 0 3436 249 1593 714.6 0 1593 250 458 714.6 0 458 251 78 714.6 0 78 252 86 714.6 0 86 253 86 714.6 0 86 254 171 714.6 0 171 255 201 714.6 0 201 256 259 714.6 0 259 257 294 714.6 0 294 258 351 714.6 0 351 259 195 714.6 0 195 260 109 714.6 0 109 261 56 714.6 0 56 262 74 714.6 0 74 263 87 714.6 0 87 264 125 714.6 0 125 265 153 714.6 0 153 266 202 714.6 0 202 267 266 714.6 0 266 268 315 714.6 0 315 269 160 714.6 0 160 270 172 714.6 0 172 271 109 714.6 0 109 272 107 714.6 0 107 273 136 714.6 0 136 274 212 714.6 0 212 275 251 714.6 0 251 276 144 714.6 0 144 277 87 714.6 0 87 278 91 714.6 0 91 279 164 714.6 0 164 280 209 714.6 0 209 281 265 714.6 0 265 282 135 714.6 0 135 283 136 714.6 0 136 284 128 714.6 0 128 285 168 714.6 0 168 286 239 714.6 0 239 287 336 714.6 0 336 288 198 714.6 0 198 289 198 714.6 0 198 290 235 714.6 0 235 291 373 714.6 0 373 292 249 714.6 0 249 293 239 714.6 0 239 294 305 714.6 0 305 295 250 714.6 0 250 296 173 714.6 0 173 297 171 714.6 0 171 298 209 714.6 0 209 299 194 714.6 0 194 300 172 714.6 0 172 301 235 714.6 0 235 Finished in 1.93 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 47,336 Reads with adapters: 4,488 (9.5%) Reads that were too short: 35 (0.1%) Reads written (passing filters): 4,453 (9.4%) Total basepairs processed: 9,170,625 bp Total written (filtered): 1,211,817 bp (13.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4488 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 1 0.0 3 0 0 1 19 46 0.0 3 0 36 3 6 1 20 4350 0.0 4 4225 99 11 7 8 21 21 0.0 4 4 8 0 1 8 23 4 0.0 4 0 1 0 2 1 24 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 86 2 0.0 4 0 0 0 0 2 92 17 0.0 4 0 0 0 0 17 97 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 102 1 0.0 4 0 0 0 1 105 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 2 0.0 4 0 0 0 0 2 111 2 0.0 4 0 0 0 1 1 113 2 0.0 4 0 0 0 0 2 114 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 185 2 0.0 4 0 0 0 0 2 190 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 238 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 4,453 Reads with adapters: 416 (9.3%) Reads written (passing filters): 4,453 (100.0%) Total basepairs processed: 1,211,817 bp Total written (filtered): 1,199,861 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 416 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.6% C: 10.8% G: 83.2% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 69.6 0 8 4 2 17.4 0 2 9 1 0.0 0 0 1 10 33 0.0 1 3 30 11 342 0.0 1 1 341 12 1 0.0 1 0 1 269 22 0.0 1 21 1 270 4 0.0 1 4 271 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.