This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.39 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 102,017 (3.5%) Reads that were too short: 36,576 (1.2%) Reads written (passing filters): 65,441 (2.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 16,413,934 bp (1.9%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 102017 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1660 714.6 0 1660 7 673 714.6 0 673 8 24330 714.6 0 24330 9 81 714.6 0 81 10 86 714.6 0 86 11 64 714.6 0 64 12 62 714.6 0 62 13 55 714.6 0 55 14 66 714.6 0 66 15 50 714.6 0 50 16 62 714.6 0 62 17 36 714.6 0 36 18 39 714.6 0 39 19 96 714.6 0 96 20 418 714.6 0 418 21 70 714.6 0 70 22 33 714.6 0 33 23 47 714.6 0 47 24 47 714.6 0 47 25 46 714.6 0 46 26 48 714.6 0 48 27 47 714.6 0 47 28 51 714.6 0 51 29 63 714.6 0 63 30 43 714.6 0 43 31 50 714.6 0 50 32 37 714.6 0 37 33 43 714.6 0 43 34 47 714.6 0 47 35 48 714.6 0 48 36 56 714.6 0 56 37 49 714.6 0 49 38 52 714.6 0 52 39 36 714.6 0 36 40 37 714.6 0 37 41 45 714.6 0 45 42 42 714.6 0 42 43 34 714.6 0 34 44 47 714.6 0 47 45 28 714.6 0 28 46 42 714.6 0 42 47 38 714.6 0 38 48 48 714.6 0 48 49 36 714.6 0 36 50 49 714.6 0 49 51 48 714.6 0 48 52 36 714.6 0 36 53 52 714.6 0 52 54 33 714.6 0 33 55 59 714.6 0 59 56 52 714.6 0 52 57 35 714.6 0 35 58 46 714.6 0 46 59 64 714.6 0 64 60 86 714.6 0 86 61 160 714.6 0 160 62 329 714.6 0 329 63 101 714.6 0 101 64 114 714.6 0 114 65 255 714.6 0 255 66 109 714.6 0 109 67 96 714.6 0 96 68 164 714.6 0 164 69 147 714.6 0 147 70 178 714.6 0 178 71 199 714.6 0 199 72 219 714.6 0 219 73 254 714.6 0 254 74 464 714.6 0 464 75 8979 714.6 0 8979 76 10255 714.6 0 10255 77 9755 714.6 0 9755 78 72 714.6 0 72 79 18 714.6 0 18 80 30 714.6 0 30 81 26 714.6 0 26 82 20 714.6 0 20 83 26 714.6 0 26 84 41 714.6 0 41 85 20 714.6 0 20 86 24 714.6 0 24 87 25 714.6 0 25 88 56 714.6 0 56 89 30 714.6 0 30 90 32 714.6 0 32 91 36 714.6 0 36 92 31 714.6 0 31 93 17 714.6 0 17 94 22 714.6 0 22 95 35 714.6 0 35 96 22 714.6 0 22 97 24 714.6 0 24 98 24 714.6 0 24 99 17 714.6 0 17 100 10 714.6 0 10 101 19 714.6 0 19 102 20 714.6 0 20 103 10 714.6 0 10 104 10 714.6 0 10 105 18 714.6 0 18 106 19 714.6 0 19 107 10 714.6 0 10 108 15 714.6 0 15 109 19 714.6 0 19 110 10 714.6 0 10 111 9 714.6 0 9 112 15 714.6 0 15 113 12 714.6 0 12 114 17 714.6 0 17 115 23 714.6 0 23 116 28 714.6 0 28 117 20 714.6 0 20 118 20 714.6 0 20 119 20 714.6 0 20 120 24 714.6 0 24 121 34 714.6 0 34 122 22 714.6 0 22 123 20 714.6 0 20 124 31 714.6 0 31 125 35 714.6 0 35 126 45 714.6 0 45 127 203 714.6 0 203 128 26 714.6 0 26 129 54 714.6 0 54 130 21 714.6 0 21 131 17 714.6 0 17 132 20 714.6 0 20 133 32 714.6 0 32 134 82 714.6 0 82 135 63 714.6 0 63 136 54 714.6 0 54 137 117 714.6 0 117 138 35 714.6 0 35 139 18 714.6 0 18 140 10 714.6 0 10 141 22 714.6 0 22 142 21 714.6 0 21 143 20 714.6 0 20 144 14 714.6 0 14 145 18 714.6 0 18 146 14 714.6 0 14 147 24 714.6 0 24 148 13 714.6 0 13 149 18 714.6 0 18 150 25 714.6 0 25 151 12 714.6 0 12 152 16 714.6 0 16 153 11 714.6 0 11 154 23 714.6 0 23 155 21 714.6 0 21 156 25 714.6 0 25 157 28 714.6 0 28 158 25 714.6 0 25 159 25 714.6 0 25 160 15 714.6 0 15 161 38 714.6 0 38 162 37 714.6 0 37 163 43 714.6 0 43 164 37 714.6 0 37 165 20 714.6 0 20 166 41 714.6 0 41 167 44 714.6 0 44 168 50 714.6 0 50 169 38 714.6 0 38 170 50 714.6 0 50 171 33 714.6 0 33 172 24 714.6 0 24 173 19 714.6 0 19 174 24 714.6 0 24 175 27 714.6 0 27 176 23 714.6 0 23 177 16 714.6 0 16 178 18 714.6 0 18 179 26 714.6 0 26 180 29 714.6 0 29 181 35 714.6 0 35 182 52 714.6 0 52 183 70 714.6 0 70 184 67 714.6 0 67 185 23 714.6 0 23 186 53 714.6 0 53 187 35 714.6 0 35 188 68 714.6 0 68 189 34 714.6 0 34 190 19 714.6 0 19 191 33 714.6 0 33 192 44 714.6 0 44 193 57 714.6 0 57 194 95 714.6 0 95 195 62 714.6 0 62 196 41 714.6 0 41 197 88 714.6 0 88 198 61 714.6 0 61 199 58 714.6 0 58 200 29 714.6 0 29 201 29 714.6 0 29 202 25 714.6 0 25 203 50 714.6 0 50 204 81 714.6 0 81 205 57 714.6 0 57 206 47 714.6 0 47 207 26 714.6 0 26 208 30 714.6 0 30 209 27 714.6 0 27 210 45 714.6 0 45 211 41 714.6 0 41 212 35 714.6 0 35 213 65 714.6 0 65 214 74 714.6 0 74 215 57 714.6 0 57 216 55 714.6 0 55 217 91 714.6 0 91 218 70 714.6 0 70 219 86 714.6 0 86 220 48 714.6 0 48 221 76 714.6 0 76 222 53 714.6 0 53 223 200 714.6 0 200 224 247 714.6 0 247 225 452 714.6 0 452 226 68 714.6 0 68 227 56 714.6 0 56 228 55 714.6 0 55 229 30 714.6 0 30 230 34 714.6 0 34 231 49 714.6 0 49 232 73 714.6 0 73 233 109 714.6 0 109 234 193 714.6 0 193 235 377 714.6 0 377 236 340 714.6 0 340 237 450 714.6 0 450 238 6067 714.6 0 6067 239 5600 714.6 0 5600 240 1887 714.6 0 1887 241 151 714.6 0 151 242 139 714.6 0 139 243 160 714.6 0 160 244 164 714.6 0 164 245 188 714.6 0 188 246 242 714.6 0 242 247 340 714.6 0 340 248 3106 714.6 0 3106 249 4745 714.6 0 4745 250 6441 714.6 0 6441 251 1221 714.6 0 1221 252 36 714.6 0 36 253 47 714.6 0 47 254 38 714.6 0 38 255 53 714.6 0 53 256 38 714.6 0 38 257 28 714.6 0 28 258 13 714.6 0 13 259 14 714.6 0 14 260 40 714.6 0 40 261 88 714.6 0 88 262 22 714.6 0 22 263 71 714.6 0 71 264 30 714.6 0 30 265 21 714.6 0 21 266 42 714.6 0 42 267 25 714.6 0 25 268 19 714.6 0 19 269 28 714.6 0 28 270 13 714.6 0 13 271 26 714.6 0 26 272 17 714.6 0 17 273 13 714.6 0 13 274 18 714.6 0 18 275 13 714.6 0 13 276 12 714.6 0 12 277 19 714.6 0 19 278 21 714.6 0 21 279 50 714.6 0 50 280 126 714.6 0 126 281 36 714.6 0 36 282 98 714.6 0 98 283 310 714.6 0 310 284 287 714.6 0 287 285 34 714.6 0 34 286 28 714.6 0 28 287 29 714.6 0 29 288 19 714.6 0 19 289 13 714.6 0 13 290 12 714.6 0 12 291 9 714.6 0 9 292 24 714.6 0 24 293 23 714.6 0 23 294 43 714.6 0 43 295 57 714.6 0 57 296 54 714.6 0 54 297 50 714.6 0 50 298 22 714.6 0 22 299 18 714.6 0 18 300 39 714.6 0 39 301 67 714.6 0 67 Finished in 3.17 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 65,441 Reads with adapters: 26,401 (40.3%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 26,391 (40.3%) Total basepairs processed: 16,413,934 bp Total written (filtered): 7,205,952 bp (43.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26401 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 15 0.0 3 0 0 12 2 1 19 396 0.0 3 0 362 19 13 2 20 24703 0.0 4 23878 692 102 27 4 21 82 0.0 4 12 67 2 0 1 22 1148 0.0 4 1101 38 7 2 23 32 0.0 4 2 3 0 20 7 24 4 0.0 4 1 0 0 0 3 25 5 0.0 4 2 0 1 1 1 37 1 0.0 4 1 49 1 0.0 4 0 0 0 0 1 72 2 0.0 4 0 0 0 0 2 89 1 0.0 4 1 95 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 166 2 0.0 4 0 0 0 1 1 170 2 0.0 4 0 0 0 0 2 223 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.92 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 26,391 Reads with adapters: 2,723 (10.3%) Reads written (passing filters): 26,391 (100.0%) Total basepairs processed: 7,205,952 bp Total written (filtered): 7,080,193 bp (98.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2723 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.9% C: 2.8% G: 78.9% T: 5.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 412.4 0 3 4 4 103.1 0 4 6 1 6.4 0 1 9 16 0.1 0 1 15 10 75 0.0 1 6 69 11 2211 0.0 1 11 2200 12 9 0.0 1 0 9 13 1 0.0 1 0 1 14 1 0.0 1 0 1 209 3 0.0 1 3 224 106 0.0 1 93 13 225 4 0.0 1 4 226 1 0.0 1 1 232 1 0.0 1 1 258 8 0.0 1 8 259 175 0.0 1 164 11 260 17 0.0 1 15 2 261 3 0.0 1 3 262 74 0.0 1 64 10 263 3 0.0 1 3 264 3 0.0 1 3 272 4 0.0 1 3 1