This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.40 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 61,248 (2.1%) Reads that were too short: 9,063 (0.3%) Reads written (passing filters): 52,185 (1.8%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,768,207 bp (1.0%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 61248 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 117 714.6 0 117 7 1844 714.6 0 1844 8 172 714.6 0 172 9 4076 714.6 0 4076 10 29 714.6 0 29 11 31 714.6 0 31 12 40 714.6 0 40 13 27 714.6 0 27 14 35 714.6 0 35 15 27 714.6 0 27 16 34 714.6 0 34 17 46 714.6 0 46 18 43 714.6 0 43 19 36 714.6 0 36 20 33 714.6 0 33 21 37 714.6 0 37 22 34 714.6 0 34 23 36 714.6 0 36 24 34 714.6 0 34 25 32 714.6 0 32 26 28 714.6 0 28 27 36 714.6 0 36 28 29 714.6 0 29 29 53 714.6 0 53 30 2943 714.6 0 2943 31 367 714.6 0 367 32 41 714.6 0 41 33 142 714.6 0 142 34 103 714.6 0 103 35 100 714.6 0 100 36 44 714.6 0 44 37 33 714.6 0 33 38 31 714.6 0 31 39 30 714.6 0 30 40 26 714.6 0 26 41 35 714.6 0 35 42 27 714.6 0 27 43 36 714.6 0 36 44 31 714.6 0 31 45 28 714.6 0 28 46 53 714.6 0 53 47 40 714.6 0 40 48 21 714.6 0 21 49 31 714.6 0 31 50 53 714.6 0 53 51 23 714.6 0 23 52 31 714.6 0 31 53 41 714.6 0 41 54 32 714.6 0 32 55 195 714.6 0 195 56 40 714.6 0 40 57 46 714.6 0 46 58 106 714.6 0 106 59 33 714.6 0 33 60 59 714.6 0 59 61 28 714.6 0 28 62 25 714.6 0 25 63 29 714.6 0 29 64 32 714.6 0 32 65 30 714.6 0 30 66 30 714.6 0 30 67 15 714.6 0 15 68 33 714.6 0 33 69 26 714.6 0 26 70 21 714.6 0 21 71 28 714.6 0 28 72 156 714.6 0 156 73 29 714.6 0 29 74 29 714.6 0 29 75 21 714.6 0 21 76 24 714.6 0 24 77 31 714.6 0 31 78 28 714.6 0 28 79 30 714.6 0 30 80 22 714.6 0 22 81 19 714.6 0 19 82 33 714.6 0 33 83 31 714.6 0 31 84 90 714.6 0 90 85 37 714.6 0 37 86 26 714.6 0 26 87 21 714.6 0 21 88 31 714.6 0 31 89 28 714.6 0 28 90 34 714.6 0 34 91 36 714.6 0 36 92 45 714.6 0 45 93 53 714.6 0 53 94 80 714.6 0 80 95 44 714.6 0 44 96 68 714.6 0 68 97 86 714.6 0 86 98 51 714.6 0 51 99 55 714.6 0 55 100 58 714.6 0 58 101 50 714.6 0 50 102 39 714.6 0 39 103 36 714.6 0 36 104 54 714.6 0 54 105 57 714.6 0 57 106 445 714.6 0 445 107 1413 714.6 0 1413 108 1663 714.6 0 1663 109 1060 714.6 0 1060 110 26 714.6 0 26 111 31 714.6 0 31 112 35 714.6 0 35 113 25 714.6 0 25 114 26 714.6 0 26 115 28 714.6 0 28 116 45 714.6 0 45 117 42 714.6 0 42 118 20 714.6 0 20 119 34 714.6 0 34 120 36 714.6 0 36 121 82 714.6 0 82 122 54 714.6 0 54 123 53 714.6 0 53 124 41 714.6 0 41 125 35 714.6 0 35 126 29 714.6 0 29 127 132 714.6 0 132 128 395 714.6 0 395 129 415 714.6 0 415 130 87 714.6 0 87 131 78 714.6 0 78 132 38 714.6 0 38 133 42 714.6 0 42 134 66 714.6 0 66 135 86 714.6 0 86 136 237 714.6 0 237 137 165 714.6 0 165 138 116 714.6 0 116 139 37 714.6 0 37 140 75 714.6 0 75 141 105 714.6 0 105 142 121 714.6 0 121 143 273 714.6 0 273 144 681 714.6 0 681 145 389 714.6 0 389 146 60 714.6 0 60 147 106 714.6 0 106 148 40 714.6 0 40 149 97 714.6 0 97 150 53 714.6 0 53 151 44 714.6 0 44 152 60 714.6 0 60 153 339 714.6 0 339 154 157 714.6 0 157 155 130 714.6 0 130 156 83 714.6 0 83 157 40 714.6 0 40 158 34 714.6 0 34 159 84 714.6 0 84 160 64 714.6 0 64 161 39 714.6 0 39 162 41 714.6 0 41 163 32 714.6 0 32 164 45 714.6 0 45 165 76 714.6 0 76 166 68 714.6 0 68 167 81 714.6 0 81 168 68 714.6 0 68 169 158 714.6 0 158 170 146 714.6 0 146 171 68 714.6 0 68 172 36 714.6 0 36 173 51 714.6 0 51 174 95 714.6 0 95 175 132 714.6 0 132 176 87 714.6 0 87 177 46 714.6 0 46 178 51 714.6 0 51 179 91 714.6 0 91 180 77 714.6 0 77 181 120 714.6 0 120 182 161 714.6 0 161 183 277 714.6 0 277 184 318 714.6 0 318 185 902 714.6 0 902 186 20754 714.6 0 20754 187 2532 714.6 0 2532 188 1404 714.6 0 1404 189 47 714.6 0 47 190 28 714.6 0 28 191 53 714.6 0 53 192 48 714.6 0 48 193 32 714.6 0 32 194 53 714.6 0 53 195 39 714.6 0 39 196 22 714.6 0 22 197 39 714.6 0 39 198 32 714.6 0 32 199 34 714.6 0 34 200 58 714.6 0 58 201 33 714.6 0 33 202 56 714.6 0 56 203 78 714.6 0 78 204 191 714.6 0 191 205 198 714.6 0 198 206 72 714.6 0 72 207 51 714.6 0 51 208 55 714.6 0 55 209 77 714.6 0 77 210 66 714.6 0 66 211 42 714.6 0 42 212 50 714.6 0 50 213 62 714.6 0 62 214 58 714.6 0 58 215 63 714.6 0 63 216 111 714.6 0 111 217 688 714.6 0 688 218 1375 714.6 0 1375 219 373 714.6 0 373 220 31 714.6 0 31 221 43 714.6 0 43 222 60 714.6 0 60 223 42 714.6 0 42 224 30 714.6 0 30 225 34 714.6 0 34 226 69 714.6 0 69 227 130 714.6 0 130 228 39 714.6 0 39 229 47 714.6 0 47 230 45 714.6 0 45 231 37 714.6 0 37 232 38 714.6 0 38 233 51 714.6 0 51 234 45 714.6 0 45 235 59 714.6 0 59 236 74 714.6 0 74 237 76 714.6 0 76 238 86 714.6 0 86 239 52 714.6 0 52 240 35 714.6 0 35 241 38 714.6 0 38 242 35 714.6 0 35 243 50 714.6 0 50 244 65 714.6 0 65 245 147 714.6 0 147 246 62 714.6 0 62 247 36 714.6 0 36 248 33 714.6 0 33 249 22 714.6 0 22 250 30 714.6 0 30 251 35 714.6 0 35 252 76 714.6 0 76 253 140 714.6 0 140 254 131 714.6 0 131 255 88 714.6 0 88 256 64 714.6 0 64 257 69 714.6 0 69 258 68 714.6 0 68 259 48 714.6 0 48 260 56 714.6 0 56 261 71 714.6 0 71 262 128 714.6 0 128 263 116 714.6 0 116 264 61 714.6 0 61 265 70 714.6 0 70 266 61 714.6 0 61 267 38 714.6 0 38 268 41 714.6 0 41 269 49 714.6 0 49 270 39 714.6 0 39 271 44 714.6 0 44 272 67 714.6 0 67 273 65 714.6 0 65 274 47 714.6 0 47 275 68 714.6 0 68 276 44 714.6 0 44 277 93 714.6 0 93 278 199 714.6 0 199 279 116 714.6 0 116 280 185 714.6 0 185 281 88 714.6 0 88 282 99 714.6 0 99 283 49 714.6 0 49 284 46 714.6 0 46 285 170 714.6 0 170 286 120 714.6 0 120 287 55 714.6 0 55 288 33 714.6 0 33 289 46 714.6 0 46 290 44 714.6 0 44 291 41 714.6 0 41 292 47 714.6 0 47 293 38 714.6 0 38 294 39 714.6 0 39 295 31 714.6 0 31 296 37 714.6 0 37 297 82 714.6 0 82 298 111 714.6 0 111 299 89 714.6 0 89 300 76 714.6 0 76 301 38 714.6 0 38 Finished in 1.72 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 52,185 Reads with adapters: 4,248 (8.1%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 4,236 (8.1%) Total basepairs processed: 8,768,207 bp Total written (filtered): 1,162,088 bp (13.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4248 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 4 16 41 0.0 3 0 34 0 7 17 4137 0.0 3 4038 96 3 18 13 0.0 3 2 8 1 2 19 16 0.0 3 0 0 6 10 20 2 0.0 3 1 0 0 1 31 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 109 5 0.0 3 0 0 0 5 123 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 132 4 0.0 3 0 0 0 4 134 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 4,236 Reads with adapters: 155 (3.7%) Reads written (passing filters): 4,236 (100.0%) Total basepairs processed: 1,162,088 bp Total written (filtered): 1,161,622 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 155 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.6% C: 11.0% G: 27.1% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 154 66.2 0 154 4 1 16.5 0 1