This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.62 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 482,685 (16.5%) Reads that were too short: 197,030 (6.7%) Reads written (passing filters): 285,655 (9.8%) Total basepairs processed: 881,011,047 bp Total written (filtered): 55,785,071 bp (6.3%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 482685 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 931 714.6 0 931 7 27793 714.6 0 27793 8 383 714.6 0 383 9 20570 714.6 0 20570 10 108 714.6 0 108 11 8 714.6 0 8 12 13 714.6 0 13 13 30 714.6 0 30 14 12 714.6 0 12 15 4 714.6 0 4 16 10 714.6 0 10 17 21 714.6 0 21 18 14 714.6 0 14 19 10 714.6 0 10 20 14 714.6 0 14 21 19 714.6 0 19 22 7 714.6 0 7 23 14 714.6 0 14 24 11 714.6 0 11 25 7 714.6 0 7 26 13 714.6 0 13 27 15 714.6 0 15 28 14 714.6 0 14 29 10 714.6 0 10 30 15 714.6 0 15 31 10 714.6 0 10 32 14 714.6 0 14 33 15 714.6 0 15 34 13 714.6 0 13 35 17 714.6 0 17 36 19 714.6 0 19 37 22 714.6 0 22 38 14 714.6 0 14 39 5 714.6 0 5 40 15 714.6 0 15 41 12 714.6 0 12 42 20 714.6 0 20 43 5 714.6 0 5 44 9 714.6 0 9 45 9 714.6 0 9 46 8 714.6 0 8 47 5 714.6 0 5 48 8 714.6 0 8 49 17 714.6 0 17 50 10 714.6 0 10 51 7 714.6 0 7 52 14 714.6 0 14 53 15 714.6 0 15 54 174 714.6 0 174 55 38347 714.6 0 38347 56 549 714.6 0 549 57 35 714.6 0 35 58 13 714.6 0 13 59 14 714.6 0 14 60 13 714.6 0 13 61 84 714.6 0 84 62 29 714.6 0 29 63 14 714.6 0 14 64 17 714.6 0 17 65 49 714.6 0 49 66 49 714.6 0 49 67 30 714.6 0 30 68 9 714.6 0 9 69 25 714.6 0 25 70 77 714.6 0 77 71 69 714.6 0 69 72 97 714.6 0 97 73 172 714.6 0 172 74 344 714.6 0 344 75 382 714.6 0 382 76 1269 714.6 0 1269 77 793 714.6 0 793 78 478 714.6 0 478 79 541 714.6 0 541 80 926 714.6 0 926 81 1664 714.6 0 1664 82 1618 714.6 0 1618 83 1027 714.6 0 1027 84 1025 714.6 0 1025 85 1131 714.6 0 1131 86 2058 714.6 0 2058 87 1309 714.6 0 1309 88 1191 714.6 0 1191 89 1767 714.6 0 1767 90 858 714.6 0 858 91 777 714.6 0 777 92 843 714.6 0 843 93 1199 714.6 0 1199 94 1013 714.6 0 1013 95 1159 714.6 0 1159 96 682 714.6 0 682 97 1347 714.6 0 1347 98 1076 714.6 0 1076 99 1012 714.6 0 1012 100 950 714.6 0 950 101 1123 714.6 0 1123 102 2381 714.6 0 2381 103 1742 714.6 0 1742 104 1327 714.6 0 1327 105 1235 714.6 0 1235 106 1632 714.6 0 1632 107 1844 714.6 0 1844 108 1155 714.6 0 1155 109 2046 714.6 0 2046 110 2101 714.6 0 2101 111 1832 714.6 0 1832 112 1472 714.6 0 1472 113 1852 714.6 0 1852 114 1374 714.6 0 1374 115 1285 714.6 0 1285 116 1015 714.6 0 1015 117 1097 714.6 0 1097 118 2086 714.6 0 2086 119 2225 714.6 0 2225 120 1291 714.6 0 1291 121 894 714.6 0 894 122 1317 714.6 0 1317 123 1601 714.6 0 1601 124 1679 714.6 0 1679 125 2073 714.6 0 2073 126 2190 714.6 0 2190 127 1973 714.6 0 1973 128 1445 714.6 0 1445 129 1146 714.6 0 1146 130 1194 714.6 0 1194 131 1297 714.6 0 1297 132 1665 714.6 0 1665 133 2982 714.6 0 2982 134 2158 714.6 0 2158 135 1368 714.6 0 1368 136 1338 714.6 0 1338 137 1417 714.6 0 1417 138 2546 714.6 0 2546 139 2874 714.6 0 2874 140 2277 714.6 0 2277 141 1309 714.6 0 1309 142 1021 714.6 0 1021 143 1429 714.6 0 1429 144 2552 714.6 0 2552 145 2371 714.6 0 2371 146 1956 714.6 0 1956 147 1602 714.6 0 1602 148 1251 714.6 0 1251 149 1813 714.6 0 1813 150 1576 714.6 0 1576 151 2333 714.6 0 2333 152 2572 714.6 0 2572 153 1933 714.6 0 1933 154 2020 714.6 0 2020 155 1438 714.6 0 1438 156 1896 714.6 0 1896 157 2485 714.6 0 2485 158 1739 714.6 0 1739 159 2164 714.6 0 2164 160 1681 714.6 0 1681 161 1584 714.6 0 1584 162 1334 714.6 0 1334 163 1325 714.6 0 1325 164 2244 714.6 0 2244 165 1924 714.6 0 1924 166 1478 714.6 0 1478 167 1161 714.6 0 1161 168 1197 714.6 0 1197 169 1148 714.6 0 1148 170 1214 714.6 0 1214 171 1397 714.6 0 1397 172 2238 714.6 0 2238 173 1803 714.6 0 1803 174 1244 714.6 0 1244 175 888 714.6 0 888 176 1161 714.6 0 1161 177 1310 714.6 0 1310 178 1163 714.6 0 1163 179 1860 714.6 0 1860 180 2174 714.6 0 2174 181 2087 714.6 0 2087 182 1352 714.6 0 1352 183 1157 714.6 0 1157 184 1483 714.6 0 1483 185 1893 714.6 0 1893 186 1599 714.6 0 1599 187 1300 714.6 0 1300 188 1581 714.6 0 1581 189 1952 714.6 0 1952 190 2103 714.6 0 2103 191 1445 714.6 0 1445 192 1234 714.6 0 1234 193 1171 714.6 0 1171 194 1395 714.6 0 1395 195 1038 714.6 0 1038 196 1544 714.6 0 1544 197 1794 714.6 0 1794 198 1019 714.6 0 1019 199 1821 714.6 0 1821 200 1598 714.6 0 1598 201 1918 714.6 0 1918 202 2123 714.6 0 2123 203 1269 714.6 0 1269 204 1238 714.6 0 1238 205 1732 714.6 0 1732 206 2265 714.6 0 2265 207 1913 714.6 0 1913 208 1701 714.6 0 1701 209 1547 714.6 0 1547 210 2010 714.6 0 2010 211 3462 714.6 0 3462 212 5054 714.6 0 5054 213 3699 714.6 0 3699 214 2623 714.6 0 2623 215 1553 714.6 0 1553 216 1418 714.6 0 1418 217 1333 714.6 0 1333 218 1785 714.6 0 1785 219 2190 714.6 0 2190 220 2225 714.6 0 2225 221 1411 714.6 0 1411 222 1589 714.6 0 1589 223 1798 714.6 0 1798 224 2114 714.6 0 2114 225 1856 714.6 0 1856 226 1606 714.6 0 1606 227 1330 714.6 0 1330 228 1349 714.6 0 1349 229 1529 714.6 0 1529 230 1589 714.6 0 1589 231 1671 714.6 0 1671 232 1421 714.6 0 1421 233 1725 714.6 0 1725 234 1879 714.6 0 1879 235 1556 714.6 0 1556 236 1684 714.6 0 1684 237 1494 714.6 0 1494 238 2430 714.6 0 2430 239 2169 714.6 0 2169 240 2064 714.6 0 2064 241 2310 714.6 0 2310 242 2151 714.6 0 2151 243 1728 714.6 0 1728 244 1368 714.6 0 1368 245 1854 714.6 0 1854 246 1652 714.6 0 1652 247 1577 714.6 0 1577 248 991 714.6 0 991 249 1784 714.6 0 1784 250 1969 714.6 0 1969 251 2500 714.6 0 2500 252 2475 714.6 0 2475 253 1289 714.6 0 1289 254 1416 714.6 0 1416 255 1583 714.6 0 1583 256 1567 714.6 0 1567 257 1540 714.6 0 1540 258 1329 714.6 0 1329 259 1641 714.6 0 1641 260 2069 714.6 0 2069 261 2411 714.6 0 2411 262 2395 714.6 0 2395 263 1615 714.6 0 1615 264 1449 714.6 0 1449 265 1697 714.6 0 1697 266 1704 714.6 0 1704 267 2360 714.6 0 2360 268 1454 714.6 0 1454 269 1740 714.6 0 1740 270 2529 714.6 0 2529 271 3316 714.6 0 3316 272 2971 714.6 0 2971 273 1605 714.6 0 1605 274 2153 714.6 0 2153 275 1632 714.6 0 1632 276 1786 714.6 0 1786 277 2947 714.6 0 2947 278 2450 714.6 0 2450 279 1909 714.6 0 1909 280 1492 714.6 0 1492 281 1458 714.6 0 1458 282 1964 714.6 0 1964 283 2357 714.6 0 2357 284 1784 714.6 0 1784 285 1390 714.6 0 1390 286 1917 714.6 0 1917 287 1682 714.6 0 1682 288 2138 714.6 0 2138 289 2517 714.6 0 2517 290 1673 714.6 0 1673 291 1997 714.6 0 1997 292 2703 714.6 0 2703 293 3450 714.6 0 3450 294 3937 714.6 0 3937 295 2258 714.6 0 2258 296 1912 714.6 0 1912 297 1803 714.6 0 1803 298 2109 714.6 0 2109 299 2372 714.6 0 2372 300 2639 714.6 0 2639 301 1158 714.6 0 1158 Finished in 11.68 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 285,655 Reads with adapters: 28,572 (10.0%) Reads that were too short: 141 (0.0%) Reads written (passing filters): 28,431 (10.0%) Total basepairs processed: 55,785,071 bp Total written (filtered): 7,777,979 bp (13.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28572 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 13 0.0 3 0 0 10 1 2 19 239 0.0 3 0 218 12 7 2 20 27979 0.0 4 27334 548 62 26 9 21 60 0.0 4 10 48 1 0 1 22 2 0.0 4 0 0 1 0 1 23 25 0.0 4 0 0 0 18 7 24 2 0.0 4 0 0 0 1 1 25 2 0.0 4 0 0 0 0 2 26 3 0.0 4 2 0 0 0 1 27 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 29 2 0.0 4 0 0 0 0 2 30 2 0.0 4 0 0 0 0 2 33 3 0.0 4 0 0 0 0 3 35 2 0.0 4 0 0 0 0 2 36 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 44 2 0.0 4 0 0 0 0 2 45 2 0.0 4 0 0 0 0 2 46 1 0.0 4 0 0 0 0 1 47 3 0.0 4 0 0 0 0 3 48 1 0.0 4 0 0 0 0 1 50 2 0.0 4 0 0 0 0 2 51 1 0.0 4 0 0 0 0 1 53 41 0.0 4 0 0 0 33 8 55 4 0.0 4 0 0 0 1 3 56 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 2 0.0 4 0 0 0 0 2 63 2 0.0 4 0 0 0 1 1 64 2 0.0 4 0 0 0 0 2 66 1 0.0 4 0 0 0 0 1 67 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 74 2 0.0 4 0 0 0 0 2 75 2 0.0 4 0 0 0 0 2 76 4 0.0 4 0 0 0 0 4 77 3 0.0 4 0 0 0 0 3 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 3 0.0 4 0 0 0 0 3 82 2 0.0 4 0 0 0 0 2 83 2 0.0 4 0 0 0 0 2 84 3 0.0 4 0 0 0 1 2 85 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 90 3 0.0 4 0 0 0 0 3 92 2 0.0 4 0 0 0 0 2 94 2 0.0 4 0 0 0 0 2 96 2 0.0 4 0 0 0 0 2 97 2 0.0 4 0 0 0 0 2 98 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 103 3 0.0 4 0 0 0 0 3 105 2 0.0 4 0 0 0 0 2 106 3 0.0 4 0 0 0 0 3 107 3 0.0 4 0 0 0 0 3 108 4 0.0 4 0 0 0 0 4 109 1 0.0 4 0 0 0 0 1 110 2 0.0 4 0 0 0 1 1 111 2 0.0 4 0 0 0 0 2 112 2 0.0 4 0 0 0 1 1 113 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 3 0.0 4 0 0 0 0 3 118 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 3 0.0 4 0 0 0 0 3 128 2 0.0 4 0 0 0 0 2 131 2 0.0 4 0 0 0 0 2 133 2 0.0 4 0 0 0 0 2 137 1 0.0 4 0 0 0 0 1 139 2 0.0 4 0 0 0 0 2 140 2 0.0 4 0 0 0 0 2 143 2 0.0 4 0 0 0 0 2 144 1 0.0 4 0 0 0 0 1 146 3 0.0 4 0 0 0 1 2 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 155 3 0.0 4 0 0 0 0 3 157 1 0.0 4 0 0 0 1 160 2 0.0 4 0 0 0 0 2 161 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 171 2 0.0 4 0 0 0 0 2 172 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 1 177 3 0.0 4 0 0 0 0 3 178 3 0.0 4 0 0 0 0 3 180 3 0.0 4 0 0 0 0 3 181 1 0.0 4 0 0 0 0 1 182 2 0.0 4 0 0 0 0 2 186 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 196 2 0.0 4 0 0 0 0 2 198 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 221 2 0.0 4 0 0 0 0 2 227 2 0.0 4 0 0 0 0 2 228 18 0.0 4 0 0 0 0 18 236 1 0.0 4 0 0 0 1 249 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.03 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 28,431 Reads with adapters: 2,660 (9.4%) Reads written (passing filters): 28,431 (100.0%) Total basepairs processed: 7,777,979 bp Total written (filtered): 7,729,917 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2660 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.9% C: 4.4% G: 88.9% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 19 444.2 0 19 4 1 111.1 0 1 9 1 0.1 0 0 1 10 3 0.0 1 0 3 11 2518 0.0 1 14 2504 12 38 0.0 1 0 38 13 2 0.0 1 0 2 152 2 0.0 1 0 2 225 10 0.0 1 8 2 261 64 0.0 1 58 6 262 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.