This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.42 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 43,400 (1.5%) Reads that were too short: 10,760 (0.4%) Reads written (passing filters): 32,640 (1.1%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,226,564 bp (0.9%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 43400 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2358 714.6 0 2358 7 1924 714.6 0 1924 8 14655 714.6 0 14655 9 93 714.6 0 93 10 81 714.6 0 81 11 36 714.6 0 36 12 53 714.6 0 53 13 37 714.6 0 37 14 58 714.6 0 58 15 46 714.6 0 46 16 52 714.6 0 52 17 209 714.6 0 209 18 59 714.6 0 59 19 55 714.6 0 55 20 56 714.6 0 56 21 32 714.6 0 32 22 28 714.6 0 28 23 46 714.6 0 46 24 42 714.6 0 42 25 44 714.6 0 44 26 46 714.6 0 46 27 58 714.6 0 58 28 44 714.6 0 44 29 58 714.6 0 58 30 83 714.6 0 83 31 40 714.6 0 40 32 34 714.6 0 34 33 80 714.6 0 80 34 35 714.6 0 35 35 37 714.6 0 37 36 55 714.6 0 55 37 38 714.6 0 38 38 45 714.6 0 45 39 50 714.6 0 50 40 48 714.6 0 48 41 35 714.6 0 35 42 99 714.6 0 99 43 54 714.6 0 54 44 17 714.6 0 17 45 36 714.6 0 36 46 50 714.6 0 50 47 55 714.6 0 55 48 55 714.6 0 55 49 57 714.6 0 57 50 33 714.6 0 33 51 55 714.6 0 55 52 42 714.6 0 42 53 88 714.6 0 88 54 46 714.6 0 46 55 38 714.6 0 38 56 49 714.6 0 49 57 63 714.6 0 63 58 46 714.6 0 46 59 41 714.6 0 41 60 70 714.6 0 70 61 68 714.6 0 68 62 56 714.6 0 56 63 59 714.6 0 59 64 37 714.6 0 37 65 46 714.6 0 46 66 40 714.6 0 40 67 58 714.6 0 58 68 45 714.6 0 45 69 34 714.6 0 34 70 38 714.6 0 38 71 49 714.6 0 49 72 46 714.6 0 46 73 286 714.6 0 286 74 326 714.6 0 326 75 620 714.6 0 620 76 40 714.6 0 40 77 235 714.6 0 235 78 329 714.6 0 329 79 301 714.6 0 301 80 30 714.6 0 30 81 32 714.6 0 32 82 41 714.6 0 41 83 31 714.6 0 31 84 45 714.6 0 45 85 43 714.6 0 43 86 63 714.6 0 63 87 37 714.6 0 37 88 39 714.6 0 39 89 38 714.6 0 38 90 49 714.6 0 49 91 35 714.6 0 35 92 59 714.6 0 59 93 64 714.6 0 64 94 58 714.6 0 58 95 39 714.6 0 39 96 43 714.6 0 43 97 46 714.6 0 46 98 43 714.6 0 43 99 48 714.6 0 48 100 38 714.6 0 38 101 26 714.6 0 26 102 24 714.6 0 24 103 35 714.6 0 35 104 32 714.6 0 32 105 32 714.6 0 32 106 65 714.6 0 65 107 224 714.6 0 224 108 33 714.6 0 33 109 33 714.6 0 33 110 44 714.6 0 44 111 28 714.6 0 28 112 41 714.6 0 41 113 45 714.6 0 45 114 55 714.6 0 55 115 65 714.6 0 65 116 47 714.6 0 47 117 47 714.6 0 47 118 44 714.6 0 44 119 33 714.6 0 33 120 65 714.6 0 65 121 55 714.6 0 55 122 39 714.6 0 39 123 27 714.6 0 27 124 48 714.6 0 48 125 57 714.6 0 57 126 39 714.6 0 39 127 106 714.6 0 106 128 405 714.6 0 405 129 249 714.6 0 249 130 117 714.6 0 117 131 59 714.6 0 59 132 53 714.6 0 53 133 78 714.6 0 78 134 190 714.6 0 190 135 163 714.6 0 163 136 257 714.6 0 257 137 131 714.6 0 131 138 54 714.6 0 54 139 65 714.6 0 65 140 66 714.6 0 66 141 31 714.6 0 31 142 41 714.6 0 41 143 48 714.6 0 48 144 76 714.6 0 76 145 56 714.6 0 56 146 281 714.6 0 281 147 134 714.6 0 134 148 408 714.6 0 408 149 39 714.6 0 39 150 78 714.6 0 78 151 36 714.6 0 36 152 42 714.6 0 42 153 38 714.6 0 38 154 33 714.6 0 33 155 95 714.6 0 95 156 93 714.6 0 93 157 60 714.6 0 60 158 41 714.6 0 41 159 81 714.6 0 81 160 64 714.6 0 64 161 52 714.6 0 52 162 30 714.6 0 30 163 41 714.6 0 41 164 55 714.6 0 55 165 69 714.6 0 69 166 81 714.6 0 81 167 50 714.6 0 50 168 59 714.6 0 59 169 44 714.6 0 44 170 54 714.6 0 54 171 39 714.6 0 39 172 29 714.6 0 29 173 36 714.6 0 36 174 42 714.6 0 42 175 34 714.6 0 34 176 37 714.6 0 37 177 41 714.6 0 41 178 41 714.6 0 41 179 51 714.6 0 51 180 57 714.6 0 57 181 60 714.6 0 60 182 53 714.6 0 53 183 46 714.6 0 46 184 112 714.6 0 112 185 125 714.6 0 125 186 136 714.6 0 136 187 45 714.6 0 45 188 39 714.6 0 39 189 24 714.6 0 24 190 36 714.6 0 36 191 41 714.6 0 41 192 64 714.6 0 64 193 45 714.6 0 45 194 36 714.6 0 36 195 72 714.6 0 72 196 98 714.6 0 98 197 64 714.6 0 64 198 50 714.6 0 50 199 40 714.6 0 40 200 36 714.6 0 36 201 47 714.6 0 47 202 31 714.6 0 31 203 30 714.6 0 30 204 58 714.6 0 58 205 61 714.6 0 61 206 37 714.6 0 37 207 45 714.6 0 45 208 42 714.6 0 42 209 53 714.6 0 53 210 42 714.6 0 42 211 65 714.6 0 65 212 64 714.6 0 64 213 49 714.6 0 49 214 69 714.6 0 69 215 95 714.6 0 95 216 84 714.6 0 84 217 84 714.6 0 84 218 56 714.6 0 56 219 69 714.6 0 69 220 65 714.6 0 65 221 63 714.6 0 63 222 38 714.6 0 38 223 63 714.6 0 63 224 73 714.6 0 73 225 78 714.6 0 78 226 70 714.6 0 70 227 65 714.6 0 65 228 50 714.6 0 50 229 47 714.6 0 47 230 46 714.6 0 46 231 62 714.6 0 62 232 54 714.6 0 54 233 78 714.6 0 78 234 120 714.6 0 120 235 249 714.6 0 249 236 3100 714.6 0 3100 237 1670 714.6 0 1670 238 687 714.6 0 687 239 46 714.6 0 46 240 44 714.6 0 44 241 41 714.6 0 41 242 28 714.6 0 28 243 29 714.6 0 29 244 47 714.6 0 47 245 50 714.6 0 50 246 74 714.6 0 74 247 65 714.6 0 65 248 74 714.6 0 74 249 34 714.6 0 34 250 45 714.6 0 45 251 23 714.6 0 23 252 48 714.6 0 48 253 68 714.6 0 68 254 135 714.6 0 135 255 104 714.6 0 104 256 75 714.6 0 75 257 36 714.6 0 36 258 35 714.6 0 35 259 27 714.6 0 27 260 25 714.6 0 25 261 28 714.6 0 28 262 27 714.6 0 27 263 39 714.6 0 39 264 43 714.6 0 43 265 41 714.6 0 41 266 54 714.6 0 54 267 46 714.6 0 46 268 25 714.6 0 25 269 28 714.6 0 28 270 37 714.6 0 37 271 26 714.6 0 26 272 76 714.6 0 76 273 44 714.6 0 44 274 36 714.6 0 36 275 24 714.6 0 24 276 36 714.6 0 36 277 33 714.6 0 33 278 41 714.6 0 41 279 41 714.6 0 41 280 70 714.6 0 70 281 68 714.6 0 68 282 141 714.6 0 141 283 78 714.6 0 78 284 56 714.6 0 56 285 87 714.6 0 87 286 58 714.6 0 58 287 32 714.6 0 32 288 13 714.6 0 13 289 26 714.6 0 26 290 15 714.6 0 15 291 36 714.6 0 36 292 26 714.6 0 26 293 33 714.6 0 33 294 38 714.6 0 38 295 38 714.6 0 38 296 78 714.6 0 78 297 122 714.6 0 122 298 62 714.6 0 62 299 27 714.6 0 27 300 28 714.6 0 28 301 38 714.6 0 38 Finished in 1.65 s (51 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 32,640 Reads with adapters: 17,208 (52.7%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 17,200 (52.7%) Total basepairs processed: 8,226,564 bp Total written (filtered): 4,695,464 bp (57.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 17208 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 3 18 6 0.0 3 0 0 6 19 203 0.0 3 0 192 1 5 5 20 14828 0.0 4 14326 425 56 14 7 21 73 0.0 4 8 63 2 22 2052 0.0 4 1980 60 9 2 1 23 22 0.0 4 1 5 0 14 2 25 5 0.0 4 2 0 0 2 1 70 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 1 76 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 1 117 1 0.0 4 0 0 0 0 1 127 4 0.0 4 0 0 0 0 4 159 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.61 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 17,200 Reads with adapters: 1,732 (10.1%) Reads written (passing filters): 17,200 (100.0%) Total basepairs processed: 4,695,464 bp Total written (filtered): 4,634,552 bp (98.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1732 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.7% C: 3.9% G: 82.9% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 268.8 0 3 4 2 67.2 0 2 8 1 0.3 0 1 9 5 0.1 0 2 3 10 126 0.0 1 6 120 11 1421 0.0 1 10 1411 12 2 0.0 1 0 2 13 1 0.0 1 0 1 209 1 0.0 1 0 1 224 14 0.0 1 12 2 225 1 0.0 1 1 232 2 0.0 1 2 259 121 0.0 1 118 3 260 5 0.0 1 4 1 261 3 0.0 1 3 262 7 0.0 1 5 2 272 17 0.0 1 15 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.