This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.59 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 382,749 (13.1%) Reads that were too short: 166,471 (5.7%) Reads written (passing filters): 216,278 (7.4%) Total basepairs processed: 881,011,047 bp Total written (filtered): 44,931,690 bp (5.1%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 382749 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 550 714.6 0 550 7 12508 714.6 0 12508 8 1089 714.6 0 1089 9 41342 714.6 0 41342 10 138 714.6 0 138 11 59 714.6 0 59 12 66 714.6 0 66 13 72 714.6 0 72 14 73 714.6 0 73 15 65 714.6 0 65 16 76 714.6 0 76 17 48 714.6 0 48 18 58 714.6 0 58 19 52 714.6 0 52 20 63 714.6 0 63 21 76 714.6 0 76 22 76 714.6 0 76 23 67 714.6 0 67 24 62 714.6 0 62 25 70 714.6 0 70 26 53 714.6 0 53 27 60 714.6 0 60 28 68 714.6 0 68 29 76 714.6 0 76 30 80 714.6 0 80 31 57 714.6 0 57 32 73 714.6 0 73 33 53 714.6 0 53 34 68 714.6 0 68 35 83 714.6 0 83 36 119 714.6 0 119 37 119 714.6 0 119 38 222 714.6 0 222 39 47 714.6 0 47 40 54 714.6 0 54 41 46 714.6 0 46 42 40 714.6 0 40 43 55 714.6 0 55 44 50 714.6 0 50 45 56 714.6 0 56 46 47 714.6 0 47 47 32 714.6 0 32 48 39 714.6 0 39 49 47 714.6 0 47 50 47 714.6 0 47 51 44 714.6 0 44 52 38 714.6 0 38 53 32 714.6 0 32 54 82 714.6 0 82 55 92 714.6 0 92 56 15885 714.6 0 15885 57 1632 714.6 0 1632 58 48 714.6 0 48 59 55 714.6 0 55 60 53 714.6 0 53 61 43 714.6 0 43 62 57 714.6 0 57 63 32 714.6 0 32 64 43 714.6 0 43 65 89 714.6 0 89 66 120 714.6 0 120 67 59 714.6 0 59 68 88 714.6 0 88 69 163 714.6 0 163 70 231 714.6 0 231 71 136 714.6 0 136 72 523 714.6 0 523 73 545 714.6 0 545 74 579 714.6 0 579 75 1385 714.6 0 1385 76 1234 714.6 0 1234 77 1726 714.6 0 1726 78 2002 714.6 0 2002 79 783 714.6 0 783 80 1461 714.6 0 1461 81 1367 714.6 0 1367 82 1045 714.6 0 1045 83 482 714.6 0 482 84 765 714.6 0 765 85 651 714.6 0 651 86 669 714.6 0 669 87 591 714.6 0 591 88 1058 714.6 0 1058 89 863 714.6 0 863 90 1250 714.6 0 1250 91 693 714.6 0 693 92 880 714.6 0 880 93 1125 714.6 0 1125 94 1105 714.6 0 1105 95 862 714.6 0 862 96 1794 714.6 0 1794 97 2173 714.6 0 2173 98 2793 714.6 0 2793 99 2342 714.6 0 2342 100 1263 714.6 0 1263 101 987 714.6 0 987 102 561 714.6 0 561 103 375 714.6 0 375 104 304 714.6 0 304 105 590 714.6 0 590 106 2435 714.6 0 2435 107 1876 714.6 0 1876 108 1575 714.6 0 1575 109 1141 714.6 0 1141 110 1655 714.6 0 1655 111 1508 714.6 0 1508 112 1342 714.6 0 1342 113 1129 714.6 0 1129 114 1110 714.6 0 1110 115 777 714.6 0 777 116 489 714.6 0 489 117 508 714.6 0 508 118 532 714.6 0 532 119 1687 714.6 0 1687 120 1687 714.6 0 1687 121 931 714.6 0 931 122 586 714.6 0 586 123 1442 714.6 0 1442 124 961 714.6 0 961 125 870 714.6 0 870 126 832 714.6 0 832 127 779 714.6 0 779 128 1145 714.6 0 1145 129 1141 714.6 0 1141 130 819 714.6 0 819 131 814 714.6 0 814 132 1193 714.6 0 1193 133 1003 714.6 0 1003 134 1027 714.6 0 1027 135 744 714.6 0 744 136 670 714.6 0 670 137 662 714.6 0 662 138 831 714.6 0 831 139 2497 714.6 0 2497 140 1915 714.6 0 1915 141 1031 714.6 0 1031 142 462 714.6 0 462 143 613 714.6 0 613 144 553 714.6 0 553 145 586 714.6 0 586 146 842 714.6 0 842 147 673 714.6 0 673 148 732 714.6 0 732 149 637 714.6 0 637 150 608 714.6 0 608 151 394 714.6 0 394 152 435 714.6 0 435 153 580 714.6 0 580 154 617 714.6 0 617 155 782 714.6 0 782 156 638 714.6 0 638 157 735 714.6 0 735 158 878 714.6 0 878 159 3902 714.6 0 3902 160 15172 714.6 0 15172 161 5976 714.6 0 5976 162 488 714.6 0 488 163 618 714.6 0 618 164 639 714.6 0 639 165 801 714.6 0 801 166 566 714.6 0 566 167 638 714.6 0 638 168 671 714.6 0 671 169 421 714.6 0 421 170 312 714.6 0 312 171 327 714.6 0 327 172 445 714.6 0 445 173 678 714.6 0 678 174 797 714.6 0 797 175 512 714.6 0 512 176 520 714.6 0 520 177 398 714.6 0 398 178 498 714.6 0 498 179 484 714.6 0 484 180 633 714.6 0 633 181 566 714.6 0 566 182 448 714.6 0 448 183 636 714.6 0 636 184 754 714.6 0 754 185 793 714.6 0 793 186 487 714.6 0 487 187 519 714.6 0 519 188 587 714.6 0 587 189 528 714.6 0 528 190 528 714.6 0 528 191 574 714.6 0 574 192 740 714.6 0 740 193 682 714.6 0 682 194 845 714.6 0 845 195 1014 714.6 0 1014 196 938 714.6 0 938 197 592 714.6 0 592 198 471 714.6 0 471 199 479 714.6 0 479 200 1106 714.6 0 1106 201 1237 714.6 0 1237 202 864 714.6 0 864 203 1018 714.6 0 1018 204 1142 714.6 0 1142 205 1018 714.6 0 1018 206 1328 714.6 0 1328 207 1913 714.6 0 1913 208 1446 714.6 0 1446 209 1365 714.6 0 1365 210 1208 714.6 0 1208 211 1125 714.6 0 1125 212 981 714.6 0 981 213 1286 714.6 0 1286 214 746 714.6 0 746 215 628 714.6 0 628 216 496 714.6 0 496 217 454 714.6 0 454 218 496 714.6 0 496 219 774 714.6 0 774 220 946 714.6 0 946 221 1312 714.6 0 1312 222 659 714.6 0 659 223 780 714.6 0 780 224 867 714.6 0 867 225 632 714.6 0 632 226 470 714.6 0 470 227 526 714.6 0 526 228 515 714.6 0 515 229 833 714.6 0 833 230 819 714.6 0 819 231 841 714.6 0 841 232 1218 714.6 0 1218 233 1671 714.6 0 1671 234 1398 714.6 0 1398 235 602 714.6 0 602 236 585 714.6 0 585 237 730 714.6 0 730 238 799 714.6 0 799 239 557 714.6 0 557 240 1096 714.6 0 1096 241 1107 714.6 0 1107 242 1221 714.6 0 1221 243 1008 714.6 0 1008 244 627 714.6 0 627 245 443 714.6 0 443 246 261 714.6 0 261 247 299 714.6 0 299 248 453 714.6 0 453 249 895 714.6 0 895 250 1452 714.6 0 1452 251 1430 714.6 0 1430 252 1172 714.6 0 1172 253 2433 714.6 0 2433 254 6327 714.6 0 6327 255 38914 714.6 0 38914 256 33173 714.6 0 33173 257 9335 714.6 0 9335 258 565 714.6 0 565 259 709 714.6 0 709 260 1316 714.6 0 1316 261 1858 714.6 0 1858 262 1467 714.6 0 1467 263 1024 714.6 0 1024 264 429 714.6 0 429 265 539 714.6 0 539 266 429 714.6 0 429 267 328 714.6 0 328 268 384 714.6 0 384 269 644 714.6 0 644 270 639 714.6 0 639 271 758 714.6 0 758 272 757 714.6 0 757 273 596 714.6 0 596 274 350 714.6 0 350 275 545 714.6 0 545 276 637 714.6 0 637 277 548 714.6 0 548 278 472 714.6 0 472 279 530 714.6 0 530 280 489 714.6 0 489 281 532 714.6 0 532 282 1095 714.6 0 1095 283 1170 714.6 0 1170 284 810 714.6 0 810 285 306 714.6 0 306 286 236 714.6 0 236 287 325 714.6 0 325 288 486 714.6 0 486 289 612 714.6 0 612 290 761 714.6 0 761 291 457 714.6 0 457 292 413 714.6 0 413 293 440 714.6 0 440 294 446 714.6 0 446 295 611 714.6 0 611 296 833 714.6 0 833 297 889 714.6 0 889 298 758 714.6 0 758 299 936 714.6 0 936 300 791 714.6 0 791 301 857 714.6 0 857 Finished in 8.93 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 216,278 Reads with adapters: 12,548 (5.8%) Reads that were too short: 91 (0.0%) Reads written (passing filters): 12,457 (5.8%) Total basepairs processed: 44,931,690 bp Total written (filtered): 3,406,632 bp (7.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12548 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 93 0.0 3 0 87 3 2 1 20 12257 0.0 4 12006 203 27 18 3 21 38 0.0 4 4 30 0 2 2 22 3 0.0 4 0 1 1 0 1 23 10 0.0 4 0 0 0 9 1 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 0 1 28 2 0.0 4 0 0 0 0 2 32 1 0.0 4 0 0 0 0 1 33 2 0.0 4 0 0 0 0 2 35 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 45 2 0.0 4 0 0 0 0 2 47 2 0.0 4 0 0 0 0 2 50 2 0.0 4 0 0 0 0 2 54 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 63 4 0.0 4 0 0 0 0 4 64 2 0.0 4 0 0 0 0 2 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 2 0.0 4 0 0 0 0 2 72 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 3 0.0 4 0 0 0 0 3 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 2 0.0 4 0 0 0 0 2 94 2 0.0 4 0 0 0 0 2 96 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 2 0.0 4 0 0 0 0 2 104 1 0.0 4 0 0 0 1 106 3 0.0 4 0 0 0 0 3 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 1 111 3 0.0 4 0 0 0 1 2 112 3 0.0 4 0 0 0 1 2 115 1 0.0 4 0 0 0 0 1 116 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 132 2 0.0 4 0 0 0 0 2 134 1 0.0 4 0 0 0 0 1 138 4 0.0 4 0 0 0 0 4 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 143 2 0.0 4 0 0 0 0 2 148 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 165 2 0.0 4 0 0 0 1 1 168 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 200 2 0.0 4 0 0 0 0 2 201 2 0.0 4 0 0 0 0 2 202 1 0.0 4 0 0 0 0 1 204 2 0.0 4 0 0 0 0 2 205 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 1 228 6 0.0 4 0 0 0 1 5 241 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 4 0.0 4 0 0 0 0 4 260 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.43 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 12,457 Reads with adapters: 614 (4.9%) Reads written (passing filters): 12,457 (100.0%) Total basepairs processed: 3,406,632 bp Total written (filtered): 3,396,839 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 614 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.1% C: 6.8% G: 87.8% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 194.6 0 8 4 1 48.7 0 1 9 1 0.0 0 0 1 11 570 0.0 1 3 567 12 14 0.0 1 2 12 13 2 0.0 1 1 1 152 12 0.0 1 10 2 153 1 0.0 1 1 261 4 0.0 1 4 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.