This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.90 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 53,152 (1.8%) Reads that were too short: 8,193 (0.3%) Reads written (passing filters): 44,959 (1.5%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,037,870 bp (0.9%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 53152 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1019 714.6 0 1019 7 2896 714.6 0 2896 8 4546 714.6 0 4546 9 61 714.6 0 61 10 41 714.6 0 41 11 48 714.6 0 48 12 54 714.6 0 54 13 36 714.6 0 36 14 39 714.6 0 39 15 48 714.6 0 48 16 45 714.6 0 45 17 43 714.6 0 43 18 60 714.6 0 60 19 83 714.6 0 83 20 69 714.6 0 69 21 54 714.6 0 54 22 43 714.6 0 43 23 52 714.6 0 52 24 54 714.6 0 54 25 31 714.6 0 31 26 71 714.6 0 71 27 75 714.6 0 75 28 42 714.6 0 42 29 48 714.6 0 48 30 52 714.6 0 52 31 48 714.6 0 48 32 59 714.6 0 59 33 54 714.6 0 54 34 56 714.6 0 56 35 66 714.6 0 66 36 59 714.6 0 59 37 49 714.6 0 49 38 76 714.6 0 76 39 70 714.6 0 70 40 272 714.6 0 272 41 60 714.6 0 60 42 44 714.6 0 44 43 56 714.6 0 56 44 62 714.6 0 62 45 74 714.6 0 74 46 68 714.6 0 68 47 46 714.6 0 46 48 43 714.6 0 43 49 44 714.6 0 44 50 53 714.6 0 53 51 45 714.6 0 45 52 36 714.6 0 36 53 64 714.6 0 64 54 46 714.6 0 46 55 36 714.6 0 36 56 38 714.6 0 38 57 48 714.6 0 48 58 54 714.6 0 54 59 55 714.6 0 55 60 104 714.6 0 104 61 44 714.6 0 44 62 50 714.6 0 50 63 50 714.6 0 50 64 42 714.6 0 42 65 60 714.6 0 60 66 34 714.6 0 34 67 39 714.6 0 39 68 57 714.6 0 57 69 52 714.6 0 52 70 44 714.6 0 44 71 55 714.6 0 55 72 39 714.6 0 39 73 54 714.6 0 54 74 50 714.6 0 50 75 34 714.6 0 34 76 77 714.6 0 77 77 156 714.6 0 156 78 101 714.6 0 101 79 50 714.6 0 50 80 44 714.6 0 44 81 32 714.6 0 32 82 39 714.6 0 39 83 47 714.6 0 47 84 50 714.6 0 50 85 46 714.6 0 46 86 32 714.6 0 32 87 33 714.6 0 33 88 32 714.6 0 32 89 35 714.6 0 35 90 54 714.6 0 54 91 44 714.6 0 44 92 43 714.6 0 43 93 78 714.6 0 78 94 163 714.6 0 163 95 87 714.6 0 87 96 179 714.6 0 179 97 296 714.6 0 296 98 288 714.6 0 288 99 55 714.6 0 55 100 56 714.6 0 56 101 34 714.6 0 34 102 38 714.6 0 38 103 33 714.6 0 33 104 32 714.6 0 32 105 42 714.6 0 42 106 33 714.6 0 33 107 40 714.6 0 40 108 37 714.6 0 37 109 27 714.6 0 27 110 36 714.6 0 36 111 44 714.6 0 44 112 32 714.6 0 32 113 38 714.6 0 38 114 79 714.6 0 79 115 45 714.6 0 45 116 36 714.6 0 36 117 47 714.6 0 47 118 29 714.6 0 29 119 24 714.6 0 24 120 35 714.6 0 35 121 21 714.6 0 21 122 46 714.6 0 46 123 47 714.6 0 47 124 20 714.6 0 20 125 33 714.6 0 33 126 45 714.6 0 45 127 29 714.6 0 29 128 77 714.6 0 77 129 48 714.6 0 48 130 258 714.6 0 258 131 239 714.6 0 239 132 182 714.6 0 182 133 42 714.6 0 42 134 43 714.6 0 43 135 64 714.6 0 64 136 48 714.6 0 48 137 72 714.6 0 72 138 58 714.6 0 58 139 29 714.6 0 29 140 51 714.6 0 51 141 28 714.6 0 28 142 63 714.6 0 63 143 64 714.6 0 64 144 121 714.6 0 121 145 43 714.6 0 43 146 35 714.6 0 35 147 34 714.6 0 34 148 25 714.6 0 25 149 30 714.6 0 30 150 44 714.6 0 44 151 54 714.6 0 54 152 60 714.6 0 60 153 56 714.6 0 56 154 67 714.6 0 67 155 46 714.6 0 46 156 35 714.6 0 35 157 57 714.6 0 57 158 68 714.6 0 68 159 58 714.6 0 58 160 42 714.6 0 42 161 43 714.6 0 43 162 85 714.6 0 85 163 67 714.6 0 67 164 111 714.6 0 111 165 128 714.6 0 128 166 204 714.6 0 204 167 288 714.6 0 288 168 895 714.6 0 895 169 20913 714.6 0 20913 170 2230 714.6 0 2230 171 1027 714.6 0 1027 172 38 714.6 0 38 173 47 714.6 0 47 174 38 714.6 0 38 175 49 714.6 0 49 176 33 714.6 0 33 177 26 714.6 0 26 178 40 714.6 0 40 179 46 714.6 0 46 180 58 714.6 0 58 181 32 714.6 0 32 182 50 714.6 0 50 183 27 714.6 0 27 184 41 714.6 0 41 185 55 714.6 0 55 186 52 714.6 0 52 187 27 714.6 0 27 188 31 714.6 0 31 189 32 714.6 0 32 190 37 714.6 0 37 191 43 714.6 0 43 192 80 714.6 0 80 193 154 714.6 0 154 194 85 714.6 0 85 195 52 714.6 0 52 196 25 714.6 0 25 197 50 714.6 0 50 198 47 714.6 0 47 199 26 714.6 0 26 200 42 714.6 0 42 201 46 714.6 0 46 202 46 714.6 0 46 203 143 714.6 0 143 204 111 714.6 0 111 205 87 714.6 0 87 206 61 714.6 0 61 207 29 714.6 0 29 208 30 714.6 0 30 209 29 714.6 0 29 210 42 714.6 0 42 211 44 714.6 0 44 212 46 714.6 0 46 213 55 714.6 0 55 214 37 714.6 0 37 215 71 714.6 0 71 216 48 714.6 0 48 217 61 714.6 0 61 218 47 714.6 0 47 219 38 714.6 0 38 220 39 714.6 0 39 221 33 714.6 0 33 222 56 714.6 0 56 223 62 714.6 0 62 224 92 714.6 0 92 225 45 714.6 0 45 226 36 714.6 0 36 227 67 714.6 0 67 228 41 714.6 0 41 229 40 714.6 0 40 230 46 714.6 0 46 231 70 714.6 0 70 232 51 714.6 0 51 233 66 714.6 0 66 234 44 714.6 0 44 235 78 714.6 0 78 236 96 714.6 0 96 237 150 714.6 0 150 238 82 714.6 0 82 239 68 714.6 0 68 240 42 714.6 0 42 241 159 714.6 0 159 242 41 714.6 0 41 243 37 714.6 0 37 244 40 714.6 0 40 245 43 714.6 0 43 246 53 714.6 0 53 247 36 714.6 0 36 248 27 714.6 0 27 249 32 714.6 0 32 250 34 714.6 0 34 251 39 714.6 0 39 252 35 714.6 0 35 253 43 714.6 0 43 254 55 714.6 0 55 255 59 714.6 0 59 256 43 714.6 0 43 257 42 714.6 0 42 258 103 714.6 0 103 259 47 714.6 0 47 260 39 714.6 0 39 261 86 714.6 0 86 262 69 714.6 0 69 263 53 714.6 0 53 264 36 714.6 0 36 265 54 714.6 0 54 266 54 714.6 0 54 267 65 714.6 0 65 268 44 714.6 0 44 269 72 714.6 0 72 270 100 714.6 0 100 271 299 714.6 0 299 272 574 714.6 0 574 273 547 714.6 0 547 274 1664 714.6 0 1664 275 49 714.6 0 49 276 42 714.6 0 42 277 41 714.6 0 41 278 52 714.6 0 52 279 36 714.6 0 36 280 33 714.6 0 33 281 45 714.6 0 45 282 36 714.6 0 36 283 53 714.6 0 53 284 43 714.6 0 43 285 52 714.6 0 52 286 43 714.6 0 43 287 37 714.6 0 37 288 53 714.6 0 53 289 40 714.6 0 40 290 42 714.6 0 42 291 36 714.6 0 36 292 61 714.6 0 61 293 39 714.6 0 39 294 44 714.6 0 44 295 37 714.6 0 37 296 40 714.6 0 40 297 65 714.6 0 65 298 32 714.6 0 32 299 28 714.6 0 28 300 25 714.6 0 25 301 36 714.6 0 36 Finished in 1.58 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 44,959 Reads with adapters: 3,490 (7.8%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 3,479 (7.7%) Total basepairs processed: 8,037,870 bp Total written (filtered): 961,632 bp (12.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3490 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 26 0.0 3 0 25 1 17 2686 0.0 3 2667 19 18 13 0.0 3 0 12 1 19 24 0.0 3 4 16 3 1 20 715 0.0 3 696 15 1 3 21 5 0.0 3 1 4 22 1 0.0 3 0 0 1 35 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 126 7 0.0 3 0 0 0 7 129 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 3,479 Reads with adapters: 209 (6.0%) Reads written (passing filters): 3,479 (100.0%) Total basepairs processed: 961,632 bp Total written (filtered): 961,004 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 209 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.5% C: 60.8% G: 14.4% T: 3.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 208 54.4 0 208 4 1 13.6 0 1