This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.05 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 55,039 (1.9%) Reads that were too short: 6,642 (0.2%) Reads written (passing filters): 48,397 (1.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,943,739 bp (1.0%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 55039 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1029 714.6 0 1029 7 2512 714.6 0 2512 8 4222 714.6 0 4222 9 68 714.6 0 68 10 45 714.6 0 45 11 36 714.6 0 36 12 35 714.6 0 35 13 37 714.6 0 37 14 39 714.6 0 39 15 54 714.6 0 54 16 48 714.6 0 48 17 38 714.6 0 38 18 44 714.6 0 44 19 74 714.6 0 74 20 79 714.6 0 79 21 80 714.6 0 80 22 43 714.6 0 43 23 51 714.6 0 51 24 45 714.6 0 45 25 30 714.6 0 30 26 55 714.6 0 55 27 76 714.6 0 76 28 69 714.6 0 69 29 69 714.6 0 69 30 56 714.6 0 56 31 38 714.6 0 38 32 53 714.6 0 53 33 36 714.6 0 36 34 50 714.6 0 50 35 53 714.6 0 53 36 59 714.6 0 59 37 52 714.6 0 52 38 52 714.6 0 52 39 94 714.6 0 94 40 333 714.6 0 333 41 3693 714.6 0 3693 42 85 714.6 0 85 43 43 714.6 0 43 44 50 714.6 0 50 45 57 714.6 0 57 46 41 714.6 0 41 47 54 714.6 0 54 48 37 714.6 0 37 49 44 714.6 0 44 50 49 714.6 0 49 51 39 714.6 0 39 52 52 714.6 0 52 53 47 714.6 0 47 54 51 714.6 0 51 55 57 714.6 0 57 56 54 714.6 0 54 57 59 714.6 0 59 58 44 714.6 0 44 59 46 714.6 0 46 60 49 714.6 0 49 61 29 714.6 0 29 62 44 714.6 0 44 63 47 714.6 0 47 64 47 714.6 0 47 65 56 714.6 0 56 66 45 714.6 0 45 67 47 714.6 0 47 68 62 714.6 0 62 69 47 714.6 0 47 70 43 714.6 0 43 71 63 714.6 0 63 72 50 714.6 0 50 73 72 714.6 0 72 74 60 714.6 0 60 75 53 714.6 0 53 76 133 714.6 0 133 77 223 714.6 0 223 78 142 714.6 0 142 79 51 714.6 0 51 80 52 714.6 0 52 81 61 714.6 0 61 82 54 714.6 0 54 83 50 714.6 0 50 84 58 714.6 0 58 85 73 714.6 0 73 86 68 714.6 0 68 87 55 714.6 0 55 88 58 714.6 0 58 89 41 714.6 0 41 90 48 714.6 0 48 91 81 714.6 0 81 92 85 714.6 0 85 93 205 714.6 0 205 94 239 714.6 0 239 95 140 714.6 0 140 96 246 714.6 0 246 97 456 714.6 0 456 98 382 714.6 0 382 99 88 714.6 0 88 100 41 714.6 0 41 101 42 714.6 0 42 102 63 714.6 0 63 103 48 714.6 0 48 104 37 714.6 0 37 105 30 714.6 0 30 106 35 714.6 0 35 107 40 714.6 0 40 108 51 714.6 0 51 109 38 714.6 0 38 110 52 714.6 0 52 111 51 714.6 0 51 112 36 714.6 0 36 113 39 714.6 0 39 114 83 714.6 0 83 115 87 714.6 0 87 116 73 714.6 0 73 117 54 714.6 0 54 118 38 714.6 0 38 119 33 714.6 0 33 120 38 714.6 0 38 121 39 714.6 0 39 122 42 714.6 0 42 123 48 714.6 0 48 124 41 714.6 0 41 125 36 714.6 0 36 126 56 714.6 0 56 127 43 714.6 0 43 128 100 714.6 0 100 129 62 714.6 0 62 130 278 714.6 0 278 131 203 714.6 0 203 132 177 714.6 0 177 133 37 714.6 0 37 134 56 714.6 0 56 135 61 714.6 0 61 136 47 714.6 0 47 137 58 714.6 0 58 138 36 714.6 0 36 139 39 714.6 0 39 140 54 714.6 0 54 141 53 714.6 0 53 142 56 714.6 0 56 143 66 714.6 0 66 144 117 714.6 0 117 145 48 714.6 0 48 146 38 714.6 0 38 147 35 714.6 0 35 148 33 714.6 0 33 149 47 714.6 0 47 150 37 714.6 0 37 151 68 714.6 0 68 152 105 714.6 0 105 153 45 714.6 0 45 154 61 714.6 0 61 155 52 714.6 0 52 156 52 714.6 0 52 157 56 714.6 0 56 158 61 714.6 0 61 159 47 714.6 0 47 160 35 714.6 0 35 161 48 714.6 0 48 162 93 714.6 0 93 163 87 714.6 0 87 164 155 714.6 0 155 165 136 714.6 0 136 166 222 714.6 0 222 167 329 714.6 0 329 168 801 714.6 0 801 169 19925 714.6 0 19925 170 2207 714.6 0 2207 171 1032 714.6 0 1032 172 47 714.6 0 47 173 46 714.6 0 46 174 60 714.6 0 60 175 40 714.6 0 40 176 40 714.6 0 40 177 32 714.6 0 32 178 39 714.6 0 39 179 55 714.6 0 55 180 54 714.6 0 54 181 37 714.6 0 37 182 55 714.6 0 55 183 47 714.6 0 47 184 56 714.6 0 56 185 43 714.6 0 43 186 47 714.6 0 47 187 32 714.6 0 32 188 44 714.6 0 44 189 41 714.6 0 41 190 39 714.6 0 39 191 39 714.6 0 39 192 87 714.6 0 87 193 135 714.6 0 135 194 81 714.6 0 81 195 49 714.6 0 49 196 43 714.6 0 43 197 34 714.6 0 34 198 39 714.6 0 39 199 31 714.6 0 31 200 45 714.6 0 45 201 32 714.6 0 32 202 58 714.6 0 58 203 113 714.6 0 113 204 91 714.6 0 91 205 72 714.6 0 72 206 64 714.6 0 64 207 36 714.6 0 36 208 56 714.6 0 56 209 37 714.6 0 37 210 41 714.6 0 41 211 67 714.6 0 67 212 68 714.6 0 68 213 58 714.6 0 58 214 46 714.6 0 46 215 49 714.6 0 49 216 69 714.6 0 69 217 51 714.6 0 51 218 57 714.6 0 57 219 38 714.6 0 38 220 40 714.6 0 40 221 41 714.6 0 41 222 63 714.6 0 63 223 65 714.6 0 65 224 74 714.6 0 74 225 48 714.6 0 48 226 42 714.6 0 42 227 50 714.6 0 50 228 63 714.6 0 63 229 45 714.6 0 45 230 50 714.6 0 50 231 83 714.6 0 83 232 64 714.6 0 64 233 64 714.6 0 64 234 45 714.6 0 45 235 66 714.6 0 66 236 96 714.6 0 96 237 133 714.6 0 133 238 130 714.6 0 130 239 66 714.6 0 66 240 42 714.6 0 42 241 120 714.6 0 120 242 37 714.6 0 37 243 46 714.6 0 46 244 46 714.6 0 46 245 38 714.6 0 38 246 57 714.6 0 57 247 23 714.6 0 23 248 19 714.6 0 19 249 41 714.6 0 41 250 23 714.6 0 23 251 31 714.6 0 31 252 32 714.6 0 32 253 43 714.6 0 43 254 62 714.6 0 62 255 61 714.6 0 61 256 98 714.6 0 98 257 47 714.6 0 47 258 85 714.6 0 85 259 32 714.6 0 32 260 36 714.6 0 36 261 63 714.6 0 63 262 45 714.6 0 45 263 41 714.6 0 41 264 35 714.6 0 35 265 62 714.6 0 62 266 40 714.6 0 40 267 52 714.6 0 52 268 41 714.6 0 41 269 49 714.6 0 49 270 56 714.6 0 56 271 212 714.6 0 212 272 400 714.6 0 400 273 309 714.6 0 309 274 856 714.6 0 856 275 33 714.6 0 33 276 28 714.6 0 28 277 41 714.6 0 41 278 34 714.6 0 34 279 38 714.6 0 38 280 29 714.6 0 29 281 34 714.6 0 34 282 36 714.6 0 36 283 42 714.6 0 42 284 31 714.6 0 31 285 31 714.6 0 31 286 36 714.6 0 36 287 28 714.6 0 28 288 37 714.6 0 37 289 48 714.6 0 48 290 51 714.6 0 51 291 36 714.6 0 36 292 47 714.6 0 47 293 47 714.6 0 47 294 46 714.6 0 46 295 25 714.6 0 25 296 36 714.6 0 36 297 47 714.6 0 47 298 33 714.6 0 33 299 21 714.6 0 21 300 24 714.6 0 24 301 24 714.6 0 24 Finished in 1.90 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 48,397 Reads with adapters: 4,423 (9.1%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 4,414 (9.1%) Total basepairs processed: 8,943,739 bp Total written (filtered): 1,203,231 bp (13.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4423 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 4 0.0 3 0 0 1 3 19 52 0.0 3 0 46 3 2 1 20 4197 0.0 4 4072 89 21 11 4 21 15 0.0 4 4 11 22 3 0.0 4 0 3 23 113 0.0 4 100 5 1 3 4 24 13 0.0 4 0 1 0 0 12 42 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 93 2 0.0 4 0 0 0 1 1 96 2 0.0 4 0 0 0 0 2 106 1 0.0 4 0 0 0 0 1 111 3 0.0 4 0 0 0 2 1 123 1 0.0 4 0 0 0 1 130 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 4,414 Reads with adapters: 2,513 (56.9%) Reads written (passing filters): 4,414 (100.0%) Total basepairs processed: 1,203,231 bp Total written (filtered): 566,575 bp (47.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2513 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 93.1% C: 0.2% G: 6.5% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 10 10 0.0 1 0 10 11 160 0.0 1 0 160 12 1 0.0 1 0 1 270 11 0.0 1 11 271 2252 0.0 1 2163 89 272 47 0.0 1 45 2 273 30 0.0 1 29 1 274 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.