This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.86 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 185,649 (6.3%) Reads that were too short: 81,669 (2.8%) Reads written (passing filters): 103,980 (3.6%) Total basepairs processed: 881,011,047 bp Total written (filtered): 23,205,242 bp (2.6%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 185649 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2154 714.6 0 2154 7 34828 714.6 0 34828 8 385 714.6 0 385 9 14328 714.6 0 14328 10 25 714.6 0 25 11 12 714.6 0 12 12 15 714.6 0 15 13 15 714.6 0 15 14 11 714.6 0 11 15 1129 714.6 0 1129 16 171 714.6 0 171 17 7 714.6 0 7 18 17 714.6 0 17 19 5 714.6 0 5 20 17 714.6 0 17 21 13 714.6 0 13 22 8 714.6 0 8 23 21 714.6 0 21 24 13 714.6 0 13 25 18 714.6 0 18 26 18 714.6 0 18 27 9 714.6 0 9 28 16 714.6 0 16 29 18 714.6 0 18 30 15 714.6 0 15 31 11 714.6 0 11 32 14 714.6 0 14 33 14 714.6 0 14 34 13 714.6 0 13 35 26 714.6 0 26 36 12 714.6 0 12 37 13 714.6 0 13 38 40 714.6 0 40 39 30 714.6 0 30 40 153 714.6 0 153 41 30 714.6 0 30 42 19 714.6 0 19 43 17 714.6 0 17 44 11 714.6 0 11 45 19 714.6 0 19 46 12 714.6 0 12 47 20 714.6 0 20 48 10 714.6 0 10 49 16 714.6 0 16 50 14 714.6 0 14 51 23 714.6 0 23 52 17 714.6 0 17 53 14 714.6 0 14 54 9 714.6 0 9 55 9 714.6 0 9 56 12 714.6 0 12 57 15 714.6 0 15 58 18 714.6 0 18 59 17 714.6 0 17 60 7 714.6 0 7 61 27 714.6 0 27 62 5 714.6 0 5 63 14 714.6 0 14 64 10 714.6 0 10 65 49 714.6 0 49 66 29 714.6 0 29 67 36 714.6 0 36 68 29 714.6 0 29 69 191 714.6 0 191 70 1045 714.6 0 1045 71 288 714.6 0 288 72 10 714.6 0 10 73 16 714.6 0 16 74 14 714.6 0 14 75 13 714.6 0 13 76 9 714.6 0 9 77 16 714.6 0 16 78 10 714.6 0 10 79 26 714.6 0 26 80 42 714.6 0 42 81 169 714.6 0 169 82 95 714.6 0 95 83 62 714.6 0 62 84 72 714.6 0 72 85 88 714.6 0 88 86 68 714.6 0 68 87 50 714.6 0 50 88 55 714.6 0 55 89 109 714.6 0 109 90 47 714.6 0 47 91 66 714.6 0 66 92 33 714.6 0 33 93 61 714.6 0 61 94 75 714.6 0 75 95 72 714.6 0 72 96 86 714.6 0 86 97 111 714.6 0 111 98 77 714.6 0 77 99 89 714.6 0 89 100 398 714.6 0 398 101 590 714.6 0 590 102 1267 714.6 0 1267 103 689 714.6 0 689 104 304 714.6 0 304 105 131 714.6 0 131 106 50 714.6 0 50 107 61 714.6 0 61 108 22 714.6 0 22 109 34 714.6 0 34 110 66 714.6 0 66 111 124 714.6 0 124 112 129 714.6 0 129 113 115 714.6 0 115 114 121 714.6 0 121 115 141 714.6 0 141 116 145 714.6 0 145 117 245 714.6 0 245 118 346 714.6 0 346 119 248 714.6 0 248 120 227 714.6 0 227 121 183 714.6 0 183 122 229 714.6 0 229 123 510 714.6 0 510 124 540 714.6 0 540 125 257 714.6 0 257 126 167 714.6 0 167 127 192 714.6 0 192 128 177 714.6 0 177 129 141 714.6 0 141 130 250 714.6 0 250 131 410 714.6 0 410 132 775 714.6 0 775 133 578 714.6 0 578 134 342 714.6 0 342 135 242 714.6 0 242 136 199 714.6 0 199 137 359 714.6 0 359 138 403 714.6 0 403 139 418 714.6 0 418 140 337 714.6 0 337 141 303 714.6 0 303 142 670 714.6 0 670 143 892 714.6 0 892 144 963 714.6 0 963 145 499 714.6 0 499 146 333 714.6 0 333 147 379 714.6 0 379 148 500 714.6 0 500 149 466 714.6 0 466 150 429 714.6 0 429 151 480 714.6 0 480 152 340 714.6 0 340 153 321 714.6 0 321 154 333 714.6 0 333 155 310 714.6 0 310 156 443 714.6 0 443 157 528 714.6 0 528 158 769 714.6 0 769 159 827 714.6 0 827 160 1220 714.6 0 1220 161 872 714.6 0 872 162 771 714.6 0 771 163 488 714.6 0 488 164 381 714.6 0 381 165 292 714.6 0 292 166 268 714.6 0 268 167 381 714.6 0 381 168 712 714.6 0 712 169 769 714.6 0 769 170 598 714.6 0 598 171 486 714.6 0 486 172 425 714.6 0 425 173 333 714.6 0 333 174 275 714.6 0 275 175 302 714.6 0 302 176 1327 714.6 0 1327 177 1390 714.6 0 1390 178 774 714.6 0 774 179 545 714.6 0 545 180 389 714.6 0 389 181 356 714.6 0 356 182 474 714.6 0 474 183 519 714.6 0 519 184 592 714.6 0 592 185 558 714.6 0 558 186 514 714.6 0 514 187 734 714.6 0 734 188 832 714.6 0 832 189 1199 714.6 0 1199 190 632 714.6 0 632 191 368 714.6 0 368 192 263 714.6 0 263 193 255 714.6 0 255 194 497 714.6 0 497 195 521 714.6 0 521 196 769 714.6 0 769 197 591 714.6 0 591 198 394 714.6 0 394 199 616 714.6 0 616 200 508 714.6 0 508 201 576 714.6 0 576 202 425 714.6 0 425 203 284 714.6 0 284 204 490 714.6 0 490 205 542 714.6 0 542 206 515 714.6 0 515 207 487 714.6 0 487 208 461 714.6 0 461 209 529 714.6 0 529 210 736 714.6 0 736 211 880 714.6 0 880 212 743 714.6 0 743 213 693 714.6 0 693 214 794 714.6 0 794 215 752 714.6 0 752 216 753 714.6 0 753 217 716 714.6 0 716 218 668 714.6 0 668 219 598 714.6 0 598 220 667 714.6 0 667 221 690 714.6 0 690 222 832 714.6 0 832 223 870 714.6 0 870 224 978 714.6 0 978 225 1066 714.6 0 1066 226 727 714.6 0 727 227 735 714.6 0 735 228 726 714.6 0 726 229 682 714.6 0 682 230 933 714.6 0 933 231 602 714.6 0 602 232 544 714.6 0 544 233 619 714.6 0 619 234 681 714.6 0 681 235 614 714.6 0 614 236 698 714.6 0 698 237 669 714.6 0 669 238 880 714.6 0 880 239 1027 714.6 0 1027 240 1393 714.6 0 1393 241 956 714.6 0 956 242 719 714.6 0 719 243 777 714.6 0 777 244 868 714.6 0 868 245 996 714.6 0 996 246 885 714.6 0 885 247 1091 714.6 0 1091 248 950 714.6 0 950 249 1308 714.6 0 1308 250 655 714.6 0 655 251 541 714.6 0 541 252 298 714.6 0 298 253 588 714.6 0 588 254 910 714.6 0 910 255 1064 714.6 0 1064 256 1083 714.6 0 1083 257 970 714.6 0 970 258 881 714.6 0 881 259 858 714.6 0 858 260 826 714.6 0 826 261 647 714.6 0 647 262 679 714.6 0 679 263 1054 714.6 0 1054 264 1656 714.6 0 1656 265 1373 714.6 0 1373 266 977 714.6 0 977 267 829 714.6 0 829 268 662 714.6 0 662 269 1057 714.6 0 1057 270 676 714.6 0 676 271 648 714.6 0 648 272 599 714.6 0 599 273 848 714.6 0 848 274 1226 714.6 0 1226 275 1205 714.6 0 1205 276 1250 714.6 0 1250 277 1184 714.6 0 1184 278 1001 714.6 0 1001 279 680 714.6 0 680 280 1021 714.6 0 1021 281 991 714.6 0 991 282 1341 714.6 0 1341 283 889 714.6 0 889 284 846 714.6 0 846 285 1071 714.6 0 1071 286 902 714.6 0 902 287 1053 714.6 0 1053 288 1129 714.6 0 1129 289 986 714.6 0 986 290 631 714.6 0 631 291 495 714.6 0 495 292 551 714.6 0 551 293 850 714.6 0 850 294 888 714.6 0 888 295 937 714.6 0 937 296 653 714.6 0 653 297 498 714.6 0 498 298 608 714.6 0 608 299 762 714.6 0 762 300 909 714.6 0 909 301 484 714.6 0 484 Finished in 4.29 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 103,980 Reads with adapters: 14,887 (14.3%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 14,862 (14.3%) Total basepairs processed: 23,205,242 bp Total written (filtered): 4,084,527 bp (17.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14887 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 13 0.0 3 0 0 13 16 134 0.0 3 0 130 2 2 17 14463 0.0 3 14159 294 10 18 39 0.0 3 3 32 4 19 11 0.0 3 1 3 3 4 20 182 0.0 3 173 2 0 7 28 1 0.0 3 0 0 0 1 31 1 0.0 3 1 34 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 49 7 0.0 3 0 0 0 7 52 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.55 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 14,862 Reads with adapters: 1,832 (12.3%) Reads written (passing filters): 14,862 (100.0%) Total basepairs processed: 4,084,527 bp Total written (filtered): 4,079,031 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1832 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.6% C: 16.5% G: 48.7% T: 0.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1832 232.2 0 1832