This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.46 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 203,291 (6.9%) Reads that were too short: 98,775 (3.4%) Reads written (passing filters): 104,516 (3.6%) Total basepairs processed: 881,011,047 bp Total written (filtered): 21,239,276 bp (2.4%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 203291 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 791 714.6 0 791 7 23022 714.6 0 23022 8 160 714.6 0 160 9 6148 714.6 0 6148 10 44 714.6 0 44 11 26 714.6 0 26 12 31 714.6 0 31 13 18 714.6 0 18 14 31 714.6 0 31 15 38 714.6 0 38 16 29 714.6 0 29 17 18 714.6 0 18 18 32 714.6 0 32 19 21 714.6 0 21 20 21 714.6 0 21 21 13 714.6 0 13 22 29 714.6 0 29 23 27 714.6 0 27 24 27 714.6 0 27 25 31 714.6 0 31 26 65 714.6 0 65 27 3656 714.6 0 3656 28 376 714.6 0 376 29 20 714.6 0 20 30 35 714.6 0 35 31 26 714.6 0 26 32 32 714.6 0 32 33 35 714.6 0 35 34 30 714.6 0 30 35 17 714.6 0 17 36 30 714.6 0 30 37 51 714.6 0 51 38 29 714.6 0 29 39 87 714.6 0 87 40 23 714.6 0 23 41 30 714.6 0 30 42 37 714.6 0 37 43 25 714.6 0 25 44 31 714.6 0 31 45 27 714.6 0 27 46 21 714.6 0 21 47 26 714.6 0 26 48 19 714.6 0 19 49 13 714.6 0 13 50 20 714.6 0 20 51 31 714.6 0 31 52 23 714.6 0 23 53 27 714.6 0 27 54 20 714.6 0 20 55 32 714.6 0 32 56 66 714.6 0 66 57 651 714.6 0 651 58 404 714.6 0 404 59 44 714.6 0 44 60 29 714.6 0 29 61 22 714.6 0 22 62 13 714.6 0 13 63 45 714.6 0 45 64 13 714.6 0 13 65 16 714.6 0 16 66 29 714.6 0 29 67 16 714.6 0 16 68 49 714.6 0 49 69 29 714.6 0 29 70 21 714.6 0 21 71 21 714.6 0 21 72 26 714.6 0 26 73 17 714.6 0 17 74 19 714.6 0 19 75 18 714.6 0 18 76 25 714.6 0 25 77 54 714.6 0 54 78 39 714.6 0 39 79 95 714.6 0 95 80 147 714.6 0 147 81 116 714.6 0 116 82 232 714.6 0 232 83 254 714.6 0 254 84 624 714.6 0 624 85 609 714.6 0 609 86 282 714.6 0 282 87 157 714.6 0 157 88 835 714.6 0 835 89 594 714.6 0 594 90 1234 714.6 0 1234 91 639 714.6 0 639 92 957 714.6 0 957 93 258 714.6 0 258 94 334 714.6 0 334 95 427 714.6 0 427 96 329 714.6 0 329 97 149 714.6 0 149 98 170 714.6 0 170 99 221 714.6 0 221 100 433 714.6 0 433 101 649 714.6 0 649 102 415 714.6 0 415 103 338 714.6 0 338 104 449 714.6 0 449 105 315 714.6 0 315 106 175 714.6 0 175 107 138 714.6 0 138 108 305 714.6 0 305 109 457 714.6 0 457 110 239 714.6 0 239 111 215 714.6 0 215 112 246 714.6 0 246 113 253 714.6 0 253 114 197 714.6 0 197 115 593 714.6 0 593 116 515 714.6 0 515 117 349 714.6 0 349 118 160 714.6 0 160 119 171 714.6 0 171 120 199 714.6 0 199 121 489 714.6 0 489 122 1139 714.6 0 1139 123 727 714.6 0 727 124 408 714.6 0 408 125 293 714.6 0 293 126 327 714.6 0 327 127 412 714.6 0 412 128 285 714.6 0 285 129 264 714.6 0 264 130 576 714.6 0 576 131 639 714.6 0 639 132 443 714.6 0 443 133 300 714.6 0 300 134 356 714.6 0 356 135 428 714.6 0 428 136 530 714.6 0 530 137 475 714.6 0 475 138 383 714.6 0 383 139 343 714.6 0 343 140 541 714.6 0 541 141 2321 714.6 0 2321 142 2079 714.6 0 2079 143 1075 714.6 0 1075 144 380 714.6 0 380 145 320 714.6 0 320 146 547 714.6 0 547 147 849 714.6 0 849 148 1641 714.6 0 1641 149 693 714.6 0 693 150 487 714.6 0 487 151 1125 714.6 0 1125 152 1726 714.6 0 1726 153 1030 714.6 0 1030 154 844 714.6 0 844 155 774 714.6 0 774 156 742 714.6 0 742 157 1803 714.6 0 1803 158 1300 714.6 0 1300 159 892 714.6 0 892 160 647 714.6 0 647 161 707 714.6 0 707 162 1028 714.6 0 1028 163 759 714.6 0 759 164 412 714.6 0 412 165 335 714.6 0 335 166 602 714.6 0 602 167 678 714.6 0 678 168 494 714.6 0 494 169 604 714.6 0 604 170 542 714.6 0 542 171 404 714.6 0 404 172 267 714.6 0 267 173 188 714.6 0 188 174 330 714.6 0 330 175 688 714.6 0 688 176 585 714.6 0 585 177 386 714.6 0 386 178 345 714.6 0 345 179 288 714.6 0 288 180 333 714.6 0 333 181 389 714.6 0 389 182 1073 714.6 0 1073 183 1675 714.6 0 1675 184 846 714.6 0 846 185 545 714.6 0 545 186 501 714.6 0 501 187 823 714.6 0 823 188 730 714.6 0 730 189 465 714.6 0 465 190 317 714.6 0 317 191 315 714.6 0 315 192 378 714.6 0 378 193 352 714.6 0 352 194 291 714.6 0 291 195 397 714.6 0 397 196 560 714.6 0 560 197 466 714.6 0 466 198 448 714.6 0 448 199 282 714.6 0 282 200 191 714.6 0 191 201 273 714.6 0 273 202 447 714.6 0 447 203 647 714.6 0 647 204 500 714.6 0 500 205 514 714.6 0 514 206 578 714.6 0 578 207 501 714.6 0 501 208 781 714.6 0 781 209 1575 714.6 0 1575 210 1422 714.6 0 1422 211 1003 714.6 0 1003 212 1336 714.6 0 1336 213 14102 714.6 0 14102 214 10347 714.6 0 10347 215 4943 714.6 0 4943 216 836 714.6 0 836 217 548 714.6 0 548 218 509 714.6 0 509 219 381 714.6 0 381 220 501 714.6 0 501 221 2133 714.6 0 2133 222 2814 714.6 0 2814 223 2292 714.6 0 2292 224 653 714.6 0 653 225 445 714.6 0 445 226 671 714.6 0 671 227 654 714.6 0 654 228 505 714.6 0 505 229 424 714.6 0 424 230 316 714.6 0 316 231 577 714.6 0 577 232 489 714.6 0 489 233 551 714.6 0 551 234 717 714.6 0 717 235 830 714.6 0 830 236 583 714.6 0 583 237 1079 714.6 0 1079 238 469 714.6 0 469 239 371 714.6 0 371 240 400 714.6 0 400 241 347 714.6 0 347 242 550 714.6 0 550 243 816 714.6 0 816 244 1437 714.6 0 1437 245 995 714.6 0 995 246 579 714.6 0 579 247 486 714.6 0 486 248 356 714.6 0 356 249 133 714.6 0 133 250 97 714.6 0 97 251 315 714.6 0 315 252 663 714.6 0 663 253 784 714.6 0 784 254 813 714.6 0 813 255 842 714.6 0 842 256 637 714.6 0 637 257 1976 714.6 0 1976 258 2002 714.6 0 2002 259 913 714.6 0 913 260 392 714.6 0 392 261 483 714.6 0 483 262 678 714.6 0 678 263 719 714.6 0 719 264 817 714.6 0 817 265 815 714.6 0 815 266 593 714.6 0 593 267 689 714.6 0 689 268 391 714.6 0 391 269 232 714.6 0 232 270 442 714.6 0 442 271 896 714.6 0 896 272 915 714.6 0 915 273 848 714.6 0 848 274 573 714.6 0 573 275 510 714.6 0 510 276 519 714.6 0 519 277 670 714.6 0 670 278 601 714.6 0 601 279 952 714.6 0 952 280 492 714.6 0 492 281 666 714.6 0 666 282 718 714.6 0 718 283 704 714.6 0 704 284 1313 714.6 0 1313 285 1102 714.6 0 1102 286 1056 714.6 0 1056 287 541 714.6 0 541 288 205 714.6 0 205 289 177 714.6 0 177 290 272 714.6 0 272 291 831 714.6 0 831 292 1105 714.6 0 1105 293 563 714.6 0 563 294 560 714.6 0 560 295 377 714.6 0 377 296 160 714.6 0 160 297 218 714.6 0 218 298 323 714.6 0 323 299 446 714.6 0 446 300 447 714.6 0 447 301 579 714.6 0 579 Finished in 3.93 s (38 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 104,516 Reads with adapters: 6,321 (6.0%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 6,296 (6.0%) Total basepairs processed: 21,239,276 bp Total written (filtered): 1,729,591 bp (8.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6321 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 1 16 57 0.0 3 0 53 3 1 17 6190 0.0 3 6045 141 4 18 16 0.0 3 1 12 3 19 9 0.0 3 0 0 5 4 20 5 0.0 3 0 0 1 4 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 45 4 0.0 3 0 0 0 4 46 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 192 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 6,296 Reads with adapters: 593 (9.4%) Reads written (passing filters): 6,296 (100.0%) Total basepairs processed: 1,729,591 bp Total written (filtered): 1,727,811 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 593 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.0% C: 12.6% G: 71.8% T: 0.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 592 98.4 0 592 4 1 24.6 0 1