This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.46 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 94,809 (3.2%) Reads that were too short: 30,755 (1.1%) Reads written (passing filters): 64,054 (2.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 14,382,774 bp (1.6%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 94809 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 840 714.6 0 840 7 13987 714.6 0 13987 8 13935 714.6 0 13935 9 76 714.6 0 76 10 68 714.6 0 68 11 63 714.6 0 63 12 52 714.6 0 52 13 66 714.6 0 66 14 59 714.6 0 59 15 63 714.6 0 63 16 58 714.6 0 58 17 60 714.6 0 60 18 53 714.6 0 53 19 56 714.6 0 56 20 56 714.6 0 56 21 55 714.6 0 55 22 57 714.6 0 57 23 55 714.6 0 55 24 61 714.6 0 61 25 77 714.6 0 77 26 65 714.6 0 65 27 54 714.6 0 54 28 73 714.6 0 73 29 54 714.6 0 54 30 70 714.6 0 70 31 79 714.6 0 79 32 62 714.6 0 62 33 64 714.6 0 64 34 59 714.6 0 59 35 72 714.6 0 72 36 52 714.6 0 52 37 47 714.6 0 47 38 51 714.6 0 51 39 51 714.6 0 51 40 71 714.6 0 71 41 70 714.6 0 70 42 61 714.6 0 61 43 48 714.6 0 48 44 61 714.6 0 61 45 56 714.6 0 56 46 45 714.6 0 45 47 61 714.6 0 61 48 47 714.6 0 47 49 63 714.6 0 63 50 48 714.6 0 48 51 49 714.6 0 49 52 72 714.6 0 72 53 1030 714.6 0 1030 54 83 714.6 0 83 55 116 714.6 0 116 56 34 714.6 0 34 57 45 714.6 0 45 58 49 714.6 0 49 59 64 714.6 0 64 60 43 714.6 0 43 61 42 714.6 0 42 62 72 714.6 0 72 63 89 714.6 0 89 64 828 714.6 0 828 65 196 714.6 0 196 66 208 714.6 0 208 67 43 714.6 0 43 68 70 714.6 0 70 69 78 714.6 0 78 70 40 714.6 0 40 71 93 714.6 0 93 72 45 714.6 0 45 73 44 714.6 0 44 74 50 714.6 0 50 75 99 714.6 0 99 76 48 714.6 0 48 77 51 714.6 0 51 78 47 714.6 0 47 79 69 714.6 0 69 80 89 714.6 0 89 81 92 714.6 0 92 82 58 714.6 0 58 83 181 714.6 0 181 84 202 714.6 0 202 85 104 714.6 0 104 86 106 714.6 0 106 87 171 714.6 0 171 88 122 714.6 0 122 89 327 714.6 0 327 90 374 714.6 0 374 91 134 714.6 0 134 92 337 714.6 0 337 93 353 714.6 0 353 94 121 714.6 0 121 95 174 714.6 0 174 96 137 714.6 0 137 97 60 714.6 0 60 98 62 714.6 0 62 99 116 714.6 0 116 100 160 714.6 0 160 101 172 714.6 0 172 102 84 714.6 0 84 103 88 714.6 0 88 104 90 714.6 0 90 105 73 714.6 0 73 106 49 714.6 0 49 107 35 714.6 0 35 108 52 714.6 0 52 109 57 714.6 0 57 110 65 714.6 0 65 111 109 714.6 0 109 112 90 714.6 0 90 113 119 714.6 0 119 114 126 714.6 0 126 115 119 714.6 0 119 116 179 714.6 0 179 117 255 714.6 0 255 118 150 714.6 0 150 119 103 714.6 0 103 120 102 714.6 0 102 121 162 714.6 0 162 122 186 714.6 0 186 123 178 714.6 0 178 124 144 714.6 0 144 125 127 714.6 0 127 126 143 714.6 0 143 127 126 714.6 0 126 128 161 714.6 0 161 129 210 714.6 0 210 130 274 714.6 0 274 131 313 714.6 0 313 132 297 714.6 0 297 133 169 714.6 0 169 134 159 714.6 0 159 135 273 714.6 0 273 136 317 714.6 0 317 137 415 714.6 0 415 138 179 714.6 0 179 139 183 714.6 0 183 140 137 714.6 0 137 141 159 714.6 0 159 142 231 714.6 0 231 143 248 714.6 0 248 144 202 714.6 0 202 145 160 714.6 0 160 146 198 714.6 0 198 147 443 714.6 0 443 148 374 714.6 0 374 149 317 714.6 0 317 150 242 714.6 0 242 151 231 714.6 0 231 152 229 714.6 0 229 153 269 714.6 0 269 154 241 714.6 0 241 155 350 714.6 0 350 156 408 714.6 0 408 157 473 714.6 0 473 158 559 714.6 0 559 159 387 714.6 0 387 160 337 714.6 0 337 161 339 714.6 0 339 162 332 714.6 0 332 163 346 714.6 0 346 164 212 714.6 0 212 165 157 714.6 0 157 166 302 714.6 0 302 167 476 714.6 0 476 168 442 714.6 0 442 169 308 714.6 0 308 170 346 714.6 0 346 171 339 714.6 0 339 172 306 714.6 0 306 173 244 714.6 0 244 174 230 714.6 0 230 175 623 714.6 0 623 176 1101 714.6 0 1101 177 625 714.6 0 625 178 323 714.6 0 323 179 233 714.6 0 233 180 213 714.6 0 213 181 248 714.6 0 248 182 296 714.6 0 296 183 385 714.6 0 385 184 357 714.6 0 357 185 252 714.6 0 252 186 419 714.6 0 419 187 430 714.6 0 430 188 531 714.6 0 531 189 333 714.6 0 333 190 178 714.6 0 178 191 175 714.6 0 175 192 202 714.6 0 202 193 193 714.6 0 193 194 247 714.6 0 247 195 242 714.6 0 242 196 184 714.6 0 184 197 157 714.6 0 157 198 124 714.6 0 124 199 263 714.6 0 263 200 287 714.6 0 287 201 168 714.6 0 168 202 152 714.6 0 152 203 152 714.6 0 152 204 241 714.6 0 241 205 252 714.6 0 252 206 236 714.6 0 236 207 192 714.6 0 192 208 235 714.6 0 235 209 249 714.6 0 249 210 273 714.6 0 273 211 266 714.6 0 266 212 247 714.6 0 247 213 285 714.6 0 285 214 344 714.6 0 344 215 387 714.6 0 387 216 469 714.6 0 469 217 389 714.6 0 389 218 349 714.6 0 349 219 347 714.6 0 347 220 383 714.6 0 383 221 396 714.6 0 396 222 570 714.6 0 570 223 721 714.6 0 721 224 466 714.6 0 466 225 456 714.6 0 456 226 458 714.6 0 458 227 394 714.6 0 394 228 366 714.6 0 366 229 389 714.6 0 389 230 275 714.6 0 275 231 188 714.6 0 188 232 258 714.6 0 258 233 291 714.6 0 291 234 266 714.6 0 266 235 265 714.6 0 265 236 358 714.6 0 358 237 278 714.6 0 278 238 245 714.6 0 245 239 257 714.6 0 257 240 219 714.6 0 219 241 321 714.6 0 321 242 325 714.6 0 325 243 562 714.6 0 562 244 457 714.6 0 457 245 461 714.6 0 461 246 339 714.6 0 339 247 590 714.6 0 590 248 404 714.6 0 404 249 339 714.6 0 339 250 174 714.6 0 174 251 101 714.6 0 101 252 467 714.6 0 467 253 661 714.6 0 661 254 604 714.6 0 604 255 612 714.6 0 612 256 433 714.6 0 433 257 379 714.6 0 379 258 284 714.6 0 284 259 195 714.6 0 195 260 111 714.6 0 111 261 83 714.6 0 83 262 150 714.6 0 150 263 227 714.6 0 227 264 316 714.6 0 316 265 315 714.6 0 315 266 306 714.6 0 306 267 345 714.6 0 345 268 631 714.6 0 631 269 474 714.6 0 474 270 351 714.6 0 351 271 224 714.6 0 224 272 249 714.6 0 249 273 340 714.6 0 340 274 403 714.6 0 403 275 311 714.6 0 311 276 150 714.6 0 150 277 136 714.6 0 136 278 159 714.6 0 159 279 237 714.6 0 237 280 375 714.6 0 375 281 303 714.6 0 303 282 227 714.6 0 227 283 259 714.6 0 259 284 189 714.6 0 189 285 298 714.6 0 298 286 298 714.6 0 298 287 419 714.6 0 419 288 237 714.6 0 237 289 151 714.6 0 151 290 138 714.6 0 138 291 145 714.6 0 145 292 270 714.6 0 270 293 412 714.6 0 412 294 289 714.6 0 289 295 151 714.6 0 151 296 123 714.6 0 123 297 108 714.6 0 108 298 129 714.6 0 129 299 129 714.6 0 129 300 201 714.6 0 201 301 114 714.6 0 114 Finished in 2.94 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 64,054 Reads with adapters: 14,257 (22.3%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 14,233 (22.2%) Total basepairs processed: 14,382,774 bp Total written (filtered): 3,884,681 bp (27.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14257 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 7 0.0 3 0 0 7 19 137 0.0 3 0 122 7 3 5 20 13942 0.0 4 13505 360 59 14 4 21 42 0.0 4 7 32 2 0 1 22 3 0.0 4 1 0 2 23 87 0.0 4 74 0 0 11 2 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 45 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 140 3 0.0 4 0 0 0 0 3 146 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 1 258 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 289 5 0.0 4 0 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.51 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 14,233 Reads with adapters: 2,308 (16.2%) Reads written (passing filters): 14,233 (100.0%) Total basepairs processed: 3,884,681 bp Total written (filtered): 3,865,457 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2308 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.7% C: 4.0% G: 89.9% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 222.4 0 3 4 984 55.6 0 984 5 19 13.9 0 19 6 8 3.5 0 8 7 8 0.9 0 8 9 33 0.1 0 3 30 10 39 0.0 1 0 39 11 1207 0.0 1 9 1198 12 2 0.0 1 0 2 151 1 0.0 1 1 190 1 0.0 1 0 1 209 1 0.0 1 1 271 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.