This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.12 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 257,786 (8.8%) Reads that were too short: 5,556 (0.2%) Reads written (passing filters): 252,230 (8.6%) Total basepairs processed: 881,011,047 bp Total written (filtered): 61,560,261 bp (7.0%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 257786 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 405 714.6 0 405 7 5979 714.6 0 5979 8 19803 714.6 0 19803 9 166 714.6 0 166 10 221 714.6 0 221 11 132 714.6 0 132 12 135 714.6 0 135 13 197 714.6 0 197 14 160 714.6 0 160 15 165 714.6 0 165 16 171 714.6 0 171 17 225 714.6 0 225 18 237 714.6 0 237 19 408 714.6 0 408 20 5855 714.6 0 5855 21 15018 714.6 0 15018 22 10998 714.6 0 10998 23 168 714.6 0 168 24 107 714.6 0 107 25 165 714.6 0 165 26 96 714.6 0 96 27 100 714.6 0 100 28 83 714.6 0 83 29 106 714.6 0 106 30 109 714.6 0 109 31 162 714.6 0 162 32 175 714.6 0 175 33 297 714.6 0 297 34 315 714.6 0 315 35 678 714.6 0 678 36 1552 714.6 0 1552 37 337 714.6 0 337 38 615 714.6 0 615 39 1366 714.6 0 1366 40 692 714.6 0 692 41 580 714.6 0 580 42 957 714.6 0 957 43 865 714.6 0 865 44 870 714.6 0 870 45 750 714.6 0 750 46 1000 714.6 0 1000 47 1052 714.6 0 1052 48 1885 714.6 0 1885 49 27776 714.6 0 27776 50 72070 714.6 0 72070 51 45113 714.6 0 45113 52 240 714.6 0 240 53 74 714.6 0 74 54 39 714.6 0 39 55 50 714.6 0 50 56 60 714.6 0 60 57 71 714.6 0 71 58 39 714.6 0 39 59 37 714.6 0 37 60 37 714.6 0 37 61 54 714.6 0 54 62 74 714.6 0 74 63 73 714.6 0 73 64 122 714.6 0 122 65 102 714.6 0 102 66 41 714.6 0 41 67 43 714.6 0 43 68 45 714.6 0 45 69 37 714.6 0 37 70 38 714.6 0 38 71 43 714.6 0 43 72 38 714.6 0 38 73 59 714.6 0 59 74 43 714.6 0 43 75 43 714.6 0 43 76 36 714.6 0 36 77 40 714.6 0 40 78 42 714.6 0 42 79 52 714.6 0 52 80 46 714.6 0 46 81 54 714.6 0 54 82 48 714.6 0 48 83 44 714.6 0 44 84 61 714.6 0 61 85 61 714.6 0 61 86 59 714.6 0 59 87 53 714.6 0 53 88 46 714.6 0 46 89 38 714.6 0 38 90 49 714.6 0 49 91 24 714.6 0 24 92 34 714.6 0 34 93 33 714.6 0 33 94 33 714.6 0 33 95 31 714.6 0 31 96 31 714.6 0 31 97 27 714.6 0 27 98 49 714.6 0 49 99 36 714.6 0 36 100 25 714.6 0 25 101 29 714.6 0 29 102 48 714.6 0 48 103 39 714.6 0 39 104 30 714.6 0 30 105 25 714.6 0 25 106 55 714.6 0 55 107 32 714.6 0 32 108 20 714.6 0 20 109 31 714.6 0 31 110 24 714.6 0 24 111 36 714.6 0 36 112 46 714.6 0 46 113 34 714.6 0 34 114 23 714.6 0 23 115 32 714.6 0 32 116 33 714.6 0 33 117 103 714.6 0 103 118 111 714.6 0 111 119 83 714.6 0 83 120 47 714.6 0 47 121 43 714.6 0 43 122 43 714.6 0 43 123 45 714.6 0 45 124 34 714.6 0 34 125 45 714.6 0 45 126 40 714.6 0 40 127 45 714.6 0 45 128 175 714.6 0 175 129 38 714.6 0 38 130 62 714.6 0 62 131 60 714.6 0 60 132 41 714.6 0 41 133 33 714.6 0 33 134 27 714.6 0 27 135 38 714.6 0 38 136 26 714.6 0 26 137 35 714.6 0 35 138 43 714.6 0 43 139 65 714.6 0 65 140 40 714.6 0 40 141 43 714.6 0 43 142 42 714.6 0 42 143 33 714.6 0 33 144 48 714.6 0 48 145 40 714.6 0 40 146 64 714.6 0 64 147 90 714.6 0 90 148 43 714.6 0 43 149 39 714.6 0 39 150 51 714.6 0 51 151 56 714.6 0 56 152 53 714.6 0 53 153 43 714.6 0 43 154 27 714.6 0 27 155 46 714.6 0 46 156 28 714.6 0 28 157 42 714.6 0 42 158 56 714.6 0 56 159 48 714.6 0 48 160 39 714.6 0 39 161 39 714.6 0 39 162 24 714.6 0 24 163 26 714.6 0 26 164 44 714.6 0 44 165 40 714.6 0 40 166 41 714.6 0 41 167 40 714.6 0 40 168 46 714.6 0 46 169 43 714.6 0 43 170 56 714.6 0 56 171 73 714.6 0 73 172 61 714.6 0 61 173 50 714.6 0 50 174 47 714.6 0 47 175 54 714.6 0 54 176 72 714.6 0 72 177 84 714.6 0 84 178 109 714.6 0 109 179 147 714.6 0 147 180 187 714.6 0 187 181 444 714.6 0 444 182 5384 714.6 0 5384 183 4220 714.6 0 4220 184 656 714.6 0 656 185 111 714.6 0 111 186 96 714.6 0 96 187 72 714.6 0 72 188 88 714.6 0 88 189 105 714.6 0 105 190 89 714.6 0 89 191 117 714.6 0 117 192 129 714.6 0 129 193 440 714.6 0 440 194 3284 714.6 0 3284 195 1435 714.6 0 1435 196 3651 714.6 0 3651 197 4120 714.6 0 4120 198 725 714.6 0 725 199 45 714.6 0 45 200 47 714.6 0 47 201 29 714.6 0 29 202 31 714.6 0 31 203 44 714.6 0 44 204 27 714.6 0 27 205 57 714.6 0 57 206 40 714.6 0 40 207 35 714.6 0 35 208 35 714.6 0 35 209 39 714.6 0 39 210 43 714.6 0 43 211 48 714.6 0 48 212 35 714.6 0 35 213 57 714.6 0 57 214 97 714.6 0 97 215 87 714.6 0 87 216 71 714.6 0 71 217 54 714.6 0 54 218 57 714.6 0 57 219 63 714.6 0 63 220 83 714.6 0 83 221 86 714.6 0 86 222 64 714.6 0 64 223 60 714.6 0 60 224 47 714.6 0 47 225 39 714.6 0 39 226 42 714.6 0 42 227 50 714.6 0 50 228 54 714.6 0 54 229 47 714.6 0 47 230 42 714.6 0 42 231 42 714.6 0 42 232 40 714.6 0 40 233 38 714.6 0 38 234 59 714.6 0 59 235 37 714.6 0 37 236 81 714.6 0 81 237 49 714.6 0 49 238 55 714.6 0 55 239 67 714.6 0 67 240 94 714.6 0 94 241 64 714.6 0 64 242 39 714.6 0 39 243 22 714.6 0 22 244 39 714.6 0 39 245 35 714.6 0 35 246 73 714.6 0 73 247 55 714.6 0 55 248 45 714.6 0 45 249 52 714.6 0 52 250 54 714.6 0 54 251 129 714.6 0 129 252 95 714.6 0 95 253 39 714.6 0 39 254 26 714.6 0 26 255 51 714.6 0 51 256 54 714.6 0 54 257 66 714.6 0 66 258 53 714.6 0 53 259 34 714.6 0 34 260 60 714.6 0 60 261 73 714.6 0 73 262 37 714.6 0 37 263 88 714.6 0 88 264 114 714.6 0 114 265 117 714.6 0 117 266 160 714.6 0 160 267 72 714.6 0 72 268 52 714.6 0 52 269 32 714.6 0 32 270 35 714.6 0 35 271 50 714.6 0 50 272 34 714.6 0 34 273 38 714.6 0 38 274 39 714.6 0 39 275 46 714.6 0 46 276 31 714.6 0 31 277 32 714.6 0 32 278 63 714.6 0 63 279 49 714.6 0 49 280 48 714.6 0 48 281 23 714.6 0 23 282 43 714.6 0 43 283 44 714.6 0 44 284 44 714.6 0 44 285 42 714.6 0 42 286 41 714.6 0 41 287 42 714.6 0 42 288 80 714.6 0 80 289 128 714.6 0 128 290 163 714.6 0 163 291 39 714.6 0 39 292 50 714.6 0 50 293 53 714.6 0 53 294 51 714.6 0 51 295 29 714.6 0 29 296 31 714.6 0 31 297 25 714.6 0 25 298 35 714.6 0 35 299 26 714.6 0 26 300 83 714.6 0 83 301 93 714.6 0 93 Finished in 10.16 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 252,230 Reads with adapters: 5,559 (2.2%) Reads that were too short: 260 (0.1%) Reads written (passing filters): 5,299 (2.1%) Total basepairs processed: 61,560,261 bp Total written (filtered): 1,391,209 bp (2.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5559 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 25 0.0 3 0 25 17 4737 0.0 3 4692 45 18 10 0.0 3 0 10 19 6 0.0 3 0 0 2 4 20 1 0.0 3 0 0 0 1 21 1 0.0 3 0 0 0 1 27 1 0.0 3 1 45 1 0.0 3 0 0 0 1 46 4 0.0 3 0 0 0 4 51 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 1 64 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 73 8 0.0 3 0 0 0 8 87 44 0.0 3 0 0 0 44 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 92 6 0.0 3 0 0 0 6 93 6 0.0 3 0 0 0 6 94 1 0.0 3 0 0 0 1 97 4 0.0 3 0 0 0 4 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 1 107 1 0.0 3 0 0 0 1 116 3 0.0 3 0 0 0 3 117 1 0.0 3 0 0 0 1 122 35 0.0 3 0 0 0 35 123 30 0.0 3 0 0 0 30 124 17 0.0 3 0 0 0 17 125 182 0.0 3 0 0 2 180 126 7 0.0 3 0 0 0 7 127 3 0.0 3 0 0 0 3 128 35 0.0 3 0 0 0 35 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 133 1 0.0 3 0 0 0 1 134 3 0.0 3 0 0 0 3 137 5 0.0 3 0 0 0 5 138 2 0.0 3 0 0 0 2 139 4 0.0 3 0 0 0 4 140 4 0.0 3 0 0 1 3 141 1 0.0 3 0 0 0 1 142 35 0.0 3 0 0 0 35 145 14 0.0 3 0 0 0 14 146 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 151 3 0.0 3 0 0 0 3 152 1 0.0 3 0 0 0 1 153 4 0.0 3 0 0 0 4 154 15 0.0 3 0 0 0 15 157 4 0.0 3 0 0 0 4 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 5 0.0 3 0 0 0 5 172 22 0.0 3 0 0 0 22 174 3 0.0 3 0 0 0 3 175 1 0.0 3 0 0 0 1 177 3 0.0 3 0 0 0 3 178 1 0.0 3 0 0 0 1 202 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 227 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 234 23 0.0 3 0 0 0 23 235 8 0.0 3 0 0 0 8 236 8 0.0 3 0 0 0 8 237 47 0.0 3 0 0 0 47 238 49 0.0 3 0 0 1 48 239 9 0.0 3 0 0 0 9 240 18 0.0 3 0 0 0 18 241 2 0.0 3 0 0 0 2 242 1 0.0 3 0 0 0 1 243 2 0.0 3 0 0 0 2 244 18 0.0 3 0 0 0 18 245 5 0.0 3 0 0 0 5 246 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 250 4 0.0 3 0 0 0 4 262 1 0.0 3 0 0 0 1 263 3 0.0 3 0 0 0 3 264 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 266 7 0.0 3 0 0 0 7 267 4 0.0 3 0 0 0 4 268 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 5,299 Reads with adapters: 358 (6.8%) Reads written (passing filters): 5,299 (100.0%) Total basepairs processed: 1,391,209 bp Total written (filtered): 1,390,133 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 358 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.4% C: 49.2% G: 42.7% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 356 82.8 0 356 4 2 20.7 0 2