This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.32 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 102,259 (3.5%) Reads that were too short: 45,771 (1.6%) Reads written (passing filters): 56,488 (1.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 14,916,412 bp (1.7%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 102259 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 758 714.6 0 758 7 803 714.6 0 803 8 28793 714.6 0 28793 9 12829 714.6 0 12829 10 42 714.6 0 42 11 37 714.6 0 37 12 47 714.6 0 47 13 68 714.6 0 68 14 35 714.6 0 35 15 23 714.6 0 23 16 29 714.6 0 29 17 26 714.6 0 26 18 26 714.6 0 26 19 38 714.6 0 38 20 42 714.6 0 42 21 33 714.6 0 33 22 35 714.6 0 35 23 24 714.6 0 24 24 99 714.6 0 99 25 43 714.6 0 43 26 25 714.6 0 25 27 30 714.6 0 30 28 26 714.6 0 26 29 27 714.6 0 27 30 34 714.6 0 34 31 38 714.6 0 38 32 44 714.6 0 44 33 25 714.6 0 25 34 39 714.6 0 39 35 27 714.6 0 27 36 41 714.6 0 41 37 34 714.6 0 34 38 37 714.6 0 37 39 33 714.6 0 33 40 25 714.6 0 25 41 25 714.6 0 25 42 36 714.6 0 36 43 33 714.6 0 33 44 38 714.6 0 38 45 25 714.6 0 25 46 39 714.6 0 39 47 45 714.6 0 45 48 23 714.6 0 23 49 24 714.6 0 24 50 38 714.6 0 38 51 22 714.6 0 22 52 32 714.6 0 32 53 26 714.6 0 26 54 33 714.6 0 33 55 21 714.6 0 21 56 26 714.6 0 26 57 23 714.6 0 23 58 27 714.6 0 27 59 23 714.6 0 23 60 29 714.6 0 29 61 35 714.6 0 35 62 38 714.6 0 38 63 27 714.6 0 27 64 25 714.6 0 25 65 38 714.6 0 38 66 46 714.6 0 46 67 33 714.6 0 33 68 32 714.6 0 32 69 27 714.6 0 27 70 36 714.6 0 36 71 33 714.6 0 33 72 32 714.6 0 32 73 34 714.6 0 34 74 29 714.6 0 29 75 46 714.6 0 46 76 30 714.6 0 30 77 22 714.6 0 22 78 34 714.6 0 34 79 30 714.6 0 30 80 33 714.6 0 33 81 33 714.6 0 33 82 57 714.6 0 57 83 32 714.6 0 32 84 31 714.6 0 31 85 33 714.6 0 33 86 50 714.6 0 50 87 27 714.6 0 27 88 33 714.6 0 33 89 23 714.6 0 23 90 24 714.6 0 24 91 35 714.6 0 35 92 33 714.6 0 33 93 36 714.6 0 36 94 43 714.6 0 43 95 56 714.6 0 56 96 87 714.6 0 87 97 86 714.6 0 86 98 73 714.6 0 73 99 69 714.6 0 69 100 77 714.6 0 77 101 125 714.6 0 125 102 192 714.6 0 192 103 87 714.6 0 87 104 52 714.6 0 52 105 42 714.6 0 42 106 34 714.6 0 34 107 33 714.6 0 33 108 24 714.6 0 24 109 31 714.6 0 31 110 46 714.6 0 46 111 38 714.6 0 38 112 33 714.6 0 33 113 41 714.6 0 41 114 43 714.6 0 43 115 43 714.6 0 43 116 116 714.6 0 116 117 187 714.6 0 187 118 260 714.6 0 260 119 88 714.6 0 88 120 59 714.6 0 59 121 51 714.6 0 51 122 67 714.6 0 67 123 67 714.6 0 67 124 60 714.6 0 60 125 69 714.6 0 69 126 76 714.6 0 76 127 60 714.6 0 60 128 59 714.6 0 59 129 60 714.6 0 60 130 82 714.6 0 82 131 82 714.6 0 82 132 104 714.6 0 104 133 145 714.6 0 145 134 89 714.6 0 89 135 85 714.6 0 85 136 85 714.6 0 85 137 145 714.6 0 145 138 388 714.6 0 388 139 341 714.6 0 341 140 156 714.6 0 156 141 67 714.6 0 67 142 92 714.6 0 92 143 133 714.6 0 133 144 110 714.6 0 110 145 93 714.6 0 93 146 96 714.6 0 96 147 90 714.6 0 90 148 64 714.6 0 64 149 64 714.6 0 64 150 66 714.6 0 66 151 107 714.6 0 107 152 84 714.6 0 84 153 66 714.6 0 66 154 45 714.6 0 45 155 82 714.6 0 82 156 95 714.6 0 95 157 126 714.6 0 126 158 129 714.6 0 129 159 120 714.6 0 120 160 108 714.6 0 108 161 88 714.6 0 88 162 73 714.6 0 73 163 57 714.6 0 57 164 70 714.6 0 70 165 87 714.6 0 87 166 75 714.6 0 75 167 101 714.6 0 101 168 91 714.6 0 91 169 78 714.6 0 78 170 117 714.6 0 117 171 126 714.6 0 126 172 90 714.6 0 90 173 81 714.6 0 81 174 75 714.6 0 75 175 89 714.6 0 89 176 84 714.6 0 84 177 110 714.6 0 110 178 83 714.6 0 83 179 154 714.6 0 154 180 151 714.6 0 151 181 119 714.6 0 119 182 83 714.6 0 83 183 103 714.6 0 103 184 162 714.6 0 162 185 104 714.6 0 104 186 105 714.6 0 105 187 208 714.6 0 208 188 165 714.6 0 165 189 85 714.6 0 85 190 52 714.6 0 52 191 64 714.6 0 64 192 107 714.6 0 107 193 100 714.6 0 100 194 140 714.6 0 140 195 140 714.6 0 140 196 100 714.6 0 100 197 89 714.6 0 89 198 105 714.6 0 105 199 176 714.6 0 176 200 149 714.6 0 149 201 69 714.6 0 69 202 48 714.6 0 48 203 59 714.6 0 59 204 92 714.6 0 92 205 117 714.6 0 117 206 141 714.6 0 141 207 104 714.6 0 104 208 118 714.6 0 118 209 80 714.6 0 80 210 103 714.6 0 103 211 87 714.6 0 87 212 130 714.6 0 130 213 129 714.6 0 129 214 177 714.6 0 177 215 193 714.6 0 193 216 153 714.6 0 153 217 112 714.6 0 112 218 124 714.6 0 124 219 141 714.6 0 141 220 138 714.6 0 138 221 105 714.6 0 105 222 104 714.6 0 104 223 83 714.6 0 83 224 90 714.6 0 90 225 148 714.6 0 148 226 91 714.6 0 91 227 145 714.6 0 145 228 157 714.6 0 157 229 135 714.6 0 135 230 104 714.6 0 104 231 75 714.6 0 75 232 78 714.6 0 78 233 91 714.6 0 91 234 70 714.6 0 70 235 66 714.6 0 66 236 104 714.6 0 104 237 143 714.6 0 143 238 138 714.6 0 138 239 142 714.6 0 142 240 158 714.6 0 158 241 116 714.6 0 116 242 72 714.6 0 72 243 82 714.6 0 82 244 83 714.6 0 83 245 85 714.6 0 85 246 71 714.6 0 71 247 85 714.6 0 85 248 166 714.6 0 166 249 140 714.6 0 140 250 73 714.6 0 73 251 38 714.6 0 38 252 33 714.6 0 33 253 49 714.6 0 49 254 75 714.6 0 75 255 95 714.6 0 95 256 99 714.6 0 99 257 124 714.6 0 124 258 122 714.6 0 122 259 112 714.6 0 112 260 178 714.6 0 178 261 94 714.6 0 94 262 132 714.6 0 132 263 168 714.6 0 168 264 141 714.6 0 141 265 119 714.6 0 119 266 145 714.6 0 145 267 134 714.6 0 134 268 185 714.6 0 185 269 166 714.6 0 166 270 113 714.6 0 113 271 109 714.6 0 109 272 141 714.6 0 141 273 1702 714.6 0 1702 274 1439 714.6 0 1439 275 2727 714.6 0 2727 276 219 714.6 0 219 277 166 714.6 0 166 278 135 714.6 0 135 279 178 714.6 0 178 280 480 714.6 0 480 281 212 714.6 0 212 282 189 714.6 0 189 283 365 714.6 0 365 284 290 714.6 0 290 285 338 714.6 0 338 286 319 714.6 0 319 287 655 714.6 0 655 288 396 714.6 0 396 289 252 714.6 0 252 290 209 714.6 0 209 291 202 714.6 0 202 292 316 714.6 0 316 293 5174 714.6 0 5174 294 2595 714.6 0 2595 295 10629 714.6 0 10629 296 403 714.6 0 403 297 653 714.6 0 653 298 1957 714.6 0 1957 299 2380 714.6 0 2380 300 2567 714.6 0 2567 301 606 714.6 0 606 Finished in 2.70 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 56,488 Reads with adapters: 29,016 (51.4%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 29,012 (51.4%) Total basepairs processed: 14,916,412 bp Total written (filtered): 8,007,670 bp (53.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 29016 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 8 0.0 3 0 0 8 16 207 0.0 3 0 202 5 17 28682 0.0 3 28258 413 11 18 78 0.0 3 7 67 3 1 19 13 0.0 3 2 0 10 1 20 17 0.0 3 0 0 0 17 22 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 1 65 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.01 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 29,012 Reads with adapters: 469 (1.6%) Reads written (passing filters): 29,012 (100.0%) Total basepairs processed: 8,007,670 bp Total written (filtered): 8,006,260 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 469 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.2% C: 30.1% G: 22.0% T: 6.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 466 453.3 0 466 4 3 113.3 0 3