This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 79.07 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 269,832 (9.2%) Reads that were too short: 14,632 (0.5%) Reads written (passing filters): 255,200 (8.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 58,945,732 bp (6.7%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 269832 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 705 714.6 0 705 7 1950 714.6 0 1950 8 58632 714.6 0 58632 9 4438 714.6 0 4438 10 48 714.6 0 48 11 45 714.6 0 45 12 41 714.6 0 41 13 65 714.6 0 65 14 50 714.6 0 50 15 38 714.6 0 38 16 48 714.6 0 48 17 48 714.6 0 48 18 46 714.6 0 46 19 45 714.6 0 45 20 42 714.6 0 42 21 41 714.6 0 41 22 49 714.6 0 49 23 74 714.6 0 74 24 69 714.6 0 69 25 43 714.6 0 43 26 49 714.6 0 49 27 40 714.6 0 40 28 49 714.6 0 49 29 1161 714.6 0 1161 30 278 714.6 0 278 31 53 714.6 0 53 32 62 714.6 0 62 33 51 714.6 0 51 34 79 714.6 0 79 35 64 714.6 0 64 36 76 714.6 0 76 37 82 714.6 0 82 38 134 714.6 0 134 39 75 714.6 0 75 40 67 714.6 0 67 41 69 714.6 0 69 42 106 714.6 0 106 43 93 714.6 0 93 44 90 714.6 0 90 45 140 714.6 0 140 46 181 714.6 0 181 47 291 714.6 0 291 48 623 714.6 0 623 49 1439 714.6 0 1439 50 306 714.6 0 306 51 517 714.6 0 517 52 1236 714.6 0 1236 53 615 714.6 0 615 54 474 714.6 0 474 55 710 714.6 0 710 56 714 714.6 0 714 57 668 714.6 0 668 58 674 714.6 0 674 59 986 714.6 0 986 60 899 714.6 0 899 61 1706 714.6 0 1706 62 35670 714.6 0 35670 63 29442 714.6 0 29442 64 48545 714.6 0 48545 65 420 714.6 0 420 66 36 714.6 0 36 67 34 714.6 0 34 68 43 714.6 0 43 69 38 714.6 0 38 70 61 714.6 0 61 71 83 714.6 0 83 72 146 714.6 0 146 73 141 714.6 0 141 74 50 714.6 0 50 75 89 714.6 0 89 76 992 714.6 0 992 77 1146 714.6 0 1146 78 240 714.6 0 240 79 51 714.6 0 51 80 41 714.6 0 41 81 36 714.6 0 36 82 57 714.6 0 57 83 73 714.6 0 73 84 33 714.6 0 33 85 63 714.6 0 63 86 47 714.6 0 47 87 50 714.6 0 50 88 33 714.6 0 33 89 23 714.6 0 23 90 28 714.6 0 28 91 31 714.6 0 31 92 44 714.6 0 44 93 89 714.6 0 89 94 76 714.6 0 76 95 51 714.6 0 51 96 40 714.6 0 40 97 46 714.6 0 46 98 49 714.6 0 49 99 41 714.6 0 41 100 51 714.6 0 51 101 61 714.6 0 61 102 68 714.6 0 68 103 55 714.6 0 55 104 63 714.6 0 63 105 201 714.6 0 201 106 100 714.6 0 100 107 79 714.6 0 79 108 99 714.6 0 99 109 36 714.6 0 36 110 57 714.6 0 57 111 53 714.6 0 53 112 58 714.6 0 58 113 46 714.6 0 46 114 50 714.6 0 50 115 65 714.6 0 65 116 63 714.6 0 63 117 47 714.6 0 47 118 63 714.6 0 63 119 54 714.6 0 54 120 44 714.6 0 44 121 52 714.6 0 52 122 48 714.6 0 48 123 61 714.6 0 61 124 76 714.6 0 76 125 59 714.6 0 59 126 91 714.6 0 91 127 109 714.6 0 109 128 102 714.6 0 102 129 78 714.6 0 78 130 58 714.6 0 58 131 61 714.6 0 61 132 67 714.6 0 67 133 95 714.6 0 95 134 104 714.6 0 104 135 85 714.6 0 85 136 75 714.6 0 75 137 81 714.6 0 81 138 71 714.6 0 71 139 70 714.6 0 70 140 85 714.6 0 85 141 111 714.6 0 111 142 134 714.6 0 134 143 221 714.6 0 221 144 1771 714.6 0 1771 145 1536 714.6 0 1536 146 1257 714.6 0 1257 147 153 714.6 0 153 148 79 714.6 0 79 149 149 714.6 0 149 150 203 714.6 0 203 151 173 714.6 0 173 152 98 714.6 0 98 153 85 714.6 0 85 154 118 714.6 0 118 155 185 714.6 0 185 156 1098 714.6 0 1098 157 1183 714.6 0 1183 158 443 714.6 0 443 159 194 714.6 0 194 160 184 714.6 0 184 161 300 714.6 0 300 162 439 714.6 0 439 163 1099 714.6 0 1099 164 23383 714.6 0 23383 165 5534 714.6 0 5534 166 2494 714.6 0 2494 167 229 714.6 0 229 168 239 714.6 0 239 169 635 714.6 0 635 170 725 714.6 0 725 171 810 714.6 0 810 172 403 714.6 0 403 173 335 714.6 0 335 174 194 714.6 0 194 175 84 714.6 0 84 176 97 714.6 0 97 177 112 714.6 0 112 178 97 714.6 0 97 179 271 714.6 0 271 180 192 714.6 0 192 181 171 714.6 0 171 182 155 714.6 0 155 183 115 714.6 0 115 184 214 714.6 0 214 185 224 714.6 0 224 186 295 714.6 0 295 187 118 714.6 0 118 188 130 714.6 0 130 189 748 714.6 0 748 190 1161 714.6 0 1161 191 1190 714.6 0 1190 192 369 714.6 0 369 193 75 714.6 0 75 194 55 714.6 0 55 195 86 714.6 0 86 196 79 714.6 0 79 197 123 714.6 0 123 198 117 714.6 0 117 199 86 714.6 0 86 200 86 714.6 0 86 201 63 714.6 0 63 202 75 714.6 0 75 203 97 714.6 0 97 204 159 714.6 0 159 205 141 714.6 0 141 206 157 714.6 0 157 207 127 714.6 0 127 208 150 714.6 0 150 209 94 714.6 0 94 210 79 714.6 0 79 211 98 714.6 0 98 212 96 714.6 0 96 213 103 714.6 0 103 214 155 714.6 0 155 215 116 714.6 0 116 216 247 714.6 0 247 217 308 714.6 0 308 218 330 714.6 0 330 219 126 714.6 0 126 220 78 714.6 0 78 221 106 714.6 0 106 222 66 714.6 0 66 223 85 714.6 0 85 224 86 714.6 0 86 225 156 714.6 0 156 226 203 714.6 0 203 227 220 714.6 0 220 228 237 714.6 0 237 229 361 714.6 0 361 230 403 714.6 0 403 231 108 714.6 0 108 232 112 714.6 0 112 233 122 714.6 0 122 234 124 714.6 0 124 235 98 714.6 0 98 236 114 714.6 0 114 237 138 714.6 0 138 238 106 714.6 0 106 239 103 714.6 0 103 240 90 714.6 0 90 241 98 714.6 0 98 242 39 714.6 0 39 243 91 714.6 0 91 244 121 714.6 0 121 245 153 714.6 0 153 246 97 714.6 0 97 247 124 714.6 0 124 248 96 714.6 0 96 249 91 714.6 0 91 250 75 714.6 0 75 251 42 714.6 0 42 252 69 714.6 0 69 253 68 714.6 0 68 254 124 714.6 0 124 255 115 714.6 0 115 256 108 714.6 0 108 257 140 714.6 0 140 258 97 714.6 0 97 259 150 714.6 0 150 260 523 714.6 0 523 261 463 714.6 0 463 262 277 714.6 0 277 263 106 714.6 0 106 264 124 714.6 0 124 265 145 714.6 0 145 266 212 714.6 0 212 267 154 714.6 0 154 268 155 714.6 0 155 269 59 714.6 0 59 270 108 714.6 0 108 271 85 714.6 0 85 272 86 714.6 0 86 273 77 714.6 0 77 274 89 714.6 0 89 275 113 714.6 0 113 276 130 714.6 0 130 277 156 714.6 0 156 278 125 714.6 0 125 279 98 714.6 0 98 280 104 714.6 0 104 281 318 714.6 0 318 282 112 714.6 0 112 283 77 714.6 0 77 284 91 714.6 0 91 285 171 714.6 0 171 286 146 714.6 0 146 287 394 714.6 0 394 288 790 714.6 0 790 289 394 714.6 0 394 290 96 714.6 0 96 291 70 714.6 0 70 292 58 714.6 0 58 293 91 714.6 0 91 294 140 714.6 0 140 295 142 714.6 0 142 296 106 714.6 0 106 297 90 714.6 0 90 298 94 714.6 0 94 299 58 714.6 0 58 300 59 714.6 0 59 301 74 714.6 0 74 Finished in 10.20 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 255,200 Reads with adapters: 60,689 (23.8%) Reads that were too short: 71 (0.0%) Reads written (passing filters): 60,618 (23.8%) Total basepairs processed: 58,945,732 bp Total written (filtered): 16,717,716 bp (28.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 60689 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 15 0.0 3 0 0 15 16 432 0.0 3 0 426 6 17 59884 0.0 3 58878 990 13 3 18 124 0.0 3 2 116 6 19 38 0.0 3 0 0 36 2 20 18 0.0 3 1 1 0 16 21 1 0.0 3 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 1 1 38 9 0.0 3 0 0 0 9 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 1 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 109 3 0.0 3 0 0 0 3 114 1 0.0 3 0 0 0 1 117 3 0.0 3 0 0 0 3 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 4 0.0 3 0 0 0 4 125 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 3 0.0 3 0 0 0 3 129 49 0.0 3 0 0 0 49 131 7 0.0 3 0 0 0 7 132 22 0.0 3 0 0 0 22 149 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 189 4 0.0 3 0 0 0 4 190 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 34 0.0 3 0 0 2 32 232 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.11 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 60,618 Reads with adapters: 729 (1.2%) Reads written (passing filters): 60,618 (100.0%) Total basepairs processed: 16,717,716 bp Total written (filtered): 16,715,516 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 729 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.1% C: 31.6% G: 21.5% T: 3.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 716 947.2 0 716 4 13 236.8 0 13