This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.44 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 236,463 (8.1%) Reads that were too short: 122,943 (4.2%) Reads written (passing filters): 113,520 (3.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 23,100,520 bp (2.6%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 236463 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 854 714.6 0 854 7 644 714.6 0 644 8 35422 714.6 0 35422 9 4641 714.6 0 4641 10 67 714.6 0 67 11 40 714.6 0 40 12 38 714.6 0 38 13 36 714.6 0 36 14 40 714.6 0 40 15 29 714.6 0 29 16 30 714.6 0 30 17 41 714.6 0 41 18 45 714.6 0 45 19 38 714.6 0 38 20 33 714.6 0 33 21 39 714.6 0 39 22 23 714.6 0 23 23 30 714.6 0 30 24 22 714.6 0 22 25 26 714.6 0 26 26 25 714.6 0 25 27 18 714.6 0 18 28 40 714.6 0 40 29 28 714.6 0 28 30 45 714.6 0 45 31 25 714.6 0 25 32 38 714.6 0 38 33 31 714.6 0 31 34 19 714.6 0 19 35 25 714.6 0 25 36 28 714.6 0 28 37 51 714.6 0 51 38 92 714.6 0 92 39 63 714.6 0 63 40 28 714.6 0 28 41 44 714.6 0 44 42 37 714.6 0 37 43 35 714.6 0 35 44 281 714.6 0 281 45 49 714.6 0 49 46 31 714.6 0 31 47 35 714.6 0 35 48 56 714.6 0 56 49 43 714.6 0 43 50 42 714.6 0 42 51 87 714.6 0 87 52 31 714.6 0 31 53 41 714.6 0 41 54 136 714.6 0 136 55 37 714.6 0 37 56 117 714.6 0 117 57 28 714.6 0 28 58 46 714.6 0 46 59 31 714.6 0 31 60 24 714.6 0 24 61 26 714.6 0 26 62 44 714.6 0 44 63 25 714.6 0 25 64 42 714.6 0 42 65 34 714.6 0 34 66 36 714.6 0 36 67 30 714.6 0 30 68 43 714.6 0 43 69 21 714.6 0 21 70 17 714.6 0 17 71 111 714.6 0 111 72 59 714.6 0 59 73 770 714.6 0 770 74 408 714.6 0 408 75 442 714.6 0 442 76 73 714.6 0 73 77 165 714.6 0 165 78 185 714.6 0 185 79 140 714.6 0 140 80 70 714.6 0 70 81 164 714.6 0 164 82 228 714.6 0 228 83 196 714.6 0 196 84 209 714.6 0 209 85 339 714.6 0 339 86 318 714.6 0 318 87 198 714.6 0 198 88 208 714.6 0 208 89 113 714.6 0 113 90 197 714.6 0 197 91 261 714.6 0 261 92 200 714.6 0 200 93 330 714.6 0 330 94 566 714.6 0 566 95 378 714.6 0 378 96 327 714.6 0 327 97 373 714.6 0 373 98 386 714.6 0 386 99 373 714.6 0 373 100 111 714.6 0 111 101 77 714.6 0 77 102 97 714.6 0 97 103 125 714.6 0 125 104 74 714.6 0 74 105 59 714.6 0 59 106 107 714.6 0 107 107 198 714.6 0 198 108 206 714.6 0 206 109 188 714.6 0 188 110 187 714.6 0 187 111 163 714.6 0 163 112 198 714.6 0 198 113 217 714.6 0 217 114 294 714.6 0 294 115 470 714.6 0 470 116 339 714.6 0 339 117 261 714.6 0 261 118 237 714.6 0 237 119 389 714.6 0 389 120 461 714.6 0 461 121 391 714.6 0 391 122 279 714.6 0 279 123 268 714.6 0 268 124 345 714.6 0 345 125 493 714.6 0 493 126 505 714.6 0 505 127 628 714.6 0 628 128 496 714.6 0 496 129 501 714.6 0 501 130 423 714.6 0 423 131 335 714.6 0 335 132 253 714.6 0 253 133 375 714.6 0 375 134 783 714.6 0 783 135 855 714.6 0 855 136 751 714.6 0 751 137 710 714.6 0 710 138 408 714.6 0 408 139 342 714.6 0 342 140 366 714.6 0 366 141 491 714.6 0 491 142 340 714.6 0 340 143 347 714.6 0 347 144 339 714.6 0 339 145 1032 714.6 0 1032 146 1046 714.6 0 1046 147 952 714.6 0 952 148 525 714.6 0 525 149 426 714.6 0 426 150 391 714.6 0 391 151 500 714.6 0 500 152 513 714.6 0 513 153 670 714.6 0 670 154 781 714.6 0 781 155 764 714.6 0 764 156 695 714.6 0 695 157 756 714.6 0 756 158 674 714.6 0 674 159 563 714.6 0 563 160 576 714.6 0 576 161 639 714.6 0 639 162 517 714.6 0 517 163 447 714.6 0 447 164 615 714.6 0 615 165 1182 714.6 0 1182 166 1096 714.6 0 1096 167 659 714.6 0 659 168 460 714.6 0 460 169 449 714.6 0 449 170 425 714.6 0 425 171 458 714.6 0 458 172 498 714.6 0 498 173 2044 714.6 0 2044 174 2932 714.6 0 2932 175 1623 714.6 0 1623 176 856 714.6 0 856 177 470 714.6 0 470 178 451 714.6 0 451 179 636 714.6 0 636 180 761 714.6 0 761 181 2690 714.6 0 2690 182 1139 714.6 0 1139 183 1159 714.6 0 1159 184 974 714.6 0 974 185 794 714.6 0 794 186 1238 714.6 0 1238 187 797 714.6 0 797 188 473 714.6 0 473 189 473 714.6 0 473 190 646 714.6 0 646 191 791 714.6 0 791 192 1077 714.6 0 1077 193 1397 714.6 0 1397 194 677 714.6 0 677 195 329 714.6 0 329 196 374 714.6 0 374 197 508 714.6 0 508 198 811 714.6 0 811 199 613 714.6 0 613 200 464 714.6 0 464 201 502 714.6 0 502 202 684 714.6 0 684 203 728 714.6 0 728 204 866 714.6 0 866 205 639 714.6 0 639 206 703 714.6 0 703 207 781 714.6 0 781 208 592 714.6 0 592 209 545 714.6 0 545 210 512 714.6 0 512 211 646 714.6 0 646 212 777 714.6 0 777 213 690 714.6 0 690 214 823 714.6 0 823 215 791 714.6 0 791 216 705 714.6 0 705 217 822 714.6 0 822 218 891 714.6 0 891 219 1190 714.6 0 1190 220 932 714.6 0 932 221 1009 714.6 0 1009 222 796 714.6 0 796 223 887 714.6 0 887 224 732 714.6 0 732 225 3491 714.6 0 3491 226 4637 714.6 0 4637 227 7042 714.6 0 7042 228 773 714.6 0 773 229 623 714.6 0 623 230 1288 714.6 0 1288 231 2163 714.6 0 2163 232 2460 714.6 0 2460 233 1239 714.6 0 1239 234 2146 714.6 0 2146 235 981 714.6 0 981 236 601 714.6 0 601 237 666 714.6 0 666 238 779 714.6 0 779 239 761 714.6 0 761 240 477 714.6 0 477 241 759 714.6 0 759 242 874 714.6 0 874 243 733 714.6 0 733 244 698 714.6 0 698 245 661 714.6 0 661 246 1335 714.6 0 1335 247 1647 714.6 0 1647 248 1252 714.6 0 1252 249 285 714.6 0 285 250 1113 714.6 0 1113 251 2392 714.6 0 2392 252 3860 714.6 0 3860 253 1959 714.6 0 1959 254 1512 714.6 0 1512 255 1130 714.6 0 1130 256 1081 714.6 0 1081 257 789 714.6 0 789 258 558 714.6 0 558 259 283 714.6 0 283 260 351 714.6 0 351 261 399 714.6 0 399 262 423 714.6 0 423 263 520 714.6 0 520 264 523 714.6 0 523 265 686 714.6 0 686 266 1100 714.6 0 1100 267 965 714.6 0 965 268 667 714.6 0 667 269 471 714.6 0 471 270 640 714.6 0 640 271 840 714.6 0 840 272 938 714.6 0 938 273 812 714.6 0 812 274 656 714.6 0 656 275 454 714.6 0 454 276 459 714.6 0 459 277 738 714.6 0 738 278 1070 714.6 0 1070 279 1376 714.6 0 1376 280 1928 714.6 0 1928 281 1604 714.6 0 1604 282 1076 714.6 0 1076 283 673 714.6 0 673 284 773 714.6 0 773 285 1244 714.6 0 1244 286 995 714.6 0 995 287 620 714.6 0 620 288 524 714.6 0 524 289 549 714.6 0 549 290 845 714.6 0 845 291 1147 714.6 0 1147 292 1167 714.6 0 1167 293 657 714.6 0 657 294 689 714.6 0 689 295 837 714.6 0 837 296 811 714.6 0 811 297 823 714.6 0 823 298 1198 714.6 0 1198 299 1740 714.6 0 1740 300 2265 714.6 0 2265 301 15901 714.6 0 15901 Finished in 4.88 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 113,520 Reads with adapters: 4,692 (4.1%) Reads that were too short: 43 (0.0%) Reads written (passing filters): 4,649 (4.1%) Total basepairs processed: 23,100,520 bp Total written (filtered): 1,263,175 bp (5.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4692 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 2 0 1 19 48 0.0 3 0 43 2 1 2 20 4557 0.0 4 4281 249 18 5 4 21 14 0.0 4 1 13 22 9 0.0 4 3 3 3 23 5 0.0 4 1 1 0 2 1 25 1 0.0 4 0 0 0 0 1 26 3 0.0 4 0 1 0 0 2 30 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 35 2 0.0 4 0 0 0 0 2 37 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 99 3 0.0 4 0 0 0 0 3 102 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 239 2 0.0 4 0 0 0 0 2 240 1 0.0 4 0 0 0 0 1 241 2 0.0 4 0 0 0 0 2 262 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 4,649 Reads with adapters: 326 (7.0%) Reads written (passing filters): 4,649 (100.0%) Total basepairs processed: 1,263,175 bp Total written (filtered): 1,259,128 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 326 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.3% C: 5.2% G: 84.7% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 72.6 0 9 6 15 1.1 0 15 9 29 0.0 0 2 27 10 129 0.0 1 5 124 11 140 0.0 1 0 140 153 1 0.0 1 1 208 2 0.0 1 2 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.