This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.93 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 54,971 (1.9%) Reads that were too short: 10,983 (0.4%) Reads written (passing filters): 43,988 (1.5%) Total basepairs processed: 881,011,047 bp Total written (filtered): 11,199,806 bp (1.3%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 54971 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2651 714.6 0 2651 7 2242 714.6 0 2242 8 16742 714.6 0 16742 9 86 714.6 0 86 10 71 714.6 0 71 11 46 714.6 0 46 12 44 714.6 0 44 13 50 714.6 0 50 14 49 714.6 0 49 15 45 714.6 0 45 16 42 714.6 0 42 17 50 714.6 0 50 18 27 714.6 0 27 19 36 714.6 0 36 20 60 714.6 0 60 21 41 714.6 0 41 22 35 714.6 0 35 23 34 714.6 0 34 24 48 714.6 0 48 25 46 714.6 0 46 26 48 714.6 0 48 27 42 714.6 0 42 28 41 714.6 0 41 29 52 714.6 0 52 30 52 714.6 0 52 31 37 714.6 0 37 32 39 714.6 0 39 33 56 714.6 0 56 34 44 714.6 0 44 35 47 714.6 0 47 36 27 714.6 0 27 37 44 714.6 0 44 38 41 714.6 0 41 39 41 714.6 0 41 40 50 714.6 0 50 41 38 714.6 0 38 42 67 714.6 0 67 43 34 714.6 0 34 44 40 714.6 0 40 45 42 714.6 0 42 46 70 714.6 0 70 47 40 714.6 0 40 48 46 714.6 0 46 49 55 714.6 0 55 50 39 714.6 0 39 51 30 714.6 0 30 52 48 714.6 0 48 53 60 714.6 0 60 54 50 714.6 0 50 55 64 714.6 0 64 56 55 714.6 0 55 57 65 714.6 0 65 58 67 714.6 0 67 59 43 714.6 0 43 60 157 714.6 0 157 61 105 714.6 0 105 62 9528 714.6 0 9528 63 449 714.6 0 449 64 718 714.6 0 718 65 36 714.6 0 36 66 120 714.6 0 120 67 57 714.6 0 57 68 48 714.6 0 48 69 39 714.6 0 39 70 45 714.6 0 45 71 57 714.6 0 57 72 53 714.6 0 53 73 269 714.6 0 269 74 296 714.6 0 296 75 651 714.6 0 651 76 40 714.6 0 40 77 72 714.6 0 72 78 75 714.6 0 75 79 77 714.6 0 77 80 28 714.6 0 28 81 36 714.6 0 36 82 29 714.6 0 29 83 31 714.6 0 31 84 37 714.6 0 37 85 30 714.6 0 30 86 30 714.6 0 30 87 32 714.6 0 32 88 35 714.6 0 35 89 48 714.6 0 48 90 27 714.6 0 27 91 30 714.6 0 30 92 31 714.6 0 31 93 37 714.6 0 37 94 44 714.6 0 44 95 35 714.6 0 35 96 32 714.6 0 32 97 51 714.6 0 51 98 34 714.6 0 34 99 32 714.6 0 32 100 38 714.6 0 38 101 39 714.6 0 39 102 34 714.6 0 34 103 46 714.6 0 46 104 44 714.6 0 44 105 50 714.6 0 50 106 89 714.6 0 89 107 224 714.6 0 224 108 52 714.6 0 52 109 47 714.6 0 47 110 34 714.6 0 34 111 37 714.6 0 37 112 42 714.6 0 42 113 26 714.6 0 26 114 46 714.6 0 46 115 65 714.6 0 65 116 50 714.6 0 50 117 71 714.6 0 71 118 58 714.6 0 58 119 42 714.6 0 42 120 80 714.6 0 80 121 42 714.6 0 42 122 43 714.6 0 43 123 49 714.6 0 49 124 32 714.6 0 32 125 49 714.6 0 49 126 50 714.6 0 50 127 94 714.6 0 94 128 417 714.6 0 417 129 241 714.6 0 241 130 123 714.6 0 123 131 55 714.6 0 55 132 55 714.6 0 55 133 42 714.6 0 42 134 160 714.6 0 160 135 152 714.6 0 152 136 232 714.6 0 232 137 141 714.6 0 141 138 37 714.6 0 37 139 67 714.6 0 67 140 49 714.6 0 49 141 33 714.6 0 33 142 59 714.6 0 59 143 39 714.6 0 39 144 50 714.6 0 50 145 51 714.6 0 51 146 237 714.6 0 237 147 125 714.6 0 125 148 369 714.6 0 369 149 40 714.6 0 40 150 71 714.6 0 71 151 32 714.6 0 32 152 23 714.6 0 23 153 39 714.6 0 39 154 38 714.6 0 38 155 79 714.6 0 79 156 78 714.6 0 78 157 37 714.6 0 37 158 41 714.6 0 41 159 36 714.6 0 36 160 41 714.6 0 41 161 42 714.6 0 42 162 25 714.6 0 25 163 25 714.6 0 25 164 47 714.6 0 47 165 53 714.6 0 53 166 54 714.6 0 54 167 52 714.6 0 52 168 54 714.6 0 54 169 29 714.6 0 29 170 31 714.6 0 31 171 41 714.6 0 41 172 31 714.6 0 31 173 23 714.6 0 23 174 27 714.6 0 27 175 32 714.6 0 32 176 20 714.6 0 20 177 30 714.6 0 30 178 32 714.6 0 32 179 33 714.6 0 33 180 33 714.6 0 33 181 45 714.6 0 45 182 25 714.6 0 25 183 28 714.6 0 28 184 64 714.6 0 64 185 68 714.6 0 68 186 75 714.6 0 75 187 42 714.6 0 42 188 18 714.6 0 18 189 45 714.6 0 45 190 16 714.6 0 16 191 22 714.6 0 22 192 31 714.6 0 31 193 23 714.6 0 23 194 30 714.6 0 30 195 33 714.6 0 33 196 52 714.6 0 52 197 49 714.6 0 49 198 33 714.6 0 33 199 31 714.6 0 31 200 16 714.6 0 16 201 26 714.6 0 26 202 24 714.6 0 24 203 24 714.6 0 24 204 67 714.6 0 67 205 50 714.6 0 50 206 30 714.6 0 30 207 44 714.6 0 44 208 25 714.6 0 25 209 38 714.6 0 38 210 41 714.6 0 41 211 28 714.6 0 28 212 31 714.6 0 31 213 38 714.6 0 38 214 44 714.6 0 44 215 39 714.6 0 39 216 66 714.6 0 66 217 64 714.6 0 64 218 43 714.6 0 43 219 58 714.6 0 58 220 40 714.6 0 40 221 43 714.6 0 43 222 26 714.6 0 26 223 43 714.6 0 43 224 49 714.6 0 49 225 61 714.6 0 61 226 30 714.6 0 30 227 38 714.6 0 38 228 46 714.6 0 46 229 27 714.6 0 27 230 30 714.6 0 30 231 48 714.6 0 48 232 56 714.6 0 56 233 85 714.6 0 85 234 127 714.6 0 127 235 271 714.6 0 271 236 4107 714.6 0 4107 237 1923 714.6 0 1923 238 786 714.6 0 786 239 41 714.6 0 41 240 34 714.6 0 34 241 38 714.6 0 38 242 26 714.6 0 26 243 28 714.6 0 28 244 39 714.6 0 39 245 39 714.6 0 39 246 69 714.6 0 69 247 26 714.6 0 26 248 57 714.6 0 57 249 34 714.6 0 34 250 26 714.6 0 26 251 17 714.6 0 17 252 26 714.6 0 26 253 32 714.6 0 32 254 53 714.6 0 53 255 47 714.6 0 47 256 40 714.6 0 40 257 33 714.6 0 33 258 39 714.6 0 39 259 30 714.6 0 30 260 24 714.6 0 24 261 28 714.6 0 28 262 21 714.6 0 21 263 29 714.6 0 29 264 41 714.6 0 41 265 42 714.6 0 42 266 45 714.6 0 45 267 34 714.6 0 34 268 32 714.6 0 32 269 23 714.6 0 23 270 22 714.6 0 22 271 23 714.6 0 23 272 72 714.6 0 72 273 41 714.6 0 41 274 28 714.6 0 28 275 28 714.6 0 28 276 28 714.6 0 28 277 52 714.6 0 52 278 41 714.6 0 41 279 43 714.6 0 43 280 60 714.6 0 60 281 80 714.6 0 80 282 138 714.6 0 138 283 56 714.6 0 56 284 44 714.6 0 44 285 63 714.6 0 63 286 33 714.6 0 33 287 28 714.6 0 28 288 33 714.6 0 33 289 19 714.6 0 19 290 20 714.6 0 20 291 18 714.6 0 18 292 25 714.6 0 25 293 32 714.6 0 32 294 14 714.6 0 14 295 24 714.6 0 24 296 37 714.6 0 37 297 38 714.6 0 38 298 40 714.6 0 40 299 29 714.6 0 29 300 43 714.6 0 43 301 48 714.6 0 48 Finished in 1.88 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 43,988 Reads with adapters: 1,673 (3.8%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 1,662 (3.8%) Total basepairs processed: 11,199,806 bp Total written (filtered): 454,083 bp (4.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1673 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 8 0.0 3 0 0 7 1 16 21 0.0 3 0 20 1 17 1565 0.0 3 1540 22 2 1 18 8 0.0 3 3 1 4 19 34 0.0 3 0 0 2 32 20 3 0.0 3 0 0 0 3 21 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 39 6 0.0 3 0 0 0 6 42 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 0 3 114 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 231 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 1,662 Reads with adapters: 185 (11.1%) Reads written (passing filters): 1,662 (100.0%) Total basepairs processed: 454,083 bp Total written (filtered): 453,528 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 185 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.2% C: 91.4% G: 3.8% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "C" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 185 26.0 0 185 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.