This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.53 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 447,218 (15.3%) Reads that were too short: 200,795 (6.9%) Reads written (passing filters): 246,423 (8.4%) Total basepairs processed: 881,011,047 bp Total written (filtered): 45,916,602 bp (5.2%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 447218 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1111 714.6 0 1111 7 30872 714.6 0 30872 8 448 714.6 0 448 9 21808 714.6 0 21808 10 52 714.6 0 52 11 14 714.6 0 14 12 16 714.6 0 16 13 10 714.6 0 10 14 12 714.6 0 12 15 12 714.6 0 12 16 8 714.6 0 8 17 14 714.6 0 14 18 14 714.6 0 14 19 10 714.6 0 10 20 16 714.6 0 16 21 13 714.6 0 13 22 9 714.6 0 9 23 8 714.6 0 8 24 12 714.6 0 12 25 7 714.6 0 7 26 7 714.6 0 7 27 12 714.6 0 12 28 14 714.6 0 14 29 14 714.6 0 14 30 14 714.6 0 14 31 8 714.6 0 8 32 11 714.6 0 11 33 12 714.6 0 12 34 14 714.6 0 14 35 22 714.6 0 22 36 9 714.6 0 9 37 13 714.6 0 13 38 15 714.6 0 15 39 15 714.6 0 15 40 44 714.6 0 44 41 177 714.6 0 177 42 42 714.6 0 42 43 8 714.6 0 8 44 8 714.6 0 8 45 8 714.6 0 8 46 10 714.6 0 10 47 17 714.6 0 17 48 18 714.6 0 18 49 4 714.6 0 4 50 4 714.6 0 4 51 4 714.6 0 4 52 4 714.6 0 4 53 6 714.6 0 6 54 13 714.6 0 13 55 15 714.6 0 15 56 9 714.6 0 9 57 8 714.6 0 8 58 6 714.6 0 6 59 16 714.6 0 16 60 10 714.6 0 10 61 7 714.6 0 7 62 10 714.6 0 10 63 14 714.6 0 14 64 15 714.6 0 15 65 38 714.6 0 38 66 39 714.6 0 39 67 42 714.6 0 42 68 4 714.6 0 4 69 17 714.6 0 17 70 11 714.6 0 11 71 27 714.6 0 27 72 21 714.6 0 21 73 8 714.6 0 8 74 7 714.6 0 7 75 5 714.6 0 5 76 13 714.6 0 13 77 15 714.6 0 15 78 60 714.6 0 60 79 181 714.6 0 181 80 214 714.6 0 214 81 1237 714.6 0 1237 82 1027 714.6 0 1027 83 1155 714.6 0 1155 84 669 714.6 0 669 85 874 714.6 0 874 86 1937 714.6 0 1937 87 1627 714.6 0 1627 88 1489 714.6 0 1489 89 1119 714.6 0 1119 90 393 714.6 0 393 91 726 714.6 0 726 92 646 714.6 0 646 93 1055 714.6 0 1055 94 1050 714.6 0 1050 95 564 714.6 0 564 96 630 714.6 0 630 97 1644 714.6 0 1644 98 1486 714.6 0 1486 99 1187 714.6 0 1187 100 673 714.6 0 673 101 707 714.6 0 707 102 1894 714.6 0 1894 103 1443 714.6 0 1443 104 872 714.6 0 872 105 1203 714.6 0 1203 106 1057 714.6 0 1057 107 1552 714.6 0 1552 108 1026 714.6 0 1026 109 1612 714.6 0 1612 110 1718 714.6 0 1718 111 1353 714.6 0 1353 112 1155 714.6 0 1155 113 1629 714.6 0 1629 114 1319 714.6 0 1319 115 1115 714.6 0 1115 116 927 714.6 0 927 117 940 714.6 0 940 118 2075 714.6 0 2075 119 2020 714.6 0 2020 120 1304 714.6 0 1304 121 766 714.6 0 766 122 1288 714.6 0 1288 123 1471 714.6 0 1471 124 1574 714.6 0 1574 125 2008 714.6 0 2008 126 2224 714.6 0 2224 127 1940 714.6 0 1940 128 1449 714.6 0 1449 129 1172 714.6 0 1172 130 1191 714.6 0 1191 131 1192 714.6 0 1192 132 1386 714.6 0 1386 133 2901 714.6 0 2901 134 2178 714.6 0 2178 135 1341 714.6 0 1341 136 1215 714.6 0 1215 137 1331 714.6 0 1331 138 2631 714.6 0 2631 139 3102 714.6 0 3102 140 2648 714.6 0 2648 141 1418 714.6 0 1418 142 1001 714.6 0 1001 143 1494 714.6 0 1494 144 2603 714.6 0 2603 145 2268 714.6 0 2268 146 2106 714.6 0 2106 147 1794 714.6 0 1794 148 1243 714.6 0 1243 149 2028 714.6 0 2028 150 2103 714.6 0 2103 151 2703 714.6 0 2703 152 2842 714.6 0 2842 153 1883 714.6 0 1883 154 2129 714.6 0 2129 155 1567 714.6 0 1567 156 2052 714.6 0 2052 157 3018 714.6 0 3018 158 1777 714.6 0 1777 159 2267 714.6 0 2267 160 1807 714.6 0 1807 161 1863 714.6 0 1863 162 1332 714.6 0 1332 163 1442 714.6 0 1442 164 2609 714.6 0 2609 165 2045 714.6 0 2045 166 1671 714.6 0 1671 167 1236 714.6 0 1236 168 1212 714.6 0 1212 169 1116 714.6 0 1116 170 1273 714.6 0 1273 171 1630 714.6 0 1630 172 2700 714.6 0 2700 173 1960 714.6 0 1960 174 1262 714.6 0 1262 175 904 714.6 0 904 176 1091 714.6 0 1091 177 1361 714.6 0 1361 178 1298 714.6 0 1298 179 2275 714.6 0 2275 180 2528 714.6 0 2528 181 2359 714.6 0 2359 182 1436 714.6 0 1436 183 1218 714.6 0 1218 184 1596 714.6 0 1596 185 2095 714.6 0 2095 186 1670 714.6 0 1670 187 1495 714.6 0 1495 188 1660 714.6 0 1660 189 1988 714.6 0 1988 190 2266 714.6 0 2266 191 1627 714.6 0 1627 192 1368 714.6 0 1368 193 1269 714.6 0 1269 194 1299 714.6 0 1299 195 1181 714.6 0 1181 196 1532 714.6 0 1532 197 1687 714.6 0 1687 198 992 714.6 0 992 199 2065 714.6 0 2065 200 1779 714.6 0 1779 201 1988 714.6 0 1988 202 2048 714.6 0 2048 203 1203 714.6 0 1203 204 1283 714.6 0 1283 205 1782 714.6 0 1782 206 2174 714.6 0 2174 207 2207 714.6 0 2207 208 1808 714.6 0 1808 209 1600 714.6 0 1600 210 2062 714.6 0 2062 211 3685 714.6 0 3685 212 6480 714.6 0 6480 213 4621 714.6 0 4621 214 3692 714.6 0 3692 215 1761 714.6 0 1761 216 1483 714.6 0 1483 217 1543 714.6 0 1543 218 1723 714.6 0 1723 219 2121 714.6 0 2121 220 2556 714.6 0 2556 221 1724 714.6 0 1724 222 1628 714.6 0 1628 223 1950 714.6 0 1950 224 2296 714.6 0 2296 225 2025 714.6 0 2025 226 1717 714.6 0 1717 227 1476 714.6 0 1476 228 1411 714.6 0 1411 229 1729 714.6 0 1729 230 1545 714.6 0 1545 231 1680 714.6 0 1680 232 1604 714.6 0 1604 233 1812 714.6 0 1812 234 2017 714.6 0 2017 235 1639 714.6 0 1639 236 1608 714.6 0 1608 237 1603 714.6 0 1603 238 2147 714.6 0 2147 239 2012 714.6 0 2012 240 2207 714.6 0 2207 241 2410 714.6 0 2410 242 2263 714.6 0 2263 243 1981 714.6 0 1981 244 1509 714.6 0 1509 245 2156 714.6 0 2156 246 1534 714.6 0 1534 247 1516 714.6 0 1516 248 910 714.6 0 910 249 1961 714.6 0 1961 250 2210 714.6 0 2210 251 2699 714.6 0 2699 252 2287 714.6 0 2287 253 1142 714.6 0 1142 254 1546 714.6 0 1546 255 1729 714.6 0 1729 256 1765 714.6 0 1765 257 1664 714.6 0 1664 258 1699 714.6 0 1699 259 2001 714.6 0 2001 260 1914 714.6 0 1914 261 2008 714.6 0 2008 262 1952 714.6 0 1952 263 1439 714.6 0 1439 264 1533 714.6 0 1533 265 1726 714.6 0 1726 266 1514 714.6 0 1514 267 2163 714.6 0 2163 268 1428 714.6 0 1428 269 1725 714.6 0 1725 270 2890 714.6 0 2890 271 2629 714.6 0 2629 272 2429 714.6 0 2429 273 1761 714.6 0 1761 274 2107 714.6 0 2107 275 1862 714.6 0 1862 276 1674 714.6 0 1674 277 2470 714.6 0 2470 278 2512 714.6 0 2512 279 1885 714.6 0 1885 280 1947 714.6 0 1947 281 1273 714.6 0 1273 282 2080 714.6 0 2080 283 2307 714.6 0 2307 284 1421 714.6 0 1421 285 1644 714.6 0 1644 286 1728 714.6 0 1728 287 1864 714.6 0 1864 288 2036 714.6 0 2036 289 2172 714.6 0 2172 290 1910 714.6 0 1910 291 1695 714.6 0 1695 292 1879 714.6 0 1879 293 2814 714.6 0 2814 294 3289 714.6 0 3289 295 2141 714.6 0 2141 296 2331 714.6 0 2331 297 2144 714.6 0 2144 298 2068 714.6 0 2068 299 2433 714.6 0 2433 300 2406 714.6 0 2406 301 948 714.6 0 948 Finished in 8.28 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 246,423 Reads with adapters: 22,459 (9.1%) Reads that were too short: 46 (0.0%) Reads written (passing filters): 22,413 (9.1%) Total basepairs processed: 45,916,602 bp Total written (filtered): 6,159,881 bp (13.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22459 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 8 0.0 3 0 0 8 16 154 0.0 3 0 150 3 1 17 22140 0.0 3 21672 457 11 18 46 0.0 3 2 42 2 19 9 0.0 3 0 0 5 4 20 15 0.0 3 0 0 0 15 21 1 0.0 3 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 1 25 1 0.0 3 1 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 29 2 0.0 3 1 0 0 1 30 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 34 1 0.0 3 1 35 1 0.0 3 0 0 0 1 37 2 0.0 3 1 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 0 3 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 80 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 102 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 22,413 Reads with adapters: 1,025 (4.6%) Reads written (passing filters): 22,413 (100.0%) Total basepairs processed: 6,159,881 bp Total written (filtered): 6,156,803 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1025 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.8% C: 14.8% G: 54.8% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1023 350.2 0 1023 4 1 87.6 0 1 5 1 21.9 0 1