This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.54 s (26 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 68,798 (2.4%) Reads that were too short: 32,744 (1.1%) Reads written (passing filters): 36,054 (1.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 7,153,557 bp (0.8%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 68798 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 111 714.6 0 111 7 2009 714.6 0 2009 8 6342 714.6 0 6342 9 41 714.6 0 41 10 30 714.6 0 30 11 33 714.6 0 33 12 27 714.6 0 27 13 30 714.6 0 30 14 35 714.6 0 35 15 26 714.6 0 26 16 26 714.6 0 26 17 29 714.6 0 29 18 33 714.6 0 33 19 26 714.6 0 26 20 74 714.6 0 74 21 67 714.6 0 67 22 63 714.6 0 63 23 369 714.6 0 369 24 23 714.6 0 23 25 35 714.6 0 35 26 36 714.6 0 36 27 31 714.6 0 31 28 31 714.6 0 31 29 24 714.6 0 24 30 32 714.6 0 32 31 253 714.6 0 253 32 60 714.6 0 60 33 58 714.6 0 58 34 73 714.6 0 73 35 53 714.6 0 53 36 55 714.6 0 55 37 34 714.6 0 34 38 37 714.6 0 37 39 74 714.6 0 74 40 64 714.6 0 64 41 90 714.6 0 90 42 28 714.6 0 28 43 87 714.6 0 87 44 28 714.6 0 28 45 25 714.6 0 25 46 34 714.6 0 34 47 29 714.6 0 29 48 27 714.6 0 27 49 22 714.6 0 22 50 21 714.6 0 21 51 31 714.6 0 31 52 59 714.6 0 59 53 46 714.6 0 46 54 37 714.6 0 37 55 34 714.6 0 34 56 29 714.6 0 29 57 75 714.6 0 75 58 37 714.6 0 37 59 29 714.6 0 29 60 54 714.6 0 54 61 43 714.6 0 43 62 26 714.6 0 26 63 23 714.6 0 23 64 37 714.6 0 37 65 49 714.6 0 49 66 27 714.6 0 27 67 28 714.6 0 28 68 32 714.6 0 32 69 24 714.6 0 24 70 50 714.6 0 50 71 36 714.6 0 36 72 30 714.6 0 30 73 40 714.6 0 40 74 53 714.6 0 53 75 45 714.6 0 45 76 56 714.6 0 56 77 58 714.6 0 58 78 95 714.6 0 95 79 80 714.6 0 80 80 112 714.6 0 112 81 123 714.6 0 123 82 154 714.6 0 154 83 114 714.6 0 114 84 105 714.6 0 105 85 230 714.6 0 230 86 341 714.6 0 341 87 130 714.6 0 130 88 67 714.6 0 67 89 94 714.6 0 94 90 66 714.6 0 66 91 157 714.6 0 157 92 321 714.6 0 321 93 174 714.6 0 174 94 144 714.6 0 144 95 166 714.6 0 166 96 135 714.6 0 135 97 149 714.6 0 149 98 181 714.6 0 181 99 229 714.6 0 229 100 314 714.6 0 314 101 222 714.6 0 222 102 240 714.6 0 240 103 334 714.6 0 334 104 203 714.6 0 203 105 58 714.6 0 58 106 37 714.6 0 37 107 36 714.6 0 36 108 27 714.6 0 27 109 72 714.6 0 72 110 118 714.6 0 118 111 197 714.6 0 197 112 142 714.6 0 142 113 161 714.6 0 161 114 111 714.6 0 111 115 136 714.6 0 136 116 219 714.6 0 219 117 208 714.6 0 208 118 221 714.6 0 221 119 164 714.6 0 164 120 103 714.6 0 103 121 81 714.6 0 81 122 100 714.6 0 100 123 255 714.6 0 255 124 351 714.6 0 351 125 271 714.6 0 271 126 138 714.6 0 138 127 113 714.6 0 113 128 119 714.6 0 119 129 120 714.6 0 120 130 147 714.6 0 147 131 204 714.6 0 204 132 284 714.6 0 284 133 337 714.6 0 337 134 159 714.6 0 159 135 166 714.6 0 166 136 152 714.6 0 152 137 242 714.6 0 242 138 356 714.6 0 356 139 287 714.6 0 287 140 175 714.6 0 175 141 141 714.6 0 141 142 321 714.6 0 321 143 501 714.6 0 501 144 543 714.6 0 543 145 433 714.6 0 433 146 156 714.6 0 156 147 121 714.6 0 121 148 125 714.6 0 125 149 160 714.6 0 160 150 183 714.6 0 183 151 157 714.6 0 157 152 185 714.6 0 185 153 148 714.6 0 148 154 150 714.6 0 150 155 114 714.6 0 114 156 145 714.6 0 145 157 163 714.6 0 163 158 265 714.6 0 265 159 237 714.6 0 237 160 160 714.6 0 160 161 147 714.6 0 147 162 255 714.6 0 255 163 178 714.6 0 178 164 173 714.6 0 173 165 138 714.6 0 138 166 152 714.6 0 152 167 216 714.6 0 216 168 234 714.6 0 234 169 276 714.6 0 276 170 372 714.6 0 372 171 399 714.6 0 399 172 297 714.6 0 297 173 140 714.6 0 140 174 117 714.6 0 117 175 86 714.6 0 86 176 97 714.6 0 97 177 282 714.6 0 282 178 348 714.6 0 348 179 323 714.6 0 323 180 205 714.6 0 205 181 152 714.6 0 152 182 133 714.6 0 133 183 231 714.6 0 231 184 331 714.6 0 331 185 256 714.6 0 256 186 193 714.6 0 193 187 375 714.6 0 375 188 319 714.6 0 319 189 323 714.6 0 323 190 161 714.6 0 161 191 140 714.6 0 140 192 168 714.6 0 168 193 201 714.6 0 201 194 272 714.6 0 272 195 160 714.6 0 160 196 152 714.6 0 152 197 183 714.6 0 183 198 351 714.6 0 351 199 326 714.6 0 326 200 140 714.6 0 140 201 74 714.6 0 74 202 94 714.6 0 94 203 100 714.6 0 100 204 200 714.6 0 200 205 219 714.6 0 219 206 225 714.6 0 225 207 196 714.6 0 196 208 151 714.6 0 151 209 139 714.6 0 139 210 274 714.6 0 274 211 480 714.6 0 480 212 335 714.6 0 335 213 277 714.6 0 277 214 695 714.6 0 695 215 852 714.6 0 852 216 639 714.6 0 639 217 211 714.6 0 211 218 205 714.6 0 205 219 306 714.6 0 306 220 182 714.6 0 182 221 113 714.6 0 113 222 136 714.6 0 136 223 1162 714.6 0 1162 224 692 714.6 0 692 225 521 714.6 0 521 226 214 714.6 0 214 227 197 714.6 0 197 228 241 714.6 0 241 229 222 714.6 0 222 230 146 714.6 0 146 231 111 714.6 0 111 232 87 714.6 0 87 233 100 714.6 0 100 234 115 714.6 0 115 235 190 714.6 0 190 236 237 714.6 0 237 237 190 714.6 0 190 238 300 714.6 0 300 239 192 714.6 0 192 240 309 714.6 0 309 241 163 714.6 0 163 242 92 714.6 0 92 243 120 714.6 0 120 244 112 714.6 0 112 245 237 714.6 0 237 246 469 714.6 0 469 247 510 714.6 0 510 248 258 714.6 0 258 249 84 714.6 0 84 250 52 714.6 0 52 251 67 714.6 0 67 252 78 714.6 0 78 253 128 714.6 0 128 254 157 714.6 0 157 255 183 714.6 0 183 256 293 714.6 0 293 257 443 714.6 0 443 258 353 714.6 0 353 259 248 714.6 0 248 260 140 714.6 0 140 261 72 714.6 0 72 262 101 714.6 0 101 263 222 714.6 0 222 264 363 714.6 0 363 265 530 714.6 0 530 266 254 714.6 0 254 267 330 714.6 0 330 268 255 714.6 0 255 269 164 714.6 0 164 270 136 714.6 0 136 271 250 714.6 0 250 272 1491 714.6 0 1491 273 3694 714.6 0 3694 274 2659 714.6 0 2659 275 154 714.6 0 154 276 106 714.6 0 106 277 122 714.6 0 122 278 218 714.6 0 218 279 256 714.6 0 256 280 212 714.6 0 212 281 161 714.6 0 161 282 58 714.6 0 58 283 132 714.6 0 132 284 213 714.6 0 213 285 569 714.6 0 569 286 589 714.6 0 589 287 384 714.6 0 384 288 132 714.6 0 132 289 80 714.6 0 80 290 52 714.6 0 52 291 89 714.6 0 89 292 136 714.6 0 136 293 249 714.6 0 249 294 193 714.6 0 193 295 150 714.6 0 150 296 128 714.6 0 128 297 120 714.6 0 120 298 212 714.6 0 212 299 599 714.6 0 599 300 834 714.6 0 834 301 633 714.6 0 633 Finished in 1.51 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 36,054 Reads with adapters: 6,483 (18.0%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 6,468 (17.9%) Total basepairs processed: 7,153,557 bp Total written (filtered): 1,765,266 bp (24.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6483 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 68 0.0 3 0 62 2 4 20 6357 0.0 4 6184 136 25 6 6 21 21 0.0 4 4 12 0 3 2 22 7 0.0 4 1 0 5 0 1 23 5 0.0 4 1 0 0 3 1 28 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 0 2 81 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 111 3 0.0 4 0 0 0 2 1 123 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 6,468 Reads with adapters: 545 (8.4%) Reads written (passing filters): 6,468 (100.0%) Total basepairs processed: 1,765,266 bp Total written (filtered): 1,750,205 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 545 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.5% C: 7.5% G: 83.1% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 101.1 0 2 10 53 0.0 1 0 53 11 449 0.0 1 3 446 12 2 0.0 1 0 2 151 1 0.0 1 1 224 13 0.0 1 12 1 259 1 0.0 1 1 260 24 0.0 1 23 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.