This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.91 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 210,162 (7.2%) Reads that were too short: 116,905 (4.0%) Reads written (passing filters): 93,257 (3.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 20,613,685 bp (2.3%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 210162 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 618 714.6 0 618 7 713 714.6 0 713 8 36506 714.6 0 36506 9 4705 714.6 0 4705 10 50 714.6 0 50 11 46 714.6 0 46 12 31 714.6 0 31 13 49 714.6 0 49 14 53 714.6 0 53 15 34 714.6 0 34 16 44 714.6 0 44 17 59 714.6 0 59 18 494 714.6 0 494 19 86 714.6 0 86 20 28 714.6 0 28 21 41 714.6 0 41 22 43 714.6 0 43 23 33 714.6 0 33 24 35 714.6 0 35 25 39 714.6 0 39 26 30 714.6 0 30 27 17 714.6 0 17 28 33 714.6 0 33 29 30 714.6 0 30 30 37 714.6 0 37 31 49 714.6 0 49 32 35 714.6 0 35 33 31 714.6 0 31 34 27 714.6 0 27 35 35 714.6 0 35 36 33 714.6 0 33 37 43 714.6 0 43 38 59 714.6 0 59 39 45 714.6 0 45 40 32 714.6 0 32 41 45 714.6 0 45 42 41 714.6 0 41 43 71 714.6 0 71 44 250 714.6 0 250 45 116 714.6 0 116 46 3506 714.6 0 3506 47 402 714.6 0 402 48 55 714.6 0 55 49 32 714.6 0 32 50 33 714.6 0 33 51 31 714.6 0 31 52 56 714.6 0 56 53 45 714.6 0 45 54 136 714.6 0 136 55 34 714.6 0 34 56 112 714.6 0 112 57 33 714.6 0 33 58 34 714.6 0 34 59 34 714.6 0 34 60 27 714.6 0 27 61 29 714.6 0 29 62 47 714.6 0 47 63 25 714.6 0 25 64 35 714.6 0 35 65 33 714.6 0 33 66 48 714.6 0 48 67 31 714.6 0 31 68 37 714.6 0 37 69 35 714.6 0 35 70 34 714.6 0 34 71 120 714.6 0 120 72 47 714.6 0 47 73 38 714.6 0 38 74 38 714.6 0 38 75 41 714.6 0 41 76 44 714.6 0 44 77 39 714.6 0 39 78 36 714.6 0 36 79 59 714.6 0 59 80 25 714.6 0 25 81 61 714.6 0 61 82 127 714.6 0 127 83 67 714.6 0 67 84 108 714.6 0 108 85 164 714.6 0 164 86 111 714.6 0 111 87 73 714.6 0 73 88 80 714.6 0 80 89 88 714.6 0 88 90 96 714.6 0 96 91 116 714.6 0 116 92 115 714.6 0 115 93 115 714.6 0 115 94 321 714.6 0 321 95 193 714.6 0 193 96 225 714.6 0 225 97 227 714.6 0 227 98 180 714.6 0 180 99 439 714.6 0 439 100 163 714.6 0 163 101 83 714.6 0 83 102 138 714.6 0 138 103 111 714.6 0 111 104 81 714.6 0 81 105 41 714.6 0 41 106 92 714.6 0 92 107 106 714.6 0 106 108 171 714.6 0 171 109 119 714.6 0 119 110 171 714.6 0 171 111 99 714.6 0 99 112 142 714.6 0 142 113 94 714.6 0 94 114 147 714.6 0 147 115 276 714.6 0 276 116 219 714.6 0 219 117 172 714.6 0 172 118 172 714.6 0 172 119 253 714.6 0 253 120 287 714.6 0 287 121 248 714.6 0 248 122 148 714.6 0 148 123 172 714.6 0 172 124 231 714.6 0 231 125 362 714.6 0 362 126 227 714.6 0 227 127 313 714.6 0 313 128 220 714.6 0 220 129 251 714.6 0 251 130 256 714.6 0 256 131 235 714.6 0 235 132 166 714.6 0 166 133 220 714.6 0 220 134 544 714.6 0 544 135 751 714.6 0 751 136 549 714.6 0 549 137 505 714.6 0 505 138 292 714.6 0 292 139 220 714.6 0 220 140 250 714.6 0 250 141 252 714.6 0 252 142 205 714.6 0 205 143 206 714.6 0 206 144 224 714.6 0 224 145 715 714.6 0 715 146 670 714.6 0 670 147 653 714.6 0 653 148 280 714.6 0 280 149 222 714.6 0 222 150 222 714.6 0 222 151 265 714.6 0 265 152 302 714.6 0 302 153 381 714.6 0 381 154 428 714.6 0 428 155 417 714.6 0 417 156 380 714.6 0 380 157 455 714.6 0 455 158 389 714.6 0 389 159 365 714.6 0 365 160 304 714.6 0 304 161 277 714.6 0 277 162 270 714.6 0 270 163 242 714.6 0 242 164 319 714.6 0 319 165 920 714.6 0 920 166 758 714.6 0 758 167 436 714.6 0 436 168 295 714.6 0 295 169 260 714.6 0 260 170 285 714.6 0 285 171 262 714.6 0 262 172 338 714.6 0 338 173 1488 714.6 0 1488 174 2485 714.6 0 2485 175 1188 714.6 0 1188 176 624 714.6 0 624 177 242 714.6 0 242 178 261 714.6 0 261 179 363 714.6 0 363 180 501 714.6 0 501 181 1918 714.6 0 1918 182 793 714.6 0 793 183 752 714.6 0 752 184 700 714.6 0 700 185 405 714.6 0 405 186 796 714.6 0 796 187 455 714.6 0 455 188 254 714.6 0 254 189 241 714.6 0 241 190 340 714.6 0 340 191 620 714.6 0 620 192 806 714.6 0 806 193 838 714.6 0 838 194 486 714.6 0 486 195 213 714.6 0 213 196 215 714.6 0 215 197 320 714.6 0 320 198 545 714.6 0 545 199 385 714.6 0 385 200 278 714.6 0 278 201 368 714.6 0 368 202 465 714.6 0 465 203 489 714.6 0 489 204 647 714.6 0 647 205 464 714.6 0 464 206 479 714.6 0 479 207 452 714.6 0 452 208 343 714.6 0 343 209 313 714.6 0 313 210 319 714.6 0 319 211 369 714.6 0 369 212 497 714.6 0 497 213 424 714.6 0 424 214 658 714.6 0 658 215 636 714.6 0 636 216 540 714.6 0 540 217 535 714.6 0 535 218 626 714.6 0 626 219 775 714.6 0 775 220 611 714.6 0 611 221 622 714.6 0 622 222 575 714.6 0 575 223 539 714.6 0 539 224 518 714.6 0 518 225 3880 714.6 0 3880 226 4671 714.6 0 4671 227 7073 714.6 0 7073 228 566 714.6 0 566 229 375 714.6 0 375 230 983 714.6 0 983 231 971 714.6 0 971 232 1151 714.6 0 1151 233 611 714.6 0 611 234 865 714.6 0 865 235 647 714.6 0 647 236 528 714.6 0 528 237 607 714.6 0 607 238 585 714.6 0 585 239 559 714.6 0 559 240 452 714.6 0 452 241 518 714.6 0 518 242 547 714.6 0 547 243 535 714.6 0 535 244 458 714.6 0 458 245 477 714.6 0 477 246 1085 714.6 0 1085 247 1259 714.6 0 1259 248 886 714.6 0 886 249 205 714.6 0 205 250 667 714.6 0 667 251 1552 714.6 0 1552 252 2579 714.6 0 2579 253 1215 714.6 0 1215 254 1080 714.6 0 1080 255 714 714.6 0 714 256 649 714.6 0 649 257 566 714.6 0 566 258 521 714.6 0 521 259 347 714.6 0 347 260 412 714.6 0 412 261 509 714.6 0 509 262 555 714.6 0 555 263 480 714.6 0 480 264 477 714.6 0 477 265 539 714.6 0 539 266 957 714.6 0 957 267 740 714.6 0 740 268 344 714.6 0 344 269 320 714.6 0 320 270 479 714.6 0 479 271 821 714.6 0 821 272 758 714.6 0 758 273 595 714.6 0 595 274 624 714.6 0 624 275 689 714.6 0 689 276 543 714.6 0 543 277 618 714.6 0 618 278 867 714.6 0 867 279 1035 714.6 0 1035 280 1180 714.6 0 1180 281 1018 714.6 0 1018 282 791 714.6 0 791 283 657 714.6 0 657 284 750 714.6 0 750 285 1265 714.6 0 1265 286 1184 714.6 0 1184 287 664 714.6 0 664 288 411 714.6 0 411 289 475 714.6 0 475 290 1102 714.6 0 1102 291 1250 714.6 0 1250 292 1191 714.6 0 1191 293 619 714.6 0 619 294 522 714.6 0 522 295 588 714.6 0 588 296 751 714.6 0 751 297 892 714.6 0 892 298 1519 714.6 0 1519 299 2357 714.6 0 2357 300 4262 714.6 0 4262 301 28815 714.6 0 28815 Finished in 3.88 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 93,257 Reads with adapters: 37,581 (40.3%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 37,552 (40.3%) Total basepairs processed: 20,613,685 bp Total written (filtered): 10,361,961 bp (50.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 37581 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 13 0.0 3 0 0 13 16 250 0.0 3 0 248 2 17 37017 0.0 3 36544 466 7 18 68 0.0 3 4 60 3 1 19 21 0.0 3 1 0 15 5 20 156 0.0 3 139 3 1 13 26 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 36 1 0.0 3 1 37 1 0.0 3 1 45 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 49 5 0.0 3 0 0 0 5 55 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 1 129 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 153 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 187 13 0.0 3 0 0 0 13 246 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.31 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 37,552 Reads with adapters: 273 (0.7%) Reads written (passing filters): 37,552 (100.0%) Total basepairs processed: 10,361,961 bp Total written (filtered): 10,361,137 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 273 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.8% C: 31.9% G: 31.1% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 270 586.8 0 270 4 1 146.7 0 1 5 2 36.7 0 2