This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.87 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 54,759 (1.9%) Reads that were too short: 5,499 (0.2%) Reads written (passing filters): 49,260 (1.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 12,742,398 bp (1.4%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 54759 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 296 714.6 0 296 7 727 714.6 0 727 8 22050 714.6 0 22050 9 9298 714.6 0 9298 10 93 714.6 0 93 11 58 714.6 0 58 12 72 714.6 0 72 13 60 714.6 0 60 14 65 714.6 0 65 15 63 714.6 0 63 16 65 714.6 0 65 17 56 714.6 0 56 18 63 714.6 0 63 19 74 714.6 0 74 20 62 714.6 0 62 21 63 714.6 0 63 22 65 714.6 0 65 23 59 714.6 0 59 24 80 714.6 0 80 25 49 714.6 0 49 26 71 714.6 0 71 27 59 714.6 0 59 28 66 714.6 0 66 29 54 714.6 0 54 30 64 714.6 0 64 31 69 714.6 0 69 32 47 714.6 0 47 33 52 714.6 0 52 34 49 714.6 0 49 35 68 714.6 0 68 36 54 714.6 0 54 37 56 714.6 0 56 38 59 714.6 0 59 39 52 714.6 0 52 40 42 714.6 0 42 41 49 714.6 0 49 42 61 714.6 0 61 43 53 714.6 0 53 44 63 714.6 0 63 45 59 714.6 0 59 46 48 714.6 0 48 47 55 714.6 0 55 48 46 714.6 0 46 49 48 714.6 0 48 50 39 714.6 0 39 51 50 714.6 0 50 52 50 714.6 0 50 53 50 714.6 0 50 54 40 714.6 0 40 55 45 714.6 0 45 56 42 714.6 0 42 57 31 714.6 0 31 58 47 714.6 0 47 59 42 714.6 0 42 60 54 714.6 0 54 61 34 714.6 0 34 62 33 714.6 0 33 63 36 714.6 0 36 64 26 714.6 0 26 65 42 714.6 0 42 66 34 714.6 0 34 67 42 714.6 0 42 68 56 714.6 0 56 69 23 714.6 0 23 70 35 714.6 0 35 71 25 714.6 0 25 72 29 714.6 0 29 73 22 714.6 0 22 74 20 714.6 0 20 75 30 714.6 0 30 76 24 714.6 0 24 77 25 714.6 0 25 78 21 714.6 0 21 79 22 714.6 0 22 80 15 714.6 0 15 81 20 714.6 0 20 82 36 714.6 0 36 83 28 714.6 0 28 84 39 714.6 0 39 85 31 714.6 0 31 86 26 714.6 0 26 87 29 714.6 0 29 88 40 714.6 0 40 89 23 714.6 0 23 90 38 714.6 0 38 91 109 714.6 0 109 92 54 714.6 0 54 93 35 714.6 0 35 94 45 714.6 0 45 95 47 714.6 0 47 96 37 714.6 0 37 97 96 714.6 0 96 98 264 714.6 0 264 99 76 714.6 0 76 100 25 714.6 0 25 101 32 714.6 0 32 102 35 714.6 0 35 103 26 714.6 0 26 104 71 714.6 0 71 105 40 714.6 0 40 106 43 714.6 0 43 107 48 714.6 0 48 108 51 714.6 0 51 109 50 714.6 0 50 110 216 714.6 0 216 111 4469 714.6 0 4469 112 204 714.6 0 204 113 219 714.6 0 219 114 51 714.6 0 51 115 143 714.6 0 143 116 197 714.6 0 197 117 229 714.6 0 229 118 1461 714.6 0 1461 119 841 714.6 0 841 120 24 714.6 0 24 121 27 714.6 0 27 122 34 714.6 0 34 123 21 714.6 0 21 124 28 714.6 0 28 125 35 714.6 0 35 126 56 714.6 0 56 127 81 714.6 0 81 128 37 714.6 0 37 129 22 714.6 0 22 130 22 714.6 0 22 131 32 714.6 0 32 132 38 714.6 0 38 133 55 714.6 0 55 134 25 714.6 0 25 135 26 714.6 0 26 136 25 714.6 0 25 137 33 714.6 0 33 138 54 714.6 0 54 139 38 714.6 0 38 140 29 714.6 0 29 141 30 714.6 0 30 142 29 714.6 0 29 143 32 714.6 0 32 144 38 714.6 0 38 145 38 714.6 0 38 146 56 714.6 0 56 147 54 714.6 0 54 148 44 714.6 0 44 149 38 714.6 0 38 150 28 714.6 0 28 151 101 714.6 0 101 152 460 714.6 0 460 153 101 714.6 0 101 154 52 714.6 0 52 155 36 714.6 0 36 156 28 714.6 0 28 157 38 714.6 0 38 158 15 714.6 0 15 159 39 714.6 0 39 160 22 714.6 0 22 161 35 714.6 0 35 162 60 714.6 0 60 163 57 714.6 0 57 164 48 714.6 0 48 165 36 714.6 0 36 166 37 714.6 0 37 167 48 714.6 0 48 168 26 714.6 0 26 169 37 714.6 0 37 170 34 714.6 0 34 171 44 714.6 0 44 172 43 714.6 0 43 173 41 714.6 0 41 174 31 714.6 0 31 175 33 714.6 0 33 176 34 714.6 0 34 177 41 714.6 0 41 178 28 714.6 0 28 179 55 714.6 0 55 180 198 714.6 0 198 181 124 714.6 0 124 182 118 714.6 0 118 183 50 714.6 0 50 184 51 714.6 0 51 185 36 714.6 0 36 186 21 714.6 0 21 187 30 714.6 0 30 188 29 714.6 0 29 189 33 714.6 0 33 190 40 714.6 0 40 191 33 714.6 0 33 192 43 714.6 0 43 193 25 714.6 0 25 194 26 714.6 0 26 195 31 714.6 0 31 196 27 714.6 0 27 197 42 714.6 0 42 198 21 714.6 0 21 199 49 714.6 0 49 200 52 714.6 0 52 201 22 714.6 0 22 202 28 714.6 0 28 203 18 714.6 0 18 204 36 714.6 0 36 205 45 714.6 0 45 206 28 714.6 0 28 207 19 714.6 0 19 208 31 714.6 0 31 209 40 714.6 0 40 210 35 714.6 0 35 211 43 714.6 0 43 212 36 714.6 0 36 213 61 714.6 0 61 214 40 714.6 0 40 215 46 714.6 0 46 216 60 714.6 0 60 217 71 714.6 0 71 218 235 714.6 0 235 219 355 714.6 0 355 220 64 714.6 0 64 221 52 714.6 0 52 222 37 714.6 0 37 223 59 714.6 0 59 224 77 714.6 0 77 225 70 714.6 0 70 226 65 714.6 0 65 227 45 714.6 0 45 228 45 714.6 0 45 229 86 714.6 0 86 230 48 714.6 0 48 231 70 714.6 0 70 232 73 714.6 0 73 233 117 714.6 0 117 234 86 714.6 0 86 235 89 714.6 0 89 236 103 714.6 0 103 237 122 714.6 0 122 238 75 714.6 0 75 239 70 714.6 0 70 240 31 714.6 0 31 241 32 714.6 0 32 242 32 714.6 0 32 243 22 714.6 0 22 244 42 714.6 0 42 245 71 714.6 0 71 246 60 714.6 0 60 247 37 714.6 0 37 248 52 714.6 0 52 249 78 714.6 0 78 250 61 714.6 0 61 251 24 714.6 0 24 252 37 714.6 0 37 253 40 714.6 0 40 254 110 714.6 0 110 255 120 714.6 0 120 256 82 714.6 0 82 257 51 714.6 0 51 258 36 714.6 0 36 259 55 714.6 0 55 260 38 714.6 0 38 261 27 714.6 0 27 262 30 714.6 0 30 263 37 714.6 0 37 264 52 714.6 0 52 265 65 714.6 0 65 266 43 714.6 0 43 267 34 714.6 0 34 268 42 714.6 0 42 269 35 714.6 0 35 270 33 714.6 0 33 271 22 714.6 0 22 272 27 714.6 0 27 273 50 714.6 0 50 274 36 714.6 0 36 275 36 714.6 0 36 276 38 714.6 0 38 277 60 714.6 0 60 278 42 714.6 0 42 279 48 714.6 0 48 280 52 714.6 0 52 281 68 714.6 0 68 282 44 714.6 0 44 283 54 714.6 0 54 284 51 714.6 0 51 285 57 714.6 0 57 286 55 714.6 0 55 287 47 714.6 0 47 288 65 714.6 0 65 289 56 714.6 0 56 290 49 714.6 0 49 291 33 714.6 0 33 292 43 714.6 0 43 293 54 714.6 0 54 294 40 714.6 0 40 295 26 714.6 0 26 296 28 714.6 0 28 297 18 714.6 0 18 298 26 714.6 0 26 299 24 714.6 0 24 300 50 714.6 0 50 301 11 714.6 0 11 Finished in 2.20 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 49,260 Reads with adapters: 22,281 (45.2%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 22,274 (45.2%) Total basepairs processed: 12,742,398 bp Total written (filtered): 6,145,933 bp (48.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22281 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 4 0.0 3 0 0 4 16 180 0.0 3 0 170 9 1 17 21937 0.0 3 21646 284 7 18 67 0.0 3 5 45 15 2 19 30 0.0 3 1 0 13 16 20 30 0.0 3 0 0 0 30 25 1 0.0 3 1 29 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 40 3 0.0 3 1 0 0 2 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 1 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 169 3 0.0 3 0 0 0 3 180 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.78 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 22,274 Reads with adapters: 393 (1.8%) Reads written (passing filters): 22,274 (100.0%) Total basepairs processed: 6,145,933 bp Total written (filtered): 6,144,738 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 393 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.4% C: 20.6% G: 39.4% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 381 348.0 0 381 4 10 87.0 0 10 5 1 21.8 0 1 7 1 1.4 0 1