This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.74 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 104,187 (3.6%) Reads that were too short: 24,681 (0.8%) Reads written (passing filters): 79,506 (2.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 20,305,003 bp (2.3%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 104187 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 408 714.6 0 408 7 11967 714.6 0 11967 8 43684 714.6 0 43684 9 83 714.6 0 83 10 18 714.6 0 18 11 14 714.6 0 14 12 29 714.6 0 29 13 24 714.6 0 24 14 22 714.6 0 22 15 21 714.6 0 21 16 28 714.6 0 28 17 22 714.6 0 22 18 29 714.6 0 29 19 31 714.6 0 31 20 49 714.6 0 49 21 76 714.6 0 76 22 62 714.6 0 62 23 16 714.6 0 16 24 17 714.6 0 17 25 19 714.6 0 19 26 17 714.6 0 17 27 36 714.6 0 36 28 19 714.6 0 19 29 18 714.6 0 18 30 17 714.6 0 17 31 14 714.6 0 14 32 22 714.6 0 22 33 28 714.6 0 28 34 22 714.6 0 22 35 21 714.6 0 21 36 29 714.6 0 29 37 36 714.6 0 36 38 23 714.6 0 23 39 24 714.6 0 24 40 21 714.6 0 21 41 27 714.6 0 27 42 23 714.6 0 23 43 11 714.6 0 11 44 29 714.6 0 29 45 29 714.6 0 29 46 40 714.6 0 40 47 32 714.6 0 32 48 28 714.6 0 28 49 34 714.6 0 34 50 33 714.6 0 33 51 30 714.6 0 30 52 61 714.6 0 61 53 25 714.6 0 25 54 45 714.6 0 45 55 59 714.6 0 59 56 113 714.6 0 113 57 19 714.6 0 19 58 24 714.6 0 24 59 24 714.6 0 24 60 30 714.6 0 30 61 44 714.6 0 44 62 46 714.6 0 46 63 25 714.6 0 25 64 19 714.6 0 19 65 33 714.6 0 33 66 28 714.6 0 28 67 87 714.6 0 87 68 50 714.6 0 50 69 25 714.6 0 25 70 17 714.6 0 17 71 33 714.6 0 33 72 39 714.6 0 39 73 25 714.6 0 25 74 29 714.6 0 29 75 25 714.6 0 25 76 47 714.6 0 47 77 99 714.6 0 99 78 151 714.6 0 151 79 66 714.6 0 66 80 53 714.6 0 53 81 54 714.6 0 54 82 25 714.6 0 25 83 70 714.6 0 70 84 27 714.6 0 27 85 20 714.6 0 20 86 32 714.6 0 32 87 174 714.6 0 174 88 27 714.6 0 27 89 36 714.6 0 36 90 54 714.6 0 54 91 52 714.6 0 52 92 98 714.6 0 98 93 282 714.6 0 282 94 270 714.6 0 270 95 162 714.6 0 162 96 115 714.6 0 115 97 104 714.6 0 104 98 300 714.6 0 300 99 656 714.6 0 656 100 1029 714.6 0 1029 101 111 714.6 0 111 102 41 714.6 0 41 103 27 714.6 0 27 104 17 714.6 0 17 105 20 714.6 0 20 106 31 714.6 0 31 107 29 714.6 0 29 108 58 714.6 0 58 109 79 714.6 0 79 110 96 714.6 0 96 111 144 714.6 0 144 112 89 714.6 0 89 113 186 714.6 0 186 114 86 714.6 0 86 115 85 714.6 0 85 116 91 714.6 0 91 117 54 714.6 0 54 118 78 714.6 0 78 119 73 714.6 0 73 120 102 714.6 0 102 121 103 714.6 0 103 122 109 714.6 0 109 123 141 714.6 0 141 124 139 714.6 0 139 125 175 714.6 0 175 126 194 714.6 0 194 127 721 714.6 0 721 128 581 714.6 0 581 129 387 714.6 0 387 130 561 714.6 0 561 131 286 714.6 0 286 132 127 714.6 0 127 133 119 714.6 0 119 134 147 714.6 0 147 135 220 714.6 0 220 136 202 714.6 0 202 137 179 714.6 0 179 138 109 714.6 0 109 139 121 714.6 0 121 140 114 714.6 0 114 141 120 714.6 0 120 142 130 714.6 0 130 143 117 714.6 0 117 144 208 714.6 0 208 145 115 714.6 0 115 146 158 714.6 0 158 147 126 714.6 0 126 148 227 714.6 0 227 149 122 714.6 0 122 150 105 714.6 0 105 151 73 714.6 0 73 152 111 714.6 0 111 153 133 714.6 0 133 154 139 714.6 0 139 155 173 714.6 0 173 156 133 714.6 0 133 157 154 714.6 0 154 158 117 714.6 0 117 159 129 714.6 0 129 160 155 714.6 0 155 161 110 714.6 0 110 162 128 714.6 0 128 163 128 714.6 0 128 164 127 714.6 0 127 165 191 714.6 0 191 166 370 714.6 0 370 167 312 714.6 0 312 168 196 714.6 0 196 169 131 714.6 0 131 170 97 714.6 0 97 171 124 714.6 0 124 172 116 714.6 0 116 173 132 714.6 0 132 174 202 714.6 0 202 175 184 714.6 0 184 176 145 714.6 0 145 177 120 714.6 0 120 178 162 714.6 0 162 179 168 714.6 0 168 180 184 714.6 0 184 181 167 714.6 0 167 182 181 714.6 0 181 183 211 714.6 0 211 184 178 714.6 0 178 185 213 714.6 0 213 186 180 714.6 0 180 187 138 714.6 0 138 188 133 714.6 0 133 189 130 714.6 0 130 190 220 714.6 0 220 191 284 714.6 0 284 192 330 714.6 0 330 193 257 714.6 0 257 194 178 714.6 0 178 195 283 714.6 0 283 196 415 714.6 0 415 197 457 714.6 0 457 198 562 714.6 0 562 199 135 714.6 0 135 200 222 714.6 0 222 201 118 714.6 0 118 202 282 714.6 0 282 203 315 714.6 0 315 204 254 714.6 0 254 205 216 714.6 0 216 206 207 714.6 0 207 207 159 714.6 0 159 208 162 714.6 0 162 209 189 714.6 0 189 210 334 714.6 0 334 211 435 714.6 0 435 212 442 714.6 0 442 213 354 714.6 0 354 214 215 714.6 0 215 215 221 714.6 0 221 216 237 714.6 0 237 217 197 714.6 0 197 218 259 714.6 0 259 219 359 714.6 0 359 220 295 714.6 0 295 221 257 714.6 0 257 222 280 714.6 0 280 223 310 714.6 0 310 224 244 714.6 0 244 225 234 714.6 0 234 226 221 714.6 0 221 227 186 714.6 0 186 228 150 714.6 0 150 229 133 714.6 0 133 230 134 714.6 0 134 231 148 714.6 0 148 232 180 714.6 0 180 233 259 714.6 0 259 234 303 714.6 0 303 235 320 714.6 0 320 236 309 714.6 0 309 237 323 714.6 0 323 238 372 714.6 0 372 239 368 714.6 0 368 240 457 714.6 0 457 241 278 714.6 0 278 242 459 714.6 0 459 243 276 714.6 0 276 244 321 714.6 0 321 245 515 714.6 0 515 246 533 714.6 0 533 247 338 714.6 0 338 248 39 714.6 0 39 249 73 714.6 0 73 250 127 714.6 0 127 251 502 714.6 0 502 252 562 714.6 0 562 253 391 714.6 0 391 254 282 714.6 0 282 255 374 714.6 0 374 256 227 714.6 0 227 257 164 714.6 0 164 258 110 714.6 0 110 259 143 714.6 0 143 260 135 714.6 0 135 261 215 714.6 0 215 262 310 714.6 0 310 263 501 714.6 0 501 264 424 714.6 0 424 265 267 714.6 0 267 266 157 714.6 0 157 267 96 714.6 0 96 268 89 714.6 0 89 269 77 714.6 0 77 270 122 714.6 0 122 271 263 714.6 0 263 272 356 714.6 0 356 273 407 714.6 0 407 274 244 714.6 0 244 275 148 714.6 0 148 276 158 714.6 0 158 277 150 714.6 0 150 278 238 714.6 0 238 279 201 714.6 0 201 280 127 714.6 0 127 281 109 714.6 0 109 282 174 714.6 0 174 283 310 714.6 0 310 284 269 714.6 0 269 285 226 714.6 0 226 286 138 714.6 0 138 287 84 714.6 0 84 288 52 714.6 0 52 289 76 714.6 0 76 290 238 714.6 0 238 291 399 714.6 0 399 292 268 714.6 0 268 293 105 714.6 0 105 294 60 714.6 0 60 295 49 714.6 0 49 296 55 714.6 0 55 297 140 714.6 0 140 298 211 714.6 0 211 299 420 714.6 0 420 300 451 714.6 0 451 301 128 714.6 0 128 Finished in 3.60 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 79,506 Reads with adapters: 10,763 (13.5%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 10,746 (13.5%) Total basepairs processed: 20,305,003 bp Total written (filtered): 2,973,759 bp (14.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10763 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 84 0.0 3 0 83 1 17 10580 0.0 3 10477 102 1 18 32 0.0 3 3 25 1 3 19 17 0.0 3 0 0 9 8 20 12 0.0 3 0 0 0 12 33 2 0.0 3 0 0 0 2 38 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 66 5 0.0 3 0 0 1 4 93 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 190 3 0.0 3 0 0 0 3 192 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 228 2 0.0 3 0 0 0 2 235 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 10,746 Reads with adapters: 677 (6.3%) Reads written (passing filters): 10,746 (100.0%) Total basepairs processed: 2,973,759 bp Total written (filtered): 2,971,724 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 677 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.2% C: 69.3% G: 11.5% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 674 167.9 0 674 4 2 42.0 0 2 5 1 10.5 0 1