This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 80.13 s (27 us/read; 2.19 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 824,757 (28.2%) Reads that were too short: 288,612 (9.9%) Reads written (passing filters): 536,145 (18.3%) Total basepairs processed: 881,011,047 bp Total written (filtered): 94,559,481 bp (10.7%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 824757 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 937 714.6 0 937 7 37030 714.6 0 37030 8 89 714.6 0 89 9 66 714.6 0 66 10 31 714.6 0 31 11 47 714.6 0 47 12 27 714.6 0 27 13 22 714.6 0 22 14 12 714.6 0 12 15 25 714.6 0 25 16 17 714.6 0 17 17 16 714.6 0 16 18 15 714.6 0 15 19 13 714.6 0 13 20 14 714.6 0 14 21 16 714.6 0 16 22 9 714.6 0 9 23 22 714.6 0 22 24 48 714.6 0 48 25 29 714.6 0 29 26 20 714.6 0 20 27 31 714.6 0 31 28 26 714.6 0 26 29 44 714.6 0 44 30 33 714.6 0 33 31 18 714.6 0 18 32 20 714.6 0 20 33 19 714.6 0 19 34 17 714.6 0 17 35 12 714.6 0 12 36 28 714.6 0 28 37 21 714.6 0 21 38 19 714.6 0 19 39 25 714.6 0 25 40 17 714.6 0 17 41 12 714.6 0 12 42 17 714.6 0 17 43 13 714.6 0 13 44 18 714.6 0 18 45 12 714.6 0 12 46 17 714.6 0 17 47 24 714.6 0 24 48 30 714.6 0 30 49 28 714.6 0 28 50 22 714.6 0 22 51 20 714.6 0 20 52 17 714.6 0 17 53 15 714.6 0 15 54 29 714.6 0 29 55 24 714.6 0 24 56 29 714.6 0 29 57 140 714.6 0 140 58 20 714.6 0 20 59 26 714.6 0 26 60 23 714.6 0 23 61 23 714.6 0 23 62 29 714.6 0 29 63 79 714.6 0 79 64 97 714.6 0 97 65 81 714.6 0 81 66 49 714.6 0 49 67 58 714.6 0 58 68 40 714.6 0 40 69 112 714.6 0 112 70 436 714.6 0 436 71 2433 714.6 0 2433 72 4277 714.6 0 4277 73 5271 714.6 0 5271 74 5797 714.6 0 5797 75 2522 714.6 0 2522 76 2539 714.6 0 2539 77 3664 714.6 0 3664 78 4255 714.6 0 4255 79 5314 714.6 0 5314 80 5032 714.6 0 5032 81 3959 714.6 0 3959 82 5206 714.6 0 5206 83 6727 714.6 0 6727 84 4655 714.6 0 4655 85 6310 714.6 0 6310 86 7680 714.6 0 7680 87 7621 714.6 0 7621 88 4749 714.6 0 4749 89 5249 714.6 0 5249 90 2319 714.6 0 2319 91 3415 714.6 0 3415 92 6512 714.6 0 6512 93 3164 714.6 0 3164 94 3579 714.6 0 3579 95 3286 714.6 0 3286 96 2165 714.6 0 2165 97 1314 714.6 0 1314 98 1706 714.6 0 1706 99 1995 714.6 0 1995 100 4136 714.6 0 4136 101 4333 714.6 0 4333 102 3736 714.6 0 3736 103 4573 714.6 0 4573 104 3436 714.6 0 3436 105 2716 714.6 0 2716 106 1566 714.6 0 1566 107 1612 714.6 0 1612 108 3178 714.6 0 3178 109 4028 714.6 0 4028 110 4882 714.6 0 4882 111 4152 714.6 0 4152 112 4267 714.6 0 4267 113 3454 714.6 0 3454 114 3101 714.6 0 3101 115 2859 714.6 0 2859 116 4489 714.6 0 4489 117 4589 714.6 0 4589 118 5238 714.6 0 5238 119 4795 714.6 0 4795 120 4753 714.6 0 4753 121 4575 714.6 0 4575 122 4517 714.6 0 4517 123 4019 714.6 0 4019 124 3810 714.6 0 3810 125 3711 714.6 0 3711 126 3268 714.6 0 3268 127 2890 714.6 0 2890 128 3004 714.6 0 3004 129 3555 714.6 0 3555 130 3327 714.6 0 3327 131 4635 714.6 0 4635 132 3670 714.6 0 3670 133 3004 714.6 0 3004 134 3173 714.6 0 3173 135 3035 714.6 0 3035 136 3078 714.6 0 3078 137 3433 714.6 0 3433 138 3851 714.6 0 3851 139 3614 714.6 0 3614 140 3241 714.6 0 3241 141 3330 714.6 0 3330 142 4144 714.6 0 4144 143 3733 714.6 0 3733 144 3466 714.6 0 3466 145 3986 714.6 0 3986 146 3732 714.6 0 3732 147 3585 714.6 0 3585 148 3552 714.6 0 3552 149 2895 714.6 0 2895 150 3521 714.6 0 3521 151 3286 714.6 0 3286 152 4251 714.6 0 4251 153 3884 714.6 0 3884 154 2928 714.6 0 2928 155 3263 714.6 0 3263 156 2855 714.6 0 2855 157 3151 714.6 0 3151 158 3598 714.6 0 3598 159 2742 714.6 0 2742 160 3055 714.6 0 3055 161 2700 714.6 0 2700 162 3088 714.6 0 3088 163 2735 714.6 0 2735 164 2403 714.6 0 2403 165 2293 714.6 0 2293 166 3799 714.6 0 3799 167 4552 714.6 0 4552 168 4033 714.6 0 4033 169 3169 714.6 0 3169 170 4077 714.6 0 4077 171 4779 714.6 0 4779 172 4012 714.6 0 4012 173 3009 714.6 0 3009 174 2635 714.6 0 2635 175 3460 714.6 0 3460 176 3009 714.6 0 3009 177 3429 714.6 0 3429 178 3529 714.6 0 3529 179 3004 714.6 0 3004 180 3774 714.6 0 3774 181 4071 714.6 0 4071 182 19374 714.6 0 19374 183 4766 714.6 0 4766 184 3751 714.6 0 3751 185 3861 714.6 0 3861 186 2697 714.6 0 2697 187 3056 714.6 0 3056 188 3217 714.6 0 3217 189 2934 714.6 0 2934 190 3103 714.6 0 3103 191 3199 714.6 0 3199 192 2900 714.6 0 2900 193 3037 714.6 0 3037 194 2250 714.6 0 2250 195 2903 714.6 0 2903 196 2345 714.6 0 2345 197 3742 714.6 0 3742 198 4839 714.6 0 4839 199 3421 714.6 0 3421 200 3558 714.6 0 3558 201 2282 714.6 0 2282 202 2623 714.6 0 2623 203 3146 714.6 0 3146 204 3105 714.6 0 3105 205 3087 714.6 0 3087 206 3038 714.6 0 3038 207 2414 714.6 0 2414 208 2265 714.6 0 2265 209 2168 714.6 0 2168 210 2413 714.6 0 2413 211 2771 714.6 0 2771 212 2663 714.6 0 2663 213 4331 714.6 0 4331 214 3654 714.6 0 3654 215 2975 714.6 0 2975 216 2401 714.6 0 2401 217 2343 714.6 0 2343 218 3158 714.6 0 3158 219 3438 714.6 0 3438 220 2546 714.6 0 2546 221 2122 714.6 0 2122 222 2293 714.6 0 2293 223 2857 714.6 0 2857 224 2495 714.6 0 2495 225 2184 714.6 0 2184 226 2173 714.6 0 2173 227 2209 714.6 0 2209 228 2805 714.6 0 2805 229 2758 714.6 0 2758 230 2183 714.6 0 2183 231 2243 714.6 0 2243 232 2321 714.6 0 2321 233 2699 714.6 0 2699 234 2638 714.6 0 2638 235 2475 714.6 0 2475 236 1754 714.6 0 1754 237 2290 714.6 0 2290 238 2425 714.6 0 2425 239 3631 714.6 0 3631 240 2917 714.6 0 2917 241 2548 714.6 0 2548 242 2962 714.6 0 2962 243 2535 714.6 0 2535 244 2469 714.6 0 2469 245 2106 714.6 0 2106 246 2799 714.6 0 2799 247 2598 714.6 0 2598 248 2540 714.6 0 2540 249 2352 714.6 0 2352 250 3074 714.6 0 3074 251 2936 714.6 0 2936 252 3716 714.6 0 3716 253 2780 714.6 0 2780 254 2293 714.6 0 2293 255 1695 714.6 0 1695 256 1900 714.6 0 1900 257 2009 714.6 0 2009 258 1790 714.6 0 1790 259 1797 714.6 0 1797 260 1957 714.6 0 1957 261 3196 714.6 0 3196 262 4488 714.6 0 4488 263 4363 714.6 0 4363 264 3703 714.6 0 3703 265 2515 714.6 0 2515 266 2407 714.6 0 2407 267 7148 714.6 0 7148 268 6980 714.6 0 6980 269 7761 714.6 0 7761 270 2955 714.6 0 2955 271 2185 714.6 0 2185 272 3230 714.6 0 3230 273 2868 714.6 0 2868 274 2085 714.6 0 2085 275 1954 714.6 0 1954 276 1798 714.6 0 1798 277 2953 714.6 0 2953 278 3803 714.6 0 3803 279 3039 714.6 0 3039 280 2409 714.6 0 2409 281 2912 714.6 0 2912 282 3411 714.6 0 3411 283 2347 714.6 0 2347 284 2537 714.6 0 2537 285 2308 714.6 0 2308 286 2324 714.6 0 2324 287 2359 714.6 0 2359 288 1835 714.6 0 1835 289 2379 714.6 0 2379 290 3516 714.6 0 3516 291 3360 714.6 0 3360 292 3488 714.6 0 3488 293 2682 714.6 0 2682 294 2352 714.6 0 2352 295 2465 714.6 0 2465 296 2782 714.6 0 2782 297 3363 714.6 0 3363 298 3512 714.6 0 3512 299 4837 714.6 0 4837 300 4843 714.6 0 4843 301 4293 714.6 0 4293 Finished in 18.76 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 536,145 Reads with adapters: 38,237 (7.1%) Reads that were too short: 220 (0.0%) Reads written (passing filters): 38,017 (7.1%) Total basepairs processed: 94,559,481 bp Total written (filtered): 10,402,288 bp (11.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 38237 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 18 0.0 3 0 0 14 2 2 19 328 0.0 3 0 308 12 4 4 20 37408 0.0 4 36569 724 83 28 4 21 118 0.0 4 19 96 0 2 1 22 19 0.0 4 0 1 9 0 9 23 19 0.0 4 0 1 1 13 4 24 6 0.0 4 0 0 0 0 6 25 6 0.0 4 0 0 0 0 6 26 2 0.0 4 0 0 0 0 2 27 4 0.0 4 1 0 0 0 3 28 5 0.0 4 1 0 0 1 3 29 1 0.0 4 0 0 0 0 1 30 2 0.0 4 0 0 0 0 2 32 1 0.0 4 0 0 0 0 1 33 2 0.0 4 1 0 0 0 1 34 2 0.0 4 0 0 0 0 2 37 3 0.0 4 0 0 0 0 3 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 41 4 0.0 4 0 0 0 0 4 42 2 0.0 4 0 0 0 0 2 43 3 0.0 4 0 0 0 0 3 44 2 0.0 4 0 0 0 0 2 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 47 2 0.0 4 0 0 0 0 2 48 4 0.0 4 0 0 0 0 4 49 1 0.0 4 0 0 0 0 1 51 3 0.0 4 0 0 0 0 3 52 3 0.0 4 0 0 0 0 3 55 1 0.0 4 0 0 0 0 1 56 4 0.0 4 0 0 0 0 4 57 3 0.0 4 0 0 0 0 3 58 1 0.0 4 0 0 0 0 1 59 4 0.0 4 0 0 0 0 4 60 2 0.0 4 0 0 0 0 2 61 3 0.0 4 0 0 0 0 3 62 2 0.0 4 0 0 0 0 2 63 3 0.0 4 0 0 0 0 3 64 3 0.0 4 0 0 0 0 3 65 6 0.0 4 0 0 0 0 6 66 4 0.0 4 0 0 0 0 4 67 3 0.0 4 0 0 0 0 3 68 3 0.0 4 0 0 0 0 3 69 3 0.0 4 0 0 0 0 3 70 4 0.0 4 0 0 0 0 4 71 5 0.0 4 0 0 0 0 5 72 2 0.0 4 0 0 0 0 2 73 2 0.0 4 0 0 0 0 2 74 4 0.0 4 0 0 0 0 4 75 3 0.0 4 0 0 0 0 3 76 3 0.0 4 0 0 0 0 3 77 1 0.0 4 0 0 0 0 1 78 2 0.0 4 0 0 0 0 2 79 3 0.0 4 0 0 0 0 3 80 1 0.0 4 0 0 0 0 1 82 4 0.0 4 0 0 0 0 4 83 5 0.0 4 0 0 0 0 5 84 7 0.0 4 0 0 0 0 7 85 3 0.0 4 0 0 0 0 3 86 1 0.0 4 0 0 0 0 1 87 4 0.0 4 0 0 0 0 4 88 1 0.0 4 0 0 0 0 1 89 4 0.0 4 0 0 0 0 4 90 1 0.0 4 0 0 0 0 1 91 3 0.0 4 0 0 0 0 3 92 3 0.0 4 0 0 0 0 3 93 7 0.0 4 0 0 0 0 7 94 2 0.0 4 0 0 0 0 2 95 1 0.0 4 0 0 0 0 1 96 4 0.0 4 0 0 0 0 4 97 3 0.0 4 0 0 0 1 2 98 5 0.0 4 0 0 0 0 5 99 3 0.0 4 0 0 0 0 3 100 4 0.0 4 0 0 0 0 4 101 1 0.0 4 0 0 0 0 1 102 3 0.0 4 0 0 0 0 3 104 1 0.0 4 0 0 0 0 1 105 6 0.0 4 0 0 0 1 5 106 1 0.0 4 0 0 0 0 1 107 4 0.0 4 0 0 0 0 4 108 2 0.0 4 0 0 0 0 2 110 3 0.0 4 0 0 0 1 2 111 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 2 0.0 4 0 0 0 0 2 115 2 0.0 4 0 0 0 0 2 116 3 0.0 4 0 0 0 0 3 118 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 0 2 121 3 0.0 4 0 0 0 0 3 122 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 3 0.0 4 0 0 0 0 3 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 0 2 128 3 0.0 4 0 0 0 0 3 129 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 132 3 0.0 4 0 0 0 0 3 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 4 0.0 4 0 0 0 0 4 136 2 0.0 4 0 0 0 0 2 137 2 0.0 4 0 0 0 0 2 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 2 0.0 4 0 0 0 0 2 142 1 0.0 4 0 0 0 0 1 143 2 0.0 4 0 0 0 0 2 144 1 0.0 4 0 0 0 0 1 145 3 0.0 4 0 0 0 0 3 146 2 0.0 4 0 0 0 0 2 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 2 0.0 4 0 0 0 1 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 155 3 0.0 4 0 0 0 1 2 157 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 163 2 0.0 4 0 0 0 1 1 164 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 168 2 0.0 4 0 0 0 0 2 169 4 0.0 4 0 0 0 0 4 171 3 0.0 4 0 0 0 0 3 172 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 180 2 0.0 4 0 0 0 0 2 181 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.30 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 38,017 Reads with adapters: 675 (1.8%) Reads written (passing filters): 38,017 (100.0%) Total basepairs processed: 10,402,288 bp Total written (filtered): 10,382,800 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 675 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.4% C: 5.0% G: 82.1% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 594.0 0 12 4 2 148.5 0 2 9 1 0.1 0 0 1 10 3 0.0 1 1 2 11 506 0.0 1 5 501 12 98 0.0 1 0 98 13 1 0.0 1 0 1 16 1 0.0 1 0 1 123 1 0.0 1 1 160 1 0.0 1 0 1 180 2 0.0 1 1 1 207 3 0.0 1 3 210 1 0.0 1 1 225 1 0.0 1 1 234 3 0.0 1 3 261 12 0.0 1 11 1 262 1 0.0 1 1 263 25 0.0 1 23 2 264 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.