This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.55 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 84,425 (2.9%) Reads that were too short: 29,172 (1.0%) Reads written (passing filters): 55,253 (1.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,405,591 bp (1.0%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 84425 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 187 714.6 0 187 7 54 714.6 0 54 8 137 714.6 0 137 9 1564 714.6 0 1564 10 24 714.6 0 24 11 24 714.6 0 24 12 16 714.6 0 16 13 25 714.6 0 25 14 21 714.6 0 21 15 23 714.6 0 23 16 31 714.6 0 31 17 29 714.6 0 29 18 34 714.6 0 34 19 37 714.6 0 37 20 46 714.6 0 46 21 242 714.6 0 242 22 56 714.6 0 56 23 35 714.6 0 35 24 29 714.6 0 29 25 40 714.6 0 40 26 1551 714.6 0 1551 27 309 714.6 0 309 28 30 714.6 0 30 29 34 714.6 0 34 30 39 714.6 0 39 31 27 714.6 0 27 32 37 714.6 0 37 33 139 714.6 0 139 34 73 714.6 0 73 35 42 714.6 0 42 36 30 714.6 0 30 37 27 714.6 0 27 38 36 714.6 0 36 39 29 714.6 0 29 40 31 714.6 0 31 41 33 714.6 0 33 42 32 714.6 0 32 43 29 714.6 0 29 44 48 714.6 0 48 45 32 714.6 0 32 46 26 714.6 0 26 47 46 714.6 0 46 48 23 714.6 0 23 49 22 714.6 0 22 50 32 714.6 0 32 51 23 714.6 0 23 52 31 714.6 0 31 53 41 714.6 0 41 54 27 714.6 0 27 55 32 714.6 0 32 56 23 714.6 0 23 57 29 714.6 0 29 58 37 714.6 0 37 59 31 714.6 0 31 60 41 714.6 0 41 61 26 714.6 0 26 62 26 714.6 0 26 63 34 714.6 0 34 64 41 714.6 0 41 65 50 714.6 0 50 66 30 714.6 0 30 67 28 714.6 0 28 68 38 714.6 0 38 69 107 714.6 0 107 70 34 714.6 0 34 71 37 714.6 0 37 72 24 714.6 0 24 73 19 714.6 0 19 74 54 714.6 0 54 75 60 714.6 0 60 76 78 714.6 0 78 77 64 714.6 0 64 78 56 714.6 0 56 79 89 714.6 0 89 80 318 714.6 0 318 81 193 714.6 0 193 82 192 714.6 0 192 83 176 714.6 0 176 84 192 714.6 0 192 85 93 714.6 0 93 86 177 714.6 0 177 87 161 714.6 0 161 88 204 714.6 0 204 89 153 714.6 0 153 90 117 714.6 0 117 91 75 714.6 0 75 92 67 714.6 0 67 93 127 714.6 0 127 94 105 714.6 0 105 95 165 714.6 0 165 96 274 714.6 0 274 97 339 714.6 0 339 98 114 714.6 0 114 99 115 714.6 0 115 100 139 714.6 0 139 101 97 714.6 0 97 102 85 714.6 0 85 103 78 714.6 0 78 104 58 714.6 0 58 105 59 714.6 0 59 106 43 714.6 0 43 107 45 714.6 0 45 108 222 714.6 0 222 109 28 714.6 0 28 110 47 714.6 0 47 111 81 714.6 0 81 112 80 714.6 0 80 113 140 714.6 0 140 114 116 714.6 0 116 115 91 714.6 0 91 116 104 714.6 0 104 117 586 714.6 0 586 118 408 714.6 0 408 119 281 714.6 0 281 120 103 714.6 0 103 121 121 714.6 0 121 122 131 714.6 0 131 123 120 714.6 0 120 124 100 714.6 0 100 125 96 714.6 0 96 126 123 714.6 0 123 127 152 714.6 0 152 128 138 714.6 0 138 129 121 714.6 0 121 130 131 714.6 0 131 131 218 714.6 0 218 132 197 714.6 0 197 133 283 714.6 0 283 134 179 714.6 0 179 135 153 714.6 0 153 136 200 714.6 0 200 137 173 714.6 0 173 138 141 714.6 0 141 139 185 714.6 0 185 140 164 714.6 0 164 141 122 714.6 0 122 142 129 714.6 0 129 143 132 714.6 0 132 144 82 714.6 0 82 145 112 714.6 0 112 146 148 714.6 0 148 147 152 714.6 0 152 148 126 714.6 0 126 149 109 714.6 0 109 150 109 714.6 0 109 151 83 714.6 0 83 152 98 714.6 0 98 153 115 714.6 0 115 154 118 714.6 0 118 155 101 714.6 0 101 156 265 714.6 0 265 157 197 714.6 0 197 158 387 714.6 0 387 159 123 714.6 0 123 160 144 714.6 0 144 161 152 714.6 0 152 162 175 714.6 0 175 163 198 714.6 0 198 164 155 714.6 0 155 165 293 714.6 0 293 166 173 714.6 0 173 167 211 714.6 0 211 168 255 714.6 0 255 169 257 714.6 0 257 170 220 714.6 0 220 171 225 714.6 0 225 172 190 714.6 0 190 173 197 714.6 0 197 174 252 714.6 0 252 175 426 714.6 0 426 176 594 714.6 0 594 177 1260 714.6 0 1260 178 2116 714.6 0 2116 179 17631 714.6 0 17631 180 1435 714.6 0 1435 181 1723 714.6 0 1723 182 1421 714.6 0 1421 183 583 714.6 0 583 184 1874 714.6 0 1874 185 361 714.6 0 361 186 141 714.6 0 141 187 171 714.6 0 171 188 218 714.6 0 218 189 146 714.6 0 146 190 84 714.6 0 84 191 90 714.6 0 90 192 117 714.6 0 117 193 125 714.6 0 125 194 300 714.6 0 300 195 470 714.6 0 470 196 664 714.6 0 664 197 443 714.6 0 443 198 310 714.6 0 310 199 160 714.6 0 160 200 269 714.6 0 269 201 321 714.6 0 321 202 333 714.6 0 333 203 321 714.6 0 321 204 171 714.6 0 171 205 120 714.6 0 120 206 105 714.6 0 105 207 134 714.6 0 134 208 127 714.6 0 127 209 115 714.6 0 115 210 99 714.6 0 99 211 88 714.6 0 88 212 107 714.6 0 107 213 141 714.6 0 141 214 143 714.6 0 143 215 161 714.6 0 161 216 173 714.6 0 173 217 136 714.6 0 136 218 90 714.6 0 90 219 100 714.6 0 100 220 175 714.6 0 175 221 114 714.6 0 114 222 113 714.6 0 113 223 147 714.6 0 147 224 200 714.6 0 200 225 163 714.6 0 163 226 155 714.6 0 155 227 109 714.6 0 109 228 127 714.6 0 127 229 209 714.6 0 209 230 144 714.6 0 144 231 112 714.6 0 112 232 111 714.6 0 111 233 96 714.6 0 96 234 93 714.6 0 93 235 121 714.6 0 121 236 157 714.6 0 157 237 116 714.6 0 116 238 224 714.6 0 224 239 1821 714.6 0 1821 240 393 714.6 0 393 241 717 714.6 0 717 242 106 714.6 0 106 243 139 714.6 0 139 244 105 714.6 0 105 245 96 714.6 0 96 246 128 714.6 0 128 247 153 714.6 0 153 248 195 714.6 0 195 249 204 714.6 0 204 250 145 714.6 0 145 251 93 714.6 0 93 252 100 714.6 0 100 253 104 714.6 0 104 254 152 714.6 0 152 255 99 714.6 0 99 256 136 714.6 0 136 257 189 714.6 0 189 258 318 714.6 0 318 259 643 714.6 0 643 260 1143 714.6 0 1143 261 1384 714.6 0 1384 262 293 714.6 0 293 263 371 714.6 0 371 264 883 714.6 0 883 265 1201 714.6 0 1201 266 1271 714.6 0 1271 267 755 714.6 0 755 268 161 714.6 0 161 269 145 714.6 0 145 270 84 714.6 0 84 271 38 714.6 0 38 272 44 714.6 0 44 273 53 714.6 0 53 274 115 714.6 0 115 275 182 714.6 0 182 276 129 714.6 0 129 277 70 714.6 0 70 278 73 714.6 0 73 279 135 714.6 0 135 280 135 714.6 0 135 281 152 714.6 0 152 282 82 714.6 0 82 283 65 714.6 0 65 284 70 714.6 0 70 285 54 714.6 0 54 286 117 714.6 0 117 287 198 714.6 0 198 288 162 714.6 0 162 289 156 714.6 0 156 290 94 714.6 0 94 291 490 714.6 0 490 292 2483 714.6 0 2483 293 2287 714.6 0 2287 294 877 714.6 0 877 295 914 714.6 0 914 296 279 714.6 0 279 297 208 714.6 0 208 298 97 714.6 0 97 299 94 714.6 0 94 300 162 714.6 0 162 301 80 714.6 0 80 Finished in 1.96 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 55,253 Reads with adapters: 1,607 (2.9%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 1,592 (2.9%) Total basepairs processed: 8,405,591 bp Total written (filtered): 432,606 bp (5.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1607 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 0 1 19 14 0.0 3 0 11 1 0 2 20 1564 0.0 4 1454 85 17 4 4 21 5 0.0 4 0 5 23 2 0.0 4 1 0 0 1 27 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 40 2 0.0 4 0 0 0 0 2 62 2 0.0 4 0 0 0 0 2 66 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 79 2 0.0 4 0 0 0 0 2 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 1 111 2 0.0 4 0 0 0 2 129 1 0.0 4 0 0 0 0 1 133 2 0.0 4 0 0 0 0 2 155 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 1,592 Reads with adapters: 86 (5.4%) Reads written (passing filters): 1,592 (100.0%) Total basepairs processed: 432,606 bp Total written (filtered): 430,978 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 86 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.3% C: 5.8% G: 83.7% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 18 0.0 0 3 15 10 35 0.0 1 4 31 11 29 0.0 1 0 29 13 1 0.0 1 0 1 257 2 0.0 1 2 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.