This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.56 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 38,092 (1.3%) Reads that were too short: 2,995 (0.1%) Reads written (passing filters): 35,097 (1.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 9,538,919 bp (1.1%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 38092 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1990 714.6 0 1990 7 1737 714.6 0 1737 8 13598 714.6 0 13598 9 10565 714.6 0 10565 10 55 714.6 0 55 11 35 714.6 0 35 12 35 714.6 0 35 13 35 714.6 0 35 14 34 714.6 0 34 15 43 714.6 0 43 16 34 714.6 0 34 17 36 714.6 0 36 18 40 714.6 0 40 19 36 714.6 0 36 20 32 714.6 0 32 21 26 714.6 0 26 22 47 714.6 0 47 23 35 714.6 0 35 24 31 714.6 0 31 25 38 714.6 0 38 26 35 714.6 0 35 27 39 714.6 0 39 28 40 714.6 0 40 29 33 714.6 0 33 30 44 714.6 0 44 31 36 714.6 0 36 32 29 714.6 0 29 33 39 714.6 0 39 34 26 714.6 0 26 35 34 714.6 0 34 36 31 714.6 0 31 37 36 714.6 0 36 38 34 714.6 0 34 39 38 714.6 0 38 40 26 714.6 0 26 41 36 714.6 0 36 42 37 714.6 0 37 43 33 714.6 0 33 44 29 714.6 0 29 45 30 714.6 0 30 46 27 714.6 0 27 47 34 714.6 0 34 48 93 714.6 0 93 49 60 714.6 0 60 50 208 714.6 0 208 51 37 714.6 0 37 52 29 714.6 0 29 53 32 714.6 0 32 54 23 714.6 0 23 55 21 714.6 0 21 56 28 714.6 0 28 57 21 714.6 0 21 58 21 714.6 0 21 59 20 714.6 0 20 60 26 714.6 0 26 61 31 714.6 0 31 62 21 714.6 0 21 63 66 714.6 0 66 64 27 714.6 0 27 65 24 714.6 0 24 66 18 714.6 0 18 67 19 714.6 0 19 68 21 714.6 0 21 69 8 714.6 0 8 70 22 714.6 0 22 71 23 714.6 0 23 72 17 714.6 0 17 73 16 714.6 0 16 74 22 714.6 0 22 75 17 714.6 0 17 76 15 714.6 0 15 77 15 714.6 0 15 78 23 714.6 0 23 79 10 714.6 0 10 80 17 714.6 0 17 81 21 714.6 0 21 82 29 714.6 0 29 83 29 714.6 0 29 84 28 714.6 0 28 85 38 714.6 0 38 86 19 714.6 0 19 87 10 714.6 0 10 88 15 714.6 0 15 89 23 714.6 0 23 90 18 714.6 0 18 91 12 714.6 0 12 92 13 714.6 0 13 93 12 714.6 0 12 94 20 714.6 0 20 95 31 714.6 0 31 96 106 714.6 0 106 97 23 714.6 0 23 98 24 714.6 0 24 99 16 714.6 0 16 100 15 714.6 0 15 101 18 714.6 0 18 102 27 714.6 0 27 103 18 714.6 0 18 104 12 714.6 0 12 105 10 714.6 0 10 106 7 714.6 0 7 107 7 714.6 0 7 108 7 714.6 0 7 109 14 714.6 0 14 110 9 714.6 0 9 111 11 714.6 0 11 112 10 714.6 0 10 113 17 714.6 0 17 114 18 714.6 0 18 115 10 714.6 0 10 116 10 714.6 0 10 117 12 714.6 0 12 118 30 714.6 0 30 119 31 714.6 0 31 120 27 714.6 0 27 121 14 714.6 0 14 122 20 714.6 0 20 123 14 714.6 0 14 124 17 714.6 0 17 125 10 714.6 0 10 126 17 714.6 0 17 127 48 714.6 0 48 128 18 714.6 0 18 129 19 714.6 0 19 130 17 714.6 0 17 131 9 714.6 0 9 132 21 714.6 0 21 133 47 714.6 0 47 134 25 714.6 0 25 135 23 714.6 0 23 136 23 714.6 0 23 137 26 714.6 0 26 138 25 714.6 0 25 139 29 714.6 0 29 140 22 714.6 0 22 141 38 714.6 0 38 142 26 714.6 0 26 143 36 714.6 0 36 144 27 714.6 0 27 145 46 714.6 0 46 146 336 714.6 0 336 147 1828 714.6 0 1828 148 92 714.6 0 92 149 22 714.6 0 22 150 21 714.6 0 21 151 11 714.6 0 11 152 12 714.6 0 12 153 15 714.6 0 15 154 8 714.6 0 8 155 15 714.6 0 15 156 14 714.6 0 14 157 17 714.6 0 17 158 16 714.6 0 16 159 19 714.6 0 19 160 13 714.6 0 13 161 14 714.6 0 14 162 10 714.6 0 10 163 11 714.6 0 11 164 16 714.6 0 16 165 18 714.6 0 18 166 15 714.6 0 15 167 17 714.6 0 17 168 30 714.6 0 30 169 29 714.6 0 29 170 22 714.6 0 22 171 17 714.6 0 17 172 23 714.6 0 23 173 18 714.6 0 18 174 11 714.6 0 11 175 15 714.6 0 15 176 21 714.6 0 21 177 14 714.6 0 14 178 10 714.6 0 10 179 19 714.6 0 19 180 22 714.6 0 22 181 19 714.6 0 19 182 24 714.6 0 24 183 20 714.6 0 20 184 17 714.6 0 17 185 25 714.6 0 25 186 19 714.6 0 19 187 13 714.6 0 13 188 18 714.6 0 18 189 28 714.6 0 28 190 26 714.6 0 26 191 15 714.6 0 15 192 19 714.6 0 19 193 34 714.6 0 34 194 28 714.6 0 28 195 91 714.6 0 91 196 85 714.6 0 85 197 47 714.6 0 47 198 21 714.6 0 21 199 21 714.6 0 21 200 19 714.6 0 19 201 14 714.6 0 14 202 7 714.6 0 7 203 11 714.6 0 11 204 17 714.6 0 17 205 16 714.6 0 16 206 16 714.6 0 16 207 20 714.6 0 20 208 11 714.6 0 11 209 22 714.6 0 22 210 17 714.6 0 17 211 10 714.6 0 10 212 25 714.6 0 25 213 38 714.6 0 38 214 40 714.6 0 40 215 39 714.6 0 39 216 30 714.6 0 30 217 22 714.6 0 22 218 43 714.6 0 43 219 39 714.6 0 39 220 28 714.6 0 28 221 21 714.6 0 21 222 15 714.6 0 15 223 13 714.6 0 13 224 23 714.6 0 23 225 23 714.6 0 23 226 16 714.6 0 16 227 27 714.6 0 27 228 19 714.6 0 19 229 34 714.6 0 34 230 31 714.6 0 31 231 22 714.6 0 22 232 12 714.6 0 12 233 16 714.6 0 16 234 25 714.6 0 25 235 34 714.6 0 34 236 85 714.6 0 85 237 35 714.6 0 35 238 61 714.6 0 61 239 47 714.6 0 47 240 47 714.6 0 47 241 20 714.6 0 20 242 23 714.6 0 23 243 11 714.6 0 11 244 37 714.6 0 37 245 38 714.6 0 38 246 25 714.6 0 25 247 49 714.6 0 49 248 20 714.6 0 20 249 9 714.6 0 9 250 14 714.6 0 14 251 13 714.6 0 13 252 14 714.6 0 14 253 10 714.6 0 10 254 22 714.6 0 22 255 26 714.6 0 26 256 40 714.6 0 40 257 35 714.6 0 35 258 19 714.6 0 19 259 20 714.6 0 20 260 121 714.6 0 121 261 25 714.6 0 25 262 23 714.6 0 23 263 22 714.6 0 22 264 12 714.6 0 12 265 19 714.6 0 19 266 18 714.6 0 18 267 22 714.6 0 22 268 21 714.6 0 21 269 13 714.6 0 13 270 9 714.6 0 9 271 12 714.6 0 12 272 17 714.6 0 17 273 12 714.6 0 12 274 18 714.6 0 18 275 26 714.6 0 26 276 23 714.6 0 23 277 14 714.6 0 14 278 22 714.6 0 22 279 46 714.6 0 46 280 22 714.6 0 22 281 18 714.6 0 18 282 15 714.6 0 15 283 28 714.6 0 28 284 33 714.6 0 33 285 26 714.6 0 26 286 48 714.6 0 48 287 159 714.6 0 159 288 97 714.6 0 97 289 21 714.6 0 21 290 25 714.6 0 25 291 12 714.6 0 12 292 21 714.6 0 21 293 46 714.6 0 46 294 44 714.6 0 44 295 20 714.6 0 20 296 36 714.6 0 36 297 35 714.6 0 35 298 201 714.6 0 201 299 23 714.6 0 23 300 18 714.6 0 18 301 50 714.6 0 50 Finished in 1.64 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 35,097 Reads with adapters: 15,075 (43.0%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 15,061 (42.9%) Total basepairs processed: 9,538,919 bp Total written (filtered): 4,153,788 bp (43.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15075 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 9 0.0 3 0 0 5 4 16 103 0.0 3 0 99 2 2 17 13108 0.0 3 12925 181 2 18 66 0.0 3 1 41 18 6 19 1708 0.0 3 1649 23 7 29 20 32 0.0 3 0 2 5 25 21 4 0.0 3 0 0 1 3 22 2 0.0 3 0 0 0 2 24 2 0.0 3 0 0 0 2 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 3 0.0 3 0 0 0 3 32 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 5 0.0 3 0 0 0 5 46 1 0.0 3 0 0 1 48 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 12 0.0 3 0 0 0 12 234 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.52 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 15,061 Reads with adapters: 151 (1.0%) Reads written (passing filters): 15,061 (100.0%) Total basepairs processed: 4,153,788 bp Total written (filtered): 4,153,332 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 151 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.9% C: 27.8% G: 40.4% T: 13.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 148 235.3 0 148 4 3 58.8 0 3